SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Gomes,Cristiano C.; Moreira,Leonardo M.; Santos,Vanessa J.S.V.; Ramos,Alfeu S.; Lyon,Juliana P.; Soares,Cristina P.; Santos,Fabio V..

The use of artificial implants provides a palliative or permanent solution for individuals who have lost some bodily function through disease, an accident or natural wear. This functional loss can be compensated for by the use of medical devices produced from special biomaterials. Titanium alloy (Ti-6Al-4V) is a well-established primary metallic biomaterial for orthopedic implants, but the toxicity of the chemical components of this alloy has become an issue of concern. In this work, we used the MTT assay and micronucleus assay to examine the cytotoxicity and genotoxicity, respectively, of an extract obtained from this alloy. The MTT assay indicated that the mitochondrial activity and cell viability of CHO-K1 cells were unaffected by exposure to the...

Profile of micronucleus frequencies and DNA damage in different species of fish in a eutrophic tropical lake

Palavras-chave: Biomaterial; Cytotoxicity; Micronucleus; Mutagenicity; Ti-6Al-4V.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100020

Grisolia,Cesar K.; Rivero,Carla L. G.; Starling,Fernando L. R. M.; Silva,Izabel C. R. da; Barbosa,Antonio C.; Dorea,Jose G..

Lake Paranoá is a tropical reservoir for the City of Brasilia, which became eutrophic due to inadequate sewage treatment associated with intensive population growth. At present, two wastewater treatment plants are capable of processing up to 95% of the domestic sewage, thereby successfully reducing eutrophization. We evaluated both genotoxic and cytotoxic parameters in several fish species ( Geophagus brasiliensis , Cichla temensis , Hoplias malabaricus , Astyanax bimaculatus lacustres , Oreochromis niloticus , Cyprinus carpio and Steindachnerina insculpita ) by using the micronucleus (MN) test, the comet assay and nuclear abnormality assessment in peripheral erythrocytes. The highest frequencies of MN were found in Cichla temensis and Hoplias...

Studies of micronuclei and other nuclear abnormalities in red blood cells of Colossoma macropomum exposed to methylmercury

Palavras-chave: Genotoxicity; Fish biomonitoring; Eutrophic lake; Micronucleus; Comet assay; Cytotoxicity.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100021

Rocha,Carlos Alberto Machado da; Cunha,Lorena Araújo da; Pinheiro,Raul Henrique da Silva; Bahia,Marcelo de Oliveira; Burbano,Rommel Mario Rodríguez.

The frequencies of micronuclei (MN) and morphological nuclear abnormalities (NA) in erythrocytes in the peripheral blood of tambaqui (Colossoma macropomum), treated with 2 mg.L-1 methylmercury (MeHg), were analyzed. Two groups (nine specimens in each) were exposed to MeHg for different periods (group A - 24 h; group B - 120 h). A third group served as negative control (group C, untreated; n = 9). Although, when compared to the control group there were no significant differences in MN frequency in the treated groups, for NA, the differences between the frequencies of group B (treated for 120 h) and the control group were extremely significant (p < 0.02), thus demonstrating the potentially adverse effects of MeHg on C. macropomum erythrocytes after...

LNO3 AND L3 Are Associated With Antiproliferative And Pro-Apoptotic Action In Hepatoma Cells

Palavras-chave: Cytotoxicity; Genotoxicity; Methylmercury; Micronuclei assay.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400024

Zanelatto,Leonardo Campos; Silva,Patrícia Benites Gonçalves da; Sartori,Daniele; Panis,Carolina; Lepri,Sandra; Fátima,Ângelo de; Mantovani,Mário Sérgio.

Abstract The identification of antitumoral substances is the focus of intense biomedical research. Two structural analogues of thalidomide, LNO3 and L3, are two synthetic compounds that might possess such antitumor properties. We evaluated the toxicological effects of these substances, including cytotoxicity, genotoxicity and induction of apoptosis in HTC cells. Additionally, the production of free radicals (nitric oxide and superoxide) was investigated, and the expression of caspases genes 3, 8, and 9 were determined by RT-qPCR. The compounds exhibited cytotoxic effects that resulted in inhibited cell proliferation. LNO3 showed to be more effective and toxic than L3 in all assays. LNO3 stimulated the release of NO and superoxide, which was accompanied by...

