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| Paredes,Noelia I.; Consolo,Verónica F.; Pancotto,Verónica A.; Fritz,Christian; Barrera,Marcelo D.; Arambarri,Angélica M.; Salerno,Graciela L.. |
| Southern Hemisphere peatlands store substantial amounts of soil carbon. Despite their importance in the global carbon cycle, little is known about decomposition processes and the associated fungal diversity. The present study describes the composition of fungal assemblage in two depths from a cushion peatland of predominating Astelia (Asteliaceae) and Donatia (Donatiaceae) species in Moat, Tierra del Fuego. From 48 samples processed, we obtained 338 isolates. Using different culturing methodologies, through direct and microscopic observation and using molecular methods we identified 38 fungal species and 18 genera of Ascomycetes and Zygomycetes. Isolates belonging to Ascomycetes were the most abundant, with dominance of Penicillium and Trichoderma. We... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Astelia-Donatia; Diversity; Fungal community; PCR- RFLP; Peatland. |
| Ano: 2014 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0011-67932014000100007 |
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| Cheng,Fei; Hou,Lin; Woeste,Keith; Shang,Zhengchun; Peng,Xiaobang; Zhao,Peng; Zhang,Shuoxin. |
| Abstract Humic substances in soil DNA samples can influence the assessment of microbial diversity and community composition. Using multiple steps during or after cell lysis adds expenses, is time-consuming, and causes DNA loss. A pretreatment of soil samples and a single step DNA extraction may improve experimental results. In order to optimize a protocol for obtaining high purity DNA from soil microbiota, five prewashing agents were compared in terms of their efficiency and effectiveness in removing soil contaminants. Residual contaminants were precipitated by adding 0.6 mL of 0.5 M CaCl2. Four cell lysis methods were applied to test their compatibility with the pretreatment (prewashing + Ca2+ flocculation) and to ultimately identify the optimal cell... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cell lysis; DNA extraction method; DNA purity; Terminal restriction fragment length polymorphism; Fungal community. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400817 |
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