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| Marques,Daniela A. Viana; Santos-Ebinuma,Valéria de Carvalho; Oliveira,Patrícia Maria Sobral de; Lima,Gláucia Manoella de Souza; Araújo,Janete M.; Lima-Filho,José L.; Converti,Attilio; Pessoa-Júnior,Adalberto; Porto,Ana L.F.. |
| The selection of new microorganisms able to produce antimicrobial compounds is hoped for to reduce their production costs and the side effects caused by synthetic drugs. Clavulanic acid is a β-lactam antibiotic produced by submerged culture, which is widely used in medicine as a powerful inhibitor of β-lactamases, enzymes produced by bacteria resistant to antibiotics such penicillin and cephalosporin. The purpose of this work was to select the best clavulanic acid producer among strains of Streptomyces belonging to the Microorganism Collection of the Department of Antibiotics of the Federal University of Pernambuco (DAUFPE). Initially, the strains were studied for their capacity to inhibit the action of β-lactamases produced by Klebsiella aerogenes ATCC... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clavulanic acid; Β-lactamases inhibitor; Streptomyces screening; Submerged culture; Growth kinetics. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300022 |
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| Marques,Daniela A. Viana; Cunha,Márcia N. Carneiro; Araújo,Janete M.; Lima-Filho,José L.; Converti,Attilio; Pessoa-Jr,Adalberto; Porto,Ana L. Figueiredo. |
| Clavulanic acid is a β-lactam antibiotic which has a potent β-lactamase inhibiting activity. In order to optimize its production by the new isolate Streptomyces DAUFPE 3060, the influence of two independent variables, temperature and soybean flour concentration, on clavulanic acid and biomass concentrations was investigated in 250 mL-Erlenmeyers according to a 2² central composite design. To this purpose, temperature and soybean flour (SF) concentration were varied in the ranges 26-34°C and 10-50 g/L, respectively, and the results evaluated utilizing the Response Surface Methodology. The experimental maximum production of clavulanic acid (629 mg/L) was obtained at 32°C and 40 g/L SF after 48 h, while the maximum biomass concentration (3.9 g/L) at 30°C and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clavulanic acid; Response surface methodology; Production; Optimization; Streptomyces sp. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200030 |
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| Rodrigues,Kaio César da Silva; Souza,Arianne Tairyne de; Badino,Alberto Colli; Pedrolli,Danielle Biscaro; Cerri,Marcel Otavio. |
| ABSTRACT Clavulanic acid is a β-lactam compound with potent inhibitory activity against β-lactamases. Studies have shown that certain amino acids play essential roles in CA biosynthesis. However, quantitative evaluations of the effects of these amino acids are still needed in order to improve CA production. Here, we report a study of the nutritional requirements of Streptomyces clavuligerus for CA production. Firstly, the influence of the primary nitrogen source and the salts composition was investigated. Subsequently, soybean protein isolate was supplemented with arginine (0.0–3.20 g L-1), threonine (0.0–1.44 g L-1), ornithine (0.0–4.08 g L-1), and glutamate (0.0–8.16 g L-1), according to a two-level central composite rotatable design. A medium containing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clavulanic acid; Streptomyces clavuligerus; Ferrous sulfate; Amino acids supplementation. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400832 |
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