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Registros recuperados: 36
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High levels of chromosomal differentiation in Euchroma gigantea L. 1735 (Coleoptera, Buprestidae) Genet. Mol. Biol.
Moura,Rita de Cássia de; Melo,Natoniel Franklin de; Souza,Maria José de.
Euchroma gigantea was karyotypically studied using conventional staining, C-banding, silver nitrate staining and ribosomal fluorescent in situ hybridization (rDNA FISH). Broad wide autosomal polymorphism and a complex sex determination system were found in this beetle. Karyotype complements ranging from 2n = 32, X1X2X3Y1Y2Y3 to 2n = 36,X1X2X3Y1Y2Y3 were detected in the sample analyzed. Punctiform supernumerary chromosomes were present in the different karyotypes. The karyotypic evolution of Brazilian E. gigantea may have taken two directions, reduction in the diploid number of 2n = 36 to 24 through centric fusions or 2n = 24 to 36 due to chromosomal fissions. In addition, pericentric inversions were also involved. The complex multiple sex mechanism of this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome polymorphism; C-banding; Multiple sex system; NOR; RDNA.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300007
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Cytogenetic analysis of five species of the subfamily Corydoradinae (Teleostei: Siluriformes: Callichthyidae) Genet. Mol. Biol.
Shimabukuro-Dias,Cristiane Kioko; Oliveira,Claudio; Foresti,Fausto.
In the present study, five callichthyid species belonging to the subfamily Corydoradinae were karyotyped: three species of Aspidoras and two of Corydoras. The three species of Aspidoras had the same diploid number, 2n = 46 chromosomes, similar karyotypic formulae, with most chromosomes metacentric or submetacentric, single interstitial Ag-NORs and C-band positive segments mainly found in the centromeric position. The comparative analysis of cytogenetic data available for the genus Aspidoras and other species of Corydoradinae suggest that several events of centric fusion occurred in the origin of the species of Aspidoras. The two analyzed species of Corydoras showed high diploid numbers, 2n = 74 in C. sodalis and 2n = 90 in C. britskii. While C. sodalis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish cytogenetic; Karyotype; NOR; C-banding; Karyotypic evolution.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400014
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Cytogenetic data of Partamona peckolti (Hymenoptera, Apidae, Meliponini) by C banding and fluorochrome staining with DA/CMA3 and DA/DAPI Genet. Mol. Biol.
Brito,Rute Magalhães; Caixeiro,Ana Paula de Almeida; Pompolo,Silvia das Graças; Azevedo,Giselle Garcia.
The stingless bees of the Partamona genus have been studied taxonomically, ecologically and behaviourally, but cytogenetic studies are still rare. The objective of this study was to obtain cytogenetic data to contribute to Partamona peckolti species characterization. Heterochromatin was localized in all chromosome pericentromeric regions but some blocks could be visualized on some large chromosomes arms. A large heterozygous DA-CMA3-positive band was observed on one large chromosome arm, but was completely absent when C banding was applied before fluorochrome staining, with only one small positive band being visualized. Sequential DA-CMA3-NOR staining of interphase nuclei provided coincident positive responses. This suggests that DA-CMA3-positive bands of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Meliponini; Partamona; Karyotype; C-banding; Fluorochromes.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100009
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Chromosomal distribution of the As51 satellite DNA in two species complexes of the genus Astyanax (Pisces, Characidae) Genet. Mol. Biol.
Abel,Luciano Douglas dos Santos; Mantovani,Monique; Moreira-Filho,Orlando.
The chromosomal localization of the As51 satellite DNA was identified by fluorescent in situ hybridization (FISH) in specimens of the characid fish Astyanax scabripinnis and Astyanax fasciatus, which are considered species complexes because of their extensive karyotypical and morphological variability. A conserved chromosomal distribution of the As51 satellite, coincident with distal C-banded segments was demonstrated. The alternative interstitial localization of this satellite DNA and possible alterations of its structure suggest that this sequence underwent quantitative, positional and structural variations, as the A. scabripinnis and A. fasciatus complexes diverged.
Tipo: Info:eu-repo/semantics/article Palavras-chave: As51; Astyanax scabripinnis; Astyanax fasciatus; Fluorescence in situ hybridization (FISH); C-banding; Satellite DNA.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300008
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Cytogenetic characterization of two species of Frieseomelitta Ihering, 1912 (Hymenoptera, Apidae, Meliponini) Genet. Mol. Biol.
Carvalho,Antônio F.; Costa,Marco Antônio.
