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| Registros recuperados: 46 | |
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| KAWASHIMA, Chiho; MUNAKATA, Megumi; SHIMIZU, Takashi; MIYAMOTO, Akio; KIDA, Katsuya; MATSUI, Motozumi; 川島, 千帆; 清水, 隆; 宮本, 明夫; 木田, 克弥; 松井, 基純. |
| This study aimed to investigate the effects of insulin resistance (IR) during the close-up dry period on the metabolic status and performance of dairy cows as well as to determine the effects on body weight (BW) and metabolic status of their calves. An insulin tolerance test (ITT) was conducted by administering 0.05 IU/kg BW of insulin to 34 multiparous Holstein cows at 3 weeks prepartum. Blood samples were collected at 0, 30, 45 and 60 min after insulin injection, and cows were divided into two groups based on the time required for glucose to reach the minimum levels [non-IR (NIR), 45 min (n=28); and IR, 60 min (n=6)]. Blood or milk sampling and body condition score (BCS) estimation were performed twice weekly during the experimental period. Blood samples... |
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Palavras-chave: Dairy cow; Dry period; Insulin tolerance test; Metabolic status; Postpartum performance. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4363 |
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| MAGATA, Fumie; ISHIDA, Yoshikazu; MIYAMOTO, Akio; FURUOKA, Hidefumi; INOKUMA, Hisashi; SHIMIZU, Takashi; 宮本, 明夫; 古岡, 秀文; 猪熊, 壽; 清水, 隆. |
| We investigated the concentration of the bacterial endotoxin lipopolysaccharide (LPS) in the blood, ovarian follicular fluid and uterine fluid of a clinical case of bovine metritis. A 2-year-old lactating Holstein cow exhibited continuous fever >39.5°C for more than 2 weeks after normal calving. The cow produced a fetid, watery, red-brown uterine discharge from the vagina and was diagnosed with metritis. The LPS concentrations in plasma and uterine fluid were 0.94 and 6.34 endotoxin units (EU)/ml, respectively. One of seven follicles showed an extremely high level of LPS (12.40 EU/ml) compared to the other follicles (0.62–0.97 EU/ml). These results might suggest the presence of high concentration of LPS in follicles in cows with postpartum metritis. |
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Palavras-chave: Dairy cow; Follicle; Lipopolysaccharide; Metritis. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4043 |
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| Kawashima, Chiho; Karaki, Chihiro; Munakata, Megumi; Matsui, Motozumi; Shimizu, Takashi; Miyamoto, Akio; Kida, Katsuya. |
| Recent studies have shown significant associations between prepartum energy status and postpartum fertility in dairy cows; therefore, the assessment of energy status by blood metabolites and metabolic hormones and suitable improvement of management during the prepartum period may enhance reproductive performance. Rumen fill score (RFS) is associated with feed intake; however, it is unknown whether RFS is also related to blood parameters. Therefore, this study investigated the relationship between RFS and energy status during the prepartum period, and their associations with conception at first artificial insemination (AI) after parturition. In 42 multiparous Holstein cows, RFS assessment and blood sampling were carried out twice a week during 3 weeks of... |
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Palavras-chave: Dairy cow; Dry period; First artificial insemination; Rumen fill score. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4529 |
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| MAGATA, Fumie; HORIUCHI, Maya; MIYAMOTO, Akio; SHIMIZU, Takashi; 宮本, 明夫; 清水, 隆. |
| In postpartum dairy cows, lipopolysaccharide (LPS) derived from gram-negative bacteria such as Escherichia coli causes uterine inflammation and leads to ovarian dysfunction. The aim of this study was to determine the effect of LPS on steroid production in bovine theca cells at different stages of follicular development. Theca cells isolated from pre- and post-selection follicles (PRFs, <8.5 mm in diameter, and POFs, >8.5 mm in diameter, respectively) of bovine ovaries were exposed to LPS under luteinizing hormone (LH) conditions, estradiol (E2) conditions or both conditions in vitro. Bovine theca cells expressed the LPS receptor gene complex: Toll-like receptor 4 (TLR4), CD14 and MD2. LPS suppressed progesterone (P4) and androstenedione (A4)... |
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Palavras-chave: Androstenedione; Dairy cow; Lipopolysaccharide; Progesterone; Theca cells. |
| Ano: 2014 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3935 |
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| MAGATA, Fumie; SHIRASUNA, Koumei; STRÜVE, Klaas; HERZOG, Kathrin; SHIMIZU, Takashi; BOLLWEIN, Heinrich; MIYAMOTO, Akio; 清水, 隆; 宮本, 明夫. |
