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Amplitude no fósforo microbiano em um Argissolo em pastagem nativa submetida à roçada e à introdução de espécies forrageiras com fertilização fosfatada em diferentes épocas - DOI: 10.4025/actasciagron.v30i4.5319 Agronomy
Santos, Danilo Rheinheimer; UFSM; Martinazzo, Rosane; UFRGS; Gatiboni, Luciano Colpo; UDESC; Kaminski, João; UFSM; Silva, Leandro Souza da; UFSM.
Em solos com elevado grau de intemperismo, a imobilização temporária de fósforo pela biomassa microbiana do solo (BMS) pode ser um mecanismo de retardamento das reações de adsorção. O objetivo do presente trabalho foi avaliar a amplitude no fósforo microbiano (Pm) em um Argissolo em pastagem nativa submetida à roçada, introdução de forrageiras exóticas e adubadas com fertilizantes fosfatados. O estudo foi desenvolvido a partir de um experimento instalado em 1997 que constava de cinco tratamentos de fosfato e calagem (testemunha sem fósforo; hiperfosfato de gafsa; superfosfato triplo; superfosfato triplo + calcário e superfosfato simples + calcário), distribuídos em quatro blocos ao acaso. Em junho de 2002, a pastagem nativa remanescente foi roçada, semeada...
Palavras-chave: 5.01.00.00-9 Agronomia biomassa microbiana; Imobilização; Adubos fosfatados; Pastagens nativas; Disponibilidade de fósforo. microbial biomass; Immobilization; Phosphate fertilizers; Natural pasture; Phosphorus availability.
Ano: 2008 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/5319
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Production of ethanol by Saccharomyces cerevisiae and Zymomonas mobilis Coimmobilized: proposal for the use of organic waste Agrociencia
Ruiz-Marín,Alejandro; Canedo-López,Yunuén; Narváez-García,Asteria; Robles-Heredia,J. Carlos.
Abstract: Fermentation technologies were developed to improve the production of ethanol and an alternative is the immobilization technology, which offers advantages in comparison with free cells culture, such as the possibility of efficiently incorporating symbiotic bacteria in the same matrix. The aim of this study was to evaluate, in equivalent medium, the use of mango (Mangifera indica) waste to produce ethanol. The study compared the production of ethanol by using Zymomonas mobilis and Saccharomyces cerevisiae in free cells culture, immobilized and coimmobilized in calcium alginate in a culture medium equivalent to mango juice. It also included an evaluation of the effect of glucose concentration on productivity in a coimmobilized system. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mangifera indica; Immobilization; Coimmobilization; Ethanol; Zymomonas mobilis; Saccharomyces cerevisiae.
Ano: 2016 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952016000500551
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Magnetic Parkia pendula seed gum as matrix for Concanavalin A lectin immobilization and its application in affinity purification Anais da ABC (AABC)
RÊGO,MOACYR J.B.M.; ALMEIDA,SINARA M.; BEZERRA,SÉRGIO A.; CARVALHO JÚNIOR,LUIZ B.; BELTRÃO,EDUARDO I.C..
The present work aimed to magnetize Parkia pendula seeds gum and use it as a matrix for Concanavalin A covalent immobilization. This composite was applied in affinity purification of glycoconjugates. Parkia pendula seeds were hydrated and the gum provenient from the supernatant was precipitated and washed with ethanol and dried. The gum was magnetized in co-precipitation using solutions of Fe+2 and Fe+3. Matrix activation was accomplished with NaIO4. Magnetized Parkia pendula seeds gum with covalently immobilized Concanavalin A was used as an affinity matrix for the recognition of bovine serum fetuin glycoprotein. Fetuin elution was carried out with a solution of glucose (300mM) and evaluated through SDS-PAGE. The efficiency of lectin immobilization and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Magnetization; Parkia pendula seed gum; Concanavalin A.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301351
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Advances in enzyme bioelectrochemistry Anais da ABC (AABC)
PEREIRA,ANDRESSA R.; SEDENHO,GRAZIELA C.; SOUZA,JOÃO C. P. DE; CRESPILHO,FRANK N..
