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| Registros recuperados: 35 | |
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| Faucet, Jerome; Maurice, Manuelle; Gagnaire, Beatrice; Renault, Tristan; Burgeot, Thierry. |
| As the marine mussel Mytilus edulis is commonly used as a sentinel species, it would be useful to develop a primary culture of the target organs most often in contact with the marine environment. This study reports an improved method for dissociating the digestive gland and gills of M. edulis and considers the effect of mussel storage on cell viability and functionality before culture initiation. Viability and enzymatic activities such as those of esterase and peroxidase were monitored by flow cytometry, a sensitive, objective technique allowing large volumes of cells to be counted within a short time. A primary culture of digestive gland showed more than 75% viability after 72 h. Mussels were maintained in an aquarium containing clean, oxygenated seawater... |
| Tipo: Text |
Palavras-chave: Mytilus edulis; Gill; Flow cytometry; Digestive gland; Cell culture. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-936.pdf |
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| Boher, S; Piclet, Guy; Beril-stien, S; Masson, Daniel; Delonce, R; Biziagos, E; Schwartzbrod, L. |
| Shellfish consumption is clearly associated with the transmission of enteric viruses. The channel of transmission of enteric viruses to human beings could be decreased particularly if the systems of depuration of shellfish were optimized, standardized and if their viral efficiency was controlled. The purpose of this work was to study the contamination of oysters and to assess the efficiency of several depuration processes. Our study have demonstrated that in some cases, 50% of oysters samples contain enteric viruses or H.A.V. antigen and the quantities of viruses range from 17 to 246 FF for the rotaviruses and from 124 to 200 for the enteroviruses per 100 g of tissue. According to our results, the exclusive use of a bacteriological criterium to determine... |
| Tipo: Text |
Palavras-chave: Rotavirus; Hepatitis A virus; Enterovirus; Crassostrea gigas; Bivalvia; Seafood; Bioassays; Cell culture; Viruses; Self purification; Viral diseases; Shellfish. |
| Ano: 1992 |
URL: http://archimer.ifremer.fr/doc/1992/acte-1615.pdf |
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| Delsert, Claude; Cancela Da Fonseca, Leonor. |
| The main objective of this project was to develop the tools for the in-depth understanding of mollusc biology and pathology. Such a goal has been reached in other biological systems through the development of molecular biology and cell culture. Indeed, development of a mollusc cell culture system is an essential step to approach many of the problems related to mollusc pathology, in particular to use molecular biology tools to study viral infection of these organisms. No continous cell line is available from bivalves and while cell cultures have been obtained from these organisms they are difficult to develop and labor intensive, survival cultures being maintained only for a few weeks. Our goal has been to study in detail many of the parameters involved in... |
| Tipo: Text |
Palavras-chave: Pathology; Mussel-specific expression vectors; Cell culture; Mytilus galloprovincialis; Gene; Genetic marker. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00044/15540/12917.pdf |
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| Dubois, Eric; Merle, Ghislaine; Roquier, Catherine; Trompette, Aurélien; Le Guyader, Soizick; Cruciere, Catherine; Chomel, Jean-jacques. |
| Oysters harvested in western France, from five sites associated with outbreaks of food-borne norovirus gastroenteritis between February 2000 and March 2001, were assayed for enterovirus RNA by reverse transcriptase-heminested polymerase chain reaction (RT-heminested PCR). Forty percent (21/52) of shellfish samples (pool of seven oysters) were contaminated by enteroviruses. Infectious coxsackieviruses serotype A21 were isolated from three of these positive samples. Amplicons corresponding to 65 base sequences in the 5' untranslated region of the enteroviral genome were analyzed by direct sequencing. Interpretable results were obtained from 18 amplicons, but mixtures of sequences confused the results from 3 samples. Sequences isolated from samples from the... |
| Tipo: Text |
Palavras-chave: Sequence analyze; RT PCR; Cell culture; Shellfish; Enterovirus. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/2004/publication-763.pdf |
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| Tamieti,B. P.; Damatta,R. A.; Cogo,J. C.; Da Silva,N. S.; Mittmann,J.; Pacheco-Soares,C.. |
| Snake venoms are toxic to a variety of cell types. However, the intracellular damages and the cell death fate induced by venom are unclear. In the present work, the action of the South American rattlesnake Crotalus durissus terrificus venom on CHO-K1 cell line was analyzed. The cells CHO-K1 were incubated with C. d. terrificus venom (10, 50 and 100g/ml) for 1 and 24 hours, and structural alterations of actin filaments, endoplasmic reticulum and nucleus were assessed using specific fluorescent probes and agarose gel electrophoresis for DNA fragmentation. Significant structural changes were observed in all analyzed structures. DNA fragmentation was detected suggesting that, at the concentrations used, the venom induced apoptosis. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cell culture; Actin filaments; Nucleus; Endoplasmic reticulum; Crotalus; Rattlesnake venom; Apoptosis. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000100004 |
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| Sihler,William; Souza,Marlinda Lobo de; Valicente,Fernando Hercos; Falcão,Rosana; Sanches,Marcio Martinello. |
