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Dynamique et enzymologie de la digestion chez l'huitre Crassostrea gigas ArchiMer
Boucaud, C; Lebesnerais, C; Lubet, P; Lihrmann, I.
Feeding experiments carried out on Crassostrea gigas allowed the authors to set up the stages and the timing of the movement of food throughout the digestive tract. Various activities involved in digestion (glycosidases, esterases, and peptidases) were investigated histochemically in the digestive tract in order to localize the sites of absorption and the sites of protein and carbohydrate digestion. An explicative model of the different stages of digestion is proposed.
Tipo: Text Palavras-chave: Crassostrea gigas; Bivalvia; Cytology; Histochemistry; Histology; Digestive system; Enzymatic activity; Digestive glands; Digestion; Enzymes; Transit digestif; Digestion; Huitre; Crassostrea gigas.
Ano: 1983 URL: http://archimer.ifremer.fr/doc/1983/acte-1179.pdf
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Trophic relationships gonad-intestinal loop in Pecten maximus . ArchiMer
Lepennec, M; Dorange, G; Beninger, P; Donval, A; Widowati, I.
Histological, histochemical and ultrastructural studies were performed on the region of the gonad traversed by the intestine in Pecten maximus . The results indicate a digestive and absorptive function of intestine epithelial cells and a transfer of metabolites to the surrounding gonad tissue. Several possible mechanisms of translocation are presented, including diffusion into the haemolymph and transfer via vacuolated cells of the gonad interstitial connective tissue.
Tipo: Text Palavras-chave: Pecten maximus; Bivalvia; Histology; Haemolymph; Histochemistry; Cells; Metabolites; Epithelia; Intestines; Ultrastructure; Animal reproductive organs.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-1668.pdf
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Localisation immunohisto chimique de peptides apparentes aux hormones de vertebres chez Pecten maximus ArchiMer
Donval, A.
Substances related to vertebrate peptidic hormones have been searched by immunohistochemical methods in Pecten maximus . Neurosecretory cells have been detected in the nervous ganglia with antisera raised against vertebrate hormones, gastrin/CCK like, glucagon like, insuline like, somatostatine like. Other gastro-intestinal hormones like are localized in some cells of the gut epithelium: bombesine and vasoactive intestinal peptide.
Tipo: Text Palavras-chave: Pecten maximus; Bivalvia; Immunology; Histochemistry; Cells; Neurosecretory system; Juveniles; Hormones.
Ano: 1991 URL: http://archimer.ifremer.fr/doc/1991/acte-1724.pdf
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Localization of Sugar Residues in the Stomach of Three Species of Monkeys (Tupaiidae glis, Nycticebus cocang and Callithrix jacchus) by Lectin Histochemistry International Journal of Morphology
Fayed,Masoud Hassan; Elnasharty,Mohamed; Shoaib,Mahmoud.
The stomach of three species of non-human primates was investigated by lectin histochemistry to clarify the staining affinity and distribution patterns of their sugar residues. All gastric regions, with little differences between the deep and superficial parts of the same region, were rich in. in N-acetylglucosamine and/or neuraminic acid. Although, the superficial regions of the gastric mucosa were scanty in N-acetylgalactosamine, a- D-glucose and a -D-mannose, the deep parts of the gastric mucosa were rich in these sugars. In conclusion, there is a difference among the mucosubstances of surface and foveolar mucous cells, mucous neck cells, and gastric gland cells. This indicates heterogeneous composition of gastric mucus, or mucus molecules with...
Tipo: Journal article Palavras-chave: Histochemistry; Lectins; Non-human primates; Stomach.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022010000100016
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Clinical and Histochemical Alterations of the Periodontal Ligament in Gerbils after Malocclusion Induced International Journal of Morphology
Naves,Leandro Moura Leite; Issa,João Paulo Mardegan; Pitol,Dimitrius Leonardo; Fukada,Sandra Yasuyo; di Matteo,Miguel Ángel Sala; Iyomasa,Mamie Mizusaki.
