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| Registros recuperados: 30 | |
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| Jauffrais, Thierry; Lekieffre, Charlotte; Schweizer, Magali; Jesus, Bruno; Metzger, Edouard; Geslin, Emmanuelle. |
| The aim of this work is to document the complex nutritional strategy developed by kleptoplastic intertidal foraminifera. We study the mixotrophic ability of a common intertidal foraminifer, E. williamsoni, by (1) investigating the phylogenetic identity of the foraminiferal kleptoplasts, (2) following their oxygenic photosynthetic capacity, and (3) observing the modification in cellular ultrastructural features in response to photoautotrophic conditions. This was achieved by coupling molecular phylogenetic analyses and TEM observations with non-destructive measurements of kleptoplast O2 production over a 15-day experimental study. Results show that the studied E. williamsoni actively selected kleptoplasts mainly from pennate diatoms and had the ability to... |
| Tipo: Text |
Palavras-chave: Foraminifera; Electron microscopy; DNA barcoding; Kleptoplasty; Lipids. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00487/59894/63074.pdf |
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| Delrieu‐trottin, Erwan; Durand, Jean‐dominique; Limmon, Gino; Sukmono, Tedjo; Kadarusman,; Sugeha, Hagi Yulia; Chen, Wei‐jen; Busson, Frédéric; Borsa, Philippe; Dahruddin, Hadi; Sauri, Sopian; Fitriana, Yuli; Zein, Mochamad Syamsul Arifin; Hocdé, Régis; Pouyaud, Laurent; Keith, Philippe; Wowor, Daisy; Steinke, Dirk; Hanner, Robert; Hubert, Nicolas. |
| DNA barcoding opens new perspectives on the way we document biodiversity. Initially proposed to circumvent the limits of morphological characters to assign unknown individuals to known species, DNA barcoding has been used in a wide array of studies where collecting species identity constitutes a crucial step. The assignment of unknowns to knowns assumes that species are already well identified and delineated, making the assignment performed reliable. Here, we used DNA‐based species delimitation and specimen assignment methods iteratively to tackle the inventory of the Indo‐Australian Archipelago grey mullets, a notorious case of taxonomic complexity that requires DNA‐based identification methods considering that traditional morphological identifications... |
| Tipo: Text |
Palavras-chave: Coral Triangle; Cryptic diversity; DNA barcoding; Reference library; Taxonomic gap. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00606/71810/70300.pdf |
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| Griffiths, Andrew M.; Sotelo, Carmen G.; Mendes, Rogerio; Perez-martin, Ricardo I.; Schroeder, Ute; Shorten, Marc; Silva, Helena A.; Verrez-bagnis, Veronique; Mariani, Stefano. |
| Mislabelling of food products has recently received a great deal of public scrutiny, but it remains unclear exactly what methods are being utilised in laboratories testing the authenticity of foods. In order to gain insight into the specific area of the analysis of seafood, a questionnaire focusing on the taxonomic groups typically analysed and the techniques utilised was sent to over one hundred accredited laboratories across the UK, Ireland, Spain, Portugal, France and Germany. Forty-five responded positively, demonstrating significant differences in both the species analysed and methods utilised among the countries included in the survey. Indeed, a diversity of methods was employed across laboratories and efforts to harmonise and/or standardise testing... |
| Tipo: Text |
Palavras-chave: Food testing; Forensically informative nucleotide sequencing; Species identification; DNA barcoding; Fisheries. |
| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00188/29918/28383.pdf |
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| Sevilla, Rafael G.; Diez, Amalia; Noren, Michael; Mouchel, Olivier; Jerome, Marc; Verrez-bagnis, Veronique; Van Pelt, Hilde; Favre Krey, Laurence; Krey, Grigorios; Bautista, José M.. |
