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Registros recuperados: 31 | |
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RUIZ,JULIÁN; JAIME,JAIRO; VERA,VÍCTOR. |
El herpesvirus bovino-1 es un virus de distribución mundial causante de graves pérdidas económicas debidas principalmente a la disminución de la eficiencia y en los indicadores de salud y productividad de cualquier hato ganadero infectado. Luego de la infección inicial del tracto respiratorio de los animales, el virus establece un estado de latencia viral en las neuronas sensoriales del ganglio trigémino y en los centros germinales de las tonsilas faríngeas. Periódicamente, el virus es reactivado y excretado en secreciones a través de las cuales puede infectar a otros animales susceptibles. Durante dicho estado de latencia hay disminución dramática de la expresión de genes virales, llevando solo a la expresión de dos transcritos: El RNA codificado por el... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Herpesvirus; Latencia; Transcritos; ORF. |
Ano: 2008 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2008000100001 |
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Aquino Neto,H.M.; Carvalho,A.U.; Facury Filho,E.J.; Ferreira,P.M.; Barbosa-Stancioli,E.F.; Lobato,Z.I.P.; Alvarenga,M.R.; Serrano,A.L.; Martins,R.A.; Afonso,D.A.F.. |
Relata-se um caso de meningoencefalite causada por Herpesvirus bovino 5 (BoHV-5) heritabilityem uma vaca com cinco anos de idade. O animal manifestou quadro clínico inicial de síndrome medular baixa, caracterizada por incoordenação dos membros pélvicos, sinais estes ainda não descritos para a enfermidade. Dentro de pouco tempo a doença evoluiu para síndrome cerebral, e o óbito ocorreu seis dias após o inicio dos sintomas. Na histopatologia, evidenciou-se meningoencefalite difusa, não supurada, e a confirmação do diagnóstico foi feita por reação em cadeia de polimerase e sequenciamento do segmento parcial da glicoproteína G do vírus. O trabalho confirma a presença do BoHV-5 em Minas Gerais, descreve características clínicas novas para a enfermidade e... |
Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Bovino; Herpesvirus; Meningoencefalite; Neuropatia. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352009000100001 |
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Blume,G.R.; Cardoso,S.P.; Oliveira,M.L.B.; Matiolli,M.P.; Gómez,S.Y.M.; Reis Júnior,J.L.; Sant'Ana,F.J.F.; Martins,N.R.S.. |
ABSTRACT Marek's disease (MD) is a lymphoproliferative disorder caused by Gallid herpesvirus 2 (MDV) that infects mainly domestic gallinaceous birds although wild birds may occasionally be affected. The current report describes the anatomopathological and molecular findings of a case of MD in a white-peafowl (Pavo cristatus). The signs included apathy, hyporexia, and diarrhea. Grossly, 0.5 to 1.5cm in diameter, yellow, soft nodules were observed in the skeletal muscle, lung, kidney, air sacs, small intestine, heart, ovary, ventriculus, and proventriculus. Microscopically, numerous atypical round neoplastic cells were noted. The molecular detection of MDV DNA was implemented to amplify part of the meq gene and products were sequenced for the phylogenetic... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Marek's disease; Lymphoma; Oncogenic virus; MDV; Herpesvirus. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000601602 |
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Carvalho,R.; Passos,L.M.F.; Gouvea,A.M.G.; Resende,M.; Martins,A.S.; Franco,G.C.. |
An ELISA system was applied to study the prevalence of EHV-1 in vaccinated and non-vaccinated Brazilian horses. Serum samples were taken from non-symptomatic pregnant mares, before and after the parturition, and from their neonatal foals. The antigen was prepared from a purified virus fraction (HVE-1 AB1 strain). The ELISA absorbance of 0.3516 was determined as the threshold of negative values. This cut-off level was defined from a selected interval, which determined a sensibility of 100% and a specificity of 94.7%, from a serum collection obtained from pregnant mares and fetuses. In this study, a strong correlation was found between the ELISA and serum neutralization for specific EHV-1 antibody detection. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Horse; Herpesvirus; ELISA. |
Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352000000300003 |
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Antonucci,A.M.; Catroxo,M.H.; Hipolito,M.; Takemoto,R.M.; Melo,N.A.; França,F.M.; Teixeira,P.C.; Ferreira,C.M.. |
