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Role of PPase-SE in Geotrichum klebahnii, a yeast-like fungus able to solubilize pectin Electron. J. Biotechnol.
Rojas,Natalia Lorena; Cavalitto,Sebastián Fernando; Mignone,Carlos Fernando; Hours,Roque Alberto.
Protopectinases (PPases) constitute a heterogeneous group of extracellular enzymes able to release soluble pectin from insoluble protopectin in plant tissues. Geotrichum klebahnii (ATCC 42397) produces PPase-SE with endopolygalacturonase activity. PPase-SE has been used for pectin extraction and maceration of plant tissues. Here, the capacity of G. klebahnii to use different pectins as carbon and energy sources (CES) was studied, in addition to PPase-SE capacity to release pectin from lemon peel. The strain was unable to use pectin from different origins as CES. When G. klebahnii was cultivated with mixtures of different amounts of glucose and citrus pectin as CES, the biomass obtained was proportional to the initial concentration of glucose, which was...
Tipo: Journal article Palavras-chave: Carbon balance; Enzymatic pectin extraction; Microbial pectin utilization; Pectinases; Synthetic medium.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100012
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