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Variations in the sensitivity of different primers for detecting Wolbachia in Anastrepha (diptera: tephritidae) BJM
Marcon,Helena Sanches; Coscrato,Virgínia Elias; Selivon,Denise; Perondini,André Luiz Paranhos; Marino,Celso Luis.
Wolbachia are endosymbiont bacteria of the family Rickettsiacea that are widespread in invertebrates and occur between 20% and 60% of Neotropical insects. These bacteria are responsible for reproductive phenomena such as cytoplasmic incompatibility, male killing, feminization and parthenogenesis. Supergroups A and B of Wolbachia are common in insects and can be identified using primers for 16S rDNA, ftsZ and wsp; these primers vary in their ability to detect Wolbachia. The ftsZ primer was the first primer used to detect Wolbachia in Anastrepha fruit flies. The primers for 16S rDNA, ftsZ and wsp and the corresponding PCR conditions have been optimized to study the distribution of Wolbachia and their effect on the biology of Anastrepha in Brazil. In this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anastrepha; Cross reaction; FtsZ; Wsp.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200046
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