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| Registros recuperados: 31 | |
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| Bai,Xi; Yuan,Xianjun; Wen,Aiyou; Li,Junfeng; Bai,Yunfeng; Shao,Tao. |
| Background: Cold-active endo-1, 4-β-glucanase (EglC) can decrease energy costs and prevent product denaturation in biotechnological processes. However, the nature EglC from C. farmeri A1 showed very low activity (800 U/L). In an attempt to increase its expression level, C. farmeri EglC was expressed in Escherichia coli as an N-terminal fusion to protein S (ProS) from Myxococcus xanthus. Results: A novel expression vector, pET(ProS-EglC), was successfully constructed for the expression of C. farmeri EglC in E. coli. SDS-PAGE showed that the recombinant protein (ProS-EglC) was approximately 60 kDa. The activity of ProS-EglC was 12,400 U/L, which was considerably higher than that of the nature EglC (800 U/L). ProS-EglC was active at pH 6.5-pH 8.0,... |
| Tipo: Journal article |
Palavras-chave: Cellulose degradation; Cellulose; Cold-active enzyme; Endoglucanases; Enzymatic properties; Escherichia coli; Expression; Novel expression vector; N-terminal fusion; Protein S-tag; Recombinant protein. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600012 |
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| Díaz,Mauricio; Arenas,Gloria; Marshall,Sergio H. |
| Novel doublet molecules of cecropin A from Drosophila melanogaster were designed and constructed combining the regular (CECdir) with the inverted (CECret) coding sequence of the standard CEC A1 gene resulting in the following configurations: CECdir-CECret and CECret-CECdir. These two recombinant molecules were generated using a three-primer driven PCR reaction yielding composite single functional aminoacidic molecules with the coding sequences of CECdir linked in frame with the coding sequence of CECret and vice versa. In order to obtain these constructions, a retropeptide DNA-coding sequence was chemically synthesized to match the expected polarity of the newly generated CECret sequence. Both doublet antimicrobial peptides (drAMPs) were cloned in the T7... |
| Tipo: Journal article |
Palavras-chave: Antimicrobial peptides; Escherichia coli; Expression. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200006 |
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| Zhao,Da Qiu; Han,Chen Xia; Ge,Jin Tao; Tao,Jun. |
| Herbaceous peony (Paeonia lactiflora Pall.) is an excellent material for studying the formation of flower colour because of its abundant colour. The full-length cDNA of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene (UF5GT) containing 1629 bp nucleotides was obtained from P. lactiflora. The expression patterns of nine related anthocyanin biosynthetic genes (PlPSY, PlCHS, PlCHI, PlF3H, PlF3'H, PlDFR, PlANS, PlUF3GT and PlUF5GT) in diurnal variation petals showed that their expression peaks were basically at 15:00 and the expression patterns were consistent with the trend of sampling conditions except individual gene. And the highest expression levels were in PlCHS, PlDFR and PlUF3GT, which could be the candidates to regulate P. lactiflora flower... |
| Tipo: Journal article |
Palavras-chave: Anthocyanin; Expression; Glucosyltransferase; Herbaceous peony. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600009 |
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| Solís-Guzmán,María Gloria; Argüello-Astorga,Gerardo; López-Bucio,José; Ruiz-Herrera,León Francisco; López-Meza,Joel; Sánchez-Calderón,Lenin; Carreón-Abud,Yazmín; Martínez-Trujillo,Miguel. |
| Abstract Proteins of the Split ends (Spen) family are characterized by an N-terminal domain, with one or more RNA recognition motifs and a SPOC domain. In Arabidopsis thaliana, the Spen protein FPA is involved in the control of flowering time as a component of an autonomous pathway independent of photoperiod. The A. thaliana genome encodes another gene for a putative Spen protein at the locus At4g12640, herein named AtSpen2. Bioinformatics analysis of the AtSPEN2 SPOC domain revealed low sequence similarity with the FPA SPOC domain, which was markedly lower than that found in other Spen proteins from unrelated plant species. To provide experimental information about the function of AtSpen2, A. thaliana plants were transformed with gene constructs of its... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Spen; Arabidopsis; Vascular bundle; Expression. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400643 |