Protective effects of bark ethanolic extract from Spondias dulcis Forst F. against DNA damage induced by benzo[a]pyrene and cyclophosphamide

Palavras-chave: Thalidomide analogues; Antiproliferative activity; Cytotoxicity; Free radicals; Apoptosis.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200270

Araujo,Caroline de S.; Brito,Lorrane D.; Tarifa,Marina O.; Silva,Nayara J. Farah da; Rodrigues,Karoline S.; Cavalcante,Dalita G. S. M.; Gomes,Andressa S.; Zocoler,Marcos A.; Yoshihara,Eidi; Camparoto,Marjori L.; Job,Aldo E.; Kerche,Leandra E..

Abstract This study evaluated the genotoxicity, mutagenicity, antigenotoxicity, and antimutagenicity effects on biochemical parameters of oxidative stress of the Spondias dulcis bark ethanolic extract on mice. The extract was evaluated in the doses of 500, 1000, and 1500 mg/kg bw via gavage. To evaluate the protective effects of the extract, benzo[a]pyrene (B[a]P) and cyclophosphamide (CP) were chosen as DNA damage inducers. Genotoxicity and antigenotoxicity were evaluated by the comet assay. Cytotoxicity, mutagenicity, and antimutagenicity were evaluated by the micronucleus test in bone marrow and peripheral blood. The biochemical parameters of oxidative stress were evaluated by the quantification of catalase activity (CAT) and reduced glutathione (GSH)...

Mitodepressive and clastogenic effects of aqueous extracts of the lichens Myelochroa lindmanii and Canoparmelia texana (Lecanorales, Parmeliaceae) on meristematic cells in plant bioassays

Palavras-chave: Comet assay; Micronucleus test; Spondias dulcis; Cytotoxicity; Mutagenicity.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400643

Campos,José Marcello Salabert de; Davide,Lisete Chamma; Soares,Geraldo Luiz Gonçalves; Viccini,Lyderson Facio.

The cytotoxicity effect of aqueous extracts of the lichens species Myelochroa lindmanii and Canoparmelia texana (Lecanorales, Parmeliaceae) were evaluated using meristematic cells of lettuce (Lactuca sativa) and maize (Zea mays). The seasonal effect was also evaluated. Extracts of M. lindmanii and C. texana inhibited root growth and/or percentage germination, possibly due to alterations in the cell cycle. The M. lindmanii extract showed anti-mitotic effects and blocked the cell cycle in metaphase so that c-mitosis and cells with chromosome duplication were produced. The C. texana extract appeared to hinder cell division, increasing the number of interphase cells. In addition, both extracts caused an increase in percentage of cell death. Clastogenic effects...

Tipo: Info:eu-repo/semantics/other

Palavras-chave: Canoparmelia texana; Cytotoxicity; Mitotic index; Myelochroa lindmanii; Plant bioassays.

Ano: 2008

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100024

Cytotoxic and genotoxic potential of surface water from the Pitimbu river, northeastern/RN Brazil

Egito,Lucila Carmem Monte; Medeiros,Maria das Graças; Medeiros,Silvia Regina Batistuzzo de; Agnez-Lima,Lucymara Fassarella.

In this study, the onion (Allium cepa) root test was used to evaluate the genotoxicity of the Pitimbu River (Natal city, Brazil) surface water. The water was collected at five sampling sites along the river and one sample was obtained after the treatment (flocculation, chlorination and pH correction) of the river water for human consumption. All raw river water samples increased the frequency of chromosomal abnormalities and/or micronuclei and two of the water samples produced alterations in the mitotic index of the root cells. Two of the water samples also altered root growth and two produced morphological modifications in the A. cepa roots. Water collected from a site near an industrial area was the most consistently toxic and genotoxic of the samples....

Genotoxic and mutagenic effects of polluted surface water in the midwestern region of Brazil using animal and plant bioassays

Palavras-chave: Allium cepa; Micronucleus; Cytotoxicity; Genotoxicity; Surface water.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300023

Dourado,Priscila Leocádia Rosa; Rocha,Monyque Palagano da; Roveda,Liriana Mara; Raposo Junior,Jorge Luiz; Cândido,Liliam Sílvia; Cardoso,Claudia Andréa Lima; Morales,Maria Aparecida Marin; Oliveira,Kelly Mari Pires de; Grisolia,Alexeia Barufatti.

Abstract This study aimed to evaluate DNA damage in animal and plant cells exposed to water from the Água Boa stream (Dourados, Mato Grosso do Sul, Brazil) by using bioassays, and to identify the chemical compounds in the water to determine the water quality in the area. Through the cytotoxicity bioassay with Allium cepa, using micronucleus test, and comet assay, using Astyanax altiparanae fish, the results indicated that biological samples were genetically altered. Micronuclei were observed in erythrocytes of A. altiparanae after exposure to water from locations close to industrial waste discharge. The highest DNA damage observed with the comet assay in fish occurred with the exposure to water from locations where the presence of metals (Cu, Pb, Cd, Ni)...