The cytogenetic analysis of Frieseomelitta dispar and F. francoi revealed the chromosome numbers 2n = 30 and n = 15 and a karyotypic formula 2K = 4M+2Mt+4A+20A M. The number of chromosomes observed was consistent with those reported for other Frieseomelitta species. The occurrence of the Mt chromosome and other features of the karyotype formulae suggest a close relationship between F. dispar, F. francoi and F. varia. Nevertheless, it was possible to differentiate the karyotypes of the species by DAPI/CMA3 staining, which revealed GC-rich regions on two chromosome pairs of F. dispar: one acrocentric and one pseudoacrocentric. In F. francoi, the same kinds of regions were observed on a pair of metacentrics and on a pair of acrocentrics. Our analysis also...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hymenoptera; Frieseomelitta; Interspecific differentiation; C-banding; Heterochromatin.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200012
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The karyotype of three Brazilian Terrarana frogs (Amphibia, Anura) with evidence of a new Barycholos species Genet. Mol. Biol.
Siqueira,Sérgio; Aguiar Jr.,Odair; Pansonato,André; Giaretta,Ariovaldo A.; Strüssmann,Christine; Martins,Itamar; Recco-Pimentel,Shirlei M..
A recent substantial rearrangement of the 882 described eleutherodactyline frog species has considerably improved the understanding of their systematics. Nevertheless, many taxonomic aspects of the South American eleutherodactyline species remain unknown and require further investigation using morphological, cytogenetic and molecular approaches. In this work, the karyotypes of the Brazilian species Ischnocnema juipoca (Atibaia and Campos do Jordão, SP), Barycholos cf. ternetzi (Uberlândia, MG, and Porto Nacional, TO), and Pristimantis crepitans (Chapada dos Guimarães and São Vicente, MT) were analyzed using Giemsa staining, Ag-NOR labeling, and C-banding techniques. All individuals had a diploid number of 22 chromosomes, but the Fundamental Numbers were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytogenetics; Barycholos; Ischnocnema; Pristimantis; Ag-NOR; C-banding.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300008
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Karyotypic conservatism in five species of Prochilodus (Characiformes, Prochilodontidae) disclosed by cytogenetic markers Genet. Mol. Biol.
Voltolin,Tatiana Aparecida; Penitente,Manolo; Mendonça,Bruna Bueno; Senhorini,José Augusto; Foresti,Fausto; Porto-Foresti,Fábio.
The family Prochilodontidae is considered a group with well conserved chromosomes characterized by their number, morphology and banding patterns. Thence, our study aimed at accomplishing a cytogenetic analysis with conventional methods (Giemsa staining, silver staining of the nucleolus organizer regions-AgNOR, and C-banding) and fluorescence in situ hybridization (FISH) with 18S and 5S ribosomal DNA probes in five species of the Prochilodus genus (Prochilodus argenteus, Prochilodus brevis, Prochilodus costatus, Prochilodus lineatus and Prochilodus nigricans) collected from different Brazilian hydrographic basins. The results revealed conservatism in chromosome number, morphology, AgNORs 18S and 5S rDNAs location and constitutive heterochromatin...
Tipo: Info:eu-repo/semantics/article Palavras-chave: AgNOR; C-banding; Hydrographic basins; Conserved karyotype; FISH.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300008
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C-banding and fluorescent in situ hybridization with rDNA sequences in chromosomes of Cycloneda sanguinea Linnaeus (Coleoptera, Coccinellidae) Genet. Mol. Biol.
Maffei,Eliane Mariza Dortas; Pompolo,Silvia das Graças; Petitpierre,Eduard.
The aim of this study was to describe mitotic and meiotic chromosomes of Cycloneda sanguinea using C-banding, fluorescent in situ hybridization (FISH) rDNA probes, and sequential FISH/Ag-NOR staining. The chromosome number was 2n = 18 + XX for females and 2n = 18 + Xy for males. The X chromosome was metacentric and the Y chromosome was very small. During meiosis, the karyotypic meioformula was n = 9 + Xy p, and sex chromosomes configured a parachute at metaphase I. At the beginning of pachytene, bivalents were still individualized, and sex chromosomes were associated end-to-end through the heteropycnotic region of the X chromosome. Later in pachytene, further condensation led to the formation of a pseudo-ring by the sex bivalent. All chromosomes showed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: In situ hybridization; RDNA sequences; Sequential FISH/Ag-NOR staining; C-banding; Cycloneda sanguinea.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200011
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Karyological analysis of Proechimys cuvieri and Proechimys guyannensis (Rodentia, Echimyidae) from central Amazon Genet. Mol. Biol.
