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	Provedor de dados Ciênc. Tecnol. Aliment. (1) BJM (1) Autor Baseggio,Andressa Mara (1) Boujelben,Ines (1) Cattani,Cristhiane Stecanella de Oliveira (1) Cirolini,Andréia (1) Gdoura,Radhouane (1) Hammami,Adnane (1) Miotto,Marília (1) Ramos,Roberta Juliano (1) Vieira,Cleide Rosana Werneck (1) Mais... Palavra-chave Conventional methods (2) Alternative methods (1) Broad-range PCR (1) Infective endocarditis (1) Microbial contamination (1) Milk (1) Negative culture (1) Phenotypic investigations (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2018 (1) 2013 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A broad-range PCR technique for the diagnosis of infective endocarditis BJM Boujelben,Ines; Gdoura,Radhouane; Hammami,Adnane. Abstract Infective endocarditis (IE) remains a severe and potentially fatal disease demanding sophisticated diagnostic strategies for detection of the causative microorganisms. The aim of the present study was to develop a broad-range 16S ribosomal RNA gene polymerase chain reaction in the routine diagnostic of IE for the early diagnosis of fatal disease. A broad-range PCR technique was selected and evaluated in terms of its efficiency in the diagnosis of endocarditis using 19 heart valves from patients undergoing cardiovascular surgeries at the Habib Bourguiba Hospital of Sfax, Tunisia, on the grounds of suspected IE. The results demonstrated the efficiency of this technique particularly in cases involving a limited number of bacteria since it helped to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Broad-range PCR; Infective endocarditis; Conventional methods; Negative culture; Phenotypic investigations. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000300534 Evaluation of the PetrifilmTM and TEMPO® systems and the conventional method for counting microorganisms in pasteurized milk Ciênc. Tecnol. Aliment. Cirolini,Andréia; Baseggio,Andressa Mara; Miotto,Marília; Ramos,Roberta Juliano; Cattani,Cristhiane Stecanella de Oliveira; Vieira,Cleide Rosana Werneck. New microbiological methods have been developed and commercialized, but their performance must be guaranteed. The aim of the present study was to evaluate the PetrifilmTM and TEMPO® systems compared to the conventional method for counting microorganisms in pasteurized milk. A total of 141 samples of pasteurized milk were analyzed by counting mesophilic aerobic, Coliforms at 35 ºC, Coliforms at 45 ºC, and Escherichia coli microorganisms. High correlation was found between the methods for counting Coliforms at 35 ºC, but low correlation was found for counting mesophilic aerobic, Coliforms at 45 ºC, and Escherichia coli. No significant statistical difference was found among the three methods for counting Coliforms at 35 ºC; however, the mean counts of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Alternative methods; Milk; Microbial contamination; Conventional methods. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000400026 Registros recuperados: 2 Primeira ... 1 ... Última

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