The aim of this article is to show the clinical and histochemical alterations of the first periodontal ligament, on the right side, after upper molars teeth extraction on the left side in gerbils. After two months, the periodontal ligaments were removed and processed for histochemical analysis. The data showed that TRAP reaction was able to evidence the osteoclastic activity in the hyperfunction hemimandible, right side, explaining the functional changes in the periodontal ligament after teeth extraction, and a little gingival recession and radicular exposure of teeth without function was observed at inferior molars of the left side
Tipo: Journal article Palavras-chave: Periodontal ligament; Histochemistry; Malocclusion; Gerbil; Meriones unguiculatus.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000400036
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Histochemical Features of the Extracellular Matrix in Rattus rattus norvegicus Otic Ganglion International Journal of Morphology
Chagas,M. A.; Babinski,M. A.; Abidu-Figueiredo,M.; Costa,W. S..
The otic ganglion is a cranial component of the parasympathetic division of the autonomic nervous system (ANS). Similar to other parasympathetic ganglia, otic ganglion presents multipolar neurons that are completely surrounded by satellite cells and intercellular substance as well, which allow us to use this ganglion as a good experimental model for studying the relationship neuron / extracellular matrix. We have studied rat otic ganglion in 10 animals through light microscopy. After routine histological methods, 5 µm sections were obtained and coloured by Gomori`s trichrome, periodic acid-Schiff (PAS), Alcian Blue pH 2.5 and pH1.0, acetylation + PAS, acetylation + deacetylation + PAS, acid hydrolysis + PAS, phenylhydrazine + PAS and thiosemicarbazide +...
Tipo: Journal article Palavras-chave: Rat; Otic ganglion; Autonomic nervous system; Histochemistry.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000100015
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Histological and Histochemical Changes by Clove Essential Oil Upon the Gonads of Spodoptera frugiperda (JE Smith) (Lepidoptera: Noctuidae) International Journal of Morphology
Cruz,Glaucilane dos Santos; Teixeira,Valeria Wanderley; de Oliveira,Jose Vargas; Teixeira,Alvaro Aguiar Coelho; Araújo,Alicely Correia; Alves,Thiago Jose de Souza; da Cunha,Franklin Magliano; Breda,Mariana Oliveira.
Spodoptera frugiperda is a polyphagous insect that causes economic losses to several crops in Brazil and is the major obstacle to corn production. Researches focusing on alternative control, e.g. botanical products are expanding to offer a wide variety of molecules that interfere with different biological parameters of insect pests. Thus, this study tested the hypothesis that clove essential oil affects the spermatogenesis, ovarioles histochemistry and the fertility of S. frugiperda. The results showed that clove essential oil affects the gametogenesis of S. frugiperda ovarioles, reflecting negatively on its reproduction, proving to be a promising tool for controlling this pest.
Tipo: Journal article Palavras-chave: Fall armyworm; Spodoptera frugiperda; Reproduction; Histochemistry; Histology; Syzygium aromaticum.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000400034
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Influence of Reduced Salinity on the Histochemical Fiber Type Composition of the Claw Closer Muscle of Two Estuarine Crab Species, Cyrtograpsus angulatus and Neohelice granulata (Decapoda: Varunidae) International Journal of Morphology
Longo,María Victoria; Díaz,Alcira Ofelia.
The estuarine crustaceans are exposed to frequent and abrupt environmental salinity changes that involve adjustments at different levels in muscle tissue. The histochemical composition of fiber types of the claw closer muscle of Cyrtograpsus angulatus and Neohelice granulata (Dana, 1851) under hyper regulatory conditions was analyzed. Cryosections of the muscle were treated with myosin-adenosine triphosphatase (m-ATPase), succinic dehydrogenase (SDH), periodic acid Schiff (PAS) and Sudan Black B. The mean diameters, the relative area and the proportion of each muscle fiber type were calculated. Types I and IV would belong to 'extreme' groups, whereas types II and III would be considered 'intermediate'. Type I fibers were large and exhibited a weak reaction...
Tipo: Journal article Palavras-chave: Euryhaline crabs; Histochemistry; Hyper regulation; Muscle fibers; Salinity stress.
Ano: 2017 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000200025
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Lymphoglandular Complexes in Proximal Colon of Buffalo Calves (Bubalus bubalis) International Journal of Morphology
Kapoor,Kritima; Singh,Opinder.
