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Histo-cytological responses of the prolactin cells of the catfish Heteropneustes fossilis to cadmium exposure Acta Toxicol. Argent.
Srivastav,A.K; Rai,Rubi; Mishra,D; Srivastav,S. K; Suzuki,Nobuo.
Cadmium is an important metal for modern industrial processes and, being biologically non-essential, poses health hazards to the organisms. In this study we aimed to evaluate the effect of cadmium exposure on the histo-cytology of prolactin cells in the freshwater catfish, Heteropneustes (H.) fossilis. Fish were subjected to 288 mg/L (0.8 of 96 h LC50) and 72 mg/L (0.2 of 96 h LC50) of cadmium chloride for short-term and long-term, respectively. After sacrificing the fish, the blood was collected on 24, 48, 72 and 96 h in short-term and after 7, 14, 21, and 28 days in long-term experiment and analyzed for plasma calcium levels. Also, pituitary glands were fixed on these intervals. The plasma calcium levels of short-term cadmium exposed fish remain...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cadmium; Fish; Prolactin; Pituitary gland.
Ano: 2014 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-37432014000300002
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Uptake and translocation of Cd and Zn in two lettuce cultivars Anais da ABC (AABC)
FONTES,RENILDES L.F.; PEREIRA,JULIANA M.N.; NEVES,JULIO C.L..
Excess of heavy metals in agricultural soils is a matter of concern since it may decrease economic yield as a result of toxicity and lower product quality as a result of metal accumulation in edible plant parts. Among plant species and among cultivars within species a natural variation in uptake, translocation and distribution of trace elements occur. The transference of Cd and Zn, from soil to two lettuce (Lactuca sativa L.) cultivars grown in greenhouse, was evaluated in separate experiments for Cd and Zn.Plant dry and fresh matter yield and plant Cd and Zn concentrations were determined. Cultivar CRV showed greater potential for yield than CMM in both experiments. Cadmium and Zn translocation from roots to shoots increased with the increase of soil Cd...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cadmium; Food quality; Lettuce; Plant nutrition; Soil; Zinc.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000200907
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Application of modified simplex on the development of a preconcentration system for cadmium determination in sediments, food and cigarettes Anais da ABC (AABC)
SANTOS,QUEILA O.; MORENO,ISLANIA; SANTOS,LEANDRO DOS; SANTOS,ALDENOR G.; SOUZA,VALDINEI S.; BEZERRA,MARCOS A..
A modified simplex algorithm was used to optimize a system of preconcentration for cadmium determination in samples of sediments, cigarettes and food using flame atomic absorption spectrometry. The preconcentration system is based on the sorption of cadmium in a minicolumn packed with Amberlite XAD-2 resin functionalized with 3,4-dihydroxybenzoic acid (DHB). The optimized variables were pH and sampling flow rate and the optimum conditions found for these variables were, respectively 8.7 and 8.8 mL min-1. The developed system showed a preconcentration factor of 15.3, detection limit of 0.49 µg L-1, quantification limit of 1.65 µg L-1 and precision expressed as relative standard deviation (% RSD, n=10) of 5.9. The accuracy of the method was checked by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Simplex optimization; Preconcentration; Cadmium; Sediments; Cigarettes; Food.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000300791
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Associative diazotrophic bacteria in grass roots and soils from heavy metal contaminated sites Anais da ABC (AABC)
Moreira,Fátima M.S.; Lange,Anderson; Klauberg-Filho,Osmar; Siqueira,José O.; Nóbrega,Rafaela S.A.; Lima,Adriana S..
This work aimed to evaluate density of associative diazotrophic bacteria populations in soil and grass root samples from heavy metal contaminated sites, and to characterize isolates from these populations, both, phenotypically (Zinc, Cadmium and NaCl tolerance in vitro, and protein profiles) and genotypically (16S rDNA sequencing), as compared to type strains of known diazotrophic species. Densities were evaluated by using NFb, Fam and JNFb media, commonly used for enrichment cultures of diazotrophic bacteria. Bacterial densities found in soil and grass root samples from contaminated sites were similar to those reported for agricultural soils. Azospirillum spp. isolates from contaminated sites and type strains from non-contaminated sites varied...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Azospirillum; Zinc; Cadmium; Soil contaminants; Nitrogen-fixing bacteria.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652008000400014
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Assessment of heavy metal residues in Brazilian poultry and swine tissue Arq. Bras. Med. Vet. Zootec.
