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| Registros recuperados: 42 | |
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| Alcántara Mendoza, Susana. |
| Una nueva enfermedad del maíz apareció en el estado de Veracruz, México durante 2003-2004. Síntomas de amarillamiento, enrojecimiento y posterior quemado de hojas, proliferación de mazorcas, jilotes raquíticos, falta de fecundación y granos vanos fueron parte del síndrome de la enfermedad. El fitoplasma Maize Bushy Stunt (MBS) se identifico por PCR se detectó en plantas con los síntomas antes descritos, en los municipios de Tlalixcoyan, Cosoleacaque y Paso de Ovejas en 2006 y 2007. Los genotipos CP-562, 30F83, 30F92, Orca, 30F96, Nutria y Asgrow 7573 resultaron positivos a la presencia del MBS, mientras que en 3086, 30F97 y CP-560 no se detectó la presencia del MBS en una plantación experimental en plantas sintomáticas en Tlalixcoyan, Ver. en 2006 y 2007.... |
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Palavras-chave: Maize Bushy Stunt; PCR; Incidencia; Resistencia genética; Genoma; Homología; Incidence; Genetic resistance; Genome; Homology; Maestría en Ciencias; Postgrado en Fitosanidad; Fitopatología. |
| Ano: 2009 |
URL: http://hdl.handle.net/10521/53 |
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| Alcántara Mendoza, Susana. |
| Una nueva enfermedad del maíz apareció en el estado de Veracruz, México durante 2003-2004. Síntomas de amarillamiento, enrojecimiento y posterior quemado de hojas, proliferación de mazorcas, jilotes raquíticos, falta de fecundación y granos vanos fueron parte del síndrome de la enfermedad. El fitoplasma Maize Bushy Stunt (MBS) se identifico por PCR se detectó en plantas con los síntomas antes descritos, en los municipios de Tlalixcoyan, Cosoleacaque y Paso de Ovejas en 2006 y 2007. Los genotipos CP-562, 30F83, 30F92, Orca, 30F96, Nutria y Asgrow 7573 resultaron positivos a la presencia del MBS, mientras que en 3086, 30F97 y CP-560 no se detectó la presencia del MBS en una plantación experimental en plantas sintomáticas en Tlalixcoyan, Ver. en 2006 y 2007.... |
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Palavras-chave: Maize Bushy Stunt; PCR; Incidencia; Resistencia genética; Genoma; Homología; Incidence; Genetic resistance; Genome; Homology; Maestría; Postgrado en Fitosanidad; Fitopatología. |
| Ano: 2009 |
URL: http://hdl.handle.net/10521/53 |
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| Li,Biaoru. |
| Recent advances in functional genomics allow us to estimate the expression of several thousands of genes in the mammalian genome. Techniques such as microarrays, expressed tag sequencing (EST), serial analysis of gene expression (SAGE), subtractive cloning and differential display (DD), and two-dimensional electrophoresis gel have been extensively used to screen and analyze parallel gene expression. Some pathological processes, for example, tumorigenesis and solid tumour growth, in which the former is derived from a single-cell and the latter has a mixed-cell problem, present new challenges to the limit of these functional genomic techniques. To fully understand the functions of cells in tumorigenesis or in heterogeneous solid tumour masses, it is... |
| Tipo: Journal article |
Palavras-chave: Genome; Genomic expression; Genomic expression analysis at single cell; Single cell. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100011 |
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| Rodríguez Yunta,Eduardo; Valdebenito Herrera,Carolina; Misseroni,Adelio; Fernández Milla,Lautaro; Outomuro,Delia; Schiattino Lemus,Irene; Ferrer Lues,Marcela; Lolas Stepke,Fernando. |
| The present reflection refers to data obtained about the social representations of genomic research and its applications through interviews with legislators and lawyers, biomedical researchers and civilians and the review of scientific and legal literature in four Latin American countries: Argentine, Chile, Mexico and Peru. Several issues are addressed: little access to prevention and therapeutic methods, lack of equity in health benefits, commercialization of gene sequences through patents which leads to commercial exploitation of underdeveloped countries, the possibility of physical or psychological damage or genetic discrimination, the possibility of genetic modifications or abortion for eugenic reasons, the necessity of safeguarding confidentiality,... |
