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| Mozafari,Roghayeh; Kyrylenko,Sergiy; Castro,Mateus Vidigal; Ferreira Jr,Rui Seabra; Barraviera,Benedito; Oliveira,Alexandre Leite Rodrigues. |
| Abstract Background Peripheral nerve injury is a worldwide clinical problem, and the preferred surgical method for treating it is the end-to-end neurorrhaphy. When it is not possible due to a large nerve gap, autologous nerve grafting is used. However, these surgical techniques result in nerve regeneration at highly variable degrees. It is thus very important to seek complementary techniques to improve motor and sensory recovery. One promising approach could be cell therapy. Transplantation therapy with human embryonic stem cells (hESCs) is appealing because these cells are pluripotent and can differentiate into specialized cell types and have self-renewal ability. Therefore, the main objective of this study was to find conditions under which functional... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neurorrhaphy; Sciatic nerve; Human embryonic stem cells; Fibrin sealant; FGF2. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100305 |
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| Forti,Fábio L.; Costa,Érico T.; Rocha,Kátia M.; Moraes,Miriam S.; Armelin,Hugo A.. |
| The mouse Y1 adrenocortical tumor cell line is highly responsive to FGF2-(Fibroblast Growth Factor 2) and possesses amplified and over-expressed c-Ki-ras proto-oncogene. We previously reported that this genetic lesion leads to high constitutive levels of activation of the c-Ki-Ras-GTP->PI3K->Akt signaling pathway (Forti et al. 2002). On the other hand, activation levels of another important pathway downstream of c-Ki-Ras-GTP, namely, Raf->MEK->ERK, remain strictly dependent on FGF2 stimulation (Rocha et al. 2003). Here we show that, first, FGF2 transiently up-regulates the c-Ki-Ras-GTP->PI3K->Akt pathway, in spite of its high basal levels. Second, c-Ki-Ras-GTP transient up-regulation likely underlies activation of the ERK1/2 pathway by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: FGF2; Adrenocortical tumor cells; C-ki-ras; C-h-ras; ERK. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000200005 |
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| Lotfi,C.F.P.; Lepique,A.P.; Forti,F.L.; Schwindt,T.T.; Eichler,C.B.; Santos,M.O.; Rebustini,I.T.; Hajj,G.N.M.; Juliano,L.; Armelin,H.A.. |
| This article reviews recent results of studies aiming to elucidate modes of integrating signals initiated in ACTH receptors and FGF2 receptors, within the network system of signal transduction found in Y1 adrenocortical cells. These modes of signal integration should be central to the mechanisms underlying the regulation of the G0->G1->S transition in the adrenal cell cycle. FGF2 elicits a strong mitogenic response in G0/G1-arrested Y1 adrenocortical cells, that includes a) rapid and transient activation of extracellular signal-regulated kinases-mitogen-activated protein kinases (ERK-MAPK) (2 to 10 min), b) transcription activation of c-fos, c-jun and c-myc genes (10 to 30 min), c) induction of c-Fos and c-Myc proteins by 1 h and cyclin D1 protein by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ACTH; FGF2; Signal transduction; MAP kinases; Early response genes. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001000002 |
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| Qian,W.F.; Guan,W.X.; Gao,Y.; Tan,J.F.; Qiao,Z.M.; Huang,H.; Xia,C.L.. |
| In order to investigate signal transduction and activation of transcription 3 (STAT3) signaling on angiogenesis in colorectal carcinoma (CRC) after inhibiting STAT3 expression, we constructed the HT-29-shSTAT3 cell line by lentivirus-mediated RNAi. Cell growth was assessed with MTT and the cell cycle distribution by flow cytometry. CRC nude mouse models were established and tumor growth was monitored periodically. On day 30, all mice were killed and tumor tissues were removed. Microvessel density (MVD) was determined according to CD34-positive staining. The expression of vascular endothelial growth factor A (VEGFA), matrix metalloproteinase-2 (MMP2) and basic fibroblast growth factor (FGF2) was monitored by quantitative real-time PCR and Western blot... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: STAT3; RNA interference; Colorectal carcinoma; Angiogenesis; VEGFA; MMP2; FGF2. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001200005 |
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