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	Provedor de dados Scientia Agricola (1) Electron. J. Biotechnol. (1) Autor Hefferon,Kathleen L (1) Moon,Yong-Sun (1) Quecini,Vera Maria (1) Vieira,Maria Lúcia Carneiro (1) Palavra-chave GUS expression (2) Agrobacterium-mediated expression (1) BeYDV-based vector (1) Direct DNA transfer (1) Forage legume (1) Gene amplification (1) Plant transformation (1) Rep expression (1) Transgenic Arabidopsis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2007 (1) 2001 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Amplification of genome-integrated BeYDV replicons by transient expression of Rep in Arabidopsis thaliana Electron. J. Biotechnol. Moon,Yong-Sun; Hefferon,Kathleen L. Bean yellow dwarf geminivirus (BeYDV) has been used as a potential vector to improve foreign gene expression, specifically, to achieve higher yields of vaccine proteins in plants. Previously, we have shown that when the BeYDV replication initiator protein Rep was provided in trans, replication and gene expression of GUS were enhanced enormously from a BeYDV expression vector in a transient assay system. In this paper, transgenic lines of Arabidopsis (cv. Columbia) were generated harboring the BeYDV cis-acting elements required for replication. Constructs encoding BeYDV Rep or intronless Rep open reading frames (ORFs) were transiently introduced into transgenic plants via Agrobacterium-mediated infiltration in order to examine the relative levels of... Tipo: Journal article Palavras-chave: Agrobacterium-mediated expression; BeYDV-based vector; Gene amplification; GUS expression; Rep expression; Transgenic Arabidopsis. Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400001 Transient gene expression in electroporated intact tissues of Stylosanthes guianensis (Aubl.) Sw. Scientia Agricola Quecini,Vera Maria; Vieira,Maria Lúcia Carneiro. Genetic transformation though protoplast electroporation has been established for commercially important plant species. In this work, explant sources, electric field strengths, electroporation buffers, DNA forms and osmotic pretreatment were assayed in order to optimize transient reporter gene expression in electroporated tissues of Stylosanthes guianensis, a tropical forage legume. Higher transformation rates were obtained employing cotyledonary explants and an electric field strength of 250 V cm-1. Linear plasmid DNA, chloridefree electroporation buffer and osmotic pretreatment with 1.6 mol L-1 mannitol also improved transient transformation but non-significantly. Transgene specific PCR amplification was employed to prove the transformed status of the... Tipo: Info:eu-repo/semantics/article Palavras-chave: GUS expression; Direct DNA transfer; Plant transformation; Forage legume. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162001000400018 Registros recuperados: 2 Primeira ... 1 ... Última

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