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Registros recuperados: 11
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ANTIFUNGAL ACTIVITY OF NEEM (Azadirachta indica: MELIACEAE) EXTRACTS AGAINST DERMATOPHYTES Acta biol.Colomb.
OSPINA SALAZAR,Daniel Iván; HOYOS SÁNCHEZ,Rodrigo Alberto; OROZCO SÁNCHEZ,Fernando; ARANGO ARTEAGA,Myrtha; GÓMEZ LONDOÑO,Luisa Fernanda.
In order to assess the antifungal activity of methanolic extracts from neem tree (Azadirachta indica A. Juss.), several bioassays were conducted following M38-A2 broth microdilution method on 14 isolates of the dermatophytes Trichophytonmentagrophytes, Trichophyton rubrum, Microsporum canis and Epidermophyton floccosum. Neem extracts were obtained through methanol-hexane partitioning of mature green leaves and seed oil. Furthermore, high performance liquid chromatography (HPLC) analyses were carried out to relate the chemical profile with their content of terpenoids, ofwidely known antifungal activity. The antimycotic Terbinafine served as a positive control. Results showed that there was total growth inhibition of the dermatophytes isolates at minimal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: HPLC; Neem; Microbial sensitivity tests; Minimum inhibitory concentration; Terpenoids.
Ano: 2015 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000300017
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Effect of shading and nitrogen fertilization on nitrogen metabolism, essential oil content and antimicrobial activity of Achillea millefolium Biological Sciences
Lima, Milene Conceição; da-Silva, Cristiane Jovelina; Mariot, Marcio Paim; Freitag, Rogério Antônio; Serpa, Rosana; Ribeiro, Gladis Aver; Amarante, Luciano do.
The Achillea millefolium L. is a perennial herb with important antibacterial, antifungal, anti-inflammatory, antitumoral, and antioxidant properties. This research aimed to investigate the effect of shading (75%; black net) and nitrogen fertilization (0, 75 and 150 kg urea ha-1) on the nitrogen metabolism, essential oil yield and antimicrobial activity of A. millefolium at vegetative- and reproductive-stage. The evaluated parameters varied depending on the organ and the phenological stage of the plant considered. Overall, our findings indicated that shading decreased nitrogen assimilation. Decreased activities of nitrate reductase and glutamine synthetase were observed on shaded plants during reproductive and vegetative stages, respectively. Nitrate and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Light reduction; Medicinal plants; Minimum inhibitory concentration; Nitrogen assimilation; Yarrow.
Ano: 2020 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/46412
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Multidrug-resistant bacteria isolated from intensive-care-unit patient samples BJID
Japoni,Aziz; Vazin,Afsaneh; Hamedi,Mahdi; Davarpanah,Mohammad Ali; Alborzi,Abdolvahab; Rafaatpour,Noraladin.
We examined epidemiological aspects and bacterial resistance patterns of bacteria isolated from intensive care unit (ICU) patient samples. During a 10 month period (from June 2006 to March 2007), 812 samples of blood, urine and cerebral spinal fluid (CSF) from 553 hospitalized patients, in ICU wards, including pediatric surgical, neonatal, adult surgical I, adult surgical II, general pediatrics, neurosurgical I, neurosurgical II, and internal medical, were collected. Minimum inhibitory concentration (MIC) of antibiotics for bacteria isolates was determined by the E-test method. The internal medicine ICU with 28.7% admissions gave the largest contribution. Coagulase negative staphylococci at frequencies of 66.7 % and 36.5 % and E. coli at 20.9% were the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Intensive care unit; Minimum inhibitory concentration; Multidrug-resistant bacteria.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000200009
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Biological activities of a mixture of biosurfactant from Bacillus subtilis and alkaline lipase from Fusarium oxysporum BJM
Quadros,Cedenir Pereira de; Duarte,Marta Cristina Teixeira; Pastore,Gláucia Maria.