Cytotoxicity and genotoxicity of stilbene derivatives in CHO-K1 and HepG2 cell lines

Palavras-chave: Allium cepa; Astyanax altiparanae; Mutagenicity; Cytotoxicity; Genotoxicity.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100123

Mizuno,Cassia Suemi; Ampomaah,Winnifred; Mendonça,Fernanda Ribeiro; Andrade,Gabriela Carvalho; Silva,Ariel Maria Nazaré da; Goulart,Mirian Oliveira; Santos,Raquel Alves dos.

Abstract The cytotoxicity and genotoxicity of the stilbenes (E)-methyl-4-(3-5-dimethoxystyryl)benzoate (ester), (E)-4-(3-5-dimethoxystyryl)aniline (amino), (Z)-1,3-dimethoxy-5-(4-methoxystyryl)benzene (cis-TMS) and (E)-1,3-dimethoxy-5-(4-methoxystyryl)benzene (trans-TMS) were investigated in this work. Structural modifications of resveratrol, a naturally occurring stilbene, have been previously performed, including the replacement of hydroxyl by different functional groups. Such modifications resulted in significant improvement of target-specific effects on cell death and antiproliferative responses. The parameters were evaluated using XTT assay, clonogenic survival assay and the cytokinesis-block micronucleus assay in CHO-K1 and HepG2 cell lines. The...

Évaluation d’une suite de bio-essais pour la détection et l’étude de composés lipophiles de micro-organismes marins issus de mollusques bivalves et de leur environnement

Palavras-chave: Stilbene; Cytotoxicity; Genotoxicity; Micronucleus assay.

Ano: 2017

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400656

Cytotoxic and antibacterial properties of Mytilus galloprovincialis, Ostrea edulis and Crassostrea gigas (bivalve molluscs) hemolymph

Geiger, Marie.

The context of this study was the assessment of the safety of edible bivalve molluscs. Bivalve can indeed accumulate high concentrations of phycotoxins into their hepatopancreas, potentially leading to human intoxication outbreaks after consumption of such contaminated bivalves. In order to ensure the protection of shellfish consumers, regulated toxins are monitored using physico-chemical techniques. In parallel, emerging toxins, that can be putatively produced by microalgae or marine fungi, are monitored using the intraperitoneal mouse bioassay. However, this bioassay suffers ethical and methodological drawbacks. Thus, the objective of this work was to assess the ability of a bioassay suite to detect lipophilic toxins. This suite was composed of three...

Tipo: Text

Palavras-chave: Cytotoxicité; Larves de diptères; Activité antibactérienne; Toxines lipophiles; Micro-algues; Dinoflagellés; Micromycètes; Penicillium; Acide okadaïque; Azaspiracide; Pinnatoxine G; Vulcanodinium rugosum; Prorocentrum lima; Azadinium spinosum; Beauveria brongniartii; Cytotoxicity; Diptera larvae; Antibacterial activity; Lipophilic toxins; Microalgae; Dinoflagellates; Marine fungi; Penicillium; Okadaic acid; Azaspiracid; Pinnatoxin G; Vulcanodinium rugosum; Azadinium spinosum; Prorocentrum lima; Beauveria brongniartii.

Ano: 2013

URL: http://archimer.ifremer.fr/doc/00183/29426/27787.pdf

A rapid quantitative fluorescence-based bioassay to study allelochemical interactions from Alexandrium minutum

Hubert, Florence; Van Der Knaap, Wil; Noel, Thierry; Roch, Philippe.

Mussel (Mytilus galloprovincialis) plasma contains cytotoxic activity against both vertebrate (erythrocytes and mouse tumour) and protozoan cells. Procaryotes (Escherichia coli and Vibrio alginolyticus) were not sensitive to the cytotoxicity. The activity was still present in dialyzed samples but was inhibited by heating at 45°C. Large individual variability which was not correlated with protein concentration and an increasing number of reactive specimens following injection, suggested that naive mussels were in various stages of immune response. Purification by anion exchange chromatography followed by gel filtration revealed a 320 kDa cytotoxic polymeric protein that acts through a polymerization process after binding onto target cell membranes as...

Tipo: Text

Palavras-chave: Cytotoxicité; Antibactérien; Hémolymphe; Lysat d'hémocyte; Moule; Huître; Mytilus galloprovincialis; Ostrea edulis; Crassostrea gigas; Cytotoxicity; Antibacterial; Hemolymph; Hemocyte lysate; Musssel; Oyster; Mytilus galloprovincialis; Ostrea edulis; Crassostrea gigas.