ABSTRACT Bioelectrochemistry can be defined as a branch of Chemical Science concerned with electron-proton transfer and transport involving biomolecules, as well as electrode reactions of redox enzymes. The bioelectrochemical reactions and system have direct impact in biotechnological development, in medical devices designing, in the behavior of DNA-protein complexes, in green-energy and bioenergy concepts, and make it possible an understanding of metabolism of all living organisms (e.g. humans) where biomolecules are integral to health and proper functioning. In the last years, many researchers have dedicated itself to study different redox enzymes by using electrochemistry, aiming to understand their mechanisms and to develop promising bioanodes and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioelectrocatalysis; Biofuel cells; Bioelectrochemistry; Immobilization; Protein-electrode interactions; Redox enzymes.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000200825
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Comparative performance of microbial lipases immobilized on magnetic polysiloxane polyvinyl alcohol particles BABT
Bruno,Laura Maria; Lima Filho,José Luiz de; Castro,Heizir Ferreira de.
Microbial lipase from Mucor miehei and Candida rugosa were immobilized by covalent binding onto magnetized polysiloxane polyvinyl alcohol particles (POS-PVA). The resulting immobilized derivatives were evaluated in aqueous solution (olive oil hydrolysis) and organic solvent (butyl butyrate synthesis). Higher catalytic activities were found when the coupling procedure was made with M. miehei lipase. Immobilized M. miehei lipase also showed a better operational stability and a higher half-life than C. rugosa lipase after the successive batches of esterification. The performance of C. rugosa immobilized derivative was constrained by the low lipase loading used in the immobilizing step. Further information regarding the both immobilized derivatives was also...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida lipase; Mucor lipase; Hybrid matrix; Immobilization.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500003
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Metabolic and morphometric alterations inherent to neuromuscular electric stimulation in the antagonist muscle submitted to ankle joint immobilization BABT
Durigan,João Luiz Quagliotti; Cancelliero,Karina Maria; Bosi,Paula Lima; Delfino,Gabriel Borges; Montebelo,Maria Imaculada de Lima; Guirro,Rinaldo Roberto de Jesus; Silva,Carlos Alberto da; Polacow,Maria Luiza Ozores.
The aim of this work was to investigate the effect of the neuromuscular electrical stimulation (ES) on the metabolic and morfometric profile of the tibialis anterior muscle, antagonist to the soleus muscle which was stimulate, under the joint immobilization condition of the ankle for 7 and 15 days. The immobilization promoted the reduction in the muscle mass (I7: 17.36%, I15: 20.83%), in the glycogen content (I7: 48%, I15: 48%), in the muscle fibers areas (I7: 27%, I15: 40%) and increase in intramuscular connective tissue density (I7: 122%, I15: 206%). The EE didn't promote significant alterations in the mass of the immobilized groups, however, promoted increase in the glycogen (IEE7: 31.25%; IEE15: 56.25%), reduction in the muscle fibers areas (IEE7: 14%,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Muscle disuse; Metabolic; Morphometric; Neuromuscular electrical stimulation; Rehabilitation.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100011
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Statistical Modelling and Optimization of Fermentation Medium for Lincomycin Production by Streptomyces lincolnensis Immobilized Cells BABT
Abdelwahed,Nayera A.M.; Gomaa,Eman Zakaria; Hassan,Amira A..
ABSTRACT Response surface methodology was used to optimize lincomycin production by Streptomyces lincolnensis NRRL ISP-5355 in submerged fermentation. Screening of fermentation medium components to find their relative effect on lincomycin production was done using Plackett-Burman design. Malt extract, dextrin, soluble starch and (NH4)2SO4 were the most significant nutrient influenced on lincomycin production. Central composite design was applied to determine optimal concentrations of these factors and the effect of their mutual interactions. The interaction between soluble starch and (NH4)2SO4 was found to enhance the production, whereas malt extract and dextrin exhibited an influence independent from the other two factors. Using this statistical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Optimization; Lincomycin; Streptomyces lincolnensis; Response surface methodology; Immobilization.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100405
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Adsorption, immobilization and activity of cellulase in soil: the impacts of maize straw and its humification BABT
Sinegani,Ali Akbar Safari; Sinegani,Mahboobe Safari.