Silva,Carlos Eduardo Faresin e; Eler,Eduardo Schmidt; Silva,Maria Nazareth F. da; Feldberg,Eliana.
The aim was to characterize the karyotype of rodents of the genus Proechimys from three localities in the central Brazilian Amazon, in the search for new markers that might shed light on our understanding of the taxonomy and evolutionary history of this taxon. Two karyotypes were found, viz., 2n = 28, FN = 46 in individuals from the NRSP (Cuieiras River) and REMAN (Manaus), and 2n = 46, FN = 50 in individuals from the Balbina Hydroelectric Plant. While individuals with the karyotype with 2n = 28 chromosomes were morphologically associated with Proechimys cuvieri, their karyotype shared similarities with those of the same diploid number in two other regions. Although three karyotypes are described for Proechimys cuvieri, no geographic distribution pattern...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spiny rats; C-banding; Amazon region; Karyotypes; FISH.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100012
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Intra- and inter-individual chromosome variation in Hoplerythrinus unitaeniatus (Pisces, Erythrinidae): a population from the Brazilian São Francisco river basin Genet. Mol. Biol.
Diniz,Débora; Bertollo,Luiz A.C..
Chromosomal studies were carried out on a population of the fish Hoplerythrinus unitaeniatus (jeju) from Prata river in the São Francisco river basin, Minas Gerais, Brazil using conventional Giemsa staining, C-banding, silver nitrate nucleolar organizer region (Ag-NOR) staining and fluorescent in situ hybridization (FISH) with 18S and 5S rDNA probes. We found a high degree of inter- and intra-individual variability with the identification of 2n = 50, 2n = 51 and 2n = 52 karyotypes at nearly the same frequency within the population. Intra-individual variation in chromosomal morphology and, consequently, karyotype formulae was also observed, chiefly in the specimens with 2n = 50 and 2n = 52 chromosomes. Ag-NORs and 18S rDNA sites also showed numerical and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Karyotypes; C-banding; Nucleolar organizing regions; Intra- and inter-individual polymorphism.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300009
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Genetic diversity and phylogeny in Hystrix (Poaceae, Triticeae) and related genera inferred from Giemsa C-banded karyotypes Genet. Mol. Biol.
Zhang,Hai-Qin; Yang,Rui-Wu; Zhang,Li; Ding,Chun-Bang; Zeng,Jian; Zhou,Yong-Hong.
The phylogenetic relationships of 15 taxa from Hystrix and the related genera Leymus (NsXm), Elymus (StH), Pseudoroegneria (St), Hordeum (H), Psathyrostachys (Ns), and Thinopyrum (E) were examined by using the Giemsa C-banded karyotype. The Hy. patula C-banding pattern was similar to those of Elymus species, whereas C-banding patterns of the other Hystrix species were similar to those of Leymus species. The results suggest high genetic diversity within Hystrix, and support treating Hy. patula as E. hystrix L., and transferring Hy. coreana, Hy. duthiei ssp. duthiei and Hy. duthiei ssp. longearistata to the genus Leymus. On comparing C-banding patterns of Elymus species with their diploid ancestors (Pseudoroegneria and Hordeum), there are indications that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: C-banding; Elymus; Genome; Hystrix; Leymus; Triticeae.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300015
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Cytogenetic studies of three Lycosidae species from Argentina (Arachnida, Araneae) Genet. Mol. Biol.
Chemisquy,María A.; Gil,Sergio G. Rodríguez; Scioscia,Cristina L.; Mola,Liliana M..
Cytogenetic studies of the family Lycosidae (Arachnida: Araneae) are scarce. Less than 4% of the described species have been analyzed and the male haploid chromosome numbers ranged from 8+X 1 X 2 to 13+X 1 X 2 . Species formerly classified as Lycosa were the most studied ones. Our aim in this work was to perform a comparative analysis of the meiosis in " Lycosa " erythrognatha Lucas, " Lycosa " pampeana Holmberg and Schizocosa malitiosa (Tullgren). We also compared male and female karyotypes and characterized the heterochromatin of " L. " erythrognatha . The males of the three species had 2n = 22, n = 10+X 1 X 2 , all the chromosomes were telocentric and there was generally a single chiasma per bivalent. In " Lycosa " pampeana , which is...
