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	Provedor de dados BJMBR (3) Biol. Res. (1) Autor Brink,P.R. (2) Banach,K. (1) Beyer,E.C. (1) Christ,G.J. (1) Court,Felipe A (1) Gemel,J. (1) Larson,D.M. (1) Lopez-Verrilli,María A (1) Rozental,R. (1) Seul,K.H. (1) Spray,D.C (1) Urban,M. (1) Mais... Palavra-chave Intercellular communication (4) Gap junctions (2) CDNA sequences (1) Connexin37 (1) Connexin40 (1) Connexin43 (1) Endothelium (1) Exosome (1) Heteromeric connexons (1) Multi primers (1) Nanovesicle (1) Rat aorta (1) Universal primers (1) Vascular smooth muscle (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Journal article (1) Ano 2013 (1) 2000 (2) 1999 (1) País Brazil (3) Chile (1) Idioma Inglês (4)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 4 Primeira ... 1 ... Última Exosomes: mediators of communication in eukaryotes Biol. Res. Lopez-Verrilli,María A; Court,Felipe A. In addition to the established mechanisms of intercellular signaling, a new way of communication has gained much attention in the last decade: communication mediated by exosomes. Exosomes are nanovesicles (with a diameter of 40-120 nm) secreted into the extracellular space by the multivesicular endosome after its outer membrane fuses with the plasma membrane. Once released, exosomes modulate the response of the recipient cells that recognize them. This indicates that exosomes operate in a specific manner and participate in the regulation of the target cell. Remarkably, exosomes occur from unicellular organisms to mammals, suggesting an evolutionarily conserved mechanism of communication. In this review we describe the cascade of exosome formation,... Tipo: Journal article Palavras-chave: Exosome; Nanovesicle; Intercellular communication. Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100001 Modulation of intercellular communication by differential regulation and heteromeric mixing of co-expressed connexins BJMBR Beyer,E.C.; Gemel,J.; Seul,K.H.; Larson,D.M.; Banach,K.; Brink,P.R.. Intercellular communication may be regulated by the differential expression of subunit gap junction proteins (connexins) which form channels with differing gating and permeability properties. Endothelial cells express three different connexins (connexin37, connexin40, and connexin43) in vivo. To study the differential regulation of expression and synthesis of connexin37 and connexin43, we used cultured bovine aortic endothelial cells which contain these two connexins in vitro. RNA blots demonstrated discordant expression of these two connexins during growth to confluency. RNA blots and immunoblots showed that levels of these connexins were modulated by treatment of cultures with transforming growth factor-ß1. To examine the potential ability of these... Tipo: Info:eu-repo/semantics/article Palavras-chave: Endothelium; Heteromeric connexons; Intercellular communication; Connexin37; Connexin40. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400004 Gap junctions in isolated rat aorta: evidence for contractile responses that exhibit a differential dependence on intercellular communication BJMBR Christ,G.J.; Brink,P.R.. Connexin43 (Cx43) is a major gap junction protein present in the Fischer-344 rat aorta. Previous studies have identified conditions under which selective disruption of intercellular communication with heptanol caused a significant, readily reversible and time-dependent diminution in the magnitude of <FONT FACE="Symbol">a</font>1-adrenergic contractions in isolated rat aorta. These observations have indentified a significant role for gap junctions in modulating vascular smooth muscle tone. The goal of these steady-state studies was to utilize isolated rat aortic rings to further evaluate the contribution of intercellular junctions to contractions elicited by cellular activation in response to several other vascular spasmogens. The effects of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Gap junctions; Connexin43; Rat aorta; Vascular smooth muscle; Intercellular communication. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000400008 A simple RT-PCR-based strategy for screening connexin identity BJMBR Urban,M.; Rozental,R.; Spray,D.C. Vertebrate gap junctions are aggregates of transmembrane channels which are composed of connexin (Cx) proteins encoded by at least fourteen distinct genes in mammals. Since the same Cx type can be expressed in different tissues and more than one Cx type can be expressed by the same cell, the thorough identification of which connexin is in which cell type and how connexin expression changes after experimental manipulation has become quite laborious. Here we describe an efficient, rapid and simple method by which connexin type(s) can be identified in mammalian tissue and cultured cells using endonuclease cleavage of RT-PCR products generated from "multi primers" (sense primer, degenerate oligonucleotide corresponding to a region of the first extracellular... Tipo: Info:eu-repo/semantics/article Palavras-chave: Gap junctions; Intercellular communication; CDNA sequences; Universal primers; Multi primers. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000800014 Registros recuperados: 4 Primeira ... 1 ... Última

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