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	Provedor de dados BABT (1) BJMBR (1) Autor Cunha,V.M.N. (1) Einicker-Lamas,M. (1) Guelfi,Marieli (1) Maioli,Marcos Antonio (1) Mingatto,Fábio Erminio (1) Muzi-Filho,H. (1) Noel,F. (1) Quintas,L.E.M. (1) Rodriguez,J.B.R. (1) Tavares,Marco Aurélio (1) Valverde,R.H.F. (1) Mais... Palavra-chave Calcium homeostasis (2) Bioenergetics (1) Biotransformation (1) Ca2+/calmodulin-dependent kinase II (1) Calmodulin (1) Fipronil (1) Hepatotoxicity (1) Rat vas deferens (1) SERCA2 (1) Thapsigargin (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2015 (1) 2013 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Citotoxicity of Fipronil on Hepatocytes Isolated from Rat and Effects of Its Biotransformation BABT Guelfi,Marieli; Maioli,Marcos Antonio; Tavares,Marco Aurélio; Mingatto,Fábio Erminio. ABSTRACT The aim of this study was to characterize the mechanism of toxicity of fipronil on hepatocytes isolated from the rat and the effect of its biotransformation on the toxicological potential. The toxicity of fipronil was assessed by monitoring the oxygen consumption and mitochondrial membrane potential, intracellular ATP concentration, Ca2+ homeostasis and cell viability. The cell viability was evaluated by trypan blue exclusion in hepatocytes that were isolated from the normal rats and by the release of the enzymes alanine transaminase and aspartate transaminase in hepatocytes that were isolated from the normal rats or proadifen-pretreated rats. Fipronil reduced mitochondrial respiration in the cells that were energized with glutamate plus malate in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Fipronil; Hepatotoxicity; Bioenergetics; Biotransformation; Calcium homeostasis. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000600843 Rat vas deferens SERCA2 is modulated by Ca2+/calmodulin protein kinase II-mediated phosphorylation BJMBR Rodriguez,J.B.R.; Muzi-Filho,H.; Valverde,R.H.F.; Quintas,L.E.M.; Noel,F.; Einicker-Lamas,M.; Cunha,V.M.N.. Ca2+ pumps are important players in smooth muscle contraction. Nevertheless, little information is available about these pumps in the vas deferens. We have determined which subtype of sarco(endo)plasmic reticulum Ca2+-ATPase isoform (SERCA) is expressed in rat vas deferens (RVD) and its modulation by calmodulin (CaM)-dependent mechanisms. The thapsigargin-sensitive Ca2+-ATPase from a membrane fraction containing the highest SERCA levels in the RVD homogenate has the same molecular mass (∼115 kDa) as that of SERCA2 from the rat cerebellum. It has a very high affinity for Ca2+ (Ca0.5 = 780 nM) and a low sensitivity to vanadate (IC50 = 41 µM). These facts indicate that SERCA2 is present in the RVD. Immunoblotting for CaM and Ca2+/calmodulin-dependent protein... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ca2+/calmodulin-dependent kinase II; Calcium homeostasis; Calmodulin; Rat vas deferens; SERCA2; Thapsigargin. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000300227 Registros recuperados: 2 Primeira ... 1 ... Última

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