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| Candeias,Marcela Leite; Alvarenga,Marco Antonio; Carmo,Márcio Teoro do; Ferreira,Heder Nunes; Maior,Mônica Russo Souto; Torres Filho,Rodolpho de Almeida; Rodrigues,André Luís Rios; Brandão,Felipe Zandonadi. |
| The effect of the utilization of three semen protocols (Inra 82®, Merck Gema and Botu-crio®) and two filling techniques (0.25 and 0.50 mL straws) in Mangalarga Marchador stallions were studied in this experiment. Sperm parameters were assessed during processing and post-freezing. No interactions between the protocols and type of filling were observed, so they were assessed separately. Sperm parameters were not altered when the extender was added to the centrifugation; however, there was reduction of motility and strength when freezing extenders were added. The Botu-crio® protocol preserved the parameters of total and progressive sperm motility, smoothed path velocity (µm/s), straight line velocity (µm/s), track velocity (µm/s) and the average and fast... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ejaculated; Equine; Extender; Straw. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000900004 |
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| Ferreira,Valéria da Silva; Mello,Marco Roberto Bourg de; Fonseca,Carlos Elysio Moreira da; Dias,Állan César Ferreira; Cardoso,Jéssica Machado; Silva,Rebecca Barbosa; Martins Júnior,Wagner Pereira. |
| The objective of this study was to evaluate the effects of egg yolk and seminal plasma on the viability of cryopreserved goat semen. To this end, four fertile Saanen bucks, aged between 10 months and 1 year, and weighing 18 to 25 kg, were used. Semen was collected from each buck by the artificial vagina method at the end of breeding season (June-July). The extender used was the yolk citrate, which was split into two equal aliquots: 5% egg yolk (2.5 mL egg yolk: 47.5 mL citrate solution) were added to one of the samples and 10% egg yolk (5.0 mL egg yolk: 45.0 mL citrate solution) were added to another. The sperm motility and vigor after thawing and post thermal resistance test (TRT) were evaluated and the data were subjected to analysis of variance and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Buck; Centrifugation; Extender. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014001000513 |
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| SANCHES,EDUARDO G.; OLIVEIRA,IDILI R.; SERRALHEIRO,PEDRO C. DA SILVA; CERQUEIRA,VINICIUS R.. |
| This study aimed to develop a protocol of semen cryopreservation of the mutton snapper Lutjanus analis. The interaction between three extenders ( pH 6.1; 7.8 and 8.2) , two concentrations of dimethyl sulfoxide ( DMSO, 5 and 10%) and three cooling rates ( -90; -60 and -30°C.min−1) on the sperm motility rate and motility time were analyzed by a factorial experiment. A sample of 30 fishes ( 1,261 ± 449 g) collected in the nature was kept in floating net cages. The semen was frozen by using cryogenic straws, in nitrogen vapour and transferred, later, to liquid nitrogen. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The highest sperm motility rate and motility time ( P < 0.05) was achieved by combining extender C (... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryoprotector; Extender; Lutjanus analis; Reproduction; Sperm. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000301083 |
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| Silva,A.R.; Cardoso,R.C.S.; Silva,L.D.M.. |
| Standardized sperm concentration and volume:volume extension were compared as dilution rates for canine semen freezing. Six proven stud dogs were submitted to two seminal collections by manual stimulation. Semen was evaluated and extended in tris plus egg-yolk and glycerol according to two different dilution rates. The first one was based on a standardized sperm concentration of 200x10(6) spermatozoa/ml and the second was a volume:volume extension at a proportion of one part semen to one part extender. Semen was frozen, stored in liquid nitrogen and thawed after one week. Sperm motility and vigor were appraised after each stage of the process and at 15 and 30min post-thawing. Sperm morphology was analyzed after collection and thawing. No differences were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Semen; Extender; Freezing; Dilution. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600010 |
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| Melo,M.I.V.; Henry,M.; Beker,A.R.C.L.. |
| The hypoosmotic test (HO) was used to evaluate plasmatic membrane integrity of equine chilled semen and to estimate the correlation between the results of the HO test and those from applied routine exams of semen. The semen of seven stallions was preserved at 5ºC in three different extenders. Each ejaculate was diluted in three extenders, Kenney (K), Baken with 3% of egg yolk (B3) and Baken with 10% of egg yolk (B10), and chilled at 5ºC. The total motility (MT), progressive motility (MP), spermatic vigor (V), normal spermatic morphology (NOR) and HO test were used to evaluate semen immediately after collection and at 24 hour-intervals. Spermatic longevity was defined as the time taken to the progressive motility to decline under 10%. Semen diluted in B3... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Equine; Hypoosmotic test; Chilled semen; Extender. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600009 |
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| Miraglia,Fabiana; Morais,Zenaide Maria; Cortez,Adriana; Melville,Priscilla Anne; Marvullo,Maria Fernanda Vianna; Richtzenhain,Leonardo José; Visintin,José Antonio; Vasconcellos,Silvio Arruda. |
| Inactivation of leptospires in pools of semen from three Holstein Friesian bulls, collected in an artificial vagina, was investigated. Spermatic concentration was adjusted in egg yolk citrate extender, submitted to the following treatments: A (control; without antibiotics); B (penicillin, 1,000 UI/mL - streptomycin, 1,000 µg/mL); C (amoxicillin, 1,000 µg/mL); D (ceptiofur sodium, 1,000 µg/mL); E (amoxicillin 1,000 µg/mL - ceptiofur sodium 1,000 µg/mL). Leptospires (2.0 x 10(6) leptospires/mL) were added into the diluted semen. Recovery of leptospires was obtained in modified EMJH semi-solid medium with and without antibiotics. The antibiotics in the concentrations used did not affect means of percentage of progressive motility and individual progressive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospires; Semen; Antibiotics; Extender. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200011 |
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