| This report describes a set of 21 polymerase chain reaction primers and amplification conditions developed to barcode practically any teleost fish species according to their mitochondrial cytochrome b and nuclear rhodopsin gene sequences. The method was successfully tested in more than 200 marine fish species comprising the main Actinopterygii family groups. When used in phylogenetic analyses, its combination of two genes with different evolutionary rates serves to identify fish at the species level. We provide a flow diagram indicating our validated polymerase chain reaction amplification conditions for barcoding and species identification applications as well as population structure or haplotyping analyses, adaptable to high-throughput analyses. |
| Tipo: Text |
Palavras-chave: Teleost; Rhodopsin; PCR primers; Mitochondrial cytochrome b; Fish; DNA barcoding. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3034.pdf |
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| Seifert, K.A.; Gams, W.. |
| This supplement to the taxonomic monograph The Genera of Hyphomycetes summarises information on 23 accepted new genera and c. 160 species described in 2011. These include three dematiaceous genera (Funbolia, Noosia, Pyrigemmula, all related to Dothideomycetes), a bulbil-producing genus, Spiroplana (Pleosporales), and two endophytic genera, the sterile Periglandula (Clavicipitaceae), and the hyaline, sympodial Micronematobotrys (Pyronemataceae). Slow-growing, morphologically-reduced, darkly pigmented fungi continue to be the source of new taxa, including the new genus Atramixtia (Dothioraceae). Eight new genera of darkly pigmented chlamydosporelike anamorphs were described from marine or subtidal environments (Glomerulispora, Halozoön, Hiogispora,... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Anamorphic fungi; DNA barcoding; Moulds; New genera; New species. |
| Ano: 2012 |
URL: http://www.repository.naturalis.nl/record/532291 |
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| Randrianiaina, R.-D.; Strauß, A.; Glos, J.; Glaw, F.; Vences, M.. |
| We provide detailed morphological descriptions of the tadpoles of Malagasy river bank frogs of the subgenera Ochthomantis and Maitsomantis (genus Mantidactylus, family Mantellidae), and data on relative abundance and habitat preferences of Ochthomantis species from Ranomafana National Park in southeastern Madagascar. Our study includes the tadpoles of six described and four undescribed candidate species. Eight of these larvae were previously unknown. Tadpoles were identified by DNA barcoding. Due to the very rudimentary taxonomic knowledge on Ochthomantis, we followed a ‘reverse taxonomy’ approach in which adult classification was to a great part determined on the basis of larval differences. By this procedure we even identified one candidate species whose... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Amphibia; Mantellidae; Madagascar; Tadpole morphology; DNA barcoding; 42.82. |
| Ano: 2011 |
URL: http://www.repository.naturalis.nl/record/369710 |
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| Morgado, L.N.; Noordeloos, M.E.; Lamoureux, Y.; Gem, J.. |
| Species from Entoloma subg. Entoloma are commonly recorded from both the Northern and Southern Hemispheres and, according to literature, most of them have at least Nearctic-Palearctic distributions. However, these records are based on morphological analysis, and studies relating morphology, molecular data and geographical distribution have not been reported. In this study, we used phylogenetic species recognition criteria through gene genealogical concordance (based on nuclear ITS, LSU, rpb2 and mitochondrial SSU) to answer specific questions considering species limits in Entoloma subg. Entoloma and their geographic distribution in Europe, North America and Australasia. The studied morphotaxa belong to sect. Entoloma, namely species like the notorious... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Biodiversity; DNA barcoding; Entolomataceae; Fungal biogeography; Molecular systematics; Phylogenetic species. |
| Ano: 2013 |
URL: http://www.repository.naturalis.nl/record/532443 |
