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玄, 学南; XUAN, XUENAN. |
本研究では、プロテオミックス法によるネオスポラ原虫の全虫体タン パク質の網羅的解析を行った。MALDI-TOF-MS 解析により約600 種類の可視化されたタンパク質 の中から約60 種類のタンパク質の同定に成功した。そのうちの一部は原虫の宿主細胞への吸 着・侵入ならびにエネルギー代謝に関わるタンパク質であることが示唆された。これらの成果 は、ネオスポラ原虫の新規ワクチン・治療薬開発に繋がるものとして期待される。 The aims of this study were to widen our view on the global expression of protein profile of Neospora caninum parasite and to discover vaccine and drug target molecules for control of neosporosis. Approximately 600 protein spots were detected on 2-DE, and 60 proteins were identified by MALDI-TOF-MS analysis. Some of the identified proteins were predicted to be involved in the invasion of the parasite into the host cells and in parasite metabolism. Therefore, some of these proteins could be potential vaccine candidates and drug targets. |
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Palavras-chave: ネオスポラ; トキソプラズマ; プロテオミックス; ワクチン; 治療薬; MALDI-TOF-MS; 抗原; 酵素. |
Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2759 |
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Santos,Leonardo Silva; Soriano,Maria del Pilar Camarantin; Mirabal-Gallardo,Yaneris; Carrasco-Sanchez,Veronica; Nachtigall,Fabiane Manke; Pereira,Iris; Pereira,Eugenia. |
The aim of this work was to study a fast, new, sensitive, and simple method for the chemotaxonomic classification of Chilean lichens (Teloschistes chrysophthalmus, Ramalina farinacea, Usnea pusilla, Ramalina chilensis and Stereocaulon ramulosum) using MALDI-TOF-MS and UPLC-ESI(-)-MS data. Lichens soluble proteins fingerprints were acquired by MALDI-TOF-MS and they were analyzed by chemometric (PCA). Lichens organic extracts fingerprints were obtained by UPLC-ESI(-)-MS. MALDI-TOF-MS associated with chemometric analysis was used to detect new m/z patterns of soluble proteins that were compared with Protein Data Bank of UnitPro. These data also permitted the satisfactory distinction among the families and species. UPLC-ESI(-)-MS fingerprints analyses of the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chilean lichens; Chemotaxonomic; Fingerprint; MALDI-TOF-MS; UPLC-ESI(-)-MS. |
Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000200244 |
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