| Abstract: The objective of this work was to evaluate the response of an in vitro host range to Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV), a pathogenic virus to the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae), for the further development of a biopesticide based on cell culture systems. The cell lines from Bombyx mori (BM-5), Lymantria dispar (IPLB-LD-625Y), Trichoplusia ni (BTI-Tn-5B1-4), Anticarsia gemmatalis (UFL-AG-286), and S. frugiperda (IPLB-SF-21AE and Sf9) were tested for their susceptibility to a highly-virulent Brazilian isolate of SfMNPV. The cytopathic effects induced by the virus, the production of viral particles, and the synthesis of viral polypeptides were examined and compared. Both S. frugiperda cell... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Baculovirus; Biological control; Cell culture; Fall armyworm; In vitro production; SfMNPV. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000100001 |
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| Rocha,A.R.; Leite,Y.K.C.; Silva,A.S.; Conde Júnior,A.M.; Costa,C.R.M.; Silva,G.C.; Bezerra,D.O.; Cavalcante,M.M.A.S.; Feitosa,M.L.T.; Argôlo Neto,N.M.; Serakides,R.; Carvalho,M.A.M.. |
| ABSTRACT There is a growing interest in the study of unspecialized mesenchymal stem cells, for there are still some discussions about their in vitro behavior. Regenerative medicine is a science undergoing improvement which develops treatments as cell therapy using somatic stem cells. In several studies, adipose tissue is presented as a source of multipotent adult cells that has several advantages over other tissue sources. This study aimed to characterize and evaluate the tagging of mesenchymal stem cells from the agoutis adipose tissue (Dasyprocta prymonolopha), with fluorescent intracytoplasmic nanocrystals. Fibroblast cells were observed, plastic adherent, with extended self-renewal, ability to form colonies, multipotency by differentiation into three... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cell culture; Mesenchymal; Stem cells; Adipose tissue; Cell differentiation; Fluorescent nanocrystals. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000501571 |
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| Salvarani,F.M.; Lobato,Z.I.P.; Assis,R.A.; Lima,C.G.R.D.; Silva,R.O.S.; Pires,P.S.; Lobato,F.C.F.. |
| Aiming to investigate in vitro alternatives, a test for neutralizing antibody detection using cell culture was developed. This test was more sensitive than previous animal models, allowing for detection of substantially lower alpha toxin and anti-alpha toxin titers. Titers observed during in vivo and in vitro seroneutralization had a correlation of 99.12%, indicating that cell culture is a viable alternative in the evaluation of vaccine potency, screening of vaccinal seeds, and Clostridium septicum alpha toxin titration. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Seroneutralization; Mouse; Guinea pig; Cell culture; Vaccine. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000400002 |
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| Lonien,S.C.H.; Bolognini,A.M.; Linhares,R.E.C.; Nozawa,C.M.. |
| A citopatologia in vitro de uma cepa de rotavírus porcino adaptado em cultura de células foi comparada à estirpe-protótipo símia (SA-11). O efeito citopático (ECP) produzido pelos vírus foi semelhante embora a estirpe porcina tivesse apresentado algumas alterações diferentes, como o acentuado estreitamento do citoplasma, com grande perda do volume citoplasmático. O vírus porcino apresentou menor número de plaques de ECP porém com diâmetro maior em relação ao vírus símio, demonstrando maior capacidade de disseminação célula-célula, quase oito vezes mais, a julgar pelo diâmetro dos plaques de ECP. Os elementos do citoesqueleto das células infectadas revelaram uma reorganização semelhante para ambas as estirpes, não sendo possível observar nenhuma diferença,... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Rotavirus; Cytopathology; Cell culture; Plaque; Cytoskeleton. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352001000400002 |
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| Rüttler,M.E.; Yanzón,C.S.; Cuitiño,M.J.; Renna,N.F.; Pizarro,M.A.; Ortiz,A.M.. |
| Enteroaggregative Escherichia coli (EAEC) has been implicated in sporadic diarrhea in children and adults and has been identified as the cause of several outbreaks worldwide. The HEp-2 test remains the gold standard for identification of this pathotype. A 60-65 MDa plasmid encodes the aggregative adherence fimbriae (AAF/I and AAF/II), a transcriptional activator ( aggR gene), the enteroaggregative heat-stable enterotoxin EAST1 ( astA gene) and a cytotoxin (Pet). The standard assay for EAEC is performed only in research laboratories, because it is expensive, labor intensive and time-consuming. The Polymerase Chain Reaction (PCR) offers the possibility of rapid diagnosis. In the current study, a multiplex PCR assay which checks aggR and astA genes was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarrhea; Escherichia coli; Cell culture; PCR (Polymerase Chain Reaction). |
| Ano: 2006 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000200006 |
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| Sun,Xuerong; Liu,Xinguang; Zhang,Yuehong; Kuang,Xielan; Lv,Bo; Ge,Jian. |
| Mechanical pressure plays an important role in many physiological and pathological processes. Mimicking the mechanical pressure present in vitro is necessary for related research, but usually requires expensive and complicated equipment. In this study we created a simple pressure culture system based on the transwell culture system. By cutting off the top rim of the transwell insert, the cells were compressed between the insert membrane and the well floor. The new pressure culture system was proven effective in that it induced cell morphological change, integrin β1 upregulation, actin polymerization and growth change in rat retinal ganglion cells, human nasopharyngeal carcinoma cells and mice embryonic fibroblasts. Though the pressure value is... |
| Tipo: Journal article |
Palavras-chave: Mechanical pressure; Cell culture; Transwell culture system; Cytoskeleton. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100007 |
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| Registros recuperados: 35 | |
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