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| Nieukerken, E.J. van; Mutanen, M.; Doorenweerd, C.. |
| We sequenced the mitochondrial barcoding marker COI and nuclear marker EF1-alpha for most Nordic and other European species of the Stigmella salicis and S. aurella species groups. In the S. salicis group both markers confirm the synonymy of S. lappovimella with S. zelleriella. Specimens previously identified as Stigmella salicis and S. vimineticola are shown to form a complex of several cryptic species for which the taxonomy needs to be worked out. The species previously recorded as S. vimineticola from Norway represents probably an unnamed species. In the S. aurella group, the oligophagous Rosaceae feeders S. aurella and S. poterii are confirmed to be each a single oligophagous species. The synonymy between Stigmella ulmariae from Filipendula ulmaria and... |
| Tipo: Article / Letter to the editor |
Palavras-chave: DNA barcoding; Phylogeny; Lepidoptera; 42.75. |
| Ano: 2012 |
URL: http://www.repository.naturalis.nl/record/417740 |
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| Walther, G.; Pawłowska, J.; Alastruey-Izquierdo, A.; Wrzosek, M.; Rodriguez-Tudela, J.L.; Dolatabadi, S.; Chakrabarti, A.; Hoog, G.S. de. |
| The order Mucorales comprises predominantly fast-growing saprotrophic fungi, some of which are used for the fermentation of foodstuffs but it also includes species known to cause infections in patients with severe immune or metabolic impairments. To inventory biodiversity in Mucorales ITS barcodes of 668 strains in 203 taxa were generated covering more than two thirds of the recognised species. Using the ITS sequences, Molecular Operational Taxonomic Units were defined by a similarity threshold of 99 %. An LSU sequence was generated for each unit as well. Analysis of the LSU sequences revealed that conventional phenotypic classifications of the Mucoraceae are highly artificial. The LSU- and ITS-based trees suggest that characters, such as rhizoids and... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Backusella; Biodiversity; Clinical relevance; DNA barcoding; Intraspecific variability; ITS; LSU; Mucor; Mucorales; Nomenclature; Rhizomucor; Taxonomy; Zygorhynchus. |
| Ano: 2013 |
URL: http://www.repository.naturalis.nl/record/532353 |
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| Schmid-Egger, C.; Achterberg, K. van; Neumeyer, R.; Morinière, J.; Schmidt, S.. |
| The genus Polistes is revised for the West Palaearctic region based on morphology and DNA barcodes. The revision includes all known West Palaearctic species, raising the number of species in Europe to 14 and to 17 for the West Palaearctic realm. DNA barcodes were recovered from 15 species, 14 of which belong to the subgenus Polistes, and one, P. wattii, to the subgenus Gyrostoma. An integrative taxonomic approach combining morphology and molecular data (DNA barcoding) was employed to resolve longstanding taxonomic problems in this group. Two species, P. austroccidentalis van Achterberg & Neumeyer, sp. n. (= P. semenowi auctt.) from W and SW Europe and P. maroccanus Schmid-Egger, sp. n. from Morocco are described as new. Polistes bucharensis Erichson,... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Taxonomic revision; DNA barcoding; Key to species; Integrative taxon. |
| Ano: 2017 |
URL: http://www.repository.naturalis.nl/record/644038 |
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| Veldman, S.. |
| Medicinal plants are an important source of primary healthcare for many people in Tanzania. These medicinal plants are harvested from the wild, and increasing commercial trade poses a serious threat to local plant populations. Currently it is unknown which species are traded and in what amounts. Across the southwestern border in Zambia, the traditional dish chikanda has transformed from a niche product to being a mainstream delicacy. One of the main ingredients are wild-harvested orchids, and these have become depleted throughout the country as an effect of the increased trade. It is unclear which orchid species are targeted and might be at risk of overharvesting. The aims of my doctorate are to map harvest and trade of Tanzanian medicinal plants and... |
