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Registros recuperados: 21 | |
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Delcroix, Jeremy; Gatesoupe, F.j.; Desbruyeres, Elisabeth; Huelvan, Christine; Le Delliou, Herve; Le Gall, Marie-madeleine; Quazuguel, Patrick; Mazurais, David; Zambonino-infante, Jose-luis. |
Protein hydrolysate is an essential component of dry starter diets for fish larvae, as promoting healthy development. Peptides are also suitable substrates for many intestinal microbes. Five experimental diets were compared to a control diet (CONT) supplemented with a commercial fish protein hydrolysate. Each diet contained one marine protein hydrolysate, which differed by the proportion of di- and tri-peptides, and by raw materials. Two diets (HYD4 and HYD5) stimulated larval growth compared with CONT. Two other diets (HYD1 and HYD2) yielded inferior growth. HYD1 was detrimental for survival, which was associated with an up-regulation of genes involved in inflammation and antioxidative responses, while the gene coding for osteocalcin was down-regulated in... |
Tipo: Text |
Palavras-chave: Compound diets; Denaturing gradient gel electrophoresis; Digestive enzymes; Gene expression; Gut maturation; Peptides. |
Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00197/30865/29268.pdf |
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Gisbert, Enric; Skalli, Ali; Fernandez, Ignacio; Kotzamanis, Yannis; Zambonino, Jose Luiz; Fabregat, Rogelio. |
In this study, we have evaluated the incorporation of two types of protein hydrolysates at 9 and 12% levels of inclusion, one from yeast (Saccharomyces cerevisiae, YPH) and another one from pig blood (PBPH), in microdiets for gilthead sea bream (Sparus aurora) larvae, and compared these results to a microdiet containing fish protein hydrolysate and another group only fed with enriched live prey (rotifers and Artemia). The trial consisted in substituting up to 75% (wt/wt) the enriched Artemia with the experimental microdiets from 15 to 40 days post-hatch, whereas larvae were exclusively fed on microdiets from 40 to 55 dph. Protein hydrolysates used in the present study were obtained from different raw materials (yeast, pig blood and fish protein... |
Tipo: Text |
Palavras-chave: Digestive enzymes; Larvae; Gilthead sea bream; Protein hydrolysate; Skeletal deformities; Sparus aurata. |
Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00085/19584/17467.pdf |
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Gamboa Delgado, Julián; Molina Poveda, César; Cahu, Chantal. |
A study was conducted to evaluate variations of digestive enzyme activities in Litopenaeus vannamei (Boone) reared in commercial ponds under semi-intensive conditions. Shrimp were collected at each body weight increase of 2 g. As the shrimp grew (2-12 g), significant increases in the activities of lipase and chymotrypsin were observed. The total protease activity decreased from 6 g onwards. Trypsin activity showed a peak at 6 g and amylase activity increased two-fold after 2 g. Additionally, the stomach contents were analysed microscopically for shrimp between 2 and 10 g. Plant matter contributed above 30% of the total stomach content in 6-, 8- and 10-g shrimp. Detritus represented 58% and 62% of the total stomach content in 2- and 4-g shrimp,... |
Tipo: Text |
Palavras-chave: Semi intensive culture; Stomach content; Digestive enzymes; Litopenaeus vannamei; Shrimp. |
Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-902.pdf |
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Castro-ruiz, Diana; Andree, Karl B.; Solovyev, Mikhail M.; Fernández-méndez, Christian; García-dávila, Carmen; Cahu, Chantal; Gisbert, Enric; Darias, Maria J.. |
Pseudoplatystoma punctifer is an Amazonian catfish highly appreciated for its high flesh quality, size, and commercial value. Its aquaculture is pursued to satisfy the demands of an increasing population in the region. However, knowledge of the nutritional needs during the early life stages is necessary for improving growth and reducing the incidence of cannibalism, factors that limit the success of its commercial farming. This study aimed at evaluating the influence of four diets containing different protein and lipid levels (30:15, 30:10, 45:15, or 45:10 in %) in the digestive physiology and performance of early juveniles. The results showed that the dietary protein:lipid as well as carbohydrate levels and ratios influenced differently the whole-body... |
Tipo: Text |
