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Registros recuperados: 74 | |
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Morga, Benjamin; Arzul, Isabelle; Faury, Nicole; Segarra, Amelie; Chollet, Bruno; Renault, Tristan. |
Bonamiosis due to the parasite Bonamia ostreae is a disease affecting the flat oyster Ostrea edulis. B. ostreae is a protozoan, affiliated to the order of haplosporidia and to the cercozoan phylum. This parasite is mainly intracellular, infecting haemocytes, cells notably involved in oyster defence mechanisms. Suppression subtractive hybridisation (SSH) was carried out in order to identify oyster genes differentially expressed during an infection of haemocytes with B. ostreae. Forward and reverse banks allowed obtaining 1104 and 1344 clones respectively, among which 391 and 480 clones showed a differential expression between both tested conditions (haemocytes alone versus haemocytes in contact with parasites). ESTs of interest including genes involved in... |
Tipo: Text |
Palavras-chave: Bonamia ostreae; Protozoan; Ostrea edulis; Flat oyster; Haemocytes; Gene expression; Suppression subtractive hybridisation. |
Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00018/12879/9828.pdf |
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Touzeau, Damien. |
L'ostréiculture représente une part économique régionale et française importante avec une production d'environ 117 106 Tonnes de coquillages par an (FAO, 2003). Cependant le développement de cette activité peut être limité par des mortalités, parfois massives, dues à des maladies infectieuses. L'huître plate Ostrea edulis a subi deux maladies majeures : la Marteiliose due à Marteilia refringens et la Bonamiose due à Bonamia ostreae. Ces maladies à déclaration obligatoires ont fait chuter la production de 20 000 tonnes dans les années 70 à 2 098 Tonnes aujourd'hui. Suite à ces mortalités, différents programmes de recherche ont été lancés par Ifremer pour tenter de préserver voire de relancer la culture de l'huître plate. Un de ces programmes concerne la... |
Tipo: Text |
Palavras-chave: Huître plate; Ostrea edulis; Parasite; Bonamia ostreae; Technique PCR; ARN 18 S; Diagnostic. |
Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/00034/14478/11777.pdf |
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Robert, Rene; Pichot, Yves; Comps, Michel. |
From 1988 to 1989, a study has been conducted pertaining to the growth of the flat oyster Ostrea edulis, as well as Marteilia refringens and Bonamia ostreae outbreaks. Commercial mono specific farming (flat oysters), with low density or mixed (flat and hollow oysters) along with higher density, has been taking place in the Arcachon basin oceanographic sectors, where main hydrobiological parameters were acquired simultaneously. The excellent growth of the flat oyster was witnessed in mono specific farming. However this growth was average in mixed farming, probably due to higher total oyster density and interspecies competition. Regardless of the type of farming, Marteilia refringens infection was very quick and may have been related to climatic bay water... |
Tipo: Text |
Palavras-chave: Basin of Arcachon; Pathology; Bonamia ostreae; Marteilia refringens; Growth; Ostrea edulis; Bassin d'Arcachon; Pathologie; Bonamia ostreae; Marteilia refringens; Croissance; Ostrea edulis. |
Ano: 1990 |
URL: http://archimer.ifremer.fr/doc/1990/rapport-1592.pdf |
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Haure, Joel; Nourry, Max; Le Bayon, Nicolas; Baud, Jean-pierre. |
Un contrôle des performances de souches d'huîtres plates, Ostrea edulis, issues d'un plan de sélection a été réalisé d'avril à novembre 1995 en élevage intensif. Un débit d'eau de mer fixé à 4l/heure/huître et un apport nutritif (Skeletonema costatum) de 3.10(9) cellules/jour/huître semblent constituer un bon compromis pour satisfaire les exigences physiologiques de l'huître plate. Les lots d'huîtres résistantes ou tolérantes à Bonamia ostreae présentent de meilleures performances de croissance que les lots témoins. Une hiérarchisation de la croissance validée sur le plan statistique a été mise en évidence comme suit : R6 (F0*F1) > R7 (F1*F2) > Q (lot témoin). La plupart des huîtres ont été infestées par le ver Polydora, malgré le traitement à la... |
Tipo: Text |
Palavras-chave: Génétique quantitative; Ostrea edulis; Bonamia ostreae; Souche résistante. |
Ano: 1996 |
URL: http://archimer.ifremer.fr/doc/00048/15875/13297.pdf |
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Boulo, Viviane; Hervio, Dominique; Morvan, Annie; Bachere, Evelyne; Mialhe, Eric. |
The chief importance of hemocytes as immune effectors in molluscs has led to develop researches for in vitro culture. The chemiluminescence technique has been used to estimate the phagocytosis capacity of Crassostrea gigas hemocytes according to different culture media and times. Sea water was used as a basic medium. Additives, such as antibiotics or glucose and buffers, such as Tris or Hepes, were tested as weIl as artificial sea water. Hemocyte chemiluminescence activities were recorded at t=o in relation to the medium composition. The highest values were observed when hemocytes were kept in sea water without any modification. Whichever medium, chemiluminescence activity was substantially decreased when hemocytes were previously kept in vitro for a few... |
Tipo: Text |
