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| Registros recuperados: 26 | |
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| Pérez Ramírez, Jacqueline. |
| En esta investigación, se evaluó el efecto de las diluciones del agua de coco y la adición de biotina sobre el crecimiento de Saccharomyces cerevisiae. En este sentido, la dilución 1:1 adicionada con biotina (µ= 0.21 h-1) y biomasa (3.32 g/L) fue suficiente para dar los requerimientos nutricionales a la cepa estudiada, en comparación con el agua de coco 100% (µ= 0.13 h-1) y biomasa (3.28 g/L) y un medio cultivo sintético (µ= 0.12 h-1) con biomasa de 2.86 g/L. Los nutrientes disponibles en diluciones 1:2 (µ= 0.18 h-1) y biomasa (2.25 g/L), podrían ser considerables para estudios posteriores; no así, las diluciones 1:5 y 1:10 que parecen inadecuadas para el óptimo crecimiento de S. cerevisiae. Se observó el efecto positivo de la biotina sobre el crecimiento... |
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Palavras-chave: Cepa; Medio de cultivo; Biomasa; Strain; Culture medium; Biomass production; Maestría Tecnológica; Agroindustria. |
| Ano: 2011 |
URL: http://hdl.handle.net/10521/643 |
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| Pontes,Nadson C; Fujinawa,Miriam F; Oliveira,José R. |
| ABSTRACT The efficacy of five selective media described in the literature for the detection of Ralstonia solanacearum in soil was evaluated using isolates from different regions of Brazil. The selective media were evaluated regarding their sensitivity, target-pathogen repression index, capacity for suppressing contaminating microorganisms and recovery rate of the pathogen in soil. No bacterial growth was observed at the concentrations tested for FSM and SM-1 media. Some isolates grew on the PCCG medium with the addition of antimicrobial agents, but only at concentrations of 106 cfu/mL. For the SM and SMSA-E media, adequate sensitivity for the detection of R. solanacearum in soil was observed, enabling detection at a concentration of approximately 103... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial wilt; Culture medium; Diagnosis; SMSA-E.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362017000100041 |
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| Bhatt,Arvind; Stanly,Christine; Keng,Chan Lai. |
| The influence of cytokinin, N6-benzyladenine, on shoot proliferation of five Alocasia species (A. amazonica, A. cuprea, A. robusta, A. longiloba and A. chaii) was investigated. In vitro propagation of these species was established using shoot tip explants. Murashige & Skoog (MS) medium supplemented with different concentrations of BA (N6-benzyladenine) ranging from 0, 2, 5, 10 mg/L was then used to establish the optimum medium for shoot proliferation for all the species. MS medium supplemented with 2.0 mg/L BA was optimum for the shoot proliferation. All the tested species showed varying results for shoot number and shoot height. A comparison between agar-gelled medium and shake flask system using liquid medium was carried out to evaluate the shoot... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alocasia amazonica; A. cuprea; A. robusta; A. longiloba; A. chaii; Culture medium; Acclimatization. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000200006 |
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| SANTOS, H. M. G. dos; CARVALHO, A. C. P. P. de; SCHNEIDER, F.; ROSSETTI, A. G.; CAMPOS, A. S.. |
| O ágar é o gelificante mais utilizado no preparo de meios de cultivo semissólidos na micropropagação vegetal. Porém, por ser um dos componentes de maior custo, agentes gelificantes alternativos vêm sendo prospectados, razão do objetivo deste trabalho ter sido avaliar o desempenho de mudas micropropagadas de ananeira, cv. Prata Cataria, na fase de alongamento e enraizamento, em meio de cultivo MS contendo diferentes gelificantes alternativos, tais como sagu, fécula de mandioca e amido de milho, produtos encontrados em supermercados. Agar is the most widely used gelling agent in the preparation of semi-solid culture media in plant micropropagation. However, because it is one of the most costly components, alternative gelling agents have been prospecting. The... |
| Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) |
Palavras-chave: Agentes gelificantes; Meio de cultivo; Bananeira; Fécula de mandioca; Amido de milho; Maize starch; Culture medium; Musa sp; Micropropagação; Cassava starch; Gelling agents. |
| Ano: 2020 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1125505 |
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| Dantas,Adriana Cibele de Mesquita; Boneti,José Itamar; Nodari,Rubens Onofre; Guerra,Miguel Pedro. |
