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Population dynamics of Pseudomonas savastanoi pv. phaseolicola in bean, throughout the epiphytic and pathogenic phases PAB
Marques,Abi Soares dos Anjos; Samson,Régine.
Abstract: The objective of this work was to monitor traits of the life cycle of Pseudomonas savastanoi pv. phaseolicola, in order to better understand the outbreak of bean halo blight, originating from a bacterial population in asymptomatic plants. Five experiments were conducted in the field, in greenhouses, and in humidity chambers. Changes in population size were evaluated in three field plantings, by introducing the bacteria in contamination focal points and observing the weather conditions favoring an outbreak. The dispersion of the bacteria in the field was followed by isolation and Bio-PCR analysis. Two assays were conducted in greenhouses and humidity chambers to evaluate the effect of leaf age on disease expression and the relationship between...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris; Bio-PCR; Epidemiology; Halo blight; Plant quarantine; Weather conditions..
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2016000500623
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Application of semi-selective mediums in routine diagnostic testing of Pseudomonas savastanoi pv. phaseolicola on common bean seeds Scientia Agricola
Popovic,Tatjana; Milovanovic,Predrag; Aleksic,Goran; Gavrilovic,Veljko; Starovic,Mira; Vasic,Mirjana; Balaž,Jelica.
Halo blight, caused by Pseudomonas savastanoi pv. phaseolicola (Psp), is considered to be an important bacterial disease on common bean (Phaseolus vulgaris L.) in Serbia. Use of pathogen-free seeds is one of the most effective control measures against this disease. The aim of this study was to evaluate a detection method for Psp on untreated common bean seeds (23 genotypes) from commercial crops grown within Serbia. Detection of this pathogen was made by plating onto the modified sucrose peptone (MSP) and Milk Tween (MT) semi-selective mediums from soaked whole common bean seed. Colonies growing on the MSP medium were light yellow, convex and shiny, whereas on the MT medium, they were creamy white, flat and circular. The pathogenicity of the obtained...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MSP; MT; ELISA; Nested-PCR; Halo blight; Pathogenicity.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000400005
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