Alkmim Filho,J. F.; Germano,A.; Dibai,W.L.S.; Vargas,E.A.; Melo,M.M..
The aim of this study was to investigate the presence of arsenic, lead and cadmium residues in samples of liver, kidney and muscle of poultry and swine during the years from 2002 to 2008. A total of 1978 samples were analyzed: 1031 of poultry and 947 of swine from Brazilian slaughterhouses. The samples were analyzed at the National Agricultural Laboratory using the atomic absorption spectrometry technique. In poultry, the arsenic residues were detected in 53.6% of liver samples, although no results have exceeded the MRL. In kidneys, 39.7% of the samples showed measurable residues and there was no violation of the limits. Regarding lead, there were 5 contaminated liver samples and 24 contaminated kidney samples (1.5 and 3.6% respectively). In muscle tissue...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trace elements; Arsenic; Cadmium; Lead.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000200022
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Cadmium-induced apoptosis of Siberian tiger fibroblasts via disrupted intracellular homeostasis Biol. Res.
Wang,Hui; Liu,Zheng; Zhang,Wenxiu; Yuan,Ziao; Yuan,Hongyi; Liu,Xueting; Yang,Chunwen; Guan,Weijun.
BACKGROUND: Heavy metals can cause great harm to Siberian tigers in the natural environment. Cadmium (Cd2+) is an environmental contaminant that affects multiple cellular processes, including cell proliferation, differentiation, and survival. It has been shown to induce apoptosis in a variety of cell types and tissues. RESULTS: We investigated the apoptotic effects of Cd2+ on Siberian tiger fibroblasts in vitro. Our research revealed the typical signs of apoptosis after Cd²+ exposure. Apoptosis was dose- (0-4.8 μΜ) and duration-dependent (12-48 h), and proliferation was strongly inhibited. Cd²+ increased the activity of caspase-3, -8, and -9 and disrupted calcium homeostasis by causing oxidative stress and mitochondrial dysfunction. It...
Tipo: Journal article Palavras-chave: Siberian tiger; Cadmium; Apoptosis; Fibroblast; Calcium.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100042
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Positive effects of Green Tea on hepatic dysfunction, lipid peroxidation and antioxidant defence depletion induced by cadmium Biol. Res.
HAMDEN,KHALED; CARREA U,SERGE; AYADI MARKI,FATMA; MASMOUDI,HATEM; EL FEKI,ABDELFATTAH.
Cadmium (Cd) is a highly toxic environmental and industrial cumulative pollutant that affeets many organs, especially the liver. The present study was designed to evalúate the antioxidant effect of green tea on cadmium-induced hepatic dysfunction and oxidative stress in rats. Adult male Wistar rats were administered cadmium by injection of 20 emoles /kg bw/ every 3 days for six months. This study revealed significant (p < 0.05) liver dysfunction, lipid peroxidation and a decline in antioxidant enzyme activities in the liver of cadmium-treated rats compared to control animáis. Compared to control rats, the activities of lactate dehydrogenase (LDH), gammaglutamyl transferase (GGT), acid phosphatase (PAC), phosphatase alkaline (PAL), as well as bilirubin...
Tipo: Journal article Palavras-chave: Cadmium; Rat liver dysfunction; Green tea; Antioxidant enzymes; Lipid peroxidation.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300009
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Cadmium toxicity affects chlorophyll a and b content, antioxidant enzyme activities and mineral nutrient accumulation in strawberry Biol. Res.
Muradoglu,Ferhad; Gundogdu,Muttalip; Ercisli,Sezai; Encu,Tarik; Balta,Fikri; Jaafar,Hawa ZE; Zia-Ul-Haq,Muhammad.
BACKGROUND: Cadmium (Cd) is well known as one of the most toxic metals affecting the environment and can severely restrict plant growth and development. In this study, Cd toxicities were studied in strawberry cv. Camarosa using pot experiment. Chlorophyll and malondialdehyde (MDA) contents, catalase (CAT), superoxide dismutase (SOD), ascorbate peroxidase (APX) activities and mineral nutrient concentrations were investigated in both roots and leaves of strawberry plant after exposure Cd. RESULTS: Cd content in both roots and leaves was increased with the application of increasing concentrations of Cd. We found higher Cd concentration in roots rather than in leaves. Chlorophyll a and b was decreased in leaves but MDA significantly increased under increased...