Palavras-chave: Neotropical fish; Aquaculture; Digestive enzymes; Gene expression; Nutrition; Juveniles; Histology; Development; Macronutrients; Diet. |
Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00678/79019/81420.pdf |
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Zambonino, Jose-luis; Cahu, Chantal. |
During these last 20 years, many studies have focussed on the development of the digestive tract in marine fish larvae. Most of the studies aimed at acquiring knowledge on the optimal form of dietary supply for different nutrients, in order to formulate a compound diet able to totally replace live preys in the fish larvae feeding sequence. Consequently, most of the studies aimed at describing the effect of dietary adaptation on digestive enzymes profile, the morphology of the main organs, while others aspects of the physiology of the larvae digestive tract (gut hormones, intestinal transport...) were poorly investigated. This review reports the more recent data on dietary modulation of digestive enzymes with a particular emphasis, when possible, on the... |
Tipo: Text |
Palavras-chave: Nuclear receptors; Digestive enzymes; Fatty acids; Vitamins; Dietary proteins and lipids; Fish larvae. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3045.pdf |
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Comoglio, Li; Gaxiola, G; Roque, A; Cuzon, Gerard; Amin, O. |
Juveniles of the white shrimp Litopenaeus vannamei were kept Without food for between 0 to 15 clays to evaluate the impact of starvation oil physiologic state (oxygen consumption, poststarvation refeeding, index, nitrogen excretion, and O:N ratio) and digestive enzymes activity. Physiologic changes were found after 6 days of fasting, and refeeding ability declined as a result. Nevertheless. the shrimp were able to Survive 16 days Without food. Starvation Caused metabolism to drop progressively toward a basal level (21 J (.) h(-1 .) g(-1)) and a decrease in the rate of ammonia excretion, because of the catabolism of amino acids front soluble protein in the hepatopancreas. This decrease led to an increase in digestive enzymes specific activity (U/mg... |
Tipo: Text |
Palavras-chave: Digestive enzymes; Litopenaeus vannamei; Metabolic rate; Shrimp; Starvation. |
Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/00000/10877/7911.pdf |
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Galgani, Francois; Ceccaldi, Hubert J.; Aquacop, Aquacop. |
In order to improve the formulation of diets fed to reared Penaeus vannamei, the effects of soya bean and fish meals on the growth, survival, and the characteristics of the hepatopancreas and digestive enzymes of the species were studied. Four soya bean meal concentrations (0, 5, 15, 40%), four fish meal concentrations (0, 5, 15, 30%), two forms of soya bean meal and three fish meals of different geographical origin were tested. Experimental conditions were as follows: ten individuals were placed in each tank (0.5 m2 ) and two tanks were subjected to each treatment. Food was given twice a day (08-09.00 h and 19.00 h) at 10% of the initial weight each day. For biochemical analyses, nine individuals per treatment were used for the determination of the... |
Tipo: Text |
Palavras-chave: Aquaculture; Nutrition; Pénéidés; Soja; Farine de poisson; Enzymes; Enzymes digestives.; Aquaculture; Nutrition; Penaeidae; Soya bean; Fish meal; Digestive enzymes. |
Ano: 1988 |
URL: http://archimer.ifremer.fr/doc/00135/24597/22626.pdf |
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Buchet, Vincent; Zambonino, Jose-luis; Cahu, Chantal. |
Red drum larvae were fed from day 11 to day 24 post-hatch, with Artemia (LP group) or two isoproteinic-formulated diets with varying lipid levels, 15% (L15 group) and 30% (L30 group). The lipid fraction was composed of a mixture of cod liver oil and soy lecithin. Larvae fed Artemia exhibited the greatest length, L30 diet induced a larvae length significantly greater than that obtained with L15 diet (P < 0,05). No difference was observed in larvae survival among the three dietary groups. The increase in dietary lipid concentration led to an increase in amylase and trypsin secretion levels in 24-day-old larvae. The pancreatic secretion level measured in L30 group was close to that of LP group, suggesting that the maturation of the pancreatic functions was... |
Tipo: Text |
Palavras-chave: Gut maturation; Digestive enzymes; Compound diet; Lipid; Larvae; Red drum. |
Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-503.pdf |
