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Mello,S.L.V.; Henry,M.; Souza,M.C.; Oliveira,S.M.P.. |
The aim of this study was to evaluate the longevity of donkey sperm comparing the rich seminal fraction and the whole semen in two extenders, Kenney and modified Baken extenders. Semen of five donkeys were collected through an open-end artificial vagina once a week for five consecutive weeks. The two first jets (rich fraction) of semen were collected separately from the rest of the ejaculate. Whole semen samples were obtained mixing proportionally part of the rich with part of the poor seminal fractions. Seminal samples were immediately diluted 1:1 in each extender and maintained at room temperature during sperm concentration analysis. Samples were further diluted to rich 50×10(6) sperm per ml, cooled in a refrigerator at the initial rate of -0.6° C/min... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Donkey; Cooled semen; Sperm longevity. |
Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000400015 |
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Rossi,R.; Gonczarowska,A.T.; Palhares,M.S.; Boeta,A.M.; Resende,Y.F.; Ribeiro,E.A.; Silva Filho,J.M.. |
Fertility obtained by cross-breeding mares (Equus caballus) with jackasses (Equus asinus) was evaluated. Two extenders, containing skim milk-glucose or egg yolk-glycine were used to study the fertility of mares inseminated with diluted jackass semen (T1 and T2) or diluted and cooled semen at 5°C for 12 hours (T3 and T4). A total of 272 cycles of 208 mares of undefined breeds were evaluated, being uniformly distributed between groups. The cycles were controlled by transrectal palpation and teasing, and mares were inseminated every Tuesday, Thursday and Saturday (three times/week), from the detection of a follicle with 3.0 to 3.5cm diameter in one of the ovaries until ovulation. Pregnancy was detected using transrectal palpation, teasing and ultrasound exams... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fertility; Pregnancy loss; Intra and interspecific cross-breeding; Cooled semen. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000100057 |
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Carvalho,L.E.; Silva Filho,J.M.; Palhares,M.S.; Sales,A.L.R.; Gonczarowska,A.T.; Oliveira,H.N.; Resende,M.C.; Neves,M.G.; Madison,R.J.. |
ABSTRACT The aim of this study was to evaluate the effect of two different extenders (Skimmed Milk Glucose - SMG or Lactose - Egg Yolk - LEY) on physical characteristics and fertility of fractionated donkey semen cooled at 5°C. For this, four Pêga donkeys were used as semen donors. The sperm rich fraction of the ejaculate was diluted preparing insemination doses containing 400 x 106 motile spermatozoa in a volume of 22 mL, cooled to 5°C and stored up to 48 hours in a container proposed by Palhares (1997). Sperm motility and vigor were assessed in fresh semen, after first semen dilution, before insemination, at 24 and 48 hours after storage. For the fertility evaluation, 44 mares were inseminated with semen stored for a period between 12 and 24 hours. The... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Donkey; Fractionated collection; Cooled semen; Fertility. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000100029 |
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