The present study was conducted on six healthy early neonatal and six prepubertal buffalo calves to study the location, gross morphology, histomorphology and histochemistry of lymphoglandular complexes in proximal colon. In very proximal part of colon of buffalo calves, an irregular oval mucosal lymphoid patch was found grossly as a proximal colon (PC) patch. Histologically, in proximal colon patch of early neonates (3-4 weeks), an extensive invasion of mucosal glands was observed towards lymphoid nodules that were present in submucosa. The structure as a whole thus formed a complex known as lymphoglandular complex (LGC). Large number of such complexes i.e., LGCs were observed in submucosa of proximal colon at this age. At some places, invasion of mucosal...
Tipo: Journal article Palavras-chave: Proximal colon; Lymphoglandular complex; Histomorphology; Histochemistry; Buffalo calves.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000300052
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Histological and Histochemical Studies of the Aorta and Pulmonary Trunk in STZ-induced Diabetic Wistar Rats Treated with Momordica charantia International Journal of Morphology
Komolafe,O. A; Ofusori,D. A; Adewole,O. S; Ayoka,A. O; Bejide,R.
Diabetes mellitus (DM) is a serious metabolic disorder with micro and macro-vascular complications that result in a significant morbidity and mortality. The present study investigated the effects of Momordica charantia (M. charantia) on histological changes of the aorta and pulmonary trunk in streptozotocin-induced diabetic Wistar rats. Forty healthy adult Wistar rats of both sexes were randomly assigned into five groups A, B, C, D and E of eight rats each. Group A were the control (normal rats); B were the experimentally-induced diabetic rats; C were diabetic rats treated with methanolic extracts of M. charantia for two weeks (withdrawal group); D were diabetic rats treated with methanolic extracts of M. charantia for four weeks. E was diabetic rats...
Tipo: Journal article Palavras-chave: Diabetes; Momordica charantia; Histological; Histochemistry; Aorta; Pulmonary trunk.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200060
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Age Related Structural Changes in the Bulbourethral Gland of Gaddi Goat (Capra hircus) International Journal of Morphology
Archana,P; Katiyar,R. S; Sharma,D. N; Farooqui,M. M; Prakash,Ajay.
A study was conducted on the bulbourethral glands of 30 Gaddi goats, divided into three groups of 10 animals in each viz; Prepubertal (1 day old to < 18 months of age), Pubertal (18 months to < 5yrs of age) and Postpubertal (>5yrs of age). The study revealed that the glands grew at a faster speed up-to 6 months of postnatal life after which the pace slowed down considerably. Almost 10 times increase in weight and more than three times increase in all the linear mensurations were observed in prepubertal group from the time of birth. The stroma vs parenchyma ratio which was 80:20 in one-day-old kids showed three times increase in parenchyma in prepubertal animals, and the ratio became 43:57. In one day old kids luminated and non-luminated secretory...
Tipo: Journal article Palavras-chave: Bulbourethral gland; Gaddi goat; Histology; Histochemistry.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200049
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Image Analysis of Oxidative and Glycolytic Muscle Fibers During Reperfusion Injury by Segmentation Based on Regions International Journal of Morphology
Rosero-Salazar,Doris Haydee.
Different situations cause ischemia and reperfusion injury, affecting tissues under the level of compression. In this research, abnormal characteristics in distribution of muscle fibers types in soleus and extensor carpi radialis longus, during short periods of ischemia and short and long periods of reperfusion, were determined. Fibers were classified by enzyme histochemistry techniques NADH-TR and myosin-ATPase. Measurements of areas were carried out through semiautomatic image processing by using segmentation based on regions, which evidenced significant changes in distribution during reperfusion followed to one and three hours of ischemia, as well as in comparisons of areas for all periods of reperfusion. This study strengthens the evidence about using...
Tipo: Journal article Palavras-chave: Histochemistry; Ischemia; Reperfusion; Image analysis; Segmentation; Histoquímica; Isquemia; Reperfusión; Análisis de imágenes; Segmentación.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000100019
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Mucous Membrane of the Rumen of Ovines, Fed With Spineless, Forrage Cactus or Palm (Barbary Fig) (Opuntia ficus indica Mil): Hystochemical Study by Means of Light Microscopy International Journal of Morphology
Neiva,Gentileza Santos Martins; da Mota,Diógenes Luís; Batista,Ângela Maria Vieira; Sousa-Rodrigues,Célio Fernando de.
