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| Registros recuperados: 25 | |
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| Pérez-Morales,Rebeca; Méndez-Ramírez,Ignacio; Castro-Hernández,Clementina; Martínez-Ramírez,Ollin C.; Gonsebatt,María Eugenia; Rubio,Julieta. |
| Lung cancer is the leading cause of cancer mortality in Mexico and worldwide. In the past decade, there has been an increase in the number of lung cancer cases in young people, which suggests an important role for genetic background in the etiology of this disease. In this study, we genetically characterized 16 polymorphisms in 12 low penetrance genes (AhR, CYP1A1, CYP2E1, EPHX1, GSTM1, GSTT1, GSTPI, XRCC1, ERCC2, MGMT, CCND1 and TP53) in 382 healthy Mexican Mestizos as the first step in elucidating the genetic structure of this population and identifying high risk individuals. All of the genotypes analyzed were in Hardy-Weinberg equilibrium, but different degrees of linkage were observed for polymorphisms in the CYP1A1 and EPHX1 genes. The genetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Additive model; Genetic polymorphism; Lung cancer; Mexicans; Molecular epidemiology. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400003 |
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| Gorgisen,Gokhan; Hapil,Fatma Zehra; Yilmaz,Ozlem; Cetin,Zafer; Pehlivanoglu,Suray; Ozbudak,Irem Hicran; Erdogan,Abdullah; Ozes,Osman Nidai. |
| Abstract Lung cancer is the leading cause of cancer-related death, and NSCLC constitutes nearly 85%–90% of all cases. The IRS proteins function as adaptors and transmit signals from multiple receptors. Upon binding of insulin to the insulin receptor (IR), IRS1 is phosphorylated at several YXXM motifs creating docking sites for the binding of PI3Kp85, which activates AKT kinase. Therefore, we thought that gain of function mutantions of IRS1 could be related to development of lung cancer. In line with this, we wanted determine whether the IRS1 gene was mutated in the coding regions surrounding YXXM motifs. We sequenced the coding regions surrounding YXXM motifs of IRS1 using tumor samples of 42 NSCLC patients and 40 matching controls and found heterozygote... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: IRS1; NSCLC; Insulin signaling; Lung cancer; IRS proteins. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100015 |
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| Mahasneh,Amjad; Jubaili,Amal; El Bateiha,Ahmed; Al-Ghazo,Mohammad; Matalka,Ismail; Malkawi,Mousa. |
| The arylamine N-acetyltransferase 2 (NAT2) enzymes detoxify a wide range of naturally occurring xenobiotics including carcinogens and drugs. Point mutations in the NAT2 gene result in the variant alleles M1 (NAT2 *5A), M2 (NAT2*6A), M3 (NAT2*7) and M4 (NAT2 *14A) from the wild-type WT (NAT2 *4) allele. The current study was aimed at screening genetic polymorphisms of NAT2 gene in 49 lung cancer patients, 54 colorectal cancer patients and 99 cancer-free controls, using PCR-RFLP. There were significant differences in allele frequencies between lung cancer patients and controls in the WT, M2 and M3 alleles (p < 0.05). However, only M2 and M3 allele frequencies were different between colorectal cancer patients and controls (p < 0.05). There was a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: NAT2; Lung cancer; Colorectal cancer; Polymorphisms. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500003 |
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| Ljujic,Mila; Topic,Aleksandra; Nikolic,Aleksandra; Divac,Aleksandra; Grujic,Milan; Mitic-Milikic,Marija; Radojkovic,Dragica. |
| The alpha-1-antitrypsin (A1AT) gene is highly polymorphic, with more than 100 genetic variants identified of which some can affect A1AT protein concentration and/or function and lead to pulmonary and/or liver disease. This study reports on the characterization of a p.G320R variant found in two patients, one with emphysema and the other with lung cancer. This variant results from a single base-pair substitution in exon 4 of the A1AT gene, and has been characterized as P by isoelectric focusing. Functional evaluation of the A1AT p.G320R variant was through comparing specific trypsin inhibitory activity in two patients with pulmonary disorders, carriers of the p.G320R variant, and 19 healthy individuals, carriers of normal A1AT M variants. Results showed that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alpha-1-antitrypsin; Emphysema; Lung cancer; P variant; Specific trypsin inhibitory activity. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100002 |
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| Chen,Yong; Shi,Jian-Xin; Pan,Xu-Feng; Feng,Jian; Zhao,Heng. |
| Over the past three decades, mortality from lung cancer has sharply and continuously increased in China, ascending to the first cause of death among all types of cancer. The ability to identify the actual sequence of gene mutations may help doctors determine which mutations lead to precancerous lesions and which produce invasive carcinomas, especially using next-generation sequencing (NGS) technology. In this study, we analyzed the latest lung cancer data in the COSMIC database, in order to find genomic "hotspots" that are frequently mutated in human lung cancer genomes. The results revealed that the most frequently mutated lung cancer genes are EGFR, KRAS and TP53.In recent years, EGFR and KRAS lung cancer test kits have been utilized for detecting lung... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lung cancer; Next-generation sequencing; Somatic mutation kit; COSMIC. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300022 |