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Aragon-axomulco, Heron; Chiappa-carrara, Xavier; Soto, Luis; Cuzon, Gerard; Arena, Leticia; Maldonado, Carlos; Cardenas, Rene; Gaxiola, Gabriela. |
In view of the relationship between shifts in diet composition and the activity of digestive enzymes in penaeid shrimp, the present study focused on the analysis of digestive trypsin and a-amylase activities of wild Farfantepenaeus. duorarum (Burkenroad, 1939) juveniles and their changes in phenotypic expression, during the molt cycle as endogenous factor and their changes due to different feeding regimes (exogenous factor) in relation with delta C-13 and delta N-15 isotopic signature as an index of food assimilation induced by the seasonal availability of food items in the nursery area. Wild juveniles of F. duorarum were captured from April 2007 to February 2008, in the Celestun coastal lagoon, Yucatan, Mexico. Samplings were carried out considering all... |
Tipo: Text |
Palavras-chave: Coastal lagoons; Digestive enzymes; Farfantepenaeus duorarum; Feeding habits; Pink shrimp. |
Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00078/18880/16487.pdf |
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Cahu, Chantal; Zambonino, Jose-luis. |
The production of fish larvae by hatchery shows an intensive increase for several species, but the specific nutritional requirements of larvae were poorly known until some years. We have conducted several experiments aimed to understand the specificities in larvae digestion, and to formulate a compound diet taking into account the differences compared to juveniles. The chosen species was the European sea bass, Dicentrarchus labrax, and the data were validated on different species. Larvae were fed live preys or experimental compound diet, especially formulated for the experiment. It appeared that pancreatic digestive enzymes, such as trypsin and amylase could be assayed in early ontogenesis, before mouth opening, and increase dramatically during the first... |
Tipo: Text |
Palavras-chave: Peptides; Phospholipid; Digestive enzymes; Nutrition; Larvae; Dicentrarchus labrax. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3332.pdf |
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Gatesoupe, Joel; Huelvan, Christine; Le Bayon, Nicolas; Severe, Armelle; Aasen, Inga Marie; Degnes, Kristin F.; Mazurais, David; Panserat, Stephane; Zambonino, Jose-luis; Kaushik, Sadasivam. |
The aim of this work was to investigate the catabolic process of three kinds of dietary carbohydrates in the gut of sea bass juveniles, with the possible contribution of the intestinal microbiota to the nutrition of the host, and the subsequent effects on intermediary metabolism. A first diet contained waxy maize (99% amylopectin), a highly digestible form of starch. A second diet was less quickly digestible due to its high amylose content of resistant starch. Two other diets contained fibre instead of starch, either only cellulose as control, or also other non-starch polysaccharides brought by lupin meal. The effect of the diets on the host confirmed previous results, with the stimulation of glucose storage in the liver in sea bass fed the starchy diets,... |
Tipo: Text |
Palavras-chave: Digestive enzymes; Glucose metabolism; Intestinal microbiota; Viscerosomatic index; Gene expression; Metabolite analyses; Vegetable feedstuffs. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00166/27763/25944.pdf |
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Silva, Flavia; Nicoli, Jacques R.; Zambonino, Jose-luis; Le Gall, Marie-madeleine; Kaushik, Sadasivam; Gatesoupe, François-joel. |
The sustainable composition of diets of high nutritional value is of the utmost importance for intensive aquaculture. Digestion and absorption of nutrients depends on the activity of the digestive enzymes, in particular those located in the brush border membrane of enterocytes, which are responsible for the final stages of breaking down and absorption of nutrients. In the present study, the substitution of fish meal by lupin or rapeseed meal in the diet was evaluated on gilthead sea bream (Sparus aurata) and goldfish (Carassius auratus). The objectives were to compare the activities of intestinal brush border enzymes in both species fed the control and experimental diets. When gilthead sea bream were fed the vegetable diets, significantly lower activities... |
Tipo: Text |
Palavras-chave: Sparus aurata; Carassius auratus; Fish meal replacement; Brush border membrane; Digestive enzymes; Alkaline phosphatase; Aminopeptidase N; Maltase; Glutamyl transpeptidase. |
Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00009/11975/8679.pdf |
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Hamza, N; Mhetli, M; Ben Khemis, I; Cahu, Chantal; Kestemont, P. |
This study was carried out to evaluate the effects of dietary phospholipid on the development and rearing performance of pikeperch (Sander lucioperca) larvae. From day 10 post-hatching, fish larvae were weaned onto three isoproteic and isolipidic formulated diets with different phospholipid (PL) levels: 1.4 (PL1), 4.7 (PL5) and 9.5% (PL9) of dry matter, as soybean lecithin. Neutral lipid (NL) with inversed gradient was incorporated in diets. Survival, growth and deformities were monitored until day 34 post-hatching, as well as intestinal enzyme activities, leucine alanine peptidase (leu-ala), aminopeptidase N (AN) and alkaline phosphatase (AP), which were used as indicators of digestive tract maturation. This study showed that PL supplementation... |
Tipo: Text |
Palavras-chave: Pikeperch; Phospholipids; Larval development; Fatty acid; Digestive enzymes. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4149.pdf |
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Zambonino, Jose-luis; Mazurais, David; Dubuc, Alexia; Queau, Pierre; Vanderplancke, Gwenaelle; Servili, Arianna; Cahu, Chantal; Le Bayon, Nicolas; Huelvan, Christine; Claireaux, Guy. |
Ocean warming, eutrophication and consequent decrease in oxygen lead to smaller average fish size. Although such responses are well-known in an evolutionary context, involving multiple generations, it appears to be incompatible with current rapid environmental change. Rather, phenotypic plasticity could provide a means for marine fish to cope with rapid environmental changes. However, little is known about the mechanisms underlying plastic responses to environmental conditions that favour small phenotypes. Our aim was to investigate how and why European sea bass that had experienced a short episode of moderate hypoxia during their larval stage subsequently exhibited a growth depression at the juvenile stage compared to the control group. We examined... |
Tipo: Text |
Palavras-chave: Climate change; Digestive enzymes; Dicentrarchus labrax; Growth; Hypoxia; Respirometry. |
Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00373/48407/48635.pdf |
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Tovar, Dariel. |
The interest in probiotics for aquaculture is increasing rapidly. In the present work we evaluated the potential of some yeast to induce the larvae gut maturation in sea bass larvae Dicentrarchus labrax. The production of polyamines putrescine, spermidine and spermine was quantified in ten yeast strains using high performance liquid chromatography (HPLC). Afterwards, fluorescently labelled yeast adhesion to the intestine of sea bass larvae and the spotted sand bass (Paralabrax maculatofasciatus) was evaluated by fluorescent microscopy. Two of them, CBS 8339 strain (Debaryomyces hansenii) and X2180 strain (Saccharomyces cerevisiae) produce the polyamines of our interest. The same strains showed the best adhering capabilities to the sea bass and spotted sand... |
Tipo: Text |
Palavras-chave: Ontogeny; Digestive enzymes; Fish larvae; Polyamines; Probiotics; Ontogénèse; Enzymes digestives; Larves de poisson; Polyamines; Probiotiques. |
Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/these-103.pdf |
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Routman,KS; Yoshida,L; Frizzas de Lima,AC; Macari,M; Pizauro Jr.,JM. |
Thirty-two Hubbard broilers were raised under commercial husbandry to evaluate the effect of temperature exposure (26 and 35ºC) on the activity of digestive enzymes at different ages (23 and 46 days) and different diet energy levels (2,900 and 3,200 kcal ME/kg). Data were analyzed in a 2x2x2 factorial arrangement (energy/temperature/age) in a completely randomized design. Animals were slaughtered after four hours of heat exposure for sampling of an intestinal segment, to extract alkaline phosphatase, and pancreas, to extract amylase, lipase and trypsin. The activity of intestinal alkaline phosphatase was influenced by energy level and animal age. Energy level of diet and environmental temperature interfered on the activity of pancreatic amylase. Lipase... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broilers; Digestive enzymes; Thermal stress. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2003000100003 |
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Registros recuperados: 21 | |
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