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	Provedor de dados BABT (1) BJM (1) Autor Banerjee,Rintu (1) Dutta,Jayati Ray (1) Martins,Maurilio L. (1) Pinto,Uelinton M. (1) Riedel,Katharina (1) Vanetti,Maria C.D. (1) Mais... Palavra-chave Extracellular protease (2) Extracellular lipase (1) Food deterioration (1) Kinetic characterization (1) Physicochemical (1) Pseudomonas fluorescens (1) Pseudomonas sp. (1) Purification (1) Raw milk (1) Ultraviolet mutagenesis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2015 (1) 2006 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Isolation and characterization of a newly isolated Pseudomonas mutant for protease production BABT Dutta,Jayati Ray; Banerjee,Rintu. A potent bacterium for extracellular protease production was isolated from local soil and identified as Pseudomonas sp. RAJR 044. A mutant of this strain JNGR 242 with protease productivity 2.5 fold higher was obtained by ultraviolet irradiation under experimentally optimized conditions of pH 7.0, temperature of 34ºC, inoculum volume of 1.0 mL and incubation time of 24 hours. Comparative analysis of the chemical characteristics i.e. assimilation of carbon and nitrogen sources were also carried out. Maximum growth of the mutant strain in 2% gelatin agar plate was obtained in presence of dextrose (2%), maltose (2%), ammonium sulfate (2%) and potassium nitrate (2%) whereas, that of the parent strain was found in sucrose (2%) and ammonium nitrate (2%). The... Tipo: Info:eu-repo/semantics/article Palavras-chave: Extracellular protease; Purification; Kinetic characterization; Ultraviolet mutagenesis; Pseudomonas sp.; Physicochemical. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100005 Milk-deteriorating exoenzymes from Pseudomonas fluorescens 041 isolated from refrigerated raw milk BJM Martins,Maurilio L.; Pinto,Uelinton M.; Riedel,Katharina; Vanetti,Maria C.D.. The practice of refrigerating raw milk at the farm has provided a selective advantage for psychrotrophic bacteria that produce heat-stable proteases and lipases causing severe quality problems to the dairy industry. In this work, a protease (AprX) and a lipase (LipM) produced by Pseudomonas fluorescens 041, a highly proteolytic and lipolytic strain isolated from raw milk obtained from a Brazilian farm, have been purified and characterized. Both enzymes were purified as recombinant proteins from Escherichia coli. The AprX metalloprotease exhibited activity in a broad temperature range, including refrigeration, with a maximum activity at 37 °C. It was active in a pH range of 4.0 to 9.0. This protease had maximum activity with the substrates casein and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Raw milk; Food deterioration; Pseudomonas fluorescens; Extracellular protease; Extracellular lipase. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000100207 Registros recuperados: 2 Primeira ... 1 ... Última

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