| Tipo: Book (monograph) |
Palavras-chave: DNA barcoding; Africa; Species delimitation; Orchids; Medicinal plants. |
| Ano: 2018 |
URL: http://www.repository.naturalis.nl/record/668031 |
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| Meij, S.E.T. van der; Berumen, M.L.; Paulay, G.. |
| A new species of cryptochirid crab, Fizesereneia panda van der Meij, is described and illustrated based on specimens collected from the scleractinian corals Lobophyllia cf. hemprichii and L. cf. corymbosa from the Farasan Banks, Farasan Islands, and the reefs off Thuwal in the Saudi Arabian Red Sea, and from Symphyllia recta from reefs in the Gulf of Oman. This is the second cryptochirid species with the Red Sea as type locality. It can be separated from its congeners by the subrectangular carapace, raised midline and the complete division of the carapace depressions, and reddish black colour pattern of these concavities in live specimens. This new species is the seventh assigned to Fizesereneia. A DNA barcode for the new species has been deposited in... |
| Tipo: Article / Letter to the editor |
Palavras-chave: DNA barcoding; Gall crabs; Host specificity; Lobophylliidae; Saudi Arabia; Scleractinia; Taxonomy; 42.74. |
| Ano: 2015 |
URL: http://www.repository.naturalis.nl/record/533931 |
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| Wall-Palmer, D.; Burridge, A.K.; Peijnenburg, K.C.T.A.; Janssen, A.; Goetze, E.; Kirby, R.; Hart, M.B.; Smart, C.W.. |
| The genus Protatlanta is thought to be monotypic and is part of the Atlantidae, a family of shelled heteropods. These microscopic planktonic gastropods are poorly known, although research on their ecology is now increasing in response to concerns about the effects of ocean acidification on calcareous plankton. A correctly implemented taxonomy of the Atlantidae is fundamental to this progressing field of research and it requires much attention, particularly using integrated molecular and morphological techniques. Here we use DNA barcoding, shell morphology and biogeography to show that the genus Protatlanta includes at least two valid species in the Atlantic Ocean. Protatlanta souleyeti and Protatlanta sculpta were found to be separate species, with... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Atlantic Ocean; Biogeography; DNA barcoding; Morphometrics; Protatlanta; Shelled heteropod. |
| Ano: 2016 |
URL: http://www.repository.naturalis.nl/record/619150 |
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| Orihara, T.; Lebel, T.; Ge, Z.-W.; Smith, M.E.; Maekawa, N.. |
| The sequestrate (truffle-like) basidiomycete genera Rossbeevera, Chamonixia, and Octaviania are closely related to the epigeous mushroom genera Leccinum and Leccinellum. In order to elucidate the properties and placement of several undescribed sequestrate taxa in the group and to reveal the evolutionary history of Rossbeevera and its allies, we conducted phylogenetic analyses based on three nuclear (ITS, nLSU, EF-1α) and two mitochondrial DNA loci (ATP6 and mtSSU) as well as precise morphological observations. Phylogenetic analyses of three nuclear loci suggest a complex evolutionary history with sequestrate fruiting bodies present in several clades, including a previously unrecognized sister clade to Rossbeevera. Here we propose a new sequestrate genus,... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Biogeography; Cryptic species; DNA barcoding; Hypogeous fungi; Introgression; Species tree. |
| Ano: 2016 |
URL: http://www.repository.naturalis.nl/record/611857 |
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| Mutanen, M.; Kivelä, S.M.; Vos, R.A.; Doorenweerd, C.; Ratnasingham, S.; Hausmann, A.; Huemer, P.; Dinca, V.; Nieukerken, E.J. van; Lopez-Vaamonde, C.; Vila, R.; Aarvik, L.; Decaëns, T.; Efetov, K.A.; Hebert, P.D.N.; Johnsen, A.; Karsholt, O.; Pentinsaari, M.; Rougerie, R.; Segerer, A.; Tarmann, G.; Zahiri, R.; Godfray, H.C.J.. |
