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	Provedor de dados OAK (1) ArchiMer (1) BJMBR (1) BJM (1) BJPS (1) Autor Cheng,Kai (1) Compere, Chantal (1) Gong,Xianfeng (1) Hashimoto, Makoto (1) Hatanaka, Yasumaru (1) Lazerges, Mathieu (1) Macedo,Gabriela Alves (1) Macedo,Juliana Alves (1) Perrot, Hubert (1) Rabehagasoa, Niriniony (1) Sun,Shaoping (1) Suzuki,Gaby Tiemi (1) Mais... Palavra-chave Biotin (5) Albumin/nanoparticles/characterization (1) Alexandrium Minutum (1) Antiproliferative activities (1) Avidin (1) Biosensor (1) Biotinylated polypeptides (1) Candida sp (1) DNA (1) Diazirine (1) Docetaxel (1) Isoelectric focusing (1) Media development (1) Photoaffinity labeling (1) Quartz crystal miocrobalance (1) Riboflavin (1) Schistosoma mansoni (1) Self-assembled monolayer (1) Thiol (1) Vitamin production (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Info:eu-repo/semantics/other (1) Text (1) Ano 2018 (1) 2012 (1) 2011 (1) 2006 (1) 1997 (1) País Brazil (3) France (1) Japan (1) Idioma Inglês (5)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 5 Primeira ... 1 ... Última Thiol- and Biotin-Labeled Probes for Oligonucleotide Quartz Crystal Microbalance Biosensors of Microalga Alexandrium Minutum ArchiMer Lazerges, Mathieu; Perrot, Hubert; Rabehagasoa, Niriniony; Compere, Chantal. Two quartz crystal microbalance oligonucleotide biosensors of a toxic microalga gene sequence (Alexandrium Minutum) have been designed. Grafting on a gold surface of 20-base thiol- or biotin-labeled probe, and selective hybridization with the complementary 20-base target, have been monitored in situ with a 27 MHz quartz crystal microbalance under controlled hydrodynamic conditions. The frequency of the set up is stable to within a few hertz, corresponding to the nanogram scale, for three hour experiments. DNA recognition by the two biosensors is efficient and selective. Hybridization kinetic curves indicate that the biosensor designed with the thiol-labeled probe is more sensitive, and that the biosensor designed with the biotin-labeled probe has a shorter... Tipo: Text Palavras-chave: Alexandrium Minutum; Avidin; Biosensor; Biotin; DNA; Quartz crystal miocrobalance; Self-assembled monolayer; Thiol. Ano: 2012 URL: http://archimer.ifremer.fr/doc/00107/21810/19385.pdf Positively coded photoaffinity label for altering isoelectric points of proteins OAK Hashimoto, Makoto; Hatanaka, Yasumaru. Novel diazirinyl photoaffinity ligand, which contains (3-trifluoromethyl) phenyldiazirine and penta(epsilon-Boc-Lys) as a photoreactive code, allows the introduction of a positive cascade to alter the pI value of labeled components, facilitating the isolation of photolabeled biocomponents with isoelectric focusing techniques. (c) 2006 Elsevier Ltd. All rights reserved. Palavras-chave: Photoaffinity labeling; Diazirine; Isoelectric focusing; Biotin. Ano: 2006 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1321 Ultrastructural and biochemical detection of biotin and biotinylated polypeptides in Schistosoma mansoni BJMBR Biotinylation is proposed for the identification of surface proteins in Schistosoma mansoni using the streptavidin-HRP conjugate for the detection of labeled polypeptides. However, control samples also showed several endogenous biotinylated polypeptides. In an attempt to determine the possibility of nonspecific binding between the streptavidin-HRP conjugate and polypeptides from S. mansoni, the conjugate was blocked with biotinamidecaproate-N-hydroxysuccinimide ester (BcapNHS) before biotin-streptavidin blotting. No bands were detected on the nitrocellulose sheet, demonstrating the specific recognition of biotin by the streptavidin present in the conjugate. Whole cercariae and cercarial bodies and tails showed several endogenous biotinylated polypeptides.... Tipo: Info:eu-repo/semantics/other Palavras-chave: Biotin; Biotinylated polypeptides; Schistosoma mansoni. Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700003 Medium composition influence on Biotin and Riboflavin production by newly isolated Candida sp BJM Suzuki,Gaby Tiemi; Macedo,Juliana Alves; Macedo,Gabriela Alves. Complex B vitamins as Biotin and Riboflavin are required by living organisms, not only for growth but also for metabolite production, and the feed market classifies them as growth promoters. Since Brazil will soon be one of the world's biggest animal protein producers, feed production is a large consumer of vitamins and micronutrients. The industry requires 10 mg riboflavin/0.2 mg biotin per kilogram of feed; a ratio of 40 ~ 50:1. Although few studies have been conducted specifically on riboflavin production using factorial design and surface response method as an optimization strategy, it is a common practice in biotechnology with many research reports available. However, there are no reports on the use of statistical design for biotin production. This... Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida sp; Riboflavin; Biotin; Media development; Vitamin production. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300030 Preparation, characterization, and antiproliferative activities of biotin-decorated docetaxel-loaded bovine serum albumin nanoparticles BJPS Cheng,Kai; Sun,Shaoping; Gong,Xianfeng. ABSTRACT The aim of the present study was to characterize biotin-decorated docetaxel-loaded bovine serum albumin nanoparticles (DTX-BIO-BSA-NPs) and evaluate their antiproliferative activity in vitro. The particle size of prepared DTX-BIO-BSA-NPs was found to be always lower than 200 nm, with sizes of 166.9, 160.3, 159.0, 176.1 and 184.8 nm and the zeta potential was -29.51, -28.54, -36.54, -36.08 and -27.56 mV after redissolution with water for 0, 1, 2, 4 and 8 hours, respectively. The polydispersity index (PDI) was stable in the range of 0.170 - 0.178. In the in vitro drug-release study, the DTX-BIO-BSA-NPs targeted a human breast cancer cell line MCF-7 effectively. The x-ray diffraction spectrum and DSC curve of DTX-BIO-BSA-NPs suggested that docetaxel... Tipo: Info:eu-repo/semantics/article Palavras-chave: Albumin/nanoparticles/characterization; Docetaxel; Antiproliferative activities; Biotin. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000200617 Registros recuperados: 5 Primeira ... 1 ... Última

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