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| Seki,Liliane Miyuki; Pereira,Polyana Silva; Conceição,Magda de Souza; Souza,Maria José; Marques,Elizabeth Andrade; Carballido,Jupira Miron; Carvalho,Maria Elisabeth Serqueira de; Assef,Ana Paula D'Alincourt Carvalho; Asensi,Marise Dutra. |
| OBJECTIVE: The present studywas designed to evaluate the molecular epidemiology of CTX-M producing Klebsiella pneumoniae, Enterobacter cloacae and Escherichia coli isolated from bloodstream infections at tertiary care hospitals in the State of Rio de Janeiro, Brazil. MATERIAL AND METHODS: A total of 231 nonduplicate Enterobacteriaceae were isolated from five Brazilian hospitals between September 2007 and September 2008. The antimicrobial susceptibility testing was performed by disk diffusion method according to the Clinical Laboratory Standard Institute. Isolates showing resistance to third-generation cephalosporins were screened for ESBL activity by the double-disk synergy test. The presence of blaCTX-M , blaCTX-M-15 and blaKPC genes was determined by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bloodstream infections; Enterobacteriaceae; CTX-M-15; PFGE; KPC; Brazil. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000600003 |
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| Sağıroğlu,Pınar; Hasdemir,Ufuk; Gelmez,Gülşen Altınkanat; Aksu,Burak; Karatuna,Onur; Söyletir,Güner. |
| ABSTRACT In this study, the performance of the “RESIST-3 O.K.N. K-SeT” (Coris BioConcept, Gembloux, Belgium) immunochromatographic assay was evaluated in 132 Klebsiella pneumoniae comprising 102 carbapenem resistant and 30 carbapenem susceptible isolates. Genotypically known isolates of Gram negative bacteria (n = 22) including various species were also tested by the assay as controls. The isolates tested by the immunochromatographic assay and also were run PCR for bla KPC, bla IMP, bla VIM, bla NDM, and bla OXA-48. The rates of bla NDM, bla OXA-48, and bla KPC in carbapenem resistant isolates were found at 52.9%, 39.2%, and 2.0%, respectively. Both bla NDM and bla OXA-48 were found in six (5.9%) isolates. The results of the assay showed 100% concordance... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Klebsiella pneumoniae; Immunochromatographic test; NDM; KPC; OXA-48. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400885 |
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| Rechenchoski,Daniele Zendrini; Dambrozio,Angélica Marim Lopes; Vivan,Ana Carolina Polano; Schuroff,Paulo Alfonso; Burgos,Tatiane das Neves; Pelisson,Marsileni; Perugini,Marcia Regina Eches; Vespero,Eliana Carolina. |
| Abstract The production of KPC (Klebsiella pneumoniae carbapenemase) is the major mechanism of resistance to carbapenem agents in enterobacterias. In this context, forty KPC-producing Enterobacter spp. clinical isolates were studied. It was evaluated the activity of antimicrobial agents: polymyxin B, tigecycline, ertapenem, imipenem and meropenem, and was performed a comparison of the methodologies used to determine the susceptibility: broth microdilution, Etest® (bioMérieux), Vitek 2® automated system (bioMérieux) and disc diffusion. It was calculated the minimum inhibitory concentration (MIC) for each antimicrobial and polymyxin B showed the lowest concentrations for broth microdilution. Errors also were calculated among the techniques, tigecycline and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antimicrobial activity; Enterobacter spp.; KPC; Methods of susceptibility. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300509 |
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| Ramos,Gustavo Luis de Paiva Anciens; Nascimento,Janaína dos Santos. |
| ABSTRACT: Goat’s milk has been suggested as an alternative to cow’s milk, being a better digestible and hypoallergenic option. However, the presence of contaminating bacteria may significantly affect the safety of the product. In this research, we reported the isolation and characterization of Acinetobacter spp. isolates from raw goat milk samples purchased in the state of Rio de Janeiro, Brazil. Twenty-one samples were analyzed and ten isolates of Acinetobacter spp. were obtained. Six were identified as A. guillouiae, three as A. ursingii, and one as A. bereziniae. These isolates were characterized and eight showed proteolytic activity, seven showed lipolytic activity, and five isolates were able to produce both enzymes. None of the isolates was biofilm... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Goat milk; Acinetobacter; Deteriorating enzymes; KPC; ESBL. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001000451 |
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