Palavras-chave: Meiosis; C-banding; Fluorochrome staining; Karyotype; Spiders; "Lycosa" and Schizocosa.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000500010
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Cytogenetic analysis of three catfish species of the family Pseudopimelodidae (Teleostei, Siluriformes) Genet. Mol. Biol.
Martinez,Emanuel Ricardo Monteiro; Nirchio,Mauro; Granado,Angel; Foresti,Fausto; Oliveira,Claudio.
Cytogenetic analyses performed in Cephalosilurus apurensis, Microglanis aff. cottoides and Pseudopimelodus bufonius revealed that the three species have 2n = 54 chromosomes: C. apurensis presented six metacentric (M), 28 submetacentric (SM), 14 subtelolocentric (ST), and six acrocentric (A) chromosomes, while M. aff. cottoides showed 10M, 32SM, 10ST and 2A, and P. bufonius had 12M, 30SM and 12ST. The nucleolus organizer regions (NORs) were present on the short arm of a middle-sized ST pair, identified as pair 19, in C. apurensis NORs were found on the short arm of a middle-sized ST (pair 23) and on the long arm of a middle-sized ST (pair 22) in M. aff. cotttoides and on the short arm of three middle-sized ST pairs, identified as pairs 9, 10 and 11, in P....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Karyotype; Chromosomes; C-banding; Ag-NOR; Fish.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400015
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Cytogenetic divergence between two sympatric species of Characidium (Teleostei, Characiformes, Crenuchidae) from the Machado River, Minas Gerais, Brazil Genet. Mol. Biol.
Silva,Alexandre Rodrigues da; Maistro,Edson Luis.
Cytogenetic studies were performed on two sympatric species of Characidium, C. gomesi and C. cf. zebra, from the Grande River basin, Minas Gerais State, Brazil. Although both species had a chromosome number of 50 with a karyotype exclusively consisting of meta- and submetacentric chromosomes, interspecific diversity was detected concerning the size of the two first chromosome pairs of the karyotypes. Active nucleolus organizer regions (NORs) were located at the terminal position on the long arm of the 17th pair of C. gomesi and at subterminal position on the long arm of the 23rd pair of C. cf. zebra. For both species the fluorochrome CMA3 stained only the NOR-bearing pair of chromosomes. The heterochromatin pattern also showed some differentiation between...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Characidium gomesi; Characidium cf. zebra; Crenuchidae; NOR-banding; C-banding.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300010
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Evolutionary dynamics of the karyotype of the wasp Trypoxylon (Trypargilum) nitidum (Hymenoptera, Sphecidae) from the Rio Doce State Park, Minas Gerais, Brazil Genet. Mol. Biol.
Scher,Ricardo; Pompolo,Silvia das Graças.
Cytogenetic analysis based on the distribution of C-bands showed two groups of karyotypes in a Trypoxylon nitidum population from the Rio Doce Park, State of Minas Gerais, Brazil. One of these groups, that was identical to a previously described karyotype (n = 15; 2n = 30), had a stable chromosome number and was rich in acrocentric chromosomes, whereas the other had a variable chromosome number (n = 12 to 14; 2n = 25 to 28) and was rich in pseudo-acrocentric chromosomes. We propose a hypothesis explaining the dynamics of the modifications which occurred in the karyotype of this species, based on the minimum interaction theory of Imai et al. (1986, 1988, 1994) and on the chromosome rearrangements and heteromorphisms observed by us.
Tipo: Info:eu-repo/semantics/article Palavras-chave: C-banding; Trypoxylon nitidum; Karyotype evolution.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300015
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Cytogenetic characterization of a population of Bryconamericus aff. iheringii (Characidae,Tetragonopterinae) Genet. Mol. Biol.
Paintner-Marques,Tânia Regina; Giuliano-Caetano,Lucia; Dias,Ana Lúcia.
A cytogenetic analysis of thirteen specimens of Bryconamericus aff. Iheringii revealed a diploid number of 52 chromosomes and a karyotype of 8M+22SM+10ST+12A with a fundamental number (FN) of 92. The nucleolus organizer regions (NORs) were studied by means of AgNO3 and CMA3 staining as well as by FISH using an 18S rDNA probe. NORs were found to be located at the terminal position on the short arm of the submetacentric chromosome pair. C-banding showed strong telomeric and centromeric staining in the majority of the chromosomes, and a similar pattern was observed after treatment with AluI restriction enzyme
Tipo: Info:eu-repo/semantics/article Palavras-chave: AgNOR; Alu I; Bryconamericus; C-banding; CMA3; RDNA 18S.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000200007
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Cytogenetic characterization of Melipona rufiventris Lepeletier 1836 and Melipona mondury Smith 1863 (Hymenoptera, Apidae) by C banding and fluorochromes staining Genet. Mol. Biol.