Feces are an important viral agent elimination route for infected carrier animals and in aquatic organisms these pathogenic agents can very rapidly propagate due to the habitation environment. The objective of this work is to track viral particles in the intestinal contents of bullfrogs (Lithobates catesbeianus) from five commercial frog farms in the region of Vale do Paraíba, in the State of São Paulo, Brazil, using negative contrast transmission electron microscopy (TEM). The Coronaviridae, Paramyxoviridae, Parvoviridae and Herpesviridae families were observed and photographed in specimens. This work emphasizes the importance of adopting sanitary measures in commercial farms and confirms that observing feces by TEM is an efficient and rapid diagnostic... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amphibians; Herpesvirus; Coronavirus; Paramixovirus; Parvovirus. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000200001 |
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Silva,Débora Scopel e; Castro,Clarissa Caetano de; Silva,Fábio da Silva e; Sant'anna,Voltaire; Vargas,Gilberto D'Avila; Lima,Marcelo de; Fischer,Geferson; Brandelli,Adriano; Motta,Amanda de Souza da; Hübner,Silvia de Oliveira. |
P34 is an antimicrobial peptide produced by a Bacillus sp. strain isolated from the intestinal contents of a fish in the Brazilian Amazon basin with reported antibacterial activity. The aim of this work was to evaluate the peptide P34 for its in vitro antiviral properties against canine adenovirus type 2 (CAV-2), canine coronavirus (CCoV), canine distemper virus (CDV), canine parvovirus type 2 (CPV-2), equine arteritis virus (EAV), equine influenza virus (EIV), feline calicivirus (FCV) and feline herpesvirus type 1 (FHV-1). The results showed that the peptide P34 exhibited antiviral activity against EAV and FHV-1. The peptide P34 inhibited the replication of EAV by 99.9% and FHV-1 by 94.4%. Virucidal activity was detected only against EAV. When P34 and EAV... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial peptides; Antiviral activity; Herpesvirus; Equine viral arteritis. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300043 |
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Mancini,Dalva Assunção Portari; Torres,Rosângela Pavan; Pinto,José Ricardo; Mancini-Filho,Jorge. |
This work aimed to evaluate antiviral properties in antioxidants from spices. Phenolic compounds extracted from rosemary (Rosmarinus officinallis, L) by hot water, had their antioxidant activity determined by spectrophotometry using β carotene/linoleic acid system. The rosemary extract was evaluated by antiviral assay of Herpes Virus type-1 (HSV-1) replication in VERO cells, in the presence or absence of the spice. 10,000 TCID50/mL of the HSV-1 was kept for 3 h at 4º C, with 300 ppm of rosemary extract, and 100 ppm of butyl hydroxyl toluene (BHT). Then, these viruses were inoculated in VERO cells incubated at 37º C in CO2-5 %, for seven days. Daily, they were examined and the end point was based on 100% of CPE in virus control (without antioxidants). The... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Natural antioxidants; Virus replication; Phenolic compounds; Herpesvirus; Rosemary. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000100016 |
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Castro,Clarissa Caetano de; Silva,Débora Scopel e; Costa,Géssica Aracéli; Fischer,Geferson; Vargas,Gilberto D’Avila; Brandelli,Adriano; Lima,Marcelo de; Motta,Amanda de Souza da; Hübner,Silvia de Oliveira. |
ABSTRACT: Previous studies have demonstrated the antimicrobial activity of the peptide P34. In this study, the antiviral potential of P34 and the in vitro mechanism of action were investigated against bovine alphaherpesvirus type 1 (BoHV1). P34 exhibited low toxicity, a high selectivity index (22.9) and a percentage of inhibition of up to 100% in MDBK cells. Results from antiviral assays indicated that P34 did not interact with cell receptors, but it was able to inhibit the viral penetration immediately after pre-adsorption. In addition, BoHV1 growth curve in MDBK cells in the presence of P34 revealed a significant reduction in virus titer only 8h post-infection, also suggesting an important role at late stages of the replicative cycle. Virucidal effect... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antiviral; Herpesvirus; Peptide. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000600451 |