| Tipo: Journal article |
Palavras-chave: Cloning; Genome; Social representations; Transgenic. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000300003 |
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| Vianna,Juliana A.; Noll,Daly; Mura-Jornet,Isidora; Valenzuela-Guerra,Paulina; González-Acuña,Daniel; Navarro,Cristell; Loyola,David E.; Dantas,Gisele P. M.. |
| Abstract Microsatellites are valuable molecular markers for evolutionary and ecological studies. Next generation sequencing is responsible for the increasing number of microsatellites for non-model species. Penguins of the Pygoscelis genus are comprised of three species: Adélie (P. adeliae), Chinstrap (P. antarcticus) and Gentoo penguin (P. papua), all distributed around Antarctica and the sub-Antarctic. The species have been affected differently by climate change, and the use of microsatellite markers will be crucial to monitor population dynamics. We characterized a large set of genome-wide microsatellites and evaluated polymorphisms in all three species. SOLiD reads were generated from the libraries of each species, identifying a large amount of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microsatellite markers; Penguin; Genome; Polymorphism; Tetranucleotide. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400676 |
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| Teixeira,Santuza M.; Paiva,Rita Márcia Cardoso de; Kangussu-Marcolino,Monica M.; DaRocha,Wanderson D.. |
| In 2005, draft sequences of the genomes of Trypanosoma brucei, Trypanosoma cruzi and Leishmania major, also known as the Tri-Tryp genomes, were published. These protozoan parasites are the causative agents of three distinct insect-borne diseases, namely sleeping sickness, Chagas disease and leishmaniasis, all with a worldwide distribution. Despite the large estimated evolutionary distance among them, a conserved core of ~6,200 trypanosomatid genes was found among the Tri-Tryp genomes. Extensive analysis of these genomic sequences has greatly increased our understanding of the biology of these parasites and their host-parasite interactions. In this article, we review the recent advances in the comparative genomics of these three species. This analysis also... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Trypanosoma brucei; Trypanosoma cruzi; Leishmania major; Genome; RNAseq. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100001 |
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| Santos,Mônica de Oliveira; Castro,Nadya da Silva; Pereira,Maristela; Soares,Célia Maria de Almeida. |
| This is a report on the analysis of genes involved in translation of the complete genomes of Mycoplasma hyopneumoniae strain J and 7448 and Mycoplasma synoviae. In both genomes 31 ORFs encoding large ribosomal subunit proteins and 19 ORFs encoding small ribosomal subunit proteins were found. Ten ribosomal protein gene clusters encoding 42 ribosomal proteins were found in M. synoviae, while 8 clusters encoding 39 ribosomal proteins were found in both M. hyopneumoniae strains. The L33 gene of the M. hyopneumoniae strain 7448 presented two copies in different locations. The genes encoding initiation factors (IF-1, IF-2 and IF-3), elongation factors (EF-G, EF-Tu, EF-Ts and EF-P), and the genes encoding the ribosome recycling factor (frr) and one polypeptide... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycoplasmas; Mycoplasma synoviae; Mycoplasma hyopneumoniae; Translation; Genome. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200010 |
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| Cagliari,Thiago C.; Tiroli,Ana O.; Borges,Júlio C.; Ramos,Carlos H.I.. |
| Expressed Sequence Tags (ESTs) sequencing provides reliable and useful information concerning gene expression patterns in the genomic context. Our group used bioinformatics to identify and annotate 5'EST-contigs belonging to the molecular chaperones within the Eucalyptus Genome Sequencing Project Consortium (FORESTs) database. We found that 1,959 5'EST-contigs, or approximately 1.6% of the total 5'EST-contigs, encoded chaperones, emphasizing their biological importance. About 55% of the chaperones that we found were Hsp70 chaperones and its co-chaperones, 18% were Hsp90 chaperones, 15% were Hsp60 and its co-chaperone, 8% were Hsp100 chaperones, and 4% were Small Hsps. We also investigated the digital expression profile of the chaperone genes to gain... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chaperones; Heat shock proteins; Genome; Expressed sequence tags. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400006 |
