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New ammonia lyases and amine transaminases: Standardization of production process and preparation of immobilized biocatalysts Electron. J. Biotechnol.
Casablancas,Antoni; Cárdenas-Fernández,Max; Álvaro,Gregorio; Benaiges,Maria Dolors; Caminal,Glòria; Mas,Carles de; González,Glòria; López,Carmen; López-Santín,Josep.
Background: New enzymes for biotransformations can be obtained by different approaches including directed mutagenesis and in vitro evolution. These mutants have to be efficiently produced for laboratory research on bioreactions as well as for process development. In the framework of a European ERA-IB project, two different types of enzymes (ammonia lyases and aminotransferases) have been selected as biocatalysts for the synthesis of industrially relevant amines. New mutant enzymes have been obtained: a) aspartases able to recognize β-amino acids; b) ω-transaminases with improved activity. The objectives are to find out a common operational strategy applicable to different mutants expressed in E. coli with the same initial genetic...
Tipo: Journal article Palavras-chave: Amine transaminases; Ammonia lyases; E. coli; Fed-batch; Immobilized biocatalysts.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300004
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BIOHYDROGEN PRODUCTION IN AnSBBR IN FED-BATCH FROM STARCH EFFLUENT: INFLUENCE OF ORGANIC LOAD REA
Tonello,Tamiris U.; Andreani,Cristiane L.; Mari,Angelo G.; Fernandes,José R.; Gomes,Simone D..
ABSTRACT The hydrogen is a fuel with a high energy density. Studies have sought to verify the potential of different residues and configurations of reactors in the production of biohydrogen. This study evaluated the influence of the organic loading rate on the production of biohydrogen from cassava starch wastewater (CSW) in anaerobic sequencing batch biofilm reactors (AnSBBR) operated in a fed-batch. In the tests, the organic loading rates of 9.0; 13.5 and 18.0 gCT L−1 d−1 were used, at cycle times (CT) of 4; 4 and 2 h (tests I, II and III, respectively). The reactor was inoculated with sludge from a pilot anaerobic reactor used in the treatment of CSW, heat treated (heated at 90°C for 15 min). With the increase of the organic load (condition I to II)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fed-batch; Fermentative process; Bioenergy; Cassava starch wastewater.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-69162018000500768
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Xylitol production by Candida parapsilosis under fed-batch culture BABT
Furlan,Sandra A.; Castro,Heizir F. de.
Xylitol production by Candida parapsilosis was investigated under fed-batch cultivation, using single (xylose) or mixed (xylose and glucose) sugars as substrates. The presence of glucose in the medium induced the production of ethanol as secondary metabolite and improved specific rates of growth, xylitol formation and substrate consumption. Fractionated supply of the feed medium at constant sugar concentration did not promote any increase on the productivity compared to the single batch cultivation.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida parapsilosis; Glucose; Xylose; Xylitol; Fed-batch.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000200003
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Yeast biomass production: a new approach in glucose-limited feeding strategy BJM
Vieira,Érika Durão; Andrietta,Maria da Graça Stupiello; Andrietta,Silvio Roberto.
The aim of this work was to implement experimentally a simple glucose-limited feeding strategy for yeast biomass production in a bubble column reactor based on a spreadsheet simulator suitable for industrial application. In biomass production process using Saccharomyces cerevisiae strains, one of the constraints is the strong tendency of these species to metabolize sugars anaerobically due to catabolite repression, leading to low values of biomass yield on substrate. The usual strategy to control this metabolic tendency is the use of a fed-batch process in which where the sugar source is fed incrementally and total sugar concentration in broth is maintained below a determined value. The simulator presented in this work was developed to control molasses...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast; Biomass; Fed-batch; Process simulation; Saccharomyces cerevisiae.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000200035
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