Palavras-chave: Crassotrea gigas; Oyster; Bonamia ostreae; Pathogens; Molluscs; Hemocytes. |
Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/acte-4115.PDF |
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Lallias, Delphine; Arzul, Isabelle; Heurtebise, Serge; Ferrand, Sylvie; Chollet, Bruno; Robert, Maeva; Beaumon, Andy; Boudry, Pierre; Morga, Benjamin; Lapegue, Sylvie. |
Bonamiosis is a parasitic disease (causative agent: Bonamia ostreae) affecting the European flat oyster Ostrea edulis, responsible for a drastic decline in its aquaculture production. Selective breeding programs for resistance to bonamiosis have been undertaken to counter this disease. In the present study, a 6-month cohabitation challenge experiment was performed in order to transmit the disease from wild oysters injected with the parasite to two tested families of oysters (20 and 8-month old at the beginning of the experiment, with different genetic backgrounds) originating from a selective breeding program developed by IFREMER in France. Mortalities were checked daily and ventricular heart smears were performed on dying or moribund oysters to detect the... |
Tipo: Text |
Palavras-chave: Heart smear; Transmission; Cohabitation experiment; Bonamia ostreae; Ostrea edulis. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4783.pdf |
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Martin, Anne-genevieve; Gerard, Andre; Cochennec, Nathalie; Langlade, Aime. |
In 1989, a selected strain of flat oyster. Ostrea edulis, was produced from breeders that survived Bonamia ostreae and that were over selected by injection of purified parasites. This generation (G 1) was compared to native oysters which had settled during the same year. After a growing period of 21 months, the oysters were tested according to three procedures: exposure to natural infection in an intertidal area, maintenance at the laboratory in tanks near heavily infested oysters, injection of parasites. The test lasted 7 months. In each procedure, oysters of the first generation (G1) showed a better survival rate than control oysters (72 to 94% versus 48 to 66%). On the other hand, the highest mortalities were observed among injected oysters (G1 and... |
Tipo: Text |
Palavras-chave: Genetics; Ostrea edulis; Bonamia ostreae; Parasite resistance. |
Ano: 1993 |
URL: http://archimer.ifremer.fr/doc/1993/acte-3201.pdf |
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Morga, Benjamin; Arzul, Isabelle; Chollet, Bruno; Gagnaire, Beatrice; Renault, Tristan. |
Bonamiosis is a disease affecting the flat oyster Ostrea edulis and caused by the protozoa parasite Bonamia ostreae, which is affiliated to the haplosporidia order and the cercozoa phylum. This parasite is mainly intracellular, infecting haemocytes, circulating cells playing an important role in the oyster's defence mechanisms. Even though the parasite's cycle has not been completely understood yet, direct infection from infected oysters to healthy oysters is possible. This leads us to think that there is no intermediary host in the cycle. Very little data on the parasite's infection mechanisms or the host's defence mechanisms is available. In order to better comprehend the Bonamia ostreae / host interaction, some flat oyster's haemocytes have been put in... |
Tipo: Text |
Palavras-chave: Hémocytes; Ostrea edulis; Bonamia ostreae; Parasite; Huîtres. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3368.pdf |
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Launey, Sophie; Barre, Marc; Gerard, Andre; Naciri, Yamama. |
Genetic variability at five microsatellite loci was analysed in three hatchery-propagated populations of the flat oyster, Ostrea edulis. These populations were part of a selection programme for resistance to the protozoan parasite Bonamia ostreae and were produced by mass spawns, without control of the genealogy. Evidence for population bottlenecks and inbreeding was sought. A reduction in the number of alleles, mainly due to the loss of rare alleles, was observed in all selected populations, relative to the natural population from which they were derived. Heterozygote excesses were observed in two populations, and were attributed to substructuring of the population into a small number of families. Pedigree reconstruction showed that these two populations... |
Tipo: Text |
Palavras-chave: Inbreeding; Effective population size; Bonamia ostreae; Ostrea edulis; European flat oyster. |
Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-2703.pdf |
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Grizel, Henri; Mialhe, Eric; Chagot, Dominique; Boulo, Viviane; Bachere, Evelyne. |
Research on bonamiasis, an epizootic disease of the edible oyster Ostrea edu/is caused by the protozoan Bonamia ostreae, is discussed in relation to oyster fanning, research technology, epizootiology, and management. Morphological and infectious characteristics of the parasite are described. Recent progress in isolation and purification of the parasite have permitted investigations into host defense mechanisms, parasite infectivity, and the development of a mol1usc-pathogen mode!. |
Tipo: Text |
Palavras-chave: Epizootic disease; Pathology; Protozoan; Bonamia ostreae; Oysters. |
Ano: 1988 |
URL: http://archimer.ifremer.fr/doc/1988/publication-3120.pdf |
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Registros recuperados: 74 | |
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