SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

High genetic diversity and small genetic variation among populations of Magnolia wufengensis (Magnoliaceae), revealed by ISSR and SRAP marker

Hakki,Erdogan E; Kayis,Seyit A; Pinarkara,Emine; Sag,Ayla.

Cannabis sativa L. is a multiple-use plant that provides raw material for the production of seed oil, natural fiber for textiles, automotive and pulp industries. It has also been used in insulating boards, ropes, varnishes, animal feed, and as medicinal agents. Cannabis has potential to be used for phytoremediation: however, its cultivation is strictly controlled due to its psychoactive nature and usage in producing drugs such as marijuana, and hashish. In this study, psychoactive type Cannabis samples, which were seized from 23 different locations of Turkey, and nine hemp type Cannabis accessions, as well as an unknown accession were used. Our interest was to identify the genetic relatedness of the seized samples and to separate drug and hemp type plants....

Tipo: Journal article

Palavras-chave: Cluster analysis; Hemp; ISSR; Marijuana; Principle coordinate analysis.

Ano: 2007

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400010

Greenhouse evaluation on the performance of heat tolerant transgenic broccoli and genetic diversity analysis using inter simple sequence repeat (ISSR) markers

Chen,Liyuan; Chen,Faju; He,Suichao; Ma,Luyi.

Background Genetic diversity and genetic variation of 10 populations and subpopulations of Magnolia wufengensis, a new and endangered endemic species, were examined by inter simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) molecular markers. Compared with other endangered endemic Magnolia taxa, M. wufengensis holds a relatively high level of genetic variation. Result Total genetic diversity was found to be 87.7% for ISSR and 88.0% for SRAP markers. For polymorphic loci (P), the effective mean number of alleles (Ae) was 1.414 for ISSR markers and 1.458 for SRAP markers, while the mean expected heterozygosity (H) was 0.256 using ISSR and 0.291 for SRAP markers. Within-population variation was estimated for P as 74.9% using...

Tipo: Journal article

Palavras-chave: Genetic structure; ISSR; Population genetic diversity; SRAP.

Ano: 2014

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600003

Ravanfar,Seyed Ali; Aziz,Maheran Abdul; Shabanimofrad,Mahmoodreza; Samarfard,Samira.

Background: Broccoli, Brassica oleracea subsp. italica is one of the many valuable Brassica species which is still less cultured under in vitro condition. Heat tolerant transgenic and non-transgenic broccoli cv. Green Marvel plantlets with well-developed root system obtained through in vitro culture were transferred into disposable plastic pots containing sterilized potting mixture consisting of (peatgroTM) + coconut dust (2:1) and maintained in a growth chamber. Results: After one month, the hardened plantlets were transferred and maintained in a transgenic greenhouse. After four months of acclimatization in the transgenic greenhouse, the efficacy of HSP101 gene in increasing the heat tolerance of the transgenic broccoli was evaluated. Results showed that...

Tipo: Journal article

Palavras-chave: AtHSP101 gene; Brassica oleracea; ISSR; Transgenic broccoli.

Ano: 2013

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000500004

Construction of a molecular identification profile of new varieties of Nierembergia linariaefolia by anchored microsatellites

Pérez de la Torre,Mariana C.; Escandón,Alejandro S..

The objective of the present work was to establish the molecular identification profile for six new varieties of Nierembergia linariaefolia to incorporate the fingerprint, as complementary information to the standard registration data. Total DNA was extracted from young leaves following the protocol of the cetyltrimethylammonium bromide. Anchored microsatellites were used as molecular markers. The amplification reactions were carried out with seven primers. A total of 251 loci were detected, 98% of them were polymorphic. The average of polymorphic loci was 35 loci per primer and, 41 loci per genotype. Six out of the seven primers used discriminated all the individuals involved in the present study; consequently, it was possible to generate the molecular...

Tipo: Journal article

Palavras-chave: ISSR; Ornamentals; Varietal identification.

Ano: 2006

URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300014

Clonal diversity and conservation genetics of the medicinal plant Carapichea ipecacuanha (Rubiaceae)

Oliveira,Luiz Orlando de; Venturini,Bruna Afonso; Rossi,Ana Aparecida Bandini; Hastenreiter,Saulo Santos.