The mucosa of the rumen performs the functions of absorption, transportation, metabolism and protection, due to the presence of microorganisms in this compartment of the digestive tract, which are responsible for the digestion of approximately 60% of the organic material apparently digested. The histological structure and the histochemical profile of glicoconjugate of the mucosa of the stomach of sheep that were being fed with forrage cactus or palm (Giant Palm - Opuntia ficus indica Mil)1. Twelve sheep were used, with approximately 14 months old, which were oddly distributed in four diets: D1- 60.0% forrage palm + 40.0% concentrated ration; D2- 47.5% forrage palm + 12.5% elephant, napier grass + 40.0% concentrated ration, and D3 - 34,8% forrage palm +...
Tipo: Journal article Palavras-chave: Mucous membrane; Histology; Histochemistry; Forrage palm; Forrage cactus; Ovine.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500034
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Preliminary Study of the Sonic Muscle of Micropogonias furnieri (Actinopterygii, Sciaenidae): Morfología e Histoquímica International Journal of Morphology
Devincenti,Clelia Viviana; Díaz,Alcira Ofelia; Longo,María Victoria; García,Alicia Mabel; Figueroa,Daniel; Goldemberg,Adriana Lía.
The present study highlights the morphology of the Micropogonias furnieri sonic muscle by means of histochemical techniques, relating it to previous histochemical studies of the pectoral fin and myotomal muscles. In order to classify the muscle fibres, succinic dehydrogenase (SDH) for mitochondria, periodic acid Schiff (PAS) for glycogen, Sudan Black and Red for lipids and myosin adenosine triphosphatase (m-ATPase) pre-incubated at alkaline and acid pHs to visualize the contraction velocity, were used. The sonic fibres were smaller than the white myotomal and pectoral fibres, showed homogenous size and distribution and had features common to white fibres: negative to SDH and lipids, weakly positive to PAS and m-ATPase following acid pre-incubation, and...
Tipo: Journal article Palavras-chave: Sonic muscle; Micropogonias furnieri; Morphology; Histochemistry.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400027
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Morphometry and Histochemistry of the Rectus abdominis Muscle Fibers of Tufted Capuchin Monkeys (Cebus apella Linnaeus, 1758) International Journal of Morphology
Simionato,L. H.; Andreo,J. C.; Oliveira,J. A. de; Bortoluci,C. H. F.; dos Santos,N. B.; Moraes,L. H. R.; Rodrigues,A. C.; Andreo,M. B..
The phylogenetic proximity of primates to humans, along with their behavioral, biochemical, and anatomical similarities, make such animals more interesting experimental models for biomedical researches, as compared to classical laboratory animals. Another aspect that has called the attention of researchers is the differentiated quadrupedalism present in some primates. The tufted capuchin monkey uses the ground and tree branches as its support for locomotion, showing various postures while performing this task. On the basis of this information, we have decided to study the rectus abdominis muscle of the tufted capuchin monkey, with the following goals: the frequency and area of fiber types; its possible compartmentalization; and identify if this muscle is...
Tipo: Journal article Palavras-chave: Rectus abdominis muscle; Histochemistry; Musculars fiber types; Tufted capuchin monkey; Cebus apella; Primates; Simians.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000100011
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Comparative Intestinal Histomorphology of Five Species of Phyllostomid Bats (Phyllostomidae, Microchiroptera): Ecomorphological Relations with Alimentary Habits International Journal of Morphology
Gadelha-Alves,Rafaela; Rozensztranch,Andréa Melo Da Silva; Rocha- Barbosa,Oscar.
The intestines of Sturnira lilium, Phyllostomus hastatus, Carolliaperspicillata, GlossophagasoricinaíindDesmodus rotundus were analized through macroscopic, morphometric and light microscopy studies. The species studied presented some common charactenstics generally found in bats, such as the absence of a caecum and an appendix. The frugivorous bat S. lilium has a longer intestine than the other species. The intestinal epithelium is of the simple columnar type, constituted of absorptive cells characterized by microvilli and goblet cells, which in all the species proved to be PAS + and AB +. These cells presented some differences in quantity and in distribution among the species. The intestinal mucosa presented morphologic and morphometric differences, such...