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| Peroni,Luis Antonio; Ferreira,Renato Rodrigues; Figueira,Antonio; Machado,Marcos Antonio; Stach-Machado,Dagmar Ruth. |
| Plants not only evolve but also reduce oxygen in photosynthesis. An inevitable consequence of this normal process is the production of reactive oxygen species (ROS). Plants are adequately protected by the presence of multiple antioxidative enzymes in the cytosol and also in the different cell organelles such as chloroplasts, mitochondria, and peroxisomes. Traditionally, ROS were considered to be only a toxic byproduct of aerobic metabolism. However, recently it has become apparent that plants actively produce these molecules which may control many different physiological processes such as abiotic and biotic stress response, pathogen defense and systemic signaling. The search results using the Citrus Genome Program in Brazil (CitEST) for oxidative stress... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Citrus; Genome; EST; Reactive oxygen species; Oxidative stress enzyme. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500016 |
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| Zhang,Hai-Qin; Yang,Rui-Wu; Zhang,Li; Ding,Chun-Bang; Zeng,Jian; Zhou,Yong-Hong. |
| The phylogenetic relationships of 15 taxa from Hystrix and the related genera Leymus (NsXm), Elymus (StH), Pseudoroegneria (St), Hordeum (H), Psathyrostachys (Ns), and Thinopyrum (E) were examined by using the Giemsa C-banded karyotype. The Hy. patula C-banding pattern was similar to those of Elymus species, whereas C-banding patterns of the other Hystrix species were similar to those of Leymus species. The results suggest high genetic diversity within Hystrix, and support treating Hy. patula as E. hystrix L., and transferring Hy. coreana, Hy. duthiei ssp. duthiei and Hy. duthiei ssp. longearistata to the genus Leymus. On comparing C-banding patterns of Elymus species with their diploid ancestors (Pseudoroegneria and Hordeum), there are indications that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: C-banding; Elymus; Genome; Hystrix; Leymus; Triticeae. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300015 |
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| Brandão,José Henrique S. G.; Rodrigues,Nureyev F.; Eguiluz,Maria; Guzman,Frank; Margis,Rogerio. |
| Abstract Araucaria angustifolia is endemic to southern Brazil. Known as Brazilian pine, A. angustifolia is the only native conifer species with economic and social relevance in this country. Due to massive exploitation, it has suffered a significant population decline and currently is classified as critically endangered. This encouraged the scientific community to investigate genetic features in Brazilian pine to increase resources for management and preservation. In this work, RNA-Seq data was used to determine the complete nucleotide sequence of the A. angustifolia chloroplast genome (cpDNA). The cpDNA is 146,203 bp in length and contains 122 genes, including 80 protein-coding genes, 5 ribosomal RNA genes, and 37 tRNA genes. Coding regions comprise... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brazilian pine; Plastid; Genome; CpDNA; Conservation. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000400671 |
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| Carazzolle,Marcelo F.; Formighieri,Eduardo F.; Digiampietri,Luciano A.; Araujo,Marcos R.R.; Costa,Gustavo G.L.; Pereira,Gonçalo A.G.. |
| Genome projects, both genomic DNA and ESTs (cDNA), generate a large amount of information, demanding time and a well-structured bioinformatics laboratory to manage these data. These genome projects use information available in heterogeneous formats from different sources. The amount and heterogeneity of this information, as well as the absence of a world consensus pattern, make the integration of these data a difficult task. At the same time, sub-tasks, such as microarray analyses of these projects, are very complex. This creates a demand for the development of creative solutions for ongoing annotation, thematic projects, microarray experiments, etc. This paper presents Gene Projects, a system developed to integrate all kinds of solutions. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Annotation; Genome; Web; Microarray and clustering. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500032 |
