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| Silva Júnior, Jessé Marques da; Universidade Federal de Lavras - UFLA; Paiva, Renato; Universidade Federal de Lavras - UFLA; Campos, Ana Carolina Atala Lombelo; Universidade Federal de Lavras - UFLA; Rodrigues, Marcelo; Universidade Federal de Lavras - UFLA; Carvalho, Milene Alves de Figueiredo; Universidade Federal de Lavras - UFLA; Otoni, Wagner Campos; Universidade Federal de Viçosa - UFV. |
| The technique of hybridization using plant protoplasts is widely used in plant breeding programs. The purpose of our study is to further characterize the process of protoplast isolation from the ornamental species Etlingera elatior (Jack) R. M. Smith. Protoplasts were isolated from different tissues: in vitro leaves, in vitro pseudostem, and leaves from plants cultivated hydroponically. We tested six enzymatic combinations, four incubation time periods, the rotary system (40 rpm) or steady in the dark, and three concentrations of mannitol (0.5, 0.6 and 0.7 M). The diameter and viability of obtained protoplasts were evaluated. The best source of explants used for protoplast isolation was the in vitro leaves, which yielded 22x105 protoplasts g-1 of fresh... |
| Tipo: Pesquisa Empírica |
Palavras-chave: Fisiologia Vegetal; Biotecnologia Vegetal; Melhoramento Genético FDA; Ornamental plant; Enzymatic combinations; Incubation period Melhoramento Vegetal. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12309 |
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