| Persistence of the corpus luteum (CL) in cattle usually occurs during the puerperium and is associated with interference of prostaglandin (PG) F2α release from the uterus. The objective of the present study was to determine for the first time the gene expressions in the persistent CL compared with the CL of pregnancy and cyclic CL. Three types of CL biopsy samples were collected from 32 lactating Holstein cows: (1) CL persisting for 29 to 33 days after the first ovulation postpartum (persistent CL, n=9), (2) CL between days 29 and 33 of early pregnancy (CL of pregnancy, n=8) and (3) CL between days 10 and 13 of the estrous cycle (cyclic CL, n=27). mRNA expression of 2',5'-oligoadenylate synthetase-1 was upregulated only in the CL of pregnancy, confirming... |
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Palavras-chave: Dairy cow; Early pregnancy; Persistent corpus luteum; MRNA expression. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3938 |
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| KAWASHIMA, Chiho; ITO, Nozomi; NAGASHIMA, Shuntarou; MATSUI, Motozumi; SAWADA, Kumiko; SCHWEIGERT, Florian J.; MIYAMOTO, Akio; KIDA, Katsuya; 川島, 千帆; 松井, 基純; 宮本, 明夫; 木田, 克弥. |
| The aim of the present study was to investigate nutritional and metabolic parameters during the dry and early postpartum periods of ovulatory and anovulatory cows, as well as their postpartum reproductive performance. Blood samples from 20 multiparous Holstein cows were collected once a week from the far-off dry period to 3 weeks postpartum. Early postpartum (0–3 weeks) ovulation was confirmed using plasma progesterone concentration profiles, and cows were considered ovulatory if they had resumed luteal activity by this point (n = 9), whereas cows that had not were considered anovulatory (n = 11). Data from the ovulatory and anovulatory cows were analyzed separately for the far-off dry period (7–4 weeks prepartum), the close-up dry period (3–1 weeks... |
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Palavras-chave: Dairy cow; First ovulation; Hepatic enzyme; Liver function; Reproductive performance. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4308 |
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| KAWASHIMA, Chiho; KANEKO, Etsushi; AMAYA MONTOYA, Carlos; MATSUI, Motozumi; YAMAGISHI, Norio; MATSUNAGA, Nobuyoshi; ISHII, Mitsuo; KIDA, Katsuya; MIYAKE, Yoichi; MIYAMOTO, Akio; 川島, 千帆; 松井, 基純; 石井, 三都夫; 木田, 克弥; 三宅, 陽一; 宮本, 明夫. |
| The aim of this study was to investigate the relationship between the first ovulation within 3 weeks postpartum and subsequent ovarian cycles and fertility in high producing dairy cattle in Hokkaido, Japan. In Experiment 1, 110 cows (44 primiparous and 66 multiparous) were used to determine the effects of the first ovulation within 3 weeks postpartum on subsequent ovarian cycles. Milk samples were collected twice weekly from 7 to 100 days postpartum. The first ovulation was identified by an increase in milk progesterone (P4) to more than 1 ng/ml within 3 weeks postpartum. The numbers of cows showing ovulation and anovulation within 3 weeks postpartum were 31 (70.5%) and 13 (29.5%) in the primiparous cows and 35 (53.0%) and 31 (47.0%) in the multiparous... |
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Palavras-chave: Dairy cow; First ovulation; Postpartum; Resumption of ovarian cycle. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/916 |
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| MAETANI, Ayami; ITOH, Megumi; NISHIHARA, Kahori; AOKI, Takahiro; OHTANI, Masayuki; SHIBANO, Kenichi; KAYANO, Mitsunori; YAMADA, Kazutaka; 伊藤, めぐみ; 滄木, 孝弘; 芝野, 健一; 茅野, 光範; 山田, 一孝. |
| The aim of this study was to assess the measurement of bone mineral density (BMD) by quantitative computed tomography (QCT), comparing the relationships of BMD between QCT and dual-energy X-ray absorptiometry (DXA) and between QCT and radiographic absorptiometry (RA) in the metacarpal bone of Holstein dairy cows (n=27). A significant positive correlation was found between QCT and DXA measurements (r=0.70, P<0.01), and a significant correlation was found between QCT and RA measurements (r=0.50, P<0.01). We conclude that QCT provides quantitative evaluation of BMD in dairy cows, because BMD measured by QCT showed positive correlations with BMD measured by the two conventional methods: DXA and RA. |
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Palavras-chave: Bone mineral density; Dairy cow; Dual-energy X-ray absorptiometry; Quantitative computed tomography; Radiographic absorptiometry. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4321 |
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| MAGATA, Fumie; SHIRASUNA, Koumei; STRÜVE, Klaas; HERZOG, Kathrin; SHIMIZU, Takashi; BOLLWEIN, Heinrich; MIYAMOTO, Akio; 清水, 隆; 宮本, 明夫. |