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| Rolland, Jean-luc; Medhioub, Walid; Vergnes, Agnes; Abi-khalil, Celina; Savar, Veronique; Abadie, Eric; Masseret, Estelle; Amzil, Zouher; Laabir, Mohamed. |
| To better understand the effect of Paralytic Shellfish Toxins (PSTs) accumulation in the digestive gland of the Pacific oyster, Crassostrea gigas, we experimentally exposed individual oysters for 48 h to a PSTs producer, the dinoflagellate Alexandrium catenella. In comparison to the effect of the non-toxic Alexandrium tamarense, on the eight apoptotic related genes tested, Bax and BI.1 were significantly upregulated in oysters exposed 48 h to A. catenella. Among the five detoxification related genes tested, the expression of cytochrome P450 (CYP1A) was shown to be correlated with toxin concentration in the digestive gland of oysters exposed to the toxic dinoflagellate. Beside this, we observed a significant increase in ROS production, a decrease in... |
| Tipo: Text |
Palavras-chave: Shellfish; Toxin; Biomarker; Expression; Phytoplankton. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00225/33606/32003.pdf |
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| Geffroy, Solene; Lechat, Marc-marie; Le Gac, Mickael; Rovillon, Georges-augustin; Marie, Dominique; Bigeard, Estelle; Malo, Florent; Amzil, Zouher; Guillou, Laure; Caruana, Amandine. |
| Paralytic shellfish poisoning (PSP) is a human foodborne syndrome caused by the consumption of shellfish that accumulate paralytic shellfish toxins (PSTs, saxitoxin group). In PST-producing dinoflagellates such as Alexandrium spp., toxin synthesis is encoded in the nuclear genome via a gene cluster (sxt). Toxin production is supposedly associated with the presence of a 4th domain in the sxtA gene (sxtA4), one of the core genes of the PST gene cluster. It is postulated that gene expression in dinoflagellates is partially constitutive, with both transcriptional and post-transcriptional processes potentially co-occurring. Therefore, gene structure and expression mode are two important features to explore in order to fully understand toxin production processes... |
| Tipo: Text |
Palavras-chave: Alexandrium; Saxitoxins; SxtA4; Copy number variation; SxtA; Expression; Isoform. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00682/79402/81944.pdf |
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| Mazurais, David; Coves, Denis; Papandroulakis, Nikos; Ortega, Aurelio; Desbruyeres, Elisabeth; Huelvan, Christine; Le Gall, Marie-madeleine; De La Gandara, Fernando; Cahu, Chantal. |
| The aim of this study was to determine whether mortality observed during the larval development of reared bluefin tuna (Thunnus thynnus) could be related to improper expression profiles of key genes involved in digestive or antioxidant response capabilities. Tuna larvae were sampled at hatching, 2, 5, 10, 15 and 20 dph (days post hatching) for the relative quantification of transcripts encoded by genes involved in digestive [trypsinogen 1 (TRYP1), alpha-amylase (AMY), aminopeptidase N (ANPEP)] and antioxidant [catalase (CAT)] functions. The levels of expression of ANPEP related to the development and maturation of intestinal function increased from 5 to 20 dph. Furthermore, AMY and TRYP1 genes, which are pancreatic enzymes implicated in carbohydrate and... |
| Tipo: Text |
Palavras-chave: Tuna; Gene; Expression; Development; Digestion; Antioxidant. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00171/28264/26521.pdf |
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| Bacca, Helene; Huvet, Arnaud; Fabioux, Caroline; Daniel, Jean-yves; Delaporte, Maryse; Pouvreau, Stephane; Van Wormhoudt, A; Moal, Jeanne. |
| To investigate the control at the mRNA level of glycogen metabolism in the cupped oyster Crassostrea gigas, we report in the present paper the cloning and characterization of glycogen phosphorylase and synthase cDNAs (Cg-GPH and Cg-GYS, respectively, transcripts of main enzymes for glycogen use and storage), and their first expression profiles depending on oyster tissues and seasons. A strong expression of both genes was observed in the labial palps and the gonad in accordance with specific cells located in both tissues and ability to store glucose. Cg-GPH expression was also found mainly in muscle suggesting ability to use glycogen as readily available glucose to. supply its activity. For seasonal examinations, expression of Cg-GYS and Cg-GPH genes... |
