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	Provedor de dados Arq. Bras. Med. Vet. Zootec. (1) BJID (1) Autor Bernardo,F. (1) Carvalho,Darlene (1) Franck,Jacqueline (1) Martins,C. (1) Oliveira,M. (1) Pedrosa,William (1) Piarroux,Renaud (1) Souza,Guenael Freire de (1) Vieira-Pinto,M. (1) Mais... Palavra-chave ELFA (2) ECLIA (1) FISH (1) Immunoassay (1) Salmonella (1) Swine (1) Toxoplasma (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/other (1) Ano 2012 (1) 2007 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Rapid detection of Salmonella sp. in pork samples using fluorescent in situ hybridization: a comparison with VIDAS®-SLM system and ISO 6579 cultural method Arq. Bras. Med. Vet. Zootec. Vieira-Pinto,M.; Oliveira,M.; Bernardo,F.; Martins,C.. This study reports the use of the fluorescent in situ hybridization (FISH) with Sal3 probe for Salmonella detection in swine carcasses inner surface (swab); and in the correspondent samples of ileum, ileocolic, and mandibular lymph nodes; and tonsils, after dilution (1:10) in buffered peptone water and a pre-enrichment step (37(0)C, 18h). In order to evaluate the efficiency of FISH, 235 naturally contaminated samples were simultaneously tested by the cultural method (ISO 6579) and by the Vitek Immuno Diagnostic Assay System (VIDAS®) - Salmonella (SLM) system. The cultural method identified 39 positive samples. From these, VIDAS®- SLM only detected 23. FISH identified 115 positive samples. This difference was highly significant (P<0.001). From positive... Tipo: Info:eu-repo/semantics/article Palavras-chave: Swine; Salmonella; FISH; ELFA. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352007000600006 Analytical validation of anti-toxoplasma IgG immunoassays BJID Souza,Guenael Freire de; Carvalho,Darlene; Pedrosa,William; Franck,Jacqueline; Piarroux,Renaud. There are often discrepancies when using different methods to measure anti-Toxoplasma gondii IgG levels in patient samples. The diagnostic performance of a chemiluminescent immunoassay (CLIA) and an enzyme-linked fluorescent assay (ELFA) used as confirmatory tests for samples identified as positive or equivocal by an electrochemiluminescent immunoassay (ECLIA) were examined. Cut-off values were those stated by the manufacturer, and Western blot was used to confirm the results of all methods. All samples identified as positive by ECLIA (n = 93) were confirmed as positive by Western blot, as were 14 of the 28 samples identified as equivocal. When these 121 samples were retested, the sensitivities of CLIA and ELFA were 64.4% and 73.8%, respectively. Both... Tipo: Info:eu-repo/semantics/other Palavras-chave: Toxoplasma; Immunoassay; ECLIA; ELFA. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600012 Registros recuperados: 2 Primeira ... 1 ... Última

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