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Kurissio,Jacqueline Kazue; Rodrigues,Marianna Vaz; Taniwaki,Sueli Akemi; Zanutto,Marcelo de Souza; Filoni,Claudia; Galdino,Maicon Vinícius; Araújo Júnior,João Pessoa. |
ABSTRACT: Felis catus gammaherpesvirus 1 (FcaGHV1) may causes an asymptomatic infection that result in an efficient transmission and subsequently dissemination of the virus in feline population. This study used molecular detection by qPCR (quantitative PCR) based on DNA polymerase gene fragment amplification to evaluate the occurrence of FcaGHV1 and its correlation with other feline viral pathogens, such as Carnivore protoparvovirus 1 (CPPV-1), Felid alphaherpesvirus 1 (FeHV-1), and feline retroviruses such as feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV). Of the 182 blood samples evaluated 23.6% (43/182) were positives for FcaGHV1. Approximately 37.9% (33/87) of the samples that tested positive for retrovirus were also were positive... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gammaherpesvirus; Herpesvirus; Feline pathogen; Infectious diseases; Diagnosis. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300451 |
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Pepin, Jean-francois; Riou, Antoine; Renault, Tristan. |
Herpes and herpes-like virus infections have been reported in various marine mollusc species associated with high mortality rates. Following the characterisation and genome sequencing of ostreid herpesvirus 1 (OsHV-1), specific diagnostic tools have been developed based on conventional PCR techniques or in situ hybridisation. We have now developed a real-time PCR assay for rapid, sensitive and quantitative detection of OsHV-1, and compared it with a conventional PCR technique described previously. The new assay utilised SYBR® Green chemistry with specific primers C9/C10 targeting the C region. The melt curve analysis of OsHV-1 DNA or DNA extracted from infected material showed only one melting temperature peak (75.75 ± 0.1 °C). The assay had a detection... |
Tipo: Text |
Palavras-chave: SYBR® Green; Real time PCR; Crassostrea gigas; Ostreid herpesvirus 1; Herpesvirus. |
Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-3951.pdf |
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Arzul, Isabelle; Renault, Tristan; Lipart, Cécile; Davison, Andrew. |
Since 1991, numerous herpesvirus infections associated with high mortality have been reported around the world in various marine bivalve species. In order to determine whether these infections are due to ostreid herpesvirus-l (OsHV1), a previously characterized pathogen of the Japanese oyster (Crassostrea gigas), PCR analysis was carried out on 30 samples of larvae collected from four bivalve species (C. gigas, Ostrea edulis, Ruditapes decussatus and Ruditapes philippinarum), most exhibiting mortality prior to collection. All samples were shown to be infected by OsHV1. Viral genomes in three samples of C. gigas and three of R. philippinarum that originated from the same hatchery were unusual in bearing a deletion of at least 2.8 kbp in an inverted repeat... |
Tipo: Text |
Palavras-chave: Bivalve; Crassostrea gigas; Herpesvirus; Japanese oyster; Transmission. |
Ano: 2001 |
URL: http://archimer.ifremer.fr/doc/2001/publication-705.pdf |
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Le Deuff, Rose-marie. |
Among the marine molluscs, Pacific oyster, Crassostrea gigas, is actually the most reared species in the world. Noticeably in France, where it was imported between 1960 and 1970, and replaced Portuguese oyster, Crassostrea angulata. Intensification of breeding and the associated introductions of new species may be hazardous. Indeed, they might be responsible for the appearance and the dissemination of new diseases in bivalve molluscs. In addition to these observations, the quasi monobreeding of C. gigas in France enhances the necessity to develop research concerning the diagnosis of pathologies affecting this species, namely the diagnosis of viral diseases. Several families of viruses have been described within marine bivalves molluscs. The purpose of this... |
Tipo: Text |
Palavras-chave: Mollusques bivalves; Crassostrea gigas; Huître japonaise; Pathologie; Iridovirus; Herpèsvirus; Diagnostic; Bivalve molluscs; Crassostrea gigas; Pacific oyster; Pathology; Iridovirus; Herpesvirus; Diagnosis. |
Ano: 1995 |
URL: http://archimer.ifremer.fr/doc/00107/21792/19367.pdf |