Tipo: Journal article Palavras-chave: Intestines; Bats; Phyllostomidae; Histology; Histochemistry; Micrometry; Ecomorphology.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022008000300014
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Androgen Receptor Distribution, PAS and Alcyan Blue Reaction in the Vomeronasal Organ and the Nasal Septum Mucosa of the Developing Male Rat International Journal of Morphology
Núñez Chichet,M E; Genovese,P; Bielli,A.
The vomeronasal organ (VNO) is a tubular chemoreceptory organ which detects environmental pheromones and is essential for mammalian normal reproductive activity. A sensitive chemoreceptory neuroepithelium lines the concave VNO wall and the opposite, convex wall is lined by a seudostratified, receptor-free epithelium. The secretion of the Jacobson glands (JG) -tubuloacinary glands lying in the lamina propia of the VNO - is essential for pheromones contacting vomeronasal organ chemoreceptors. Tubuloacinary glands lying in the connective tissue of the nasal septum mucosa (Bowman glands), can be classified as ventral and dorsal, according to their location. Positivity to PAS and Alcyan blue reactions and androgen receptor immunolocalization was evaluated with...
Tipo: Journal article Palavras-chave: Vomeronasal organ; Chemoreception; Development; Histochemistry; Immunohistochemistry.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300018
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Histopathology of Organs from Neonate Offspring Born to Female Rats Exposed to Sublethal Doses of Biological and Synthetic Insecticides International Journal of Morphology
dos Santos,Hilda Michelly Paiva; Teixeira,Valéria Wanderley; Teixeira,Álvaro Aguiar Coelho; da Silva,Rosimere; Lemos,Ana Janaina Jeanine Martins; Neto,Clovis José Cavalcanti Lapa; Ferreira,Cintia Giselle Martins.
The current annual consumption of insecticides in agriculture in Brazil is over three million tons. The exposure happens through food that is contaminated with toxic waste and respiratory and dermal absorption. Biological control with Bacillus thuringiensis Berliner (Bt) has assumed increasing importance replacing the use of conventional insecticides. However, the tests used to justify approval for use and market release of Bt products, and the protocols used to assess the development of toxicity, are extremely superficial. In addition, more studies comparing effects between males and females and during pregnancy and lactation have not been conducted. Thus, the hypothesis tested in this study was if the administration of the XenTari® WG (Bacillus...
Tipo: Journal article Palavras-chave: Bacillus thuringiensis; Synthetic insecticide; Rats; Histology; Histochemistry.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100040
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Histology and histochemistry of the venom apparatus of the black scorpion Androctonus crassicauda (Olivier, 1807) (Scorpiones: Buthidae) J. Venom. Anim. Toxins incl. Trop. Dis.
Jarrar,BM; Al-Rowaily,MA.
The venom apparatus of the black scorpion Androctonus crassicauda has been characterized histologically and histochemically in the present study. The results showed that this apparatus consists of paired venom glands, each of which initially presents its own canal and posteriorily both fuse into a single common one. Each gland is covered by a sheath of striated muscle and is lined with extensively folded secretory epithelium (formed of non-secretory and secretory venom-producing cells). The outcomes also revealed that the venom-producing cells of both glands produce neutral mucosubstances, sialomucins, sulfomucins and proteins, but are devoid of glycogen. Cysteine, tyrosine, tryptophan and arginine were also detected along with activities of acid and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Androctonus; Scorpion; Venom apparatus; Histochemistry; Phosphatases; Esterases; Peptidases.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992008000300010
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Ultramorphological analysis of the venom glands and their histochemical relationship with the convoluted glands in the primitive social paper wasp Polistes versicolor (Hymenoptera: Vespidae) J. Venom. Anim. Toxins incl. Trop. Dis.
Britto,F. B.; Caetano,F. H..
The venom glands are part of the most important defense weapon in Aculeata: the venom apparatus. The arrangement of these glands can vary among species, but in general they are composed of long secretory tubules connected to a muscular sac-like reservoir. Although the occurrence of these variations has been documented, many studies neglected the existence of a well-developed secretory portion in the lumen of the reservoir named convoluted gland. This study is an ultramorphological analysis of the venom glands and their histochemical relationship with the convoluted glands in the primitive social wasp Polistes versicolor. In this wasp, the venom glands are constituted by two tubular portions that penetrate individually in the venom reservoir, inside of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wasps; Venom glands; Convoluted glands; Ultramorphology; Histochemistry.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000200007
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