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Hess, Philipp. |
An overview is given of the biological origin of phycotoxins, as well as their chemical characteristics. Major poisoning types are described and examples of poisoning events are given to illustrate the importance of the phenomenon to both shellfish consumers and the shellfish producing industry. The characteristics of phycotoxins as natural products, the lack of predictability of their occurrence, economic drivers and the freshness of shellfish consumed in many countries result in a number of requirements for methods to be used in the efficient detection of these compounds. Subsequently, the performance of mouse bioassays and mass spectrometry as detection tools are compared for examples from Irish and French monitoring programmes to assess the usefulness... |
Tipo: Text |
Palavras-chave: Bioanalytical methods; Bioassays; Mass spectrometry; Liquid chromatography; Biological samples. |
Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00006/11767/8989.pdf |
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Tang,MS; Vejayan,J; Ibrahim,H. |
Two-dimensional gel electrophoresis (2DE) is an important tool for investigating the complexity of snake venom proteomes. Apart from applications based on whole proteome analysis, we suggest that 2DE can be used as an assay to guide the progress of protein purification. The aim of this study was to prove the feasibility of this concept by using it to purify rhodocetin from Calloselasma rhodostoma venom. Rhodocetin (α subunit) spot on the 2DE profile of C. rhodostoma venom was first identified and confirmed by mass spectrometry, with a molecular mass of 16 kDa and calculated pI of 5.16. Rhodocetin was subsequently purified by successive anion-exchange and gel filtration chromatography. Every peak from both chromatography profiles was collected and tested on... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Protein fingerprinting; Liquid chromatography; Proteomics; Venom. |
Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400011 |
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SILVA,K.S.; FREITAS,F.C.L.; BRAGA,D.F.; DOMBROSKI,J.L.D.; SANTOS,A.F.B.. |
ABSTRACT Ametryn is one of the most widely used herbicides in the sugarcane culture. Little is known about the interactions between this herbicide and the attributes of soils in the sugarcane region of northeastern Brazil. This knowledge, before recommending herbicide, will minimize the negative effects on the environment, particularly on water resources, and will ensure weed control efficacy. In this work, ametryn leaching potential was estimated through bioassays and chromatography, in five soils from the sugarcane region in northeastern Brazil: Quartzarenic Neosol (Entisol); Red Argisol (Ultisol); Ferrihumiluvic Spodosol (Spodosols); Red-Yellow Acrisol (Oxisol) and Haplic Cambisol (Inceptisols). To achieve this, columns were prepared with samples of the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Herbicide; Liquid chromatography; Bioassay; Mobility. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000400839 |
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