| Persistence of the corpus luteum (CL) in cattle usually occurs during the puerperium and is associated with interference of prostaglandin (PG) F2α release from the uterus. The objective of the present study was to determine for the first time the gene expressions in the persistent CL compared with the CL of pregnancy and cyclic CL. Three types of CL biopsy samples were collected from 32 lactating Holstein cows: (1) CL persisting for 29 to 33 days after the first ovulation postpartum (persistent CL, n=9), (2) CL between days 29 and 33 of early pregnancy (CL of pregnancy, n=8) and (3) CL between days 10 and 13 of the estrous cycle (cyclic CL, n=27). mRNA expression of 2',5'-oligoadenylate synthetase-1 was upregulated only in the CL of pregnancy, confirming... |
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Palavras-chave: Dairy cow; Early pregnancy; Persistent corpus luteum; MRNA expression. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4039 |
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| KAWASHIMA, Chiho; MATSUI, Motozumi; SHIMIZU, Takashi; KIDA, Katsuya; MIYAMOTO, Akio; 川島, 千帆; 松井, 基純; 清水, 隆; 木田, 克弥; 宮本, 明夫. |
| During recent decades, milk production per cow has increased drastically due to improved management, nutrition, and genetic selection; however, the reproductive performance of high-producing dairy cows has been declining. One of the factors responsible for this low reproductive performance is negative energy balance (NEB). NEB affects the onset of first ovulation in early postpartum cows. It is generally accepted that early first ovulation positively relates to the resumption of normal ovarian function, first service, and conception rate in dairy cows. Hence, delayed first ovulation has a negative impact on subsequent fertility. The metabolic condition of cows in NEB shifts to catabolic metabolism, which in turn causes increased plasma growth hormone and... |
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Palavras-chave: Β-Carotene; Dairy cow; First follicular wave postpartum; First ovulation; Metabolic hormone. |
| Ano: 2012 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4041 |
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| Miguel,Marcolino Frederico; Ribeiro Filho,Henrique Mendonça Nunes; Crestani,Steben; Ramos,Fabiana da Rocha; Genro,Tereza Cristina Moraes. |
| The objective of this work was to assess the effects of the sward structure of Italian ryegrass (Lolium multiflorum), during the first grazing cycle, on its morphological and bromatological characteristics throughout the growing season, and on the performance of dairy cows. The treatments consisted of two structures obtained as a function of canopy-light interception: high-light interception (HLI) and low-light interception (LLI), with different pre-grazing heights in the first grazing cycle. Pasture was managed under rotational grazing with a herbage allowance not below 30 kg dry matter (DM) per cow per day. Three grazing cycles, with a grazing interval of 30 days, were evaluated. Pre-grazing herbage mass was greater (2,240 vs. 1,656 kg ha-1 DM), but the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lolium multiflorum; Dairy cow; Herbage intake; Sward structure. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000600018 |
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| Castaño,Gastón Adolfo; Pabón,Martha Lucía; Carulla,Juan Evangelista. |
| The changes in the concentration of trans-vaccenic (C18:1t-11) and rumenic (C18:2c-9,t-11) acids in the milk from cows grazing on Pennisetum clandestinum, fed a supplement containing palm oil, rice bran or whole cottonseed were evaluated. Three supplements were assessed: one control supplement containing palm oil (C), with a low concentration of linoleic acid mainly from palm oil, and two supplements containing rice bran (RB) or whole cottonseed (CS) as the main source of linoleic acid. Six Holstein cows (4.2±1.7 years of age, 532.5±50.7 kg BW, 125±29 days in milk and a milk yield of 21.7 5.8 kg d−1; Mean±SD) were assigned to each treatment using a double 3 × 3 × 3 Latin Square Design. Compared with treatment C, the milk fat concentrations of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Conjugated linoleic acid; Dairy cow; Linoleic acid; Milk fatty acid. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000600315 |
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| Botaro,Bruno Garcia; Lima,Ygor Vinícius Real de; Cortinhas,Cristina Simões; Silva,Luís Felipe Prada e; Rennó,Francisco Palma; Santos,Marcos Veiga dos. |
| The objective of this study was to evaluate the effect of genetic polymorphism of kappa-casein, breed and seasonality on the physicochemical characteristics, composition and stability of milk in commercial dairy herds. A total of 879 milk and blood samples were collected from 603 Holstein and 276 Girolando cows, obtained during rainy and dry seasons. Milk samples were analyzed to determine the physicochemical characteristics, composition and ethanol stability, while blood samples were subjected to polymerase chain reaction to identify the kappa-casein genotype. The frequencies of genotypes AA, AB and BB of k-casein were respectively, 66.83, 31.84 and 1.33% for Holstein, and 71.38, 27.90 and 0.72% for the Girolando cows, respectively. The A allele was more... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Crioscopy; Dairy cow; Ethanol stability test; Genetic variant; Polymerase chain reaction; Titratable acidity. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009001200022 |