| The objetive of this work was to rescue immature embryos of apple rootstocks Malus prunifolia (Marubakaido) and Malus pumila (M9) after 40-60 days of pollination and to put them into MS culture media supplemented with agar (6 g L-1) and casein hydrolysate (500 mg L-1). Embryos originated from interspecific crosses and open pollination showed differences in the in vitro responses, depending on the female parent, the developmental stage of the embryo, and the culture medium composition. Embryos of the M. pumila rootstock, rescued within 40 days after pollination and put in culture medium supplemented with indolacetic acid (IAA), gibberellic acid (GA3), kinetin and maltose, resulted in a normal development of plantlets. However, embryos originating from... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Malus; Marubakaido; M9; Culture medium; Carbon source. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000600011 |
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| URSI,SUZANA; PLASTINO,ESTELA MARIA. |
| Gracilaria sp. é uma das principais macroalgas marinhas atualmente coletadas para produção de ágar no Brasil. Este trabalho objetivou comparar o crescimento in vitro de diferentes estádios reprodutivos tanto de linhagens selvagens (vermelhas), quanto de uma variante cromática (verde clara) de Gracilaria sp., em dois meios de cultura: Von Stosch e Provasoli. Seis linhagens foram selecionadas: gametófitos femininos (F) e masculinos (M), vermelhos (vm) e verde claros (vc); tetrasporófitos vermelhos, porém derivados de cruzamentos distintos, Fvm x Mvm e Fvc x Mvm. As taxas de crescimento avaliadas por 28 dias, foram calculadas a partir de medidas de massa fresca (TCm) e comprimento (TCc). A variante cromática apresentou TCm e TCc inferiores às verificadas para... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gracilaria; Growth; Color strain; Reproductive stage; Culture medium. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000500014 |
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| Guollo,Karina; Wagner Junior,Américo; Kosera Neto,Carlos; Radaelli,Juliana Cristina; Keller,Welida Mayara Tomazoni. |
| Abstract Sete-capote tree, a neglected species, has great ecological, cultural and potential economic importance, however, studies for this and other native species of the Myrtaceae family are still scarce. The objective of this study was to elucidate aspects of pollen germination and viability of this specie. For the experiment, pollen from flowers in pre and post anthesis was used, which was dehydrated in a chamber containing silica, for different periods. For germination tests, different concentrations of sucrose, boric acid and calcium nitrate were used. After obtaining germination results above 80%, the pollen grains were stored in refrigerator (5 °C), freezer (-17 °C), liquid nitrogen (-147 °C) and natural environment (± 25 °C), evaluating monthly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Native fruit tree; Culture medium; Myrtaceae; Sete-capote tree. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452021000100803 |
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| Santos, Ana Claudia Schllemer dos; Castro, Juliana Dias de; Bressan, Daiane; Júnior, Américo Wagner; Guollo, Karina. |
| The aim of this work was to determine the ideal stage of pollen collection and the best concentrations of sucrose and boric acid in the culture medium for the in vitro germination of inga fruit tree pollen. For this, the work was carried out in two stages, one using concentrations of sucrose in the culture medium (0, 5, 10, 20 and 30%) - experiment 1. Another one using the best sucrose concentration, with concentrations (0, 100, 200 and 300 mg L-1) - experiment 2. In the two experiments, two flowering phases were use: pre-anthesis and post-anthesis. For both experiments, the culture medium contained 1% agar to solidify the medium and all treatments remained in BOD for 24 h at a temperature controlled at 25°C. For the experiment 1 with sucrose, DIC was use... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Culture medium; Fabaceae; Native fruittree; Inga edulis Mart.; Pollen tube. |
| Ano: 2018 |
URL: http://e-revista.unioeste.br/index.php/scientiaagraria/article/view/19619 |
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| Pérez-Martínez,Belkys Adriana; Castañeda-Garzón,Sandra Liliana. |
| Abstract This research aimed to evaluate techniques for in vitro multiplication and rooting and ex vitro adaptation of wild blackberry plants (Rubus bogotensis Benth. and Rubus macrocarpus Kunth). In in vitro phases, the influence of culture media was evaluated based on Murashige & Skoog (MS) composition, with or without a 50% reduction in salts, vitamins, and myo-inositol and supplemented or not with benzylaminopurine (BAP), indole acetic acid (IAA), L-cysteine, agar, peat, perlite, and vermiculite. The ex vitro phase was based on a soil, rice husks, peat, and perlite substrate management. After two months of evaluation of the in vitro phases, it was determined that the MS medium at 50% salts, vitamins, and myo-inositol and supplemented with sucrose... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Blackberry; Culture medium; High Andean forest; Plant growth regulator; Wild species. |