| Tipo: Text |
Palavras-chave: Reproduction; Regulation; Oyster; Glycogen; Expression; Gene; Energy; Crassostrea gigas; Bivalve. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-435.pdf |
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| Kumagai, A.; Zhang, G.; Jia, H.; Nakamura, C.; Zhang, H.; Goo, Y-K.; Aboge, G. A.; Terkawi, M. A.; Zhou, J.; Nishikawa, Y.; Xuan, X.. |
| A gene encoding Babesia gibsoni thrombospondin-related adhesive protein (BgTRAP), known as a vaccine candidate, was stably expressed in Toxoplasma gondii (Tg/BgTRAP). The molecular weight and the antigenic reaction of recombinant BgTRAP expressed by the Tg/BgTRAP were similar to the original ones expressed by B. gibsoni. To evaluate the antigenicity of the recombinant BgTRAP expressed by T. gondii, the lysates of the recombinant parasite tachyzoites were intraperitoneally injected into mice. The serum collected from Tg/BgTRAP-immunized mouse could react to B. gibsoni parasites, while the serum collected from wild-type T. gondii tachyzoites (Tg/wt)-immunized mice did not. These results indicate that T. gondii could provide a new tool to produce foreign... |
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Palavras-chave: Toxoplasma gondii; Babesia gibsoni; BgTRAP; Expression; Vaccine. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4496 |
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| Feng¹,RJ; Lu,LF; Yuan,KH; Cheng,P; Zhang,LL; Qi,JF; Ren,Y; Xu,XL; Zhang,XB; Zhou,LY; Zhang,YD. |
| A banana (Musa AAA, Cavendish subgroup cv. Brazil) cDNA encoding a putative rubredoxin-like protein (MaRd1) was obtained from total RNA isolated from cold-treated banana leaves using rapid amplification of cDNA ends (RACE) technique. MaRd1 cDNA contained 597 nucleotides encoding 198 amino acids in the open reading frame. MaRd1 protein showed 56% amino acid identity with that of Pyrococcus furiosus rubredoxin (P24297). A chloroplast transit peptide and a transmembrane region were detected at the N-terminus and the C-terminus, respectively, of the deduced amino acid sequence of MaRd1 gene. Southern blotting revealed the occurrence of at least two copies of MaRd1 in the banana genome. Real time quantitative RT-PCR analysis revealed that the expression of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rubredoxin; Expression; Banana; Stress response. |
| Ano: 2010 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572010000200008 |
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| Wang,Y; Li,YB; Yang,HM; Wang,ZY. |
| ABSTRACT We investigated the egg production, changes in luteinizing hormone (LH), follicle-stimulating hormone (FSH), gonadal hormones, and their mRNA levels in the hypothalamic-pituitary-gonadal axis of White King pigeons submitted to different photoperiods. The treatments consisted of three photoperiods (8 h light (L):16 h dark (D), 12L:12D, and 16L:8D), with three replicates of twelve pairs of adult pigeons. The birds were exposed the photoperiods for 45 days. Egg production performance was recorded daily. Six pigeon pairs per replicate were selected for plasma collection, and six pigeon pairs per replicate for the resection of the hypothalamic-pituitary-gonadal (HPG) axis. Egg production was significantly improved by long-day lighting (16L:8D), while... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Day lighting; Expression; Hormone; Pigeon; Reproduction. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000400314 |
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| Fan,Caiyun; Zhang,Yunhai; Wang,Juhua; Cheng,Jianbo. |
| ABSTRACT This study examined the effects of purmorphamine and cyclopamine, classical agonists and inhibitors of the Hedgehog (Hh) signaling pathway, in the adipogenic differentiation of porcine adipose-derived mesenchymal stem cells (AMSC) to investigate the roles underlying adipogenic differentiation in AMSC. Porcine-derived AMSC were established, and the Hh signaling pathway was activated or inhibited by treatment with purmorphamine or cyclopamine. The adipogenic differentiation of the porcine AMSC was then analyzed by Oil Red O staining. The expression levels of Hh signaling pathway factors and adipogenic transcription factors were determined using quantitative real-time polymerase chain reaction and western blot analysis. We verified that the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Adipogenesis; Differentiation; Expression; Pig. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100415 |
