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	Provedor de dados BJMBR (1) Electron. J. Biotechnol. (1) Autor Avó,L.R.S. (1) Bueno,P.G. (1) Leal,A.M.O. (1) Li,Liang (1) Li,Xinxin (1) Liu,Hehe (1) Nonaka,K.O. (1) Selistre-Araújo,H.S. (1) Silva,R.N. (1) Sun,Lingli (1) Wang,Haohan (1) Wang,Jiwen (1) Yang,Chao (1) Zheng,Yi (1) Mais... Palavra-chave Follistatin (2) Activin A (1) Exercise (1) Muscle hypertrophy (1) Nonalcoholic fatty liver disease (1) Obesity (1) Overexpression (1) Steatosis (1) Transfection efficiency (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2014 (2) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Construction of a eukaryotic expression vector for pEGFP-FST and its biological activity in duck myoblasts Electron. J. Biotechnol. Li,Xinxin; Wang,Jiwen; Liu,Hehe; Wang,Haohan; Sun,Lingli; Yang,Chao; Li,Liang; Zheng,Yi. Background Follistatin (FST), a secreted glycoprotein, is intrinsically linked to muscle hypertrophy. To explore the function of duck FST in myoblast proliferation and differentiation, the pEGFP-FST eukaryotic expression vector was constructed and identified. The biological activities of this vector were analyzed by transfecting pEGFP-FST into cultured duck myoblasts using Lipofectamine™ 2000 and subsequently determining the mRNA expression profiles of FST and myostatin (MSTN). Results The duck pEGFP-FST vector was successfully constructed and was confirmed to have high liposome-mediated transfection efficiency in duck myoblasts. Additionally, myoblasts transfected with pEGFP-FST had a higher biological activity. Significantly, the overexpression of FST in... Tipo: Journal article Palavras-chave: Follistatin; Muscle hypertrophy; Overexpression; Transfection efficiency. Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000500006 Effect of physical training on liver expression of activin A and follistatin in a nonalcoholic fatty liver disease model in rats BJMBR Silva,R.N.; Bueno,P.G.; Avó,L.R.S.; Nonaka,K.O.; Selistre-Araújo,H.S.; Leal,A.M.O.. Nonalcoholic fatty liver disease (NAFLD) is characterized by fat accumulation in the liver and is associated with obesity and insulin resistance. Activin A is a member of the transforming growth factor beta (TGF)-β superfamily and inhibits hepatocyte growth. Follistatin antagonizes the biological actions of activin. Exercise is an important therapeutic strategy to reduce the metabolic effects of obesity. We evaluated the pattern of activin A and follistatin liver expression in obese rats subjected to swimming exercise. Control rats (C) and high-fat (HF) diet-fed rats were randomly assigned to a swimming training group (C-Swim and HF-Swim) or a sedentary group (C-Sed and HF-Sed). Activin βA subunit mRNA expression was significantly higher in HF-Swim than in... Tipo: Info:eu-repo/semantics/article Palavras-chave: Activin A; Follistatin; Steatosis; Nonalcoholic fatty liver disease; Obesity; Exercise. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900746 Registros recuperados: 2 Primeira ... 1 ... Última

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