| Ano: 2017 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122017000100102 |
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| Martendal, Cíntia de Oliveira; Universidade Estadual de Maringá; Bernardino, Murilo Martins; Instituto Federal Goiano; Pereira, Flávia Dionísio; Cooperativa Agroindustrial dos Produtores Rurais do Sudoeste Goiano Ltda.; Silva, Fabiano Guimarães; Instituto Federal Goiano; de Menezes, Carlos César Evangelista; Cooperativa Agroindustrial dos Produtores Rurais do Sudoeste Goiano Ltda.; Monteiro Hara, Alessandra Cristina Boffino de Almeida; Instituto Federal Goiano. |
| The objective of this study is to establish an in vitro germination and cultivation protocol for murici (Byrsonima cydoniifolia A. Juss.) using zygotic embryos. Therefore, three assays were performed: in assay I, embryo asepsis was tested at exposure times of 5, 10, 15, 20, 25, and 30 minutes in 2.5% sodium hypochlorite, with or without immersion in 70% alcohol; in assay II, MS (MURASHIGE; SKOOG, 1962) e WPM (LLOYD; McCOWN, 1980) culture media were tested at salt concentrations of 25, 50, and 100%, with or without the addition of sucrose, to germinate the buds; in assay III, seedling growth was evaluated in MS and WPM culture media at salt concentrations of 25, 50 and 100%. Sodium hypochlorite (2.5%) with or without 70% alcohol was used to avoid... |
| Tipo: Pesquisa Empírica de Campo |
Palavras-chave: 5.01.03.04-0 tissue culture; Asepsis; Culture medium; Cerrado fruit Fitotecnia. |
| Ano: 2013 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15402 |
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| Brondani, Gilvano Ebling; Universidade Federal do Paraná; Dutra, Leonardo Ferreira; Empresa Brasileira de Pesquisa Agropecuária; Wendling, Ivar; Empresa Brasileira de Pesquisa Agropecuária; Grossi, Fernando; Universidade Federal do Paraná; Hansel, Fabricio Augusto; Empresa Brasileira de Pesquisa Agropecuária; Araujo, Marla Alessandra; Universidade Federal de Lavras. |
| This study was designed to micropropagate E. benthamii x E. dunnii, by testing chlorine concentrations for explant asepsis, the optimal concentrations of benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) for bud proliferation, and the ratio between BAP and gibberellic acid (GA3) in two nutrient media for shoot elongation. Nodal segments from H12, H19 and H20 clones were disinfected with 0.5, 1.0, 1.5 and 2.0% (v v-1) of chlorine. Explants were grown on ½MS medium supplemented with BAP (0, 0.25, 0.50, 0.75 and 1.00 mg L-1) and NAA (0, 0.025, 0.050, 0.075 and 0.100 mg L-1) for bud production. They were elongated on MS and ½MS media supplemented with BAP (0, 0.05 and 0.10 mg L-1) and GA3 (0, 0.1, 0.2 and 0.3 mg L-1). The 0.50 mg L-1 BAP and 0.050 mg... |
| Tipo: Pesquisa |
Palavras-chave: 5.01.03.04-0 in vitro establishment; Culture medium; Cloning; BAP; NAA; GA3 Produção de Mudas in vitro establishment; Culture medium; Cloning; BAP; NAA; GA3. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/8317 |
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| Soares, Joyce Dória Rodrigues; Universidade Federal de Lavras; Pasqual, Moacir; Universidade Federal de Lavras; Rodrigues, Filipe Almendagna; Universidade Federal de Lavras; Villa, Fabiola; Universidade Federal de Lavras; Araujo, Aparecida Gomes de; Universidade Federal de Lavras. |
| O objetivo deste trabalho foi determinar a fonte de silício que proporcione melhor crescimento em plântulas de Cattleya loddigesii. Plântulas oriundas de sementes germinadas in vitro com aproximadamente 1,0 cm de comprimento foram inoculadas em tubos de ensaio contendo 15 mL de meio de cultura Knudson C modificado, acrescido de silicato de sódio (0, 5, 10 e 20 mg L-1) e silicato de potássio (0, 5, 10 e 20 mL L-1), em todas as combinações possíveis. O meio de cultura teve seu pH ajustado para 5,8 ± 0,1 e solidificado com 5 g L-1 de ágar antes do processo de autoclavagem a 121°C e 1 atm por 20 min. O cultivo foi mantido em sala de crescimento com irradiância de 35 µmol m-2 s-1, temperatura de 25 ± 1°C e... |
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Palavras-chave: 5.01.03.00-8 Cattleya loddigesii; Meio de cultura; Orchidaceae; Cultura de tecidos Fitotecnia/Biotecnologia Cattleya loddigesii; Culture medium; Orchidaceae; Tissue culture. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6281 |
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| Zanandrea,Ilisandra; Bacarin,Marcos Antonio; Braga,Eugenia Jacira Bolacel; Bianchi,Valmor João; Peters,José Antonio. |