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Saulnier, Denis; Reynaud, Yann; Arzul, Isabelle; Miossec, Laurence; Le Roux, Frédérique; Goarant, Cyrille. |
According to the world organisation for animal health (OIE) an emerging disease is defined as a recently admitted serious illness, whose aetiology can, or not, have already been established, and which is likely to be propagated within a population or between populations, for example at the time of international exchanges of aquatic animals and/or products of aquatic animals. Even though the emerging diseases that affect human health have been much studied, those which affect marine organisms and species of aquaculture interest in particular are poorly documented. By restricting emergence to only infectious diseases, we aim at presenting in a non-exhaustive way some scenarios of the emergence of the diseases of aquacultured species by illustrating them with... |
Tipo: Text |
Palavras-chave: Cyprinus carpio; Ostrea edulis; Bonamia ostreae; Peneides; Vibrio nigripulchritudo; Herpesvirus; Agent pathogène. |
Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2980.pdf |
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Arzul, Isabelle; Renault, Tristan; Thebault, Anne; Gerard, Andre. |
Since 1972, several herpes-like virus infections have been reported among different bivalve species around the world. Most of these reports involved larvae or juveniles presenting high mortalities. Two case reports of herpes-like viruses concerned adult oysters, Crassostrea virginica in USA and Ostrea angasi in Australia. Molecular techniques including PCR and in situ hybridization (ISH) have been recently developed to detect the oyster herpesvirus genome. In the present study, 30 Pacific oyster, Crassostrea gigas, adults have been analyzed using three different techniques: PCR, ISH and immunochemistry, in order to detect herpesviruses in asymptomatic individuals. PCR and ISH allowed detection of oyster herpesvirus DNA in 93.3 and 86.6%, respectively, of... |
Tipo: Text |
Palavras-chave: Protein; DNA; Healthy carrier; Adult oyster; Crassostrea gigas; Herpesvirus. |
Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-697.pdf |
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Thebault, Anne; Cochennec, Nathalie; Arzul, Isabelle; Renault, Tristan. |
In marine molluscan aqauculture, infectious agents are regularly cited in literature associated with mass mortalities as emergin diseases, but the causal link is often based on very few argumeents. 24 ways to demonstrate causal links from epidemiolgical, ecological and marine pathology literature feasible for marine molluscs are given. Those criteria were applied to the ewample of Bonamia ostreae for Ostrea edulis and Herpesvirus for spats and larvae of Crassostrea gigas. Three principles could be applied in marine field for choosing criteria wich should be taken in priority : the demonstration must be given at three levels of organization, organ individual, population, the demonstration must keeps maximum epidemiological criterias, and then mixes... |
Tipo: Text |
Palavras-chave: Herpesvirus; Bonamia ostrea; Crassostrea gigas; Ostrea edulis; Marine pathology; Ecology; Epidemiology; Infectious agents; Molluscan aquaculture. |
Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/acte-3341.pdf |
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Corporeau, Charlotte; Tamayo, David; Pernet, Fabrice; Quere, Claudie; Madec, Stephanie. |
Pacific oyster Crassostrea gigas were inoculated with OsHV-1 at low load (control) or high load (challenged) to better understand the pathogenesis of ostreid herpesvirus 1 (OsHV-1 μVar) and to determine which metabolic pathways might be affected during infection. Animals were sampled for proteomic analysis two days post-injection, at the same time as OsHV-1 initiated an intense replication phase in challenged oysters. Twenty-five abundant protein spots that showed a marked change in accumulated levels were identified using a two-dimensional electrophoresis (2-DE) proteomic approach. Overall, these proteins are involved in cytoskeleton organization, protein turnover, induction of stress signals, signalling pathways and energy metabolism. Challenged oysters... |
Tipo: Text |
Palavras-chave: Marine bivalves; Crassostrea gigas; Herpesvirus; Metabolism; Warburg. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00199/31035/29439.pdf |
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Registros recuperados: 31 | |
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