Tipo: Journal article Palavras-chave: Antioxidant enzymes; Cadmium; Chlorophyll; Heavy metal stress; Strawberry.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100011
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Geobacillus stearothermophilus LV cadA gene mediates resistance to cadmium, lead and zinc in zntA mutants of Salmonella entérica serovar Typhimurium Biol. Res.
PÉREZ,JOSÉ M; PRADEÑAS,GONZALO A; NAVARRO,CLAUDIO A; HENRÍQUEZ,DANIEL R; PICHUANTES,SERGIO E; VÁSQUEZ,CLAUDIO C.
Salmonella entérica serovar Typhimurium cells expressing the cadA gene of Geobacillus stearothermophilus LV exhibit a hypersensitive phenotype to cadmium chloride. Deletion of the ORF STM3576 from the Salmonella genome resulted in cadmium, lead and zinc sensitivity, confirming that this ORF is a homologue of the zntA gene. The observed sensitivity was reverted upon expression of the G. stearothermophilus LV cadA gene. These results indicate that the cadA gene product is involved in Cd, Pb and Zn resistance as a classical P-type ATPase and strongly suggest that the observed hypersensitive phenotype to these metals can be related to the function of the host ·zntA gene product
Tipo: Journal article Palavras-chave: CadA; Cadmium; Lead; P-type ATPase; Zinc; ZntA.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500009
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Uptake of cadmium by Pseudokirchneriella supcapitata BABT
Casiraghi,Magela Paula; Luporini,Samuel; Silva,Eduardo Mendes da.
In this work the microalgae Pseudokirchneriella supcapitata was used for the removal of the cadmium in liquids. The accumulations of metal ions by the alga occur in two stages: a very fast absorption (passive adsorption) proceeded by a slower absorption (activate absorption). A mathematical model based on the surface absorption and on the transport into the interior of the cellular membrane was developed. The simulation model kinetic parameters were experimentally obtained. Through the results observed, the mathematical model was shown to be suitable when compared to the experimental results, confirming the validation of the mathematical model.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cadmium; Microalgae; Heavy metals; Mathematical model.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000800019
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Opiatergic participation in the thirst-inhibiting effect of acute third ventricle injections of cadmium (Cd2+) and lead (Pb2+) BJMBR
De-Castro-e-Silva,E.; Luz,C.P.; Sarmento,C.; Nascimento,T.; Gonzalez,V.; Marinho,C.A.; Castro,L.; Oliveira,P.; Santana Jr.,P.; De-Oliveira,I.R.; De-Paula,S.; Lima,A.K.S.; Fregoneze,J.B..
We have previously demonstrated that acute third ventricle injections of both lead and cadmium prevent the dipsogenic response elicited by dehydration or by central injections of dipsogenic agents such as angiotensin II, carbachol and isoproterenol in rats. We have also shown that the antidipsogenic action of cadmium may be due, at least in part, to activation of thirst-inhibitory central serotonergic pathways. In the present paper we show that in Wistar male rats the antidipsogenic effect of both lead acetate (3.0 nmol/rat) and cadmium chloride (3.0 nmol/rat) may be partially dependent on the activation of brain opiatergic pathways since central injections of naloxone (82.5 nmol/rat), a non-selective opioid antagonist, blunt the thirst-inhibiting effect...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Cadmium; Lead; Water intake; Opioids; Rats.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000600013
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Lead (Pb2+) and cadmium (Cd2+) inhibit the dipsogenic action of central beta-adrenergic stimulation by isoproterenol BJMBR
We have previously demonstrated that acute third ventricle injections of both Pb2+ and Cd2+ impair the dipsogenic response elicited by three different situations: dehydration and central cholinergic or angiotensinergic stimulation. ß-Adrenergic activation is part of the multifactorial integrated systems operating in drinking behavior control in the central nervous system. In the present study acute third ventricle injections of Pb2+ (3, 30 and 300 pmol/rat) or Cd2+ (0.3, 3 and 30 pmol/rat) blocked the dipsogenic response induced by third ventricle injections of isoproterenol (ISO; 160 nmol/rat) in a dose-dependent manner. Normohydrated animals receiving ISO + NaAc (sodium acetate) or saline (controls) displayed a high water intake after 120 min (ISO +...
