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| Gomes,Eliane A.; Kasuya,Maria Catarina M.; Barros,Everaldo G. de; Borges,Arnaldo C.; Araújo,Elza F.. |
| Inter- and intraspecific variation among 26 isolates of ectomycorrhizal fungi belonging to 8 genera and 19 species were evaluated by analysis of the internal transcribed sequence (ITS) of the rDNA region using restriction fragment length polymorphism (RFLP). The ITS region was first amplified by polymerase chain reaction (PCR) with specific primers and then cleaved with different restriction enzymes. Amplification products, which ranged between 560 and 750 base pairs (bp), were obtained for all the isolates analyzed. The degree of polymorphism observed did not allow proper identification of most of the isolates. Cleavage of amplified fragments with the restriction enzymes Alu I, Hae III, Hinf I, and Hpa II revealed extensive polymorphism. All eight genera... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: DNA fingerprinting; Ectomycorrhizal fungi; Internal transcribed spacer (ITS); PCR-RFLP; Ribosomal DNA (rDNA). |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400018 |
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| Muellner, A.N.; Mabberley, D.J.. |
| For decades, the rare Malesian shrublet hitherto called Turraea breviflora, was the only species of the pantropical family Meliaceae which could not confidently be placed in a genus. Previous morphological re-investigation led to the exclusion of the species from Turraea and suggested possibly close links with Munronia. In this study, parsimony, maximum likelihood and Bayesian analyses of DNA sequence data from plastid rbcL and nuclear ITS rDNA were used to infer the phylogenetic position of T. breviflora and its affinities to other representatives of the family. Phylogenetic analyses support Mabberley’s (1995a) proposal that the concept of the genus Munronia be expanded to accommodate it. The new combination Munronia breviflora (Ridl.) Mabb. &... |
| Tipo: Article / Letter to the editor |
Palavras-chave: Meliaceae; Munronia; Sapindales; Turraea breviflora; Internal transcribed spacer (ITS); RbcL; Taxonomy. |
| Ano: 2008 |
URL: http://www.repository.naturalis.nl/record/525100 |
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| Kasuya,Maria Catarina Megumi; Coelho,Irene da Silva; Campos,Daniela Tiago da Silva; Araújo,Elza Fernandes de; Tamai,Yutaka; Miyamoto,Toshizumi. |
| Eighteen Pisolithus basidiomes were collected from Eucalyptus plantations in the state of Minas Gerais, Brazil. These basidiomes were characterized morphologically and molecularly. The basidiomes varied in shape, color and size. One of them was found underground, indicating a hypogeous fungus. The main morphological distinctive characteristic was spore ornamentation, which distinguished two groups. One group with short and erect spines was identified as Pisolithus microcarpus, and the other with long and curved spines as Pisolithus marmoratus, after analyzing the cladogram obtained by phylogenetic relationship based on internal transcribed spacer (ITS) regions of the nuclear ribosomal DNA of these isolates. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ectomycorrhizal fungus; Phylogenetic analysis; Internal transcribed spacer (ITS); Nuclear ribosomal DNA; Taxonomy. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832010000600013 |
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