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Hemolytic activity of venom from crown-of-thorns starfish Acanthaster planci spines J. Venom. Anim. Toxins incl. Trop. Dis.
Lee,Chi-Chiu; Tsai,Wann-Sheng; Hsieh,Hernyi Justin; Hwang,Deng-Fwu.
Background : The crown-of-thorns starfish Acanthaster planci is a venomous species from Taiwan whose venom provokes strong hemolytic activity. To understand the hemolytic properties of A. planci venom, samples were collected from A. planci spines in the Penghu Islands, dialyzed with distilled water, and lyophilized into A. planci spine venom (ASV) powder. Results : Both crude venom and ASV cause 50% hemolysis at a concentration of 20 μg/mL. The highest hemolytic activity of ASV was measured at pH 7.0-7.4; ASV-dependent hemolysis was sharply reduced when the pH was lower than 3 or greater than 8. There was almost no hemolytic activity when the Cu2+ concentration was increased to 10 mM. Furthermore, incubation at 100°C for 30 to 60 minutes sharply decreased...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Crown-of-thorns starfish; Spine; Venoms; Hemolysis.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992013000100312
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