The roots of the understorey shrub Carapichea ipecacuanha (ipecac) have medicinal properties, and the uprooting of wild plants has supplied most of the world demand for this species. Although under severe population decline, C. ipecacuanha lacks legal protection. In the wild, the aerial stems of ipecac clump together to form clusters with well-defined borders. Cluster size may range from several to hundreds of aerial stems. To investigate the extent of clonality among aerial stems in ipecac clusters, we sampled 50 wild clusters (a total of 291 aerial stems) and screened them with 89 inter-simple sequence repeat (ISSR) markers. The 291 aerial stems were grouped into 42 putative clones. The clonal groups generally consisted of aerial stems from the same...

Genetic diversity and structure of Atta robusta (Hymenoptera, Formicidae, Attini), an endangered species endemic to the restinga ecoregion

Palavras-chave: Clonal propagation; Genetic diversity; ISSR; Poaia; Psychotria ipecacuanha.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100016

Reis,Evelyze Pinheiro dos; Salomão,Tânia Maria Fernandes; Campos,Lucio Antonio de Oliveira; Tavares,Mara Garcia.

The genetic diversity and structure of the ant Atta robusta were assessed by ISSR (inter-simple sequence repeats) in 72 colonies collected from 10 localities in the Brazilian states of Espírito Santo (48 colonies) and Rio de Janeiro (24 colonies). The ISSR pattern included 67 bands, 51 of them (76.1%) polymorphic. Analysis of molecular variance (AMOVA) revealed a high level (57.4%) of inter-population variation, which suggested a high degree of genetic structure that was confirmed by UPGMA (unweighted pair-group method using an arithmetic average) cluster analysis. The significant correlation between genetic and geographic distances (r = 0.64, p < 0.05) indicated isolation that reflected the distance between locations. Overall, the populations were...

Determination of genetic variation in Rhodiola crenulata from the Hengduan Mountains Region, China using inter-simple sequence repeats

Palavras-chave: Endemism; Endangered; ISSR; Population genetics; Restinga.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400015

Lei,Yidong; Gao,Hong; Tsering,Tashi; Shi,Suhua; Zhong,Yang.

The plant Rhodiola crenulata is a perennial herbaceous species distributed in the plateau region of southwestern China, especially the Hengduan Mountains region. It has been one of the most important traditional herbal remedies in Tibet for more than one thousand years, but the accelerated and uncontrolled collection of this plant since the 1980s has lead to deforestation. We used inter-simple sequence repeats (ISSR) to assess levels of genetic variation in R. crenulata from nine diverse natural populations in eastern Tibet and northern Yunnan, the first time such a study has been carried out. The 12 primers we used were able to detect 184 polymorphic loc. Analysis of molecular variance (AMOVA) indicated that species level genetic diversity was relatively...

Genetic variation in cultivated Rheum tanguticum populations

Palavras-chave: Genetic diversity; Hengduan Mountains region; ISSR; Rhodiola crenulata.

Ano: 2006

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200023

Hu,Yanping; Xie,Xiaolong; Wang,Li; Zhang,Huaigang; Yang,Jian; Li,Yi.

To examine whether cultivation reduced genetic variation in the important Chinese medicinal plant Rheum tanguticum, the levels and distribution of genetic variation were investigated using ISSR markers. Fifty-eight R. tanguticum individuals from five cultivated populations were studied. Thirteen primers were used and a total of 320 DNA bands were scored. High levels of genetic diversity were detected in cultivated R. tanguticum (PPB = 82.19, H = 0.2498, H B = 0.3231, I = 0.3812) and could be explained by the outcrossing system, as well as long-lived and human-mediated seed exchanges. Analysis of molecular variance (AMOVA) showed that more genetic variation was found within populations (76.1%) than among them (23.9%). This was supported by the coefficient...

Genetic relationship in Coffea species and parentage determination of interspecific hybrids using ISSR (Inter- Simple Sequence Repeat) markers

Palavras-chave: Cultivated populations; Genetic variation; ISSR; Polygonaceae; Rheum tanguticum Maxim. ex Balf.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400010

Ruas,Paulo M.; Ruas,Claudete F.; Rampim,Leandro; Carvalho,Valdemar P.; Ruas,Eduardo A.; Sera,Tumoru.