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| Elkady,Ayman I.. |
| There is an urgent need to improve the clinical management of non-small cell lung cancer (NSCLC), one of the most frequent causes of cancer-related deaths in men and women worldwide. Rhazya stricta, an important medicinal plant used in traditional Oriental medicine, possesses anti-oxidant, anti-carcinogenic and free radical scavenging properties. This study was done to explore the potential anticancer activity of a crude alkaloid extract of R. stricta (CAERS) against the NSCLC line A549. CAERS markedly suppressed the growth of A549 cells and considerably enhanced the anti-proliferative potential of cisplatin. CAERS-mediated inhibition of A549 cell growth correlated with the induction of apoptosis that was accompanied by numerous morphological changes, DNA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apoptosis; Lung cancer; Medicinal plant; RT-PCR; Western blot. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100003 |
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| Maillet, A; Congy Jolivet, N; Le Guellec, S; Vecellio, L; Hamard, S; Courty, Y; Courtois, Anthony; Gauthier, F; Diot, P; Thibault, G; Lemarie, E; Heuze Vourc' H, N. |
| Purpose. Despite an increasing interest in the use of inhalation for local delivery of molecules for respiratory diseases and systemic disorders, methods to deliver therapy through air-ways has received little attention for lung cancer treatment. However, inhalation of anticancer drugs is an attractive alternative route to systemic administration which results in limited concentration of the medication in the lungs, and triggers whole-body toxicity. In this study, we investigated the feasibility of nebulization for therapeutic antibodies, a new class of fully-approved anticancer drugs in oncology medicine. Materials and methods. Cetuximab, a chimeric IgG1 targeting the epidermal growth factor receptor (EGER), was nebulized using three types of delivery... |
| Tipo: Text |
Palavras-chave: Nebulizer; Lung cancer; Cetuximab; Anticancer antibody; Aggregation; Aerosol. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4201.pdf |
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| SCHNEIDER,MARCIA R.; SILVA,ANDRÉA L.G. DA; GOULART,CÁSSIA L.; SCHNEIDERS,PALOMA B.; WEBER,AUGUSTO F.; POSSUELO,LIA G.; VALIM,ANDREIA R.M.. |
| Abstract: This study evaluated 24 patients with lung cancer (CA) and 23 individuals with no smoking history or cancer in the family and without respiratory disease in childhood (CO). Peripheral blood lymphocytes was used to perform alkaline comet assay and to assess DNA damage as well as to evaluate methyl methane sulfonate (MMS) DNA repair after one hour and three hours at 37 ºC. The percentage of residual damage (RD) after three hours of MMS treatment, for each patient was assessed. The majority of patients were in the CA group, male patients, former smokers, with a history of smoking for 15 years and without associated comorbidities. Alkaline and residual damages were higher in the CA group when compared to controls (alkaline damage P = 0.015 and RD P =... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Comet assay; DNA damage; DNA repair; Lung cancer. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500806 |
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| Adonis,Marta I; Díaz,Jose; Miranda,Veronica R; Chahuan,Marco; Zambrano,Alcides; Benitez,Hugo C; Campos,Monica; Avaria,Pablo; Urzúa,Ulises; Marín,Pedro; Gohurdett,Mariela; Cisterna,Yasna; Gil,Lionel. |
| BACKGROUND: The mortality of lung cancer (LC), increases each year in the world, in spite of any advances, in development of new drugs to advance stages of LC. The high incidence of LC has been associated with smoking habit, genetic diversity and environmental pollution. Antofagasta region has been reported to have the highest LC mortality rate in Chile and its inhabitants were exposed to arsenic in their drinking water in concentrations as high as 870 μg/L. Non-invasive techniques such as biomarkers (Automatic Quantitative Cytometry: AQC and DR70) and Auto Fluorescence Bronchoscopy (AFB) might be potentially useful as a supplementary diagnostic approach and early detection. Early detection is one of the most important factors to intervene and... |
| Tipo: Journal article |
Palavras-chave: Early detection; Biomarkers; Lung cancer. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100056 |
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| Yang,Zhen; Jiang,DanDan; Zhu,Qiang; Xiao,BinBin; Chen,Liang An. |
| ABSTRACT DNA vaccines have been shown to be an effective approach to induce antigen-specific cellular and humoral immunity. However, the inability of DNA vaccines to elicit strong immune responses in clinical trials limits the application of DNA vaccines. Here, we developed a new DNA vaccine based on MUC1, which has been suggested as a potential target for lung cancer therapy, and we enhanced the potency of the DNA vaccine by including granulocyte-macrophage colony-stimulating factor (GM-CSF) as an adjuvant. A series of DNA plasmids encoding MUC1, human GM-CSF and their conjugates were constructed and injected into female mice intramuscularly (i.m.). This action was followed by an electric pulse. The humoral and cellular immune responses after immunization... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MUC1; GM-CSF; Lung cancer; DNA vaccine. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100331 |