| The proliferation of DNA data is revolutionizing all fields of systematic research. DNA barcode sequences, now available for millions of specimens and several hundred thousand species, are increasingly used in algorithmic species delimitations. This is complicated by occasional incongruences between species and gene genealogies, as indicated by situations where conspecific individuals do not form a monophyletic cluster in a gene tree. In two previous reviews, non-monophyly has been reported as being common in mitochondrial DNA gene trees. We developed a novel web service “Monophylizer” to detect non-monophyly in phylogenetic trees and used it to ascertain the incidence of species non-monophyly in COI (a.k.a. cox1) barcode sequence data from 4977 species... |
| Tipo: Article / Letter to the editor |
Palavras-chave: DNA barcoding; Gene tree; Lepidoptera; Mitochondrial COI; Mitochondrial cox1; Paraphyly; Polyphyly; Species delimitation; Species monophyly. |
| Ano: 2016 |
URL: http://www.repository.naturalis.nl/record/648758 |
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| Martin, P.; Dorn, N.J.; Kawai, T.; Heiden, C. van der; Scholtz, G.. |
| A mysterious parthenogenetic cambarid crayfish (the Marmorkrebs) has been spreading across the globe for the past decade. We compare this crayfish directly to two other cambarids, Procambarus fallax and P. alleni, that have been suggested to be related or even identical to the Marmorkrebs. Using external morphology and sequences of two mitochondrial genes we show clear correspondences between Marmorkrebs and P. fallax, a species found natively throughout peninsular Florida, USA. Based on these congruent results we suggest that the Marmorkrebs is the parthenogenetic form of P. fallax. This finding has potential evolutionary and ecological implications at several levels. The Marmorkrebs might be a type of geographical parthenogenesis, but a natural... |
| Tipo: Article / Letter to the editor |
Palavras-chave: 12S rRNA; Annulus ventralis; COI; DNA barcoding; Species concept; Thelytoky; 42.74. |
| Ano: 2010 |
URL: http://www.repository.naturalis.nl/record/355212 |
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| Stielow, J.B.; Lévesque, C.A.; Seifert, K.A.; Meyer, W.; Irinyi, L.; Smits, D.; Renfurm, R.; Verkley, G.J.M.; Groenewald, M.; Chaduli, D.; Lomascolo, A.; Welti, S.; Lesage-Meessen, L.; Favel, A.; Al-Hatmi, A.M.S.; Damm, U.; Yilmaz, N.; Houbraken, J.; Lombard, L.; Quaedvlieg, W.; Binder, M.; Vaas, L.A.I.; Vu, D.; Yurkov, A.; Begerow, D.; Roehl, O.; Guerreiro, M.; Fonseca, A.; Samerpitak, K.; Diepeningen, A.D. van; Dolatabadi, S.; Moreno, L.F.; Casaregola, S.; Mallet, S.; Jacques, N.; Roscini, L.; Egidi, E.; Bizet, C.; Garcia-Hermoso, D.; Martin, M.P.; Deng, S.; Groenewald, J.Z.; Boekhout, T.; Beer, Z.W. de; Barnes, I.; Duong, T.A.; Wingfield, M.J.; Hoog, G.S. de; Crous, P.W.; Lewis, C.T.; Hambleton, S.; Moussa, T.A.A.; Al-Zahrani, H.S.; Almaghrabi, O.A.; Louis-Seize, G.; Assabgui, R.; McCormick, W.; Omer, G.; Dukik, K.; Cardinali, G.; Eberhardt, U.; Vries, M. de; Robert, V.. |
| The aim of this study was to assess potential candidate gene regions and corresponding universal primer pairs as secondary DNA barcodes for the fungal kingdom, additional to ITS rDNA as primary barcode. Amplification efficiencies of 14 (partially) universal primer pairs targeting eight genetic markers were tested across > 1 500 species (1 931 strains or specimens) and the outcomes of almost twenty thousand (19 577) polymerase chain reactions were evaluated. We tested several well-known primer pairs that amplify: i) sections of the nuclear ribosomal RNA gene large subunit (D1–D2 domains of 26/28S); ii) the complete internal transcribed spacer region (ITS1/2); iii) partial β-tubulin II (TUB2); iv) γ-actin (ACT); v) translation elongation factor 1-α (TEF1α);... |
| Tipo: Article / Letter to the editor |
Palavras-chave: DNA barcoding; ITS supplement; Molecular taxonomy; Phylogeny; Species identification; Universal primers. |
| Ano: 2015 |
URL: http://www.repository.naturalis.nl/record/588731 |
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| Registros recuperados: 30 | |
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