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| Morales,Álvaro; Grob,Daniela; Wittwer,Fernando; Müller,Ananda; Balocchi,Óscar; Pulido,Rubén. |
| ABSTRACT This study was conducted to evaluate the influence of management tools - daily pasture allowance and corn silage supplementation - during periods of forage shortage on the metabolism of dairy cows grazing low-mass pasture in temperate regions. Forty lactating Holstein cows were used during an experimental period of seventy days (April 15 to June 23, 2012). Blood metabolites and milk production were determined in fall-calving dairy cows grazing under two daily pasture allowances (PA) (moderate, 17 kg vs. high, 25 kg dry matter (DM)) and supplemented with corn silage (CS) (low, 4.5 kg vs. high, 9.0 kg DM). All cows received 3 kg DM of concentrate. Plasma concentrations of β-hydroxybutyrate (BHBA), non-esterified fatty acids (NEFA), and urea were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dairy cow; Grazing system; Metabolism; Restricted feeding; Supplementation. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016001100686 |
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| Amaral,Rafael Camargo do; Santos,Mateus Castilho; Daniel,João Luiz Pratti; Sá Neto,Adir de; Bispo,Álvaro Wosniak; Cabezas-Garcia,Edward Hernando; Bernardes,Thiago Fernandes; Nussio,Luiz Gustavo. |
| The objective of this study was to evaluate the effect of covering methods on the nutritive value of corn silage and performance of dairy cows. Whole-plant corn was harvested at 340 g/kg of dry matter (DM) and ensiled for 135 d in horizontal silos covered with one of the following methods: oxygen barrier film (45-µm thick) + white-on-black polyethylene film (200-µm thick) over the oxygen barrier film (OB+WB); white-on-black polyethylene film (200-µm thick) (WB); black polyethylene film (200-µm thick) (B); or recycled black polyethylene film (200-µm thick) covered with a layer of 10 cm of sugarcane bagasse (RB+SB). Nutrient composition, fermentation profile, and yeast and mold counts in edible silages were similar across treatments. Silage temperature... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dairy cow; Film protection; In vivo digestibility; Polyamide. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000900471 |
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| Lammoglia,Miguel Angel; Garcez,Nora; Cabrera,Amalia; López,Rubén Dario; Rentería,Iliana del Carmen Daniel; Rojas-Ronquillo,Rebeca. |
| ABSTRACT The objective of the study was to determine the effect of routine intramuscular injection of oxytocin to induce milk ejection on behavior assessed in terms of stress and rectal temperature in crossbred cows (Bos indicus × Bos taurus) in the tropics of Veracruz, México. Immediately after calving, cows were milked twice a day (n = 210) and randomly assigned to receive at each milking: 10 IU (0.5 mL) of oxytocin intramuscularly (n = 70); 0.5 mL of saline solution intramuscularly (n = 70); or no injection (n = 70). The following behavioral indicators of stress were observed: trampling, kicking, tail movement, failure to eat, jumping to avoid injection, vocalization, urination, and defecation. After milking, rectal temperature (RT) was measured.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Animal welfare; Dairy cow; Stress. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016000800478 |
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| Meyer,Paula Marques; Machado,Paulo Fernando; Coldebella,Arlei; Cassoli,Laerte Dagher; Coelho,Karyne Oliveira; Rodrigues,Paulo Henrique Mazza. |
| The objective of this study was to validate three different models for predicting milk urea nitrogen using field conditions, attempting to evaluate the nutritional adequacy diets for dairy cows and prediction of nitrogen excreted to the environment. Observations (4,749) from 855 cows were used. Milk yield, body weight (BW), days in milk and parity were recorded on the milk sampling days. Milk was sampled monthly, for analysis of milk urea nitrogen (MUN), fat, protein, lactose and total solids concentration and somatic cells count. Individual dry matter intake was estimated using the NRC (2001). The three models studied were derived from a first one to predict urinary nitrogen (UN). Model 1 was MUN = UN/12.54, model 2 was MUN = UN/17.6 and model 3 was MUN =... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dairy cow; Milk urea nitrogen; Nitrogen excretion; Protein utilization. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000500026 |
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| Registros recuperados: 46 | |
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