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| Houllou-Kido,Laureen Michelle; Kido,Ederson Akio; Falco,Maria Cristina; Silva Filho,Márcio de Castro; Figueira,Antônio Vargas de Oliveira; Nogueira,Neusa de Lima; Rossi,Mônica Lanzoni; Tulmann Neto,Augusto. |
| A plant regeneration method with cell suspension cultures of banana, and the effect of biobalistic on regeneration potential are described in this report. Somatic embryos of banana were obtained from indirect embryogenesis of male inflorescence of banana cultivar Maçã (AAB group). Part of the calluses formed (40%) showed embryogenic characteristics (nonfriable, compact and yellow color). The cell suspension, originated from embryogenic calluses, contained clusters of small tightly packed cells with dense cytoplasms, relatively large nuclei and very dense nucleoli. After four months of culture, somatic embryos started to regenerate. The maximum number of regenerated plants was observed between 45 and 60 days after embryo formation.In the first experiment,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Musa; Cell suspension; Callus; Somatic differentiation; Biobalistic. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005001100005 |
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| SEIJO,GUILLERMO. |
| Changes in cell water relations during precultures were followed in an attempt to understand the mechanism of cell hardening for cryopreservation by vitrification. Medium containing 0.4 M sucrose (psiw=-1.45 MPa) and containing 5 mg L-1 of ABA (MT psiw=-0.73 MPa and MS psiw=-0.48 MPa) were used to harden cell suspensions of orange and carrot. Preculture in these medium did not cause a significant decrease of cell viability, however, it improved the cell survival to PVS2 and liquid nitrogen expositions. When cells were inoculated into medium containing 0.4 M sucrose, the cell psiw decreased rapidly until reach the medium psiw , and turgor was also severely reduced or disappeared. Subsequently, cell psis began to decrease and after 48 h of treatment in low... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Preculture; Cell suspension; Sucrose; ABA; Cryopreservation. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312000000200007 |
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