Inter-simple sequence repeat (ISSR) markers were used to evaluate genetic divergence among eight Coffea species and to identify the parentage of six interspecific hybrids. A total of 14 primers which contained different simple sequence repeats (SSR) were used as single primers or combined in pairs and tested for PCR amplifications. Two hundred and thirty highly reproducible fragments were amplified, which were then used to estimate the genetic similarity and to cluster the Coffea species and hybrids. High levels of interspecific genetic variation were revealed. The dinucleotide motif (GA)9T combined with other di- tri- and tetra-nucleotides produced a greater number of DNA fragments, mostly polymorphics, suggesting a high frequency of the poly GA...

Development of sequence characterized amplified region markers from intersimple sequence repeat fingerprints for the molecular detection of toxic phytoplankton Alexandrium catenella (Dinophyceae) and pseudo-Nitzschia pseudodelicatissima (Bacillariophyceae) from French coastal waters

Palavras-chave: Coffea; Coffee breeding; DNA markers; ISSR.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300017

ArchiMer

Bornet, Benjamin; Antoine, Elisabeth; Francoise, Sylvaine; Marcaillou-le Baut, Claire.

Harmful algal blooms are a serious threat to shellfish farming and human health all over the world. The monitoring of harmful algae in coastal waters originally involved morphological identification through microscopic examinations, which was often difficult unless performed by specialists and even then often did not permit identification of toxic species. More recently, specific molecular markers have been used to identify specific phytoplankton species or strains. Here we report on the use of the intersimple sequence repeat (ISSR) technique to develop specific sequence characterized amplified region markers (SCAR) and to identify with these tools two toxic species in French coastal waters, the diatom Pseudo-nitzschia pseudodelicatissima (Hasle) Hasle and...

Tipo: Text

Palavras-chave: SCAR; Pseudo nitzschia; Monitoring; Molecular identification; ISSR; Alexandrium.

Ano: 2005

URL: http://archimer.ifremer.fr/doc/2005/publication-780.pdf

ISSR as new markers for genetic characterization and evaluation of relationships among phytoplankton

ArchiMer

Bornet, Benjamin; Antoine, Elisabeth; Bardouil, Michele; Marcaillou-le Baut, Claire.

In order to increase the molecular tools and markers needed for the identification of phytoplankton species, the inter simple sequence repeat ( ISSR) fingerprinting was adapted to micro-algae and its use in genetic analysis was demonstrated. Twelve strains, 6 Alexandrium, 4 Pseudo-nitzschia, 1 Skeletonema and 1 Tetraselmis were analysed for the first time with ISSR amplifications. The patterns were highly polymorphic and very reproducible. The 6 primers gave 223 polymorphic markers that clearly and easily distinguished all 12 strains ( mainly toxic ones) and gave 187 polymorphic markers among the Alexandrium and the Pseudo-nitzschia species. ISSR amplifications also indicated a large occurrence of simple sequence repeat ( SSR) in phytoplankton genomes,...

Tipo: Text

Palavras-chave: Tetraselmis; Skeletonema; Pseudo nitzschia; ISSR; Genetic characterization and diversity; Fingerprints; Alexandrium.

Ano: 2004

URL: http://archimer.ifremer.fr/doc/2004/publication-516.pdf

Assessment of somaclonal variation in indirect morphogenesis-derived plants of Arracacia xanthorrhiza

PAB

Vitamvas,Jan; Viehmannova,Iva; Cepkova,Petra Hlasna; Mrhalova,Hana; Eliasova,Katerina.

Abstract: The objective of this work was to induce and detect somaclonal variation in arracacha (Arracacia xanthorrhiza) plants regenerated via indirect morphogenesis, in order to evaluate the potential of this technique to produce new genotypes for breeding purposes of this crop. Calli were induced from petiole segments on Murashige & Skoog (MS) medium supplied with 0.1 mg L-1 2,4-dichlorophenoxyacetic acid. The regeneration of plants via indirect morphogenesis was carried out on half-strength MS medium without plant growth regulators. Fifteen randomly chosen plants were subjected to flow cytometry and “inter-simple sequence repeat” (ISSR) analysis. Ploidy level remained stable in all tested regenerants (2n=4x=44), with no changes in the genome....

Predicción de rendimiento de fruto de híbridos de tomate con huellas genómicas

Palavras-chave: Arracacha; ISSR; Plant breeding; Ploidy level; Somatic embryogenesis.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102200

Phyton

Hernández-Ibáñez,L; Sahagún-Castellanos,J; Rodríguez-Pérez,JE; Peña-Ortega,MG; Rodríguez-Martínez,LM.

