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The dispersal of nitrogen-fixing Enterobacteriaceae from sewage into the waters and sediments of Morecambe Bay, UK. ArchiMer
Jones, K; Betaieb, M.
The sediments of Morecambe Bay contain large numbers of salinity-tolerant, nitrogen-fixing Enterobacteriaceae (NFEs) mainly Klebsiella pneumoniae ) which appear to be derived from sewage. Sewage effluents, regardless as to whether they have received primary or secondary treatment, contain huge numbers of NFEs with salinity-tolerant and freshwater strains present in roughly equal quantities. The bacteria are discharged into the rivers and estuaries and carried out to sea. Once in the sea the salinity-tolerant strains retain their viability better than the freshwater strains so that the incoming tide, which doses the sediments, contains mainly salinity-tolerant NFEs. It is probable that similar processes occur all round the U.K. coast, as inshore coastal...
Tipo: Text Palavras-chave: Klebsiella pneumoniae; Enterobacteriaceae; Salinity tolerance; Microbial contamination; Pathogenic bacteria; Bacteria; Sewage; Nitrogen fixation; Sediment pollution; Sea water.
Ano: 1984 URL: http://archimer.ifremer.fr/doc/1984/acte-1003.pdf
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Development of a real-time PCR method coupled with a selective pre-enrichment step for quantification of Morganella morganii and Morganella psychrotolerans in fish products ArchiMer
Podeur, Gaetan; Dalgaard, Paw; Leroi, Francoise; Prevost, Herve; Ernborg, Jette; Martinussen, Jan; Hansen, Lars Hestbjerg; Filet, Marie-france.
Histamine fish poisoning is common and due to toxic concentrations of histamine often produced by Gram-negative bacteria in fin-fish products with a high content of the free amino acid histidine. The genus Morganella includes two species previously reported to cause incidents of histamine fish poisoning. Morganella morganii and Morganella psychrotolerans are both strong producer of histamine. However, little is known about the occurrence and critical stages for fish contamination with these bacteria. To elucidate contamination routes of Morganella, specific real-time quantitative PCR (RTi qPCR) methods for quantification of M. morganii and M. psychrotolerans have been developed. Selective primers amplified a 110 bp region of the vasD gene for M....
Tipo: Text Palavras-chave: Histamine-producing bacteria; Tuna; Selective medium; Enterobacteriaceae; Galactokinase; Type VI secretion system.
Ano: 2015 URL: https://archimer.ifremer.fr/doc/00254/36551/35096.pdf
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Environmental parameters and antimicrobial susceptibility of enterobacteriaceae isolated from estuarine waters of São Vicente, São Paulo state, Brazil J. Venom. Anim. Toxins incl. Trop. Dis.
Lourenço,N. G. G. S.; Takahashi,C. K.; Lopes,T. F.; Lopes,C. A. M..
Diseases transmitted by water consists a serious public health problem and enterobacteria are the main group of microorganisms responsible for outbreaks in humans. Such pathogenic bacteria proliferate in water polluted by domestic and industrial sewage and reach the population through seawater contact. The aim of the present work was to study environmental parameters as well as to identify Enterobacteriaceae species and their antimicrobial susceptibility in water samples collected from the estuarine area of São Vicente city (São Paulo State, Brazil). Strains were identified by using traditional biochemical tests described in literature and antimicrobial susceptibility tests were carried out using the disk diffusion method. Out of 26 samples, Escherichia...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Estuarine water; Environmental parameters; Enterobacteriaceae; Antimicrobial susceptibility.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200006
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Inhibición de los contaminantes del tempeh de soya OceanDocs
Mejías, E.; Delgado, R.; Silveira, I.; Lafargue, D.; Cárdenas, E..
Se evaluó el efecto de diferentes especias o condimentos sobre la calidad microbiológica del tempeh y la posible acción inhibidora de sus contaminantes. El tempeh se obtuvo a partir de frijol de soya el cual se sometió a los procesos de cocción, escurrimiento y fermentación. A continuación se procedió a incorporar los condimentos ensayados en dos proporciones por separado a la soya fresca antes de su inoculación con la cepa R. oligosporus, se mezclaron bien y se sometieron al proceso de fermentación, el cual tuvo lugar en bandejas de poliestireno, incubadas a 30 ± 2 ºC por un período de 18 a 20 h hasta obtener un producto con las características típicas del tempeh. De éste se tomaron muestras para determinar contenido de humedad, conteo total de viables y...
Tipo: Journal Contribution Palavras-chave: Biological contamination; Chemical activity; Tempeh; Bacterial contamination; Enterobacteriaceae; Inhibition; Garlic; Anions; Spices.
Ano: 2008 URL: http://hdl.handle.net/1834/5010
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บทบาทของเชื้อแบคทีเรียในทางเดินอาหารของแมลงวันผลไม้ (Diptera: Tephritidae) Thai Agricultural
Narit Thaochan.
Tipo: PhysicalObject Palavras-chave: Fruit flies; Diptera; Tephritidae; Bactrocera dorsalis; Enterobacteriaceae; Symbiosis; Parasitism; Gut bacteria; Biodiversity; Behaviour; Growth; แมลงวันผลไม้; เชื้อแบคทีเรีย; ภาวะแบบพึ่งพาอาศัยกัน; ภาวะที่เป็นโทษต่อกัน; ทางเดินอาหาร; ความหลากหลายทางชนิด; กระบวนการเจริญเติบโต; การแสดงพฤติกรรม; การควบคุมประชากร; การควบคุมแมลงวันผลไม้.
Ano: 2013 URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5765
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Diversidad de ß-lactamasas en aislamientos clínicos de enterobacterias ABCL
Casabonne,Cecilia; Pérez,Jorgelina; Balagué,Claudia; Fernández,Luisa.
La rápida emergencia de resistencia a antimicrobianos debida a la presencia de b-lactamasas de espectro extendido (BLEE) tiene un impacto significativo en la salud pública. Las BLEEs son enzimas producidas por bacilos gramnegativos y confieren resistencia a las penicilinas, a todas las cefalosporinas y al aztreonam, pero no a los carbapenemes ni a las cefamicinas y la mayoría son inhibidas por el ácido clavulánico. El objetivo de este trabajo fue evaluar la resistencia a antibióticos b-lactámicos en aislamientos de Klebsiella pneumoniae, Escherichia coli y Proteus mirabilis y caracterizar las b-lactamasas responsables de dicha resistencia. Se analizaron 2.030 aislamientos (362 Klebsiella pneumoniae, 1.250 Escherichia coli y 175 Proteus mirabilis)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: SS-lactamasas de espectro extendido; Enterobacteriaceae; Reacción en cadena de la polimerasa.
Ano: 2012 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0325-29572012000300009
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Produção de ácido indol acético por microorganismos solubilizadores de fosfato e sua interação com fungos micorrízicos arbusculares - DOI: 10.4025/actascibiolsci.v29i3.492 Biological Sciences
Souchie, Edson Luis; cefet- Goiás; Azcón, Rosario; CSIC; Barea, Jose Miguel; CSIC; Saggin-Júnior, Orivaldo José; EMBRAPA-RJ; Silva, Eliane Maria Ribeiro da; Embrapa.
Avaliou-se o potencial de produção de ácido indol acético (AIA) in vitro por cinco isolados de fungo solubilizador de fosfato (FSF) do gênero Aspergillus sp. e quatro isolados de bactéria solubilizadora de fosfato (BSF) da família Enterobacteriaceae. Os isolados de bactéria e fungo foram incubados em meio líquido a 28ºC, por dois e quatro dias, respectivamente. Além disso, dois isolados de BSF da família Enterobacteriaceae (BSF 8 e BSF 56) foram avaliados quanto à capacidade de estimular ou inibir a germinação de esporos e o desenvolvimento micelial de duas espécies de fungos micorrízicos arbusculares (FMAs) in vitro. Foram instalados dois ensaios em placas de Petri, contendo agar-água (0,8%); um, utilizando o FMA Gigaspora margarita e o outro, com o FMA...
Palavras-chave: 2.12.00.00-9 Microbiologia sinergismo; Enterobacteriaceae; Regulador vegetal; Aspergillus sp.; Gigaspora margarita; Scutellospora sp.
Ano: 2007 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/492
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Prevalência de enterobactérias em Bothrops jararaca no Estado de São Paulo: levantamento microbiológico e padrões de resistência antimicrobiana - DOI: 10.4025/actascibiolsci.v30i3.536 Biological Sciences
Bastos, Henrique Marçal; Lopes, Luiz Fernando Larangeira; USP - São Paulo; Gattamorta, Marco Aurélio; UNICAMP - Campinas; Matushima, Eliana Reiko; USP - São Paulo.
Existem poucos levantamentos microbiológicos em répteis no Brasil. O estudo aqui descrito enfoca uma espécie de serpente de grande interesse médico e serve como base para outros estudos e comparações. As amostras foram colhidas diretamente do cólon de jararacas adultas e saudáveis. O material foi semeado em ágar MacConkey (Difco®) e ágar XLT4 (Difco®). Os isolados foram identificados por meio do Sistema API 20E de Identificação Bioquímica (Galerias API-BioMériux®). Os isolados de Salmonella sp. foram submetidos à sorotipagem. Por fim, as colônias foram submetidas ao teste de sensibilidade antimicrobiana. Foram obtidos vários gêneros da família Enterobacteriaceae (Citrobacter, Enterobacter, Escherichia, Klebsiella, Kluyvera, Morganella, Proteus,...
Palavras-chave: 2.00.00.00-6 Ciências Biológicas Bothrops jararaca; Snake; Reptile; Intestinal microbiota; Enterobacteriaceae; Salmonella. Bothrops jararaca; Snake; Reptile; Intestinal microbiota; Enterobacteriaceae; Salmonella.
Ano: 2008 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/536
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Communities of P-solubilizing bacteria, fungi and arbuscular mycorrhizal fungi in grass pasture and secondary forest of Paraty, RJ - Brazil Anais da ABC (AABC)
Souchie,Edson L.; Saggin-Júnior,Orivaldo J.; Silva,Eliane M.R.; Campello,Eduardo F.C.; Azcón,Rosario; Barea,Jose M..
Communities of P-solubilizing bacteria, fungi and arbuscular mycorrhizal fungi, were evaluated in two different ecosystems. Samplings taken from two areas of Atlantic forest, in Paraty - RJ, Brazil, one with a secondary forest and the other with a grass pasture were studied. Four growth media: GL (glucose and yeast extract), GES (glucose, soil extract, KNO3, CaCl2, MgSO4, NaCl, FeEDTA and micronutrients solution), GAGES (glucose, soil extract, arabinose, glycerol, CaCl2, MgSO4 and NaCl) and GELP (glucose, soil extract, yeast extract, peptone, CaCl2, MgSO4 and NaCl) were evaluated for the isolation of P-solubilizing microorganisms. The identification of P-solubilizing bacteria was based on 16 S rDNA sequence analysis, while the identification of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phosphate; Enterobacteriaceae; Atlantic Forest; Glomeromycota; Soil microorganisms.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000100016
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Detecção e avaliação do perfil de sensibilidade antimicrobiana de enterobactérias isoladas de periquitos cara-suja (Pyrrhura griseipectus) em cativeiro Arq. Bras. Med. Vet. Zootec.
Machado,D.N.; Lopes,E.S.; Albuquerque,Á.H.; Bezerra,W.G.A.; Horn,R.V.; Lima,S.V.G.; Siqueira,R.A.S.; Beleza,A.J.F.; Oliveira,F.R.; Cardoso,W.M.; Teixeira,R.S.C..