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| Weng,K.-G.; Yuan,Y.-L.. |
| Series of novel coumarin derivatives [I (a–d) and II (a–d)] were successfully synthesized and their structures were determined based on infrared 1H-nuclear magnetic resonance (NMR), HRMS, and single crystal X-ray crystallography. Additionally, the new synthesized compounds were evaluated to identify the molecular characteristics that contribute to their cytotoxicity, which was tested against SK-LU-1, SPC-A-1 and 95D human lung cancer cell lines, using the MTT assay. The results of this study showed that compounds Ic, Id, IIc, and IId exhibited an efficient percentage of inhibition of cell proliferation. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coumarin; X-ray; Lung cancer. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017001100603 |
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| Rangel,M.P.; Antonangelo,L.; Acencio,M.M.P.; Faria,C.S.; de Sá,V.K.; Leão,P.S.; Farhat,C.; Fabro,A.T.; Longatto Filho,A.; Reis,R.M.; Takagaki,T.; Capelozzi,V.L.. |
| Cofilin-1 (CFL1), a small protein of 18 kDa, has been studied as a biomarker due to its involvement in tumor cell migration and invasion. Our aim was to evaluate CFL1 as an indicator of malignancy and aggressiveness in sputum samples. CFL1 was analyzed by ELISA immunoassay in the sputum of 73 lung cancer patients, 13 cancer-free patients, and 6 healthy volunteers. Statistical analyses included ANOVA, ROC curves, Spearman correlation, and logistic regression. Sputum CFL1 levels were increased in cancer patients compared to cancer-free patients and volunteers (P<0.05). High expression of sputum CFL1 was correlated to T4 stage (P=0.01) and N stage (P=0.03), tobacco history (P=0.01), and squamous cell carcinoma histologic type (P=0.04). The accuracy of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lung cancer; Cofilin-1; Sputum; Liquid-biopsy; Metastases; Biomarker. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000800603 |
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| Rangel,M.P.; de Sá,V.K.; Martins,V.; Martins,J.R.M.; Parra,E.R.; Mendes,A.; Andrade,P.C.; Reis,R.M.; Longatto-Filho,A.; Oliveira,C.Z.; Takagaki,T.; Carraro,D.M.; Nader,H.B.; Capelozzi,V.L.. |
| Hyaluronan (HA) shows promise for detecting cancerous change in pleural effusion and urine. However, there is uncertainty about the localization of HA in tumor tissue and its relationship with different histological types and other components of the extracellular matrix, such as angiogenesis. We evaluated the association between HA and degree of malignancy through expression in lung tumor tissue and sputum. Tumoral tissue had significantly increased HA compared to normal tissue. Strong HA staining intensity associated with cancer cells was significant in squamous cell carcinoma compared to adenocarcinoma and large cell carcinoma. A significant direct association was found between tumors with a high percentage of HA and MVD (microvessel density) in tumoral... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hyaluronic acid; Sputum; Lung cancer; Immunohistochemistry; ELISA. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000600557 |
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| Sá,V.K. de; Rocha,T.P.; Moreira,AL.; Soares,F.A.; Takagaki,T.; Carvalho,L.; Nicholson,A.G.; Capelozzi,V.L.. |
| We collected a series of 136 lung/bronchial and 56 matched lung parenchyma tissue samples from patients who underwent lung/bronchial biopsies and presented invasive carcinoma after lung surgery. The lung/bronchial samples included basal cell hyperplasia, squamous metaplasia, moderate dysplasia, adenomatous hyperplasia, severe dysplasia, squamous cell carcinoma and adenocarcinoma. Matched lung parenchyma tissue samples included 25 squamous cell carcinomas and 31 adenocarcinomas. Immunohistochemistry was performed to analyze for the distribution of hyaluronidase (Hyal)-1 and −3, and hyaluronan synthases (HAS)-1, −2, and −3. Hyal-1 showed significantly higher expression in basal cell hyperplasia than in moderate dysplasia (P=0.01), atypical adenomatous... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hyaluronidases and hyaluronan synthases; Lung cancer; Pre-neoplastic lung/bronchial lesions; Immunohistochemistry; Morphometry; Prognosis. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001101039 |
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| Cao,Chengsong; Liu,Yong; Wang,Qun; Zhao,Jing; Shi,Ming; Zheng,Junnian. |
| Lung cancer is the most common malignancy worldwide and is characterized by rapid progression, aggressive behavior, frequent recurrence, and poor prognosis. The TCGA database indicates that chondroitin polymerizing factor (CHPF) is overexpressed in human lung cancer tissues compared with normal tissues and this overexpression corresponds to shorter overall survival in lung cancer patients. In this study, to investigate the function of CHPF in lung cancer, lentiviral vectors expressing CHPF shRNA were stably transduced into A549 and H1299 cells. Compared to shCtrl cells, CHPF knockdown cells had significantly reduced proliferation. Furthermore, the silencing of CHPF in A549 and H1299 cells resulted in apoptotic induction, which led to decreased colony... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CHPF; Lung cancer; Proliferation; Invasion; TCGA; Affymetrix Gene Chip. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000500610 |
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| Registros recuperados: 25 | |
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