En muchos países, el tomate (Solanum lycopersicum L.) es la especie hortícola más importante, pero la semilla de variedades sobresalientes es cara. En el mejoramiento genético mediante hibridación, el número de cruzas simples posibles es demasiado alto con pocas líneas. Por ejemplo, con 60 líneas el número de híbridos posibles es 1770. Esto hace inaccesible (en términos de costos), y aún imposible efectuar una evaluación experimental adecuada. Situaciones como ésta generan la necesidad de disponer de métodos de predicción del rendimiento de fruto de los híbridos. Este estudio se desarrolló para evaluar un método de predicción del rendimiento de fruto de híbridos basado en huellas genómicas, teoría de los modelos mixtos y en la evaluación experimental de...

URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572014000200012

Palavras-chave: Solanum lycopersicum L; Mejor predictor lineal insesgado; Marcadores moleculares; ISSR.

Ano: 2014

Assessing Genetic Variation and Spread of Phalaris minor Resistant to ACCase Inhibiting Herbicides in Iran

Planta Daninha

GHEREKHLOO,J.; ALCÁNTARA-DE LA CRUZ,R.; OSUNA,M.D.; SOHRABI,S.; PRADO,R..

ABSTRACT: Littleseed canarygrass (Phalaris minor) is the second most serious and problematic grass weed in wheat fields in Iran, and has developed resistance to ACCase inhibiting herbicides. Inter-simple sequence repeat (ISSR) analysis was used to assess genetic variation between and within ACCase inhibitor-resistant and susceptible P. minor populations in Iran and to determine the origin of resistance and its dispersal. Sixteen P. minor populations from different regions in Iran were analysed using seven primers. Genetic relationships generated using UPGMA analysis indicated the presence of more than one genotype among the herbicide resistant populations. The results indicated that the high genetic similarity and physical proximity among the resistant P....

Genetic diversity of long-established populations of Elaeis guineensis jacquin (Arecaceae)

Palavras-chave: Dendrogram; Herbicide resistance; ISSR; Genotype; UPGMA.

Ano: 2020

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582020000100226

Rev. Bras. Frutic.

Chagas,Kyvia Pontes Teixeira das; Freire,Ageu da Silva Monteiro; Pinheiro,Luciana Gomes; Fajardo,Cristiane Gouvêa; Vieira,Fábio de Almeida.

Abstract This study aimed to estimate the genetic diversity of three Elaeis guineensis populations in Rio Grande do Norte state, as well as to verify the decreases in effective population size. The population with the highest polymorphism was MAT (Mata) with+ 57 loci (72%), followed by RIA (Riacho) with 54 loci (68%) and HOR (Horta) with 34 loci (43.03%). The RIA population was shown to be the most genetically diverse, with Nei (h = 0.28) and Shannon (I = 0.41) diversity indices. There was high genetic differentiation among populations (AMOVA, analysis of molecular variance = 42%), which was separated into three distinct genetic groups according to a Bayesian analysis. There was a significant population decrease (P < 0.05) for the HOR population in the...

VARIABILIDADE GENÉTICA DE ISOLADOS DE Fusarium solani e Fusarium oxysporum f. sp.passiflorae ASSOCIADOS AO MARACUJAZEIRO

Palavras-chave: Molecular Marker; Bottleneck; ISSR; Conservation.

Ano: 2019

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000400801

Rev. Bras. Frutic.

DARIVA,JEFERSON MATEUS; XAVIER,ADELICA APARECIDA; COSTA,MARCIA REGINA; RIBEIRO,REGINA CÁSSIA FERREIRA; SOUSA,TIAGO VIEIRA.

Determinou-se a variabilidade genética de 37 isolados de Fusarium solani e 13 isolados de Fusarium oxysporum f. sp. passiflorae patogênicos ao maracujazeiro por meio do uso de marcadores ISSR e RAPD. Os isolados foram obtidos de maracujazeiros com sintomas de murcha, encontrados nas principais regiões produtoras do Norte Mineiro e no município de Sebastião Laranjeira – BA. Após obtenção de culturas monospóricas, os isolados tiveram seus DNAs extraídos e submetidos à reação de PCR. Foram selecionados nove primers ISSR e nove primersRAPD, e a análise de dados foi realizada, utilizando-se do coeficiente de similaridade de Jaccard. A partir da amplificação com os primers ISSR e RAPD, foram obtidos 121 e 126 locos, respectivamente, sendo quetodos apresentaram...