In this study, we investigate the antimicrobial effects of a mixture of a biosurfactant from Bacillus subtilis and an alkaline lipase from Fusarium oxysporum (AL/BS mix) on several types of microorganisms, as well as their abilities to remove Listeria innocua ATCC 33093 biofilm from stainless steel coupons. The AL/BS mix had a surface tension of around 30 mN.m-1, indicating that the presence of alkaline lipase did not interfere in the surface activity properties of the tensoactive component. The antimicrobial activity of the AL/BS mix was determined by minimum inhibitory concentration (MIC) micro-assays. Among all the tested organisms, the presence of the mixture only affected the growth of B. subtilis CCT 2576, B. cereus ATCC 10876 and L. innocua. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial activity; Listeria innocua; Microbial biofilm; Minimum inhibitory concentration; Stainless steel surface.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100045
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Antifungal susceptibility of clinical and environmental Cryptococcus neoformans and Cryptococcus gattii isolates in Jabalpur, a city of Madhya Pradesh in Central India BJM
Gutch,Ruchi Sethi; Nawange,Shesh Rao; Singh,Shankar Mohan; Yadu,Ruchika; Tiwari,Aditi; Gumasta,Richa; Kavishwar,Arvind.
In this study, we present antifungal susceptibility data of clinical and environmental isolates of Central Indian Cryptococcus neoformans (Serotype A, n = 8 and n = 50 respectively) and Cryptococcus gattii (Serotype B, n = 01 and n = 04 respectively). Susceptibilities to fluconazole, itraconazole and ketoconazole were determined by using NCCLS broth micro-dilution methodology. The total number of resistant strains for fluconazole in case of C. neoformans and C. gattii showed a significant difference by using chi-square test (p < 0.05*), while considering fisher's exact p value was nonsignificant (p > 0.05). However, the total number of resistant strains for itraconazole and ketoconazole was not found statistically significant. A comparison of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cryptococcus neoformans; Cryptococcus gattii; Minimum inhibitory concentration; Azoles; Central India.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401125
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Antibacterial susceptibility patterns and cross-resistance of methicillin resistant and sensitive Staphyloccus aureus isolated from the hospitalized patients in Shiraz, Iran BJM
Japoni,Aziz; Ziyaeyan,Mazyar; Jmalidoust,Marzieh; Farshad,Shohreh; Alborzi,Abdolvahab; Rafaatpour,Noraladdin; Badiee,Parisa.
Nosocomial infections caused by methicillin-resistant staphylococci (MRSA) pose a serious problem in many countries. This study aimed to determine the antibacterial susceptibility patterns of methicillin sensitive and resistant Staphylococcus aureus isolates from the hospitalized patients. Totally 356 isolates of Staphylococcus aureus (S. aureus) including 200, 137 and 19 corresponding to MSSA, MRSA, and intermediate MRSA strains, respectively were isolated. Antibacterial susceptibility patterns of the isolates to 14 antibiotics were examined using Kirby-Bauer method. MICs of 15 antibiotics to 156 MRSA isolates were determined by E test method. Cross-resistances of MRSA isolates (137+19) to the other tested antibiotics were also determined. S.aureus with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MRSA; Minimum inhibitory concentration; Empirical therapy.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300005
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Screening methods to determine antibacterial activity of natural products BJM
Valgas,Cleidson; Souza,Simone Machado de; Smânia,Elza F A; Smânia Jr.,Artur.
The emergence of new infectious diseases, the resurgence of several infections that appeared to have been controlled and the increase in bacterial resistance have created the necessity for studies directed towards the development of new antimicrobials. Considering the failure to acquire new molecules with antimicrobial properties from microorganisms, the optimization for screening methods used for the identification of antimicrobials from other natural sources is of great importance. The objective of this study was to evaluate technical variants used in screening methods to determine antibacterial activity of natural products. Thus, a varied range of natural products of plant, fungi and lichen origin were tested against two bