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Transmission Electron Microscopy Study of the Neonatal Rat Tongue Mucosa Treated with Special Attention to the Bacteriae on the Epithelial Cell Membrane 77
Picoli,Lara Cristina; Lopes,Ruberval A; Semprini,Marisa; Sala,Miguel Angel; Ogawa,Koichi; Watanabe,Ii-sei.
The epithelial cell surfaces of neonatal Wistar rat tongue mucosa were examined by transmission electron microscopic method. The tissues were fixed by modified Karnovksy solution and embedded in Spurr resin. Thin sections of 90 nm thickness were cut and examined in the Jeol 1010 transmission electron microscopy. It was revealed that the queratinized squamous epithelial cells of the tongue mucosa exposed to cadmium presented groupings of numerous bacteriae which are attached to the flat cell membrane surfaces. The coccus and bacillus are attached to the cell membrane in organized rows or at random, as clearly seen in the TEM images. At high magnification, the transmission electron microscopic images showed that bacteriae are attached by a fibrillar...
Tipo: Journal article Palavras-chave: Epithelial cell; Tongue mucosa; Bacteria; Cadmium; Transmission electron microscopy.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000300006
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Morphometric study of the midgut epithelium in larvae of Diatraea saccharalis Fabricius (Lepidoptera: Pyralidae) 82
Pinheiro,Daniela O.; Silva,Reinaldo J.; Quagio-Grassiotto,Irani; Gregório,Elisa A..
The sugarcane borer, Diatraea saccharalis Fabricius, has great economical interest as it affects the culture and industrial use of the sugarcane. However, there are few studies concerning the internal morphology of this insect. This work aims to study morphometrically the midgut and the epithelium along their lenght, trying to characterize different regions. Midgut of last instar larvae was divided in three regions: anterior, middle and posterior, and the fragments were processed for light microscopic observation. Histological sections were analyzed in a computerized system concerning the length, width and area of the epithelium, their cells, and the midgut lumen. The obtained data were statistically analyzed by the Kruskal-Wallis test and by multivariate...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Morphometry; Epithelial cell; Insect; Sugarcane borer; Mesenteron.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2003000300012
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Placental maturation and expulsion in Holstein and Nelore cows 45
Martins,V.M.V.; Marques Junior,A.P.; Vasconcelos,A.C.; Martins,E.; Santos,R.L.; Lima,F.P.C..
Morphological changes in the placenta at periparturition and the involvement of apoptosis controlling cell populations in the placentome were evaluated in Holstein and Nelore cows. Twenty-two Holstein cows at the end of gestation with normal non-induced parturition (group I) and 10 Holstein cows with induced parturition (group II) were used in this study. In addition, groups III and IV were constituted by 10 Nelore cows at the end of gestation with normal non-induced parturition and 21 Nelore cows with induced parturition, respectivelly. Binucleate trophoblastic cells, epithelial caruncular cells and apoptotic bodies were quantified by light microscopy. Apoptosis was further evaluated by transmission electron microscopy, ELISA and DNA electrophoresis in...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Bovine; Placentome; Binucleate cell; Epithelial cell; Apoptosis.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352004000200004
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Spherites in the midgut epithelial cells of the sugarcane borer parasitized by Cotesia flavipes 47
de Oliveira Pinheiro,Daniela; Conte,Hélio; Aparecida Gregório,Elisa.
Diatraea saccharalis, the main pest of sugarcane, has been controlled by Cotesia flavipes. Very little is known about the effect of parasitism on the host organs, including the midgut. The Lepidoptera midgut epithelium is composed of columnar, goblet, regenerative, and endocrine cells. Spherites have been described in columnar and regenerative cells of several Lepidoptera species, and presented a lot of functional meaning. We identified spherites in the midgut epithelial cells of non-parasitized D. saccharalis larvae analyzed the effect of parasitism on spherite morphology and distribution along the length of the midgut. Midgut fragments of both non-parasitized and parasitized larvae were processed for transmission electron microscopy. All the midgut...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spherite; Epithelial cell; Ultrastructure; Lepidoptera; Parasitism.
Ano: 2008 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000100007
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