| The aim of the present study was to evaluate the growth and development of apple rootstock shoots submitted to different photon flux densities (7, 14, 21 and 60 µmol m-2 s-1) and three culture media. The best photon flux for maximizing growth, number of shoots and leaves was 14 µmol m-2 s-1, which also resulted in the highest values of chloroplastic pigments. On the other hand, the highest photon flux (60 µmol m-2 s-1) decreased significantly these parameters. Medium with reduced sucrose concentration did not affect the analyzed parameters, enabling an efficient multiplication of the cultivar with only 1% of sucrose. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mallus; Apple; Sucrose; Photon flux density; Culture medium. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000500005 |
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| Santos,Silvia; Baruque-Ramos,Júlia; Tanizaki,Marta Massako; Lebrun,Ivo; Schenkman,Rocilda Perazzini Furtado. |
| Meningococcal disease is an important cause of death and morbidity throughout the world. Nearly 330,000 cases and 35,000 deaths occur yearly. Neisseria meningitidis, serogroup B strain N.44/89, is prevalent in Brazil. Its outer membrane vesicles (OMV) with iron regulated proteins (IRP) are released to the culture medium and are used as antigen for vaccine production. In order to have knowledge about the kinetic parameters, especially the final OMV concentration values, 20-h batch cultivations were carried out in Catlin medium with iron restriction. Process conditions comprised: 7 L bioreactor, 36ºC, 0.5 atm, overlay air flowrate of 1 L/min, agitation varying from 250 rpm to 850 rpm and dissolved oxygen control set at 10% of saturation condition. Biomass... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neisseria meningitidis; Outer membrane vesicles; Culture medium; Vaccine; Submerged batch cultivation. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400016 |
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| Chen,Yong-Hao; Li,Jun; Liu,Li; Liu,Hong-Zhi; Wang,Qiang. |
| A mutant designated NC2168, which was selected from wild-type Streptococcus equisimilis CVCC55116by ultraviolet ray combined with60Co-γ ray treatment and does not produce streptolysin, was employed to produce hyaluronic acid (HA). In order to increase the output of HA in a flask, the culture medium and conditions for NC2168 were optimized in this study. The influence of culture medium ingredients including carbon sources, nitrogen sources and metal ions on HA production was evaluated using factional factorial design. The mathematical model, which represented the effect of each medium component and their interaction on the yield of HA, was established by the quadratic rotary combination design and response surface method. The model estimated that, a maximal... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus equisimilis mutant; Hyaluronic acid; Optimization; Culture medium; Culture conditions. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400040 |
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| Cantarelli,Vlademir Vicente; Inamine,Everton; Brodt,Teresa Cristina Zenobin; Secchi,Carina; Pereira,Fabiana de Souza; Amaro,Maria Cristina; Batalha,Alice Aurora; Ligiero,Simone Dornellas. |
| Stringent quality control protocols must be used in order to guaranty that a particular medium is able to recover all sort of organism that may be present in clinical samples. Our aim was to evaluate an alternative protocol that would allow us to detect medium failure to yield quantitative growth of selected pathogens, and compare with the document M22-A2 from NCCLS. The detection limit of Haemophilus influenzae was significantly different depending on media source. We conclude that for some fastidious microorganisms, quantitative verification of the growth capacity of the culture medium is advised. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Quality control; Culture medium; NCCLS. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500003 |
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| Paz,Marcelo Fossa da; Baruque-Ramos,Júlia; Hiss,Haroldo; Vicentin,Márcio Alberto; Leal,Maria Betania Batista; Raw,Isaías. |
| Polysaccharide of N. meningitidis serogroup C constitutes the antigen for the vaccine against meningitis. The goal of this work was to compare three cultivation media for production of this polysaccharide: Frantz, modified Frantz medium (with replacement of glucose by glycerol), and Catlin 6 (a synthetic medium with glucose). The comparative criteria were based on the final polysaccharide concentrations and the yield coefficient cell/polysaccharide (Y P/X). The kinetic parameters: pH, substrate consumption and cell growth were also determined. For this purpose, 9 cultivation runs were carried out in a 80 L New Brunswick bioreactor, under the following conditions: 42 L of culture medium, temperature 35ºC, air flow 5 L/min, agitation frequency 120 rpm and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neisseria meningitidis; Culture medium; Vaccine; Polysaccharide; Batch cultivation. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100007 |
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| Registros recuperados: 26 | |
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