The present work aimed to study some aspects of sorption and immobilization of cellulase molecules on soil components by the analysis of the reactions of cellulase in a soil treated with different levels of maize residue and incubated for 90 days. The analysis of variance showed that the effects of the treatments of maize straw, incubation time and their interaction on cellulase adsorption, desorption and immobilization were statistically significant. The adsorption and immobilization capacities of soil by application of maize straw increased significantly. However they decreased with decreasing the soil organic matter (SOM) after 45 days of incubation. The desorption of adsorbed cellulase molecules from the soil by washing with distilled water depended on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Maize straw; Humification; Cellulase; Sorption; Immobilization; Activity.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000600001
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Effect of raffinose and ultrasound pulses on invertase release by free and immobilized Saccharomyces cerevisiae in loofa (Luffa cylindrica) sponge BABT
Marques,Leila Larisa Medeiros; Buzato,João Batista; Celligoi,Maria Antonia Pedrine Colabone.
This study investigated the effect of raffinose and ultrasound pulses on invertase release from free S. cerevisiae and S. cerevisiae immobilized in Luffa cylindrica. The free cell culture was submitted to 2% raffinose pulse and irradiated for 2 minutes at 0.12 and 0.46 h-1 dilution rates. The immobilized cell culture was submitted to raffinose pulse and irradiated for 1, 2 and 4 minutes, at 0.10 h-1 dilution rate. In immobilized cells, the raffinose pulse increased the invertase activity from 5.38 to 7.27 U/mg. Ultrasound application in free cell culture at the 0.12 h-1 dilution rate gave the best results. The activity varied from 25.08 to 29.38 U/mg while the increase in immobilized cells was from 5.22 to 9.70 U/mg when sonicated for two minutes. These...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Invertase; Luffa cylindrica; Raffinose; Saccharomyces cerevisiae; Ultrasound.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000700003
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Hydrolysis of whey lactose by immobilized β-Galactosidase BABT
Mariotti,Marcela Panaro; Yamanaka,Hideko; Araujo,Angela Regina; Trevisan,Henrique Celso.
Hydrolysis of whey lactose to glucose and galactose by immobilized galactosidase comes as an alternative to enlarge the possibilities of commercial use of this feedstock. To be applied at industrial scale, the process should be performed continuously .This work aimed to study the hydrolysis of whey lactose by an immobilized enzyme reactor. b-Galactosidase from Aspergillus oryzae was immobilized on silica and activity and stability were evaluated. The best immobilization results were attained by using glutaraldehyde as support's activator and enzyme stabilizer. The optimized enzyme proportion for immobilization was 15-20 mg g-1 of support. Treatments of whey were performed (microfiltration, thermal treatment and ultrafiltration), seeking the elimination of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Β-galactosidase; Immobilization; Lactase; Whey; Lactose; Hydrolysis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600019
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Immobilization of Beauveria bassiana Lipase on Silica Gel by Physical Adsorption BABT
Sugahara,Vanessa Hitomi; Varéa,Geni da Silva.
Extracellular lipase from Beauveria bassianastrain CG481 was immobilized by using thirteen different immobilization protocols. Silica gel was chosen as the most suitable adsorbent with 94.8% of activity yield. The adsorption on silica gel did not change the optimum pH (8.5) and temperature (45ºC) values of the free lipase (FL) for lipolytic activity, and it showed higher activities in extreme conditions (pH 9.0 to 10.5, 60ºC). The lipase immobilized on silica gel (ILS) showed enhanced stability at pH 7.0 after 120 h incubation (69.0%) when compared to FL (33.3%). The thermal stability was also enhanced by immobilization at 60ºC in aqueous (64.6%) and organic medium (95.1%), while FL showed only 40.6% of residual activity in aqueous medium and exhibited no...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Lipase; Beauveria bassiana; Silica gel.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600842
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A comparative study on fungal laccases immobilized on chitosan BABT
Araújo,José Hilton Bernardino de; Uemura,Vinicius Oliveira; Moraes,Flavio Faria de; Barbosa,Aneli de Melo; Zanin,Gisella Maria.