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| Fietto,Luciano G.; Costa,Maximiller D.L.; Cruz,Cosme D.; Souza,Alessandra A.; Machado,Marcos A.; Fontes,Elizabeth P.B.. |
| The completion of the genome sequencing of the Arabidopsis thaliana model system provided a powerful molecular tool for comparative analysis of gene families present in the genome of economically relevant plant species. In this investigation, we used the sequences of the Arabidopsis Hsp70 gene family to identify and annotate the Citrus Hsp70 genes represented in the CitEST database. Based on sequence comparison analysis, we identified 18 clusters that were further divided into 5 subgroups encoding four mitochondrial mtHsp70s, three plastid csHsp70s, one ER luminal Hsp70 BiP, two HSP110/SSE-related proteins and eight cytosolic Hsp/Hsc70s. We also analyzed the expression profile by digital Northern of each Hsp70 transcript in different organs and in response... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chaperones; Digital northern; Heat shock proteins; Genome; Expressed sequence tags. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500017 |
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| Borges,Clayton Luiz; Parente,Juliana Alves; Pereira,Maristela; Soares,Célia Maria de Almeida. |
| Mycoplasmas are the smallest known prokaryotes with self-replication ability. They are obligate parasites, taking up many molecules of their hosts and acting as pathogens in men, animals, birds and plants. Mycoplasma hyopneumoniae is the infective agent of swine mycoplasmosis and Mycoplasma synoviae is responsible for subclinical upper respiratory infections that may result in airsacculitis and synovitis in chickens and turkeys. These highly infectious organisms present a worldwide distribution and are responsible for major economic problems. Proteins of the GTPase superfamily occur in all domains of life, regulating functions such as protein synthesis, cell cycle and differentiation. Despite their functional diversity, all GTPases are believed to have... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycoplasma; GTPase superfamily; Genome. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000200007 |
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| Azevedo,Gustavo P.R.; Paz,Pedro H. C da; Mattsson,Hannah K.; Moreira,Ana Paula B.; Leomil,Luciana; Calegário,Gabriela; Appolinario,Luciana; Vidal,Lívia; Silva,Bruno S.; Tonon,Luciane A. Chimetto; Tschoeke,Diogo A.; Garcia,Gizele D.; Thompson,Fabiano L.; Thompson,Cristiane C.. |
| Abstract We present here the genome sequence of Shewanella corallii strain A687 isolated from pufferfish Sphoeroides spengleri (Family Tetraodontidae). The assembly consists of 5,215,037 bp and contains 284 contigs, with a G+C content of 50.3%. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shewanella; Gammaproteobacteria; Genome; Gene prediction; Secondary metabolism. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100401 |
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| VASCONCELOS, M. J. V. de; FIGUEIREDO, J. E. F.. |
| Nos últimos anos, a tecnologia de edição genômica baseada no uso de nucleases “engenheiradas” ou quiméricas está revolucionando as abordagens de engenharia gênica e ampliando o repertório de animais e plantas geneticamente modificados, o que era impossível de ser obtido com as tecnologias tradicionais de manipulação gênica. O sistema enzimático quimérico CRISPR-Cas é uma poderosa ferramenta que permite realizar modificações genéticas precisas e específicas nas cadeias de DNA ou gerar rearranjos genômicos. Entre as aplicações de CRISPR/Cas, destacam-se as alterações da expressão gênica por meio do silenciamento, repressão, indução e ganho de função; modulação e alteração da atividade de proteínas; introdução de genes exógenos para induzir novas... |
| Tipo: Documentos (INFOTECA-E) |
Palavras-chave: Melhoramento genético.; Genética; Genoma.; Breeding; Genetics; Genome. |
| Ano: 2015 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1039785 |
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| Registros recuperados: 42 | |
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