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| Silva,Edson Cabral da; Muraoka,Takashi; Franzini,Vinícius Ide; Villanueva,Felipe Carlos Alvarez; Buzetti,Salatiér; Moreti,Dolorice. |
| The objective of this work was to evaluate the utilization by corn plants of P from triple superphosphate fertilizer labeled with 32P (32P‑TSP), and of P from soil as affected by N rates and by the green manures (GM) sunn hemp (Crotalaria juncea) and millet (Pennisetum glaucum). The experiment was carried out using pots filled with 5 kg Oxisol (Rhodic Hapludox). A completely randomized design was used, in a 4x4x2 factorial arrangement, with four replicates. The treatments were: four P rates as TSP (0, 0.175, 0.350, and 0.700 g P per pot); four N rates as urea (0, 0.75, 1.50, and 2.25 g N per pot); and sunn hemp or millet as green manure. The additions of N and P by the GM were taken into account. After grain physiologic maturation, corn dry matter, P... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zea mays; Cover crop; Isotopic dilution; Oxisol; Radioisotope; Urea. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000800016 |
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| Antunes,Dalton Araujo; Louvandini,Helder; Silva Filho,José Cleto da; McManus,Concepta Margaret; Dallago,Bruno Steffano; Machado,Bruno de Oliveira; Mendonça,Dharana Guedes; Correa,Patrícia Spoto. |
| Este trabalho teve como objetivo avaliar a técnica de incorporação de 32P pelos eritrócitos de ovinos jovens, mantidos em pastejo de Andropogon gayanus, como método de diagnóstico da deficiência de fósforo. Vinte ovinos, com peso vivo inicial de 13,88±2,51 kg, foram divididos em dois tratamentos de dez animais cada; num dos tratamentos, os animais foram suplementados com 3 g de P por animal por dia e, no outro, os animais não receberam suplementação de P. Foram realizadas cinco pesagens dos animais, coletas de sangue e fezes nos 8º, 29º, 43º, 57º e 71º dias do experimento, para avaliar a incorporação de 32P pelos eritrócitos, determinar as concentrações de Ca, glicose e P no soro e a porcentagem de P nas fezes. Foi encontrada diferença significativa na... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Andropogon gayanus; Nutrição animal; Diluição isotópica; Santa Inês; Hemácias; Andropogon gayanus; Animal nutrition; Isotopic dilution; Hair sheep; Haemacytes. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000200020 |
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| Stradiotti,AC; Bendassolli,JA; Ducatti,C; Sartori,JR; Pelícia,VC; Araujo,PC; Maruno,MK; Pezzato,AC. |
| ABSTRACT The objective of this study was to evaluate the process of L-methionine incorporation in the blood plasma, liver, breast muscle, and abdominal fat of 35- to 59-d-old broiler chickens using the carbon stable isotope (12C and 13C) technique for the estimation of methionine requirements. In this experiment, 51 male broiler chickens orally received a solution of L-[13C1] methionine (92 atm % 13C) at 29 µmol/kg live weight/h for 6 h. Three birds were sacrificed for tissue collection at times 0 h (control), 0.5, 1, 2, 3, 4, 5, 6, 12, 24, 48, 72, 96, 120, 144, 168, and 336 h after the administration of the first dose. Tissue L-[13C1] methionine incorporation mass and percentage results were analyzed using Minitab 16 statistical software. Except for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Isotopic dilution; Labeled isotopes; Turnover. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000400719 |
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| Rossete,Alexssandra Luiza Rodrigues Molina; Carneiro,Josiane Meire Toloti; Sant Ana Filho,Carlos Roberto; Bendassolli,José Albertino. |
| Sulphur plays an essential role in plants and is one of the main nutrients in several metabolic processes. It has four stable isotopes (32S, 33S, 34S, and 36S) with a natural abundance of 95.00, 0.76, 4.22, and 0.014 in atom %, respectively. A method for isotopic determination of S by isotope-ratio mass spectrometry (IRMS) in soil samples is proposed. The procedure involves the oxidation of organic S to sulphate (S-SO4(2-)), which was determined by dry combustion with alkaline oxidizing agents. The total S-SO4(2-) concentration was determined by turbidimetry and the results showed that the conversion process was adequate. To produce gaseous SO2 gas, BaSO4 was thermally decomposed in a vacuum system at 900 ºC in the presence of NaPO3. The isotope... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sample preparation; Soil samples; Isotopic dilution; Labeled material; Stable isotope; 34S. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832012000600012 |
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| SALEH,MAYRA A.D.; SANTOS,LUAN S. DOS; BERTO,DIRLEI A.; AMORIM,ALESSANDRO B.; COSTA,VLADIMIR E.; DUCATTI,CARLOS. |
| Abstract Isotope-ratio mass spectrometry (IRMS) is a potential tool that provides time-integrated estimate of assimilated and not just ingested nutrients. This feature turned possible its application to evaluate the effects of dietary nucleotides and glutamate on carbon turnover (δ13C) in the brain of weanling piglets. Eighty-seven piglets weaned at 21-day-old were used, being three piglets slaughtered at day-0 (prior to diet-switch) and, the remaining 84 randomly assigned in a complete block design with a 2 x 2 factorial arrangement of treatments (two nucleotide levels: 0% and 0.1% and, two glutamate levels: 0% and 1%). On trial days 3, 6, 9, 14, 21, 35 and 49, three piglets per treatment were also slaughtered. The samples were analyzed by IRMS and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Additives; Amino acids; Isotopic dilution; Stable isotopes; Swine. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000502469 |
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