The phenoloxidase enzyme laccase from the cultures of the Pleurotus ostreatus and Botryosphaeria sp. and a commercial laccase from Aspergillus sp. were immobilized on chitosan of pharmaceutical degree by adsorption followed by crosslinking. Different immobilization conditions in relation to the granulometry of support and amount of enzymatic laccase extract used were tested, aiming at reaching high enzymatic activity with the immobilized enzyme. Two different substrates, ABTS and DMP, were used for the determination of enzymatic activity. The highest enzymatic activity was obtained when 1.0mg/mL of the enzymatic laccase extract from Botryosphaeria sp. was used with 1.0g of support (200 mesh). These immobilized enzymes are to be applied to the improvement...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Laccases; Immobilization; Chitosan.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000400001
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Use of Coffee Capsules as Support Material in Upflow Anaerobic Fixed Bed Reactors BABT
Souza,Gabriela Rezende de; Oliveira,Luiz Fernando Coutinho de; Bello,Italoema Pinheiro; Siniscalchi,Luciene Alves Batista; Fia,Ronaldo; Gandia,Rômulo Marçal.
Abstract The aim of this work is to evaluate the performance of upflow anaerobic fixed bed reactors filled with espresso coffee capsules to treat sanitary sewage. Three reactors (R1, R2 and R3) were constructed in blue PVC pipes measuring 30 cm height and 150 mm diameter and filled with coffee capsules made of aluminum and plastic. The sewage from the pre-treatment phase of the wastewater treatment plant of the Federal University of Lavras fed the system. Temperature, pH, alkalinity and volatile acids concentration, COD, TS, TVS and TSS of the influent and effluent were analyzed to evaluate the reactors performances. Statistics tests were run in the software Statistica 10. Changes occurred in the organic loading rates caused two different operating phases,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anaerobic filter; Biomass; Immobilization; Support material; Domestic sewage.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100223
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Role of Glutaraldehyde in Imparting Stability to Immobilized β-Galactosidase Systems BABT
Satar,Rukhsana; Jafri,Mohammad Alam; Rasool,Mahmood; Ansari,Shakeel Ahmed.
ABSTRACT This review article highlights the role of glutaraldehyde as a matrix activator/stabilizer in imparting higher operational and thermal stability to β-galactosidase (βG) for biotechnological applications. Glutaraldehyde has been used extensively as a crosslinking agent as well as for functionalization of matrices to immobilize β-galactosidase. Immobilized β-galactosidase systems (IβGS) obtained as a result of glutaraldehyde treatment has been employed to hydrolyze whey and milk lactose in batch reactors, continuous packed-bed and fluidized bed reactors under various operational conditions. Moreover, these IβGS have also been utilized for the production of galactooligosaccharides in food, dairy and fermentation industries. It was observed that...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Β-galactosidase; Crosslinking; Glutaraldehyde; Immobilization; Stability.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100447
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Immobilization of the Magnetic Nanoparticles with Alkaline Protease Enzyme Produced by Enterococcus hirae and Pseudomonas aeruginosa Isolated from Dairy Effluents BABT
Masi,Chandran; Chandramohan,C.; Ahmed,M.Fazil.
ABSTRACT Protease is an enzyme which has a wide range of applications in various fields. Extracellular protease was produced from Pseudomonas aeruginosa and Enterococcus hirae which were isolated from the effluents of diary industries. Protease immobilized with super paramagnetic nanoparticles was characterized by DLS, XRD and TEM methods in relation to their size and structure. The protease enzyme was bound to magnetic nanoparticles via surface transformation technique including Silica coated magnetic Nano composite, amine and cysteine functioned Nano composite formation. Successful binding of protease onto the particles was confirmed by TEM imaging. The maximal enzyme activity of immobilized protease was determined using universal protease assay and was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Protease; Super paramagnetic Nanoparticle; Nano composite; Immobilization; Activity and Stability.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100462
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Characteristics of Immobilized Urease on Grafted Alginate Bead Systems BABT
Danial,Enas N.; Hamza,Amal H.; Mahmoud,Rasha H..