Lopes,Denilce Meneses; Pompolo,Sílvia das Graças; Campos,Lúcio Antônio de Oliveira; Tavares,Mara Garcia.
The stingless bees Melipona rufiventris and M. mondury were analyzed cytogenetically by conventional staining with Giemsa, C-banding and sequential staining with the fluorochromes CMA3/DA/DAPI. Both species presented 2n = 18 and n = 9, except for one colony of M. rufiventris, in which some individuals had 2n = 19 due to the presence of a B chromosome. After Giemsa staining and C-banding the chromosomes appeared very condensed and presented a high heterochromatic content, making it difficult to localize the centromere and therefore to visualize the chromosomes morphology. The constitutive heterochromatin was located in interstitial chromosome regions covering most of the chromosomes extension and consisted mainly of AT, as shown by DAPI staining. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Melipona; Stingless bees; B chromosomes; Fluorochromes; C-banding.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100010
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Karyotype and nuclear DNA content of hexa-, octo-, and duodecaploid lines of Bromus subgen. Ceratochloa Genet. Mol. Biol.
Klos,Joanna; Sliwinska,Elwira; Kula,Adam; Golczyk,Hieronim; Grabowska-Joachimiak,Aleksandra; Ilnicki,Tomasz; Szostek,Krzysztof; Stewart,Alan; Joachimiak,Andrzej J..
The subgenus Ceratochloa of the genus Bromus includes a number of closely related allopolyploid forms or species that present a difficult taxonomic problem. The present work combines data concerning chromosome length, heterochromatin distribution and nuclear genome size of different 6x, 8x and 12x accessions in this subgenus. Special attention is paid to the karyotype structure and genomic constitution of duodecaploid plants recently found in South America. Hexaploid lineages possess six almost indistinguishable genomes and a nuclear DNA content between 12.72 pg and 15.10 pg (mean 1Cx value = 2.32 pg), whereas octoploid lineages contain the same six genomes (AABBCC) plus two that are characterized by longer chromosomes and a greater DNA content (1Cx = 4.47...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Karyotype; C-banding; Heterochromatin; Flow cytometry; Genome size.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300016
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Identification and description of distinct B chromosomes in Cyphocharax modestus (Characiformes, Curimatidae) Genet. Mol. Biol.
Santos,Lessandra Viviane De Rosa; Foresti,Fausto; Martins,Cesar; Oliveira,Claudio; Wasko,Adriane Pinto.
Cytogenetic analyses were performed in Cyphocharax modestus, collected at Paranapanema River and Tietê River (São Paulo State, Brazil). A karyotype with 2n = 54 chromosomes was observed in the animals from both Brazilian freshwater river systems. One to four B chromosomes were also detected in individuals from the Paranapanema River, which represents the probable first report of more than a single supernumerary element in a species of the Curimatidae group. C-banding revealed centromeric and telomeric heterochromatin blocks in several chromosomes of the normal karyotype complement of C. modestus. Moreover, while some B chromosomes were characterized by the complete absence of C-bands, others were totally heterochromatic. Although there was a prevalence of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: B chromosomes; C-banding; Curimatidae; Cyphocharax modestus; Supernumerary chromosomes.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200019
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Cytogenetic analysis of three species of Pseudacteon (Diptera, Phoridae) parasitoids of the fire ants using standard and molecular techniques Genet. Mol. Biol.
Chirino,Mónica G.; Folgarait,Patricia J.; Gilbert,Lawrence E.; Lanzavecchia,Silvia; Papeschi,Alba G..
Pseudacteon flies, parasitoids of worker ants, are being intensively studied as potentially effective agents in the biological control of the invasive pest fire ant genus Solenopsis (Hymenoptera: Formicidae). This is the first attempt to describe the karyotype of P. curvatus Borgmeier, P. nocens Borgmeier and P. tricuspis Borgmeier. The three species possess 2n = 6; chromosomes I and II were metacentric in the three species, but chromosome pair III was subtelocentric in P. curvatus and P. tricuspis, and telocentric in P. nocens. All three species possess a C positive band in chromosome II, lack C positive heterochromatin on chromosome I, and are mostly differentiated with respect to chromosome III. P. curvatus and P. tricuspis possess a C positive band,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudacteon; Karyotype evolution; C-banding; Fluorescent banding; FISH.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400013
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