Palavras-chave: Lead; Cadmium; Water intake; Isoproterenol.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000300018
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Acute and chronic effects of cadmium on blood homeostasis of an estuarine crab, Chasmagnathus granulata, and the modifying effect of salinity BJMBR
Rodríguez,E.M.; Bigi,R.; Medesani,D.A.; Stella,V.S.; Greco,L.S.L.; Moreno,P.A.R.; Monserrat,J.M.; Pellerano,G.N.; Ansaldo,M..
Whole body oxygen consumption and some hemolymph parameters such as pH, partial pressure of gases, level of ions and lactate were measured in the estuarine crab Chasmagnathus granulata after both acute (96 h) and chronic (2 weeks) exposure to cadmium at concentrations ranging from 0.4 to 6.3 mg/l. In all instances, the crabs developed hemolymph acidosis, but no respiratory (increased PCO2) or lactate increases were evident. Hemolymph levels of sodium and calcium were always increased by cadmium exposure. The chronic toxicity of cadmium was enhanced at 12‰ salinity, even causing a significantly higher mortality in comparison with the higher salinity (30‰) used. A general metabolic arrest took place at 12‰ salinity in the crabs chronically exposed to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cadmium; Crabs; Blood homeostasis; Salinity.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400011
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Adaptive and cross-protective responses against cadmium and zinc toxicity in cadmium-resistant bacterium isolated from a zinc mine BJM
Prapagdee,Benjaphorn; Watcharamusik,Anchulee.
Cadmium (Cd) is a major environmental hazard, which usually is detected in its ionic form of Cd2+. It also causes adverse toxic effects on human health and other living organisms. Cd-resistant bacteria were isolated from Cd-contaminated soils. One isolate, TAK1, was highly resistance level to Cd toxicity. TAK1 was isolated from soil contaminated with a high Cd concentration (204.1 mg.kg-1). The result of 16S rDNA sequence analysis found that the TAK1 showed the similarity to Ralstonia sp. Physiological adaptive and cross-protective responses to Cd and Zn killing were investigated in Ralstonia sp.TAK1. Exposure to a low concentration of Cd induced adaptive resistance to higher concentrations of Cd. In addition, pretreatment of Ralstonia sp.TAK1 with an...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial responses; Cadmium; Zinc; Metal Resistance; Ralstonia sp.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400014
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Bioemulsifier production by Microbacterium sp. strains isolated from mangrove and their application to remove cadmiun and zinc from hazardous industrial residue BJM
Aniszewski,Erick; Peixoto,Raquel Silva; Mota,Fábio Faria; Leite,Selma Gomes Ferreira; Rosado,Alexandre Soares.
The contamination of ecosystems with heavy metals is an important issue in current world and remediation technologies should be in according to environmental sustainability concept. Bioemulsifier are promising agents to be used in metal removal and could be effective to many applications in environmental industries. The aims of this work was screening the potential production of bioemulsifier by microorganisms isolated from an oil contaminated mangrove, and evaluate cadmium and zinc removal potential of those strains from a hazardous industrial residue. From that, bioemulsifier-producing bacteria were isolated from urban mangrove sediments. Four isolates were identified as Microbacterium sp by 16S rRNA analysis and were able to reduce up to 53.3% of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biosemulsifier; Heavy metals; Zinc; Cadmium; Microbacterium; Bioremediation.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100033
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Heterologous expression of a rice metallothionein isoform (OsMTI-1b) in Saccharomyces cerevisiae enhances cadmium, hydrogen peroxide and ethanol tolerance BJM
Ansarypour,Zahra; Shahpiri,Azar.