This study evaluated the biological importance of immobilized urease enzyme over the free urease. The support material used for urease immobilization was alginate. Generally, the immobilization of urease in alginate gel showed a marked increase in Km and Vmax. However, the immobilized urease showed higher thermal stability than that of free enzyme. The rate of thermal inactivation of the immobilized enzyme decreased due to entrapment in gel matrix. Also, the activity of the immobilized urease was more stable in retention than that of the free enzyme during the storage in solution, although the activity of the immobilized enzyme was lower in comparison with the free enzyme. A stable immobilized system and long storage life are convenient for applications...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Urease; Immobilization; Kinetic; Reusability; Storage stability.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200147
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The production of sorbitol by permeabilized and immobilized cells of Z. mobilis in sucrose BABT
Vignoli,Josiane Alessandra; Celligoi,Maria Antonia Colabone; Silva,Rui Sérgio Ferreira da; Barros,Márcio de.
The production of sorbitol by permeabilized and immobilized cells of Zymomonas mobilis in Luffa cylindrica was investigated in sucrose medium. A full 2³ factorial design was used to verify the influence of each factor and its interactions. The cell permeabilization showed a significant and negative effect upon the production of sorbitol, while the time of cultivation and the immobilization process were significant and positive. The results demonstrated that the cell immobilization and the time of cultivation of 36 h presented higher production of sorbitol.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Permeabilization; Immobilization; Sucrose; Z. mobilis.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500020
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Three days of intermittent stretching after muscle disuse alters the proteins involved in force transmission in muscle fibers in weanling rats BJMBR
Gianelo,M.C.S.; Polizzelo,J.C.; Chesca,D.; Mattiello-Sverzut,A.C..
The aim of this study was to determine the effects of intermittent passive manual stretching on various proteins involved in force transmission in skeletal muscle. Female Wistar weanling rats were randomly assigned to 5 groups: 2 control groups containing 21- and 30-day-old rats that received neither immobilization nor stretching, and 3 test groups that received 1) passive stretching over 3 days, 2) immobilization for 7 days and then passive stretching over 3 days, or 3) immobilization for 7 days. Maximal plantar flexion in the right hind limb was imposed, and the stretching protocol of 10 repetitions of 30 s stretches was applied. The soleus muscles were harvested and processed for HE and picrosirius staining; immunohistochemical analysis of collagen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Immobilization; Vimentin; Desmin; Intermittent stretching; Collagen.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000200603
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Analysis of fracture healing in osteopenic bone caused by disuse: experimental study BJMBR
Paiva,A.G.; Yanagihara,G.R.; Macedo,A.P.; Ramos,J.; Issa,J.P.M.; Shimano,A.C..
Osteoporosis has become a serious global public health issue. Hence, osteoporotic fracture healing has been investigated in several previous studies because there is still controversy over the effect osteoporosis has on the healing process. The current study aimed to analyze two different periods of bone healing in normal and osteopenic rats. Sixty, 7-week-old female Wistar rats were randomly divided into four groups: unrestricted and immobilized for 2 weeks after osteotomy (OU2), suspended and immobilized for 2 weeks after osteotomy (OS2), unrestricted and immobilized for 6 weeks after osteotomy (OU6), and suspended and immobilized for 6 weeks after osteotomy (OS6). Osteotomy was performed in the middle third of the right tibia 21 days after tail...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Osteoporosis; Osteotomy; Fracture healing; Immobilization; Rats.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000300708
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Effect of one stretch a week applied to the immobilized soleus muscle on rat muscle fiber morphology BJMBR
Gomes,A.R.S.; Coutinho,E.L.; França,C.N.; Polonio,J.; Salvini,T.F..
We determined the effect of stretching applied once a week to the soleus muscle immobilized in the shortened position on muscle fiber morphology. Twenty-six male Wistar rats weighing 269 ± 26 g were divided into three groups. Group I, the left soleus was immobilized in the shortened position for 3 weeks; group II, the soleus was immobilized in the shortened position and stretched once a week for 3 weeks; group III, the soleus was submitted only to stretching once a week for 3 weeks. The medial part of the soleus muscle was frozen for histology and muscle fiber area evaluation and the lateral part was used for the determination of number and length of serial sarcomeres. Soleus muscle submitted only to immobilization showed a reduction in weight (44 ± 6%, P...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Muscle; Stretching; Immobilization; Sarcomere number; Muscle fiber area.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004001000005
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