MOLECULAR CHARACTERIZATION OF JABOTICABA TREE GENOTYPES LOCATED IN THE MUNICIPALITIES OF RECÔNCAVO OF BAHIA

Palavras-chave: ISSR; RAPD; Passiflorae edulis Sims f. flavicarpaDeg.; Doenças de raiz.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452015000200377

Rev. Bras. Frutic.

CRUZ,ELAINE SILVA DA; DANTAS,ANA CRISTINA VELLO LOYOLA; CARMO,CÁTIA DIAS DO; BASTOS,LUCIMÁRIO PEREIRA.

ABSTRACT The jaboticaba tree (Plinia sp.) is a native fruit tree belonging to the Myrtaceae family, which occurs spontaneously throughout Brazil. The estimation of genetic divergence among genotypes of native populations can be useful for conservation and knowledge of available genetic resources, in the formation of gene banks and breeding species. In order to evaluate the genetic diversity of jaboticaba trees identified in the Bahia Reconcavo municipalities, 35 genotypes were characterized by ISSR primers (Inter Simple Sequence Repeats). The 18 primers generated a total of 463 amplicons, and the number of initiator fragments ranged from 14 to 36, with an average of 25.72% and 99.65% polymorphism. Polymorphic information content ranged from 0.13 to 0.33...

Molecular characterisation of radish cultivars

Palavras-chave: Genetic diversity; ISSR; Plinia sp.

Ano: 2016

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452016000300803

Rev. Ciênc. Agron.

Cruz,Sara Michelly; Nery,Marcela Carlota; Von Pinho,Édila Vilela de Resende; Laia,Marcelo Luiz de.

The radish has been gaining importance in the Brazilian market due to its use as a green manure and cover crop, and also due to its potential for the production of biodiesel, however there are only two registered cultivars, which are morphologically very similar. Determination of genetic purity is a prerequisite in marketing seeds as it ensures uniformity of the crop and is important in breeding programs. The aim of this work therefore was to evaluate the similarity and genotype patterns which permit differentiation of the commercial radish cultivars IPR 116 and CATI AL-1000. In order to do this, isoenzyme electrophoretic patterns were analysed in dry seeds, soaked seeds, seedling leaves and young leaves; the isoenzymes used being: superoxide dismutase,...

Genetic diversity of populations of the dioecious Myrsine coriacea (Primulaceae) in the Atlantic Forest

Palavras-chave: Raphanus sativus; ISSR; RAPD; Isoenzymes; Stage of development.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000400020

Acta Botanica

Paschoa,Roberta Pena da; Christ,Jheniffer Abeldt; Valente,Cecília Silva; Ferreira,Marcia Flores da Silva; Miranda,Fábio Demolinari de; Garbin,Mário Luís; Carrijo,Tatiana Tavares.

ABSTRACT Although a species’ sexual system may influence the genetic diversity of its populations in their natural environment, there have been few such studies involving indigenous species of the Atlantic Forest. Here we study Myrsine coriacea, a dioecious tree widely used in reforestation programs despite a lack of information about its natural interpopulation genetic variation. To address this knowledge gap, intra- and interpopulation genetic diversity were measured for male and female individuals of ten natural populations using ISSR markers. Greater intrapopulation genetic diversity indicated interpopulation gene flow, regardless of isolation and distance between populations. Multivariate analyses detected significant differences in genetic diversity...

Separation of the genera in the subtribe Cassiinae (Leguminosae: Caesalpinioidae) using molecular markers

Palavras-chave: Capororoca; Conservation; ISSR; Myrsinaceae; Rapanea.

Ano: 2018

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000300376

Acta Botanica

Acharya,Laxmikanta; Mukherjee,Arup Kumar; Panda,Pratap Chandra.

Random amplified polymorphic DNA (RAPD), Inter simple sequence repeat (ISSR) and Amplified fragment length polymorphism (AFLP) markers were used to verify the segregation of the genus Cassia L. senso lato into three distinct genera namely Chamaecrista Moench., Senna P. Mill. and Cassia L. sensostricto Eighteen representatives of the three taxa were characterized using the molecular markers. 25 RAPD, six ISSR primers and six AFLP primer combinations resulted in the amplification of 612, 115 and 622 bands (loci) respectively. Most of the loci are found to be polymorphic, showing high degrees of genetic diversity among the different taxa studied. The dendrogram constructed on the basis of the RAPD, ISSR and AFLP data using SHAN clustering, divided Cassia L....