ABSTRACT This study was conducted to detect enterobacteria in captive Grey-breasted parakeet from a commercial breeding located in the state of Ceara. 25 individual cloacal swabs were collected and this material was subjected to microbiological processing to obtain the bacterial isolation, identification, and evaluation of the sensitivity profile to antimicrobials. The bacterial isolation in ten samples was determined, belonging to the genera Enterobacter, Hafnia, Serratia, Escherichia, Pantoea and Klebsiella, in which these last three corresponded to the bacteria most isolated, with respective percentages of 28.6%, 21.4%, and 21.4%. As for the sensitivity profile, antibiotics to which the bacteria showed more resistance were trimethoprim-sulfamethoxazole...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Isolamento; Enterobacteriaceae; Psitacídeos; Resistência.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000601732
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Investigação de Salmonella spp. em Psittaciformes exóticos e nativos mantidos em cativeiro na região central do Rio Grande do Sul Arq. Bras. Med. Vet. Zootec.
Murer,L.; Didoné,S.R.; Freitas,A.B.B.; Lovato,M..
RESUMO As doenças infecciosas são reconhecidamente causadoras de declínios populacionais de animais silvestres e algumas delas podem representar ameaça à saúde pública. O presente estudo objetivou investigar a ocorrência de Salmonella spp. em Psittaciformes exóticos e nativos mantidos em cativeiro na região central do Rio Grande do Sul, além de comparar os resultados obtidos por meio do método bacteriológico convencional e da reação em cadeia da polimerase (PCR), mediante a utilização de material fecal do ambiente, evitando-se o estresse da contenção. Durante os meses de agosto/2016 e setembro/2016 foram coletadas amostras de fezes frescas de 90 gaiolas em dois criatórios, representando 180 aves. Com as duas técnicas empregadas nas análises, as amostras...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aves silvestres; Psitacídeos; Patógenos intestinais; Enterobacteriaceae; Zoonoses.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000300815
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Influence of the cleaning system of conveyor belts on microbiological quality of poultry meat Arq. Bras. Med. Vet. Zootec.
Julião,T.A.; Cortezi,A.M.; Rossi,G.A.M.; D’Abreu,L.F.; Balieiro,J.C.C.; Vidal,A.M.C..
ABSTRACT This study focused on assessing the microbiology of conveyor belts surfaces, cleaned or not with pressurized water, and verification of the influence of this process on the microbiological quality of poultry meat. A reduction on mesophilic, psychrotrophic and Enterobacteriaceae counts (P<0.05) on dry conveyor belts compared to wet ones was observed. For the chicken leg (consisting of drumstick and thigh) samples, no statistically significant differences were detected on counts of indicators of microorganisms on poultry legs (composed by drumstick and thigh). For poultry meat conducted on wet or dry conveyor belts, 99% and 86%, were positive for Listeria spp, respectively. Only one sample of chicken leg was positive for presence of L....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterobacteriaceae; Listeria monocytogenes; Food microbiology; Staphylococcus spp..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401325
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Isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, Helixpomatia and Cornu aspersum (Gastropoda: Pulmonata) Biol. Res.
CHARRIER,MAR YVONNE; FONTY,GERARD; GAILLARD-MARTINIE,BRIGITTE; AINOUCHE,KADER; ANDANT,GERARD.
The intestinal microbiota of the edible snails Cornu aspersum fSyn: H. aspersa), and Helix pomatia were investigated by culture-based methods, 16S rRNA sequence analyses and phenotypic characterisations. The study was carried out on aestivating snails and two populations of H. pomatia were considered. The cultivable bacteria dominated in the distal part of the intestine, with up to 5.10(9) CFU g -1, but the Swedish H. pomatia appeared significantly less colonised, suggesting a higher sensitivity of its microbiota to climatic change. All the strains, but one, shared &#8805; 97% sequence identity with reference strains. They were arranged into two taxa: the Gamma Proteobacteria with Buttiauxella, Citrobacter, Enterobacter, Kluyvera, Obesumbacterium,...