Ano: 1996

URL: http://archimer.ifremer.fr/doc/00039/15008/12333.pdf

Cytotoxicity and mycotoxin production of shellfish-derived Penicillium spp., a risk for shellfish consumers

Long, Marc; Tallec, Kevin; Soudant, Philippe; Lambert, Christophe; Le Grand, Fabienne; Sarthou, Geraldine; Jolley, Dianne; Hegaret, Helene.

Harmful microalgal blooms are a threat to aquatic organisms, ecosystems and human health. Toxic dinoflagellates of the genus Alexandrium are known to produce paralytic shellfish toxins and to release bioactive extracellular compounds (BECs) with potent cytotoxic, hemolytic, ichtyotoxic and allelopathic activity. Negative allelochemical interactions refer to the chemicals that are released by the genus Alexandrium and that induce adverse effects on the physiology of co-occurring protists and predators. Releasing BECs gives the donor a competitive advantage that may help to form dense toxic blooms of phytoplankton. However BECs released by Alexandrium minutum are uncharacterized and it is impossible to quantify them using classical chemical methods....

Tipo: Text

Palavras-chave: Harmful algal bloom; Alexandrium; Allelopathy; Cytotoxicity; PAM fluorometry; Bioassay.

Ano: 2018

URL: https://archimer.ifremer.fr/doc/00453/56424/58116.pdf

Polystyrene microbeads modulate the energy metabolism of the marine diatom Chaetoceros neogracile

Geiger, Marie; Guitton, Yann; Vansteelandt, M.; Kerzaon, I.; Blanchet, Estelle; Du Pont, T. Robiou; Frisvad, J. C.; Hess, Philipp; Pouchus, Y. F.; Grovel, Olivier.

In order to assess the putative toxigenic risk associated with the presence of fungal strains in shellfish-farming areas, Penicillium strains were isolated from bivalve molluscs and from the surrounding environment, and the influence of the sample origin on the cytotoxicity of the extracts was evaluated. Extracts obtained from shellfish-derived Penicillia exhibited higher cytotoxicity than the others. Ten of these strains were grown on various media including a medium based on mussel extract (Mytilus edulis), mussel flesh-based medium (MES), to study the influence of the mussel flesh on the production of cytotoxic compounds. The MES host-derived medium was created substituting the yeast extract of YES medium by an aqueous extract of mussel tissues, with...

Tipo: Text

Palavras-chave: Cytotoxicity; Dereplication; Marine fungi; Mass spectrometry; Penicillium; Shellfish.

Ano: 2013

URL: http://archimer.ifremer.fr/doc/00160/27166/25523.pdf

Reduction of adhesion properties of Ruditapes philippinarum hemocytes exposed to Vibrio tapetis

Seoane, Marta; González-fernández, Carmen; Soudant, Philippe; Huvet, Arnaud; Esperanza, Marta; Cid, Ángeles; Paul-pont, Ika.

Due to the growing concern about the presence of microplastics (MP) in the environment, the number of studies evaluating the toxicity of these small persistent particles on different marine species has increased in recent years. Few studies have addressed their impact on marine phytoplankton, a subject of great concern since they are primary producers of the aquatic food web. The aim of this study is to unravel the cytotoxicity of 2.5 μg mL−1 unlabelled amino-modified polystyrene beads of different sizes (0.5 and 2 μm) on the marine diatom Chaetoceros neogracile. In addition to traditional growth and photosynthesis endpoints, several physiological and biochemical parameters were monitored every 24 h in C. neogracile cells by flow cytometry during their...

Tipo: Text

Palavras-chave: Microalgae; Microplastic; Flow cytometry; Cytotoxicity; Scanning electron microscopy.

Ano: 2019

URL: https://archimer.ifremer.fr/doc/00492/60415/63850.pdf

Bioactive Bromotyrosine-Derived Alkaloids from the Polynesian Sponge Suberea ianthelliformis

Choquet, Gwenaelle; Soudant, P; Lambert, C; Nicolas, Jean-louis; Paillard, C.

Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of other Vibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V tapetis compared to that of V splendidus and V pectenicida suggests a specific pathogenicity...

Tipo: Text

Palavras-chave: Cytotoxicity; Flow cytometry; Vibrio tapetis; Ruditapes philippinarum; Bivalve hemocytes; Pathogenicity.

Ano: 2003

URL: http://archimer.ifremer.fr/doc/00000/10761/7590.pdf