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| MEDINA,RODOLFO A.; OWEN,GARETH I.. |
| Mammalian cells depend on glucose as a major substrate for energy production. Glucose is transported into the cell via facilitative glucose transporters (GLUT) present in all cell types. Many GLUT isoforms have been described and their expression is cell-specific and subject to hormonal and environmental control. The kinetic properties and substrate specificities of the different isoforms are specifically suited to the energy requirements of the particular cell types. Due to the ubiquitousness of these transporters, their differential expression is involved in various disease states such as diabetes, ischemia and cancer. The majority of cancers and isolated cancer cell lines over-express the GLUT family members which are present in the respective tissue of... |
| Tipo: Journal article |
Palavras-chave: GLUT; Glucose transporters; Expression; Cancer; Estrogen; Progesterone; Cell lines. |
| Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000100004 |
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| Guo,Lina. |
| ABSTRACT Olfactory receptors are essential for recognition and detection of odor in honeybees. Although we have cloned and characterized the sequence of olfactory receptor AcerOr1 before, the tissue distribution and location of this gene in the nurse and the forager worker Apis cerana cerana were not very clear. To further investigate this information of AcerOr1 gene, we analyzed its expression and localization. The results showed that AcerOr1 mRNA was predominantly expressed in the antennae of nurse and forager bees, while the AcerOr1 protein was predominant in thorax, and its expression in antennae was higher in forager than in nurse. IHC revealed that AcerOr1 mainly localized in the olfactory neurons of the antennae. In addition, the staining intensity... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apis cerana cerana; Olfactory receptor; AcerOr1; Expression; Localization; In vivo. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100440 |
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| Ling,Zheng; Qi-Qing,Jiao; Yu,Wang; Fei,Bian; Shu-Jie,Qu; Shu-Bo,Wan; Zhen-Ying,Peng; Yu-Ping,Bi. |
| The increased incidence of diabetes, coupled with the introduction of alternative insulin delivery methods that rely on higher doses, is expected to result in a substantial escalation in the future demand for affordable insulin. Plant-based systems offer a safe and economical method for producing pharmaceutical proteins. We used peanut (Arachis hypogaea L.) as bio-reactors to express biosafe, stable proinsulin. We designed two proinsulin analogues (FAIA and LAIA) with substitutions in their amino acid sequences. The fast-acting insulin analogue (FAIA) contains a Gly inserted between Cys19 and Gly20, as well as a Pro28Asp substitution, in the B chain. The long-acting insulin analogue (LAIA) contains a Gly inserted between Cys19 and Gly20 and two Arg... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Proinsulin; Peanut; Diabetes; Expression. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100323 |
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| Cui,Y.; Zhou,P.; Peng,J.; Peng,M.; Zhou,Y.; Lin,Y.; Liu,L.. |
| Our objective was to clone, express and characterize adult Dermatophagoides farinae group 1 (Der f 1) allergens to further produce recombinant allergens for future clinical applications in order to eliminate side reactions from crude extracts of mites. Based on GenBank data, we designed primers and amplified the cDNA fragment coding for Der f 1 by nested-PCR. After purification and recovery, the cDNA fragment was cloned into the pMD19-T vector. The fragment was then sequenced, subcloned into the plasmid pET28a(+), expressed in Escherichia coli BL21 and identified by Western blotting. The cDNA coding for Der f 1 was cloned, sequenced and expressed successfully. Sequence analysis showed the presence of an open reading frame containing 966 bp that encodes a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dermatophagoides farinae; Der f 1; Recombinant allergens; Cloning; Expression; Bioinformatics. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500006 |
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| Registros recuperados: 31 | |
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