Tipo: Journal article Palavras-chave: Clostridium; Edible snails; Enterobacteriaceae; Enterococci; Gut microbiota; Lactococci.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500010
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Occurrence of extended-spectrum beta-lactamases in Enterobacteriaceae isolated from hospitalized patients in Curitiba, southern Brazil BJID
Nogueira,Keite da Silva; Higuti,Ilma Hiroko; Nascimento,Agnaldo José do; Terasawa,Larissa Bail; Oliveira,Simone de; Matos,Adriana Pereira; Souza,Helena Aguilar Peres Homem de Mello de; Cogo,Laura Lúcia; Dalla Costa,Libera Maria.
Production of extended-spectrum beta-lactamases (ESBL) by enterobacteria is an important resistance mechanism against antimicrobial beta-lactamics. We tested 498 bacterial strains isolated from two tertiary-care teaching hospitals for ESBL production, using screening breakpoints for aztreonam and third generation cephalosporins, according to CLSI recommendations. Among these isolates, 155 were positive for the ESBL screening test, and 121 (78%) were confirmed by the clavulanic acid combination disk method. We found a high frequency of ESBL (24%) among Enterobacteriaceae, with a frequency of 57.4% for Klebsiella pneumoniae, 21.4% for Klebsiella oxytoca, and 7.2% for E. coli. In other members of Enterobacteriaceae, non-Klebsiella and non-E. coli, the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: ESBL; Beta-lactamases; Enterobacteriaceae.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000600006
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Evaluation of phenotypic tests to detect carbapenem-resistant Enterobacteriaceae in colonized patients hospitalized in intensive care units BJID
Perez,Leandro Reus Rodrigues; Rodrigues,Diógenes; Dias,Cícero Gomes.
In this study, we aimed to evaluate the performance of different phenotypic tests to detect carbapenem-resistant Enterobacteriaceae. Three different phenotypic methods were evaluated: (1) combined-disk test of meropenem plus phenylboronic acid or EDTA reading after 24 h and 48 h; (2) selective/chromogenic read after 24 h and after 48 h; and (3) overnight selective enrichment broth containing 10 µg ertapenem disk followed by culture on MacConkey agar. A positive result in at least one of the methods was submitted to PCR for blaNDM-1, blaOXA-48, blaKPC, blaSPM-1, blaIMP, and blaGES detection. Carbapenem-resistant Enterobacteriaceae was detected in 31 (30.4%) of 102 rectal swabs evaluated. All isolates showed to be KPC-2-producing organisms. Results showed...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Carbapenems; Enterobacteriaceae; KPC; Surveillance.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000400436
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Cefepime versus extended spectrum β-lactamase-producing Enterobacteriaceae BJID
Nogueira,Keite da Silva; Daur,Alessandra Vale; Reason,Iara Taborda de Messias; Gales,Ana Cristina; Costa,Libera Maria Dalla.
The objective of this study was to evaluate the susceptibility to cefepime of a large group of ESBL- producing enterobacteria recently isolated in a Brazilian teaching hospital . The study included 280 strains of ESBL-producing enterobacteria, isolated between 2005 and 2008. The presence of the genes blaCTX-M, blaTEM and blaSHV was determined by PCR and confirmed by nucleotide sequencing. Susceptibility testing for cefepime was performed by disc-diffusion, agar dilution method and E-test®. Among the isolates, 34 (12.1%) presented a cefepime inhibition zone &gt; 21 and MIC < 8 mg/L by agar dilution and E-strip methods. The use of cefepime for the treatment of infections caused by ESBL-producing bacteria has been controversial. Some studies of PD/PK...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterobacteriaceae; Beta-lactamases; Cephalosporin resistance.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200014
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Enterobacteriaceae resistant to third generation cephalosporins upon hospital admission: risk factors and clinical outcomes BJID
Oliveira,Mirian Cristina; Oliveira,Clara Rodrigues Alves; Gonçalves,Karine Valéria; Santos,Marciléa Silva; Tardelli,Amanda Cristina Silva; Nobre Jr.,Vandack Alencar.
Objectives: Evaluate risk factors and clinical outcomes of infections caused by Enterobacteriaceae resistant to third-generation cephalosporins present in samples collected upon hospital admission.Methods: Risk factors were evaluated using a 1:2 ratio case-control study. Influence of resistance on the appropriateness of antibiotic therapy, length of stay, and hospital mortality were prospectively evaluated. Characteristics independently associated with the presence of resistant enterobacteria were assessed by logistic regression.Results: Enterobacteria resistant to third-generation cephalosporins were quite common (26.0%). Male gender (OR: 2.66; 95% CI, 1.17-5.06; p= 0.019), invasive prosthesis (OR: 3.79; 95% CI, 1.29-11.08; p= 0.015), previous use of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enterobacteriaceae; Third generation cephalosporins; Risk factors; Antibiotic resistance.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000300239
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Emergence of colistin resistance in the largest university hospital complex of São Paulo, Brazil, over five years BJID
Rossi,Flávia; Girardello,Raquel; Cury,Ana Paula; Di Gioia,Thais Sabato Romano; Almeida Jr,João Nóbrega de; Duarte,Alberto José da Silva.
Abstract Colistin resistance involving Gram-negative bacilli infections is a challenge for health institutions around of the world. Carbapenem-resistance among these isolates makes colistin the last therapeutic option for this treatment. Colistin resistance among Enterobacteriaceae, Acinetobacter spp., and Pseudomonas spp. was evaluated between 2010 and 2014 years, at Hospital das Clínicas, São Paulo, Brazil. Over five years 1346 (4.0%) colistin resistant Gram-negative bacilli were evaluated. Enterobacteriaceae was the most frequent (86.1%) pathogen isolated, followed by Acinetobacter spp. (7.6%), and Pseudomonas spp. (6.3%). By temporal analysis there was a trend for an increase of colistin resistance among Enterobacteriaceae, but not among...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Colistin-resistant; Polymyxin B-resistant; Enterobacteriaceae; Brazil.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000100098
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Emergence of Klebsiella pneumoniae-producing KPC-2 carbapenemase in Paraíba, Northeastern Brazil BJID
Fehlberg,Lorena C.C.; Carvalho,Albalucia M.C.; Campana,Eloiza H.; Gontijo-Filho,Paulo P.; Gales,Ana C..
The emergence of KPC-2 producing K. pneumoniae in hospitalized patients at the intensive care unit (ICU) of a teaching hospital located in the city of João Pessoa, Paraíba, Brazil, is reported. Seven carbapenem-resistant K. pneumoniae recovered from different body sites of infection were analyzed. Most isolates showed a multidrug-resistance phenotype. Genotypic analysis demonstrated the presence of two genotypes, with the predominance of genotype A, which belongs to ST 437. These isolates also carry the encoding genes of five other betalactamases.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Beta-lactam resistance; Modified Hodge test; Carbapenem resistance; Enterobacteriaceae.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600013
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Molecular epidemiology of CTX-M producing Enterobacteriaceae isolated from bloodstream infections in Rio de Janeiro, Brazil: emergence of CTX-M-15 BJID
Seki,Liliane Miyuki; Pereira,Polyana Silva; Conceição,Magda de Souza; Souza,Maria José; Marques,Elizabeth Andrade; Carballido,Jupira Miron; Carvalho,Maria Elisabeth Serqueira de; Assef,Ana Paula D'Alincourt Carvalho; Asensi,Marise Dutra.
OBJECTIVE: The present studywas designed to evaluate the molecular epidemiology of CTX-M producing Klebsiella pneumoniae, Enterobacter cloacae and Escherichia coli isolated from bloodstream infections at tertiary care hospitals in the State of Rio de Janeiro, Brazil. MATERIAL AND METHODS: A total of 231 nonduplicate Enterobacteriaceae were isolated from five Brazilian hospitals between September 2007 and September 2008. The antimicrobial susceptibility testing was performed by disk diffusion method according to the Clinical Laboratory Standard Institute. Isolates showing resistance to third-generation cephalosporins were screened for ESBL activity by the double-disk synergy test. The presence of blaCTX-M , blaCTX-M-15 and blaKPC genes was determined by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bloodstream infections; Enterobacteriaceae; CTX-M-15; PFGE; KPC; Brazil.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000600003
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