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Hernández,Christian A; Sandoval,Norma; Mallerman,Julieta; García-Pérez,José Antonio; Farnet,Anne-Marie; Perraud-Gaime,Isabelle; Alarcón,Enrique. |
Background Ethanol has been pointed out as a laccase inducer. However, there are controversial reports about its efficiency with some fungi. In this study, we hypothesized that ethanol laccase induced in Pycnoporus sanguineus depends on nitrogen nutriment conditions. To prove this, we assessed laccase production in submerged cultures of P. sanguineus, with different nitrogen concentrations and with, or without ethanol added in a factorial designed experiment. Results In order to analyze the effects of factors on the response variables, a factorial ANOVA, and response-surface models were performed. It was found that the nitrogen source was the main factor that affected laccase production in P. sanguineus. The treatments with yeast extract (2 g/L) and... |
Tipo: Journal article |
Palavras-chave: Enzyme production; Fungal metabolism; Phenoloxidases; Urea; Yeast extract. |
Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000400012 |
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Souza,Paula Monteiro de; Bittencourt,Mona Lisa de Assis; Caprara,Carolina Canielles; Freitas,Marcela de; Almeida,Renata Paula Coppini de; Silveira,Dâmaris; Fonseca,Yris Maria; Ferreira Filho,Edivaldo Ximenes; Pessoa Junior,Adalberto; Magalhães,Pérola Oliveira. |
Proteases hydrolyze the peptide bonds of proteins into peptides and amino acids, being found in all living organisms, and are essential for cell growth and differentiation. Proteolytic enzymes have potential application in a wide number of industrial processes such as food, laundry detergent and pharmaceutical. Proteases from microbial sources have dominated applications in industrial sectors. Fungal proteases are used for hydrolyzing protein and other components of soy beans and wheat in soy sauce production. Proteases can be produced in large quantities in a short time by established methods of fermentation. The parameters such as variation in C/N ratio, presence of some sugars, besides several other physical factors are important in the development of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Proteases; Enzyme production; Fungal protease; Industrial application. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200337 |
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