Abstract Metallothioneins are a superfamily of low-molecular-weight, cysteine (Cys)-rich proteins that are believed to play important roles in protection against metal toxicity and oxidative stress. The main purpose of this study was to investigate the effect of heterologous expression of a rice metallothionein isoform (OsMTI-1b) on the tolerance of Saccharomyces cerevisiae to Cd2+, H2O2 and ethanol stress. The gene encoding OsMTI-1b was cloned into p426GPD as a yeast expression vector. The new construct was transformed to competent cells of S. cerevisiae. After verification of heterologous expression of OsMTI-1b, the new strain and control were grown under stress conditions. In comparison to control strain, the transformed S. cerevisiae cells expressing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cadmium; Ethanol; Hydrogen peroxide; Rice metallothionein; S. cerevisiae.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300537
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Factors involved with cadmium absorption by a wild-type strain of Saccharomyces cerevisiae BJM
Adamis,Paula D.B.; Panek,Anita D.; Leite,Selma G.F.; Eleutherio,Elis C.A..
At the concentration used in this work (10 ppm), cadmium was efficiently removed from the environment by stationary yeast cells. While exponential phase cells showed low capacity of cadmium absorption, stationary cells removed 97% of the original metal in 24 hours. Total cadmium absorption shown by dry cells was lower than that of fresh ones, although both cells removed 50% of metal during the first hour of treatment. We also verified that only viable cells were capable of absorbing cadmium. Independently of the growth phase, cells showed high tolerance to 10 ppm CdSO4 and about 80% of cells remained viable after 24 hours exposure to cadmium. However, when stationary phase cells were previously dehydrated and then exposed to cadmium, they exhibited poor...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharomyces cerevisiae; Cadmium; Absorption; Intracellular oxidation.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100012
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Pseudomonas aeruginosa KUCd1, a possible candidate for cadmium bioremediation BJM
Sinha,Sangram; Mukherjee,Samir Kumar.
A cadmium (8 mM) resistant Pseudomonas aeruginosa strain KUCd1 exhibiting high Cd accumulation under in vitro aerobic condition has been reported. The isolate showed a significant ability to remove more than 75% and 89% of the soluble cadmium during the active growth phase from the growth medium and from Cd-amended industrial wastewater under growth supportive condition. Transmission electron microscopy (TEM) and energy dispersive X-ray spectroscopy (EDXS) suggest the presence of Cd in the cells from mid-stationary phase. The cell fractionation study revealed membrane and periplasm to be the major accumulating site in this strain. The chemical nature of the accumulated Cd was studied by X-ray powder diffraction analysis.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Cadmium; Bioremediation.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300030
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Interactive effects between supplemental ultraviolet-B radiation and heavy metals on the growth and biochemical characteristics of Spinacia oleracea L. Braz. J. Plant Physiol.
Shweta,Mishra; Agrawal,S.B..
The impact of supplemental UV-B (sUV-B) radiation and heavy metals: Cd (68 µmol.kg-1 soil) and Ni (68 µmol.kg-1 soil) singly and in combination was studied on photosynthetic and non-photosynthetic pigments, metabolites, enzyme activities, biomass accumulation and the net primary productivity of spinach (Spinacia oleracea L. var. All Green), a common leafy vegetable plant. Both the stresses individually and in combination resulted in the reduction of photosynthetic pigments, ascorbic acid, and catalase activity, whereas a reverse trend was observed for anthocyanin, flavonoids and proline content, lipid peroxidation and peroxidase activity. The interactive effects of both the stresses were, however, less than additive. Combined treatment of sUV-B + Cd was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spinaicia oleracea; Biomass; Cadmium; Enzymes; Nickel; Pigments; Ultraviolet-B.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000200007
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Effects of Zn and Cd accumulation on structural and physiological characteristics of barley plants Braz. J. Plant Physiol.
Sridhar,Balaji B. Maruthi; Han,Fengxiang X.; Diehl,Susan V.; Monts,David L.; Su,Yi.
The objectives of this study were to identify the structural changes caused by Zn and Cd accumulation in shoots and roots of barley (Hordeum vulgare) plants; and to correlate metal accumulation with anatomical, physiological and morphological changes. Potted plants were exposed to metal treatments of Zn and Cd for 19 and 16 d respectively. Leaves, stems and roots were harvested to identify structural changes and analyze metal accumulation. Barley effectively accumulated Zn (up to 11283 mg kg-1) and Cd (up to 584 mg kg-1) in the shoots. Microscopic structural changes, such as a decrease in intercellular spaces, breakdown of vascular bundles, and shrinkage of palisade and epidermal cells, occurred in leaves, stems and roots of plants treated with high...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anatomy; Cadmium; Hordeum vulgare; Microscopy; Phytoremediation; Zinc.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000100002
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