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| Salles, Ecléia Alexandra Poltronieri Buda; Alcantara, Giovana Bomfim; Quoirin, Marguerite Germaine Ghislaine; Gonçalves, Antonio Natal; Higa, Antonio Rioyei. |
| Due to low rates of rooting it is difficult to propagate selected clones of Acacia mearnsii by cuttings. The purpose of this study was to define a protocol for disinfection of nodal segments for in vitro introduction, aiming to improve the species genetically for forest plantation. Combination of different products concentration (70% ethyl alcohol, sodium hypochlorite, plant preservative mixture and mercuric chloride), immersion period and size of nodal segment were tested. The best disinfection treatment was the combination of 70% ethanol, 0.4% mercuric chloride, sodium hypochlorite at 2% in nodal segments of 10 mm, with 80% survival. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Black wattle; Clones; Cutting Engenharia Florestal; Silvicultura clonal; Propagação vegetativa Acacia negra; Clones; Estaquia. |
| Ano: 2017 |
URL: http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/1392 |
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| Quoirin,Marguerite; Bona,Cleusa; Souza,Elizandra F. de; Schwartsburd,Pedro B.. |
| Plantlets of blackwattle (Acacia mearnsii De Wild) were inoculated with the bacterium Rhodococcus fascians and cultured in vitro. Leafy galls appeared at the cotyledonary nodes in 75% of the infected plants. The galls were separated from the plants and cultured on a medium containing three-quarters-strength MS salts (Murashige and Skoog, 1962), MS vitamins, 2% sucrose and an antibiotic (cephalothin), supplemented with or without 0.2% activated charcoal. Histological studies conducted from the sixth to the twenty-second day after plant infection revealed the presence of newly formed meristematic centers, first in the axillary region, then on the petioles and lamina of the leaflets around the apical meristem. Approximately 37% of the galls developed one... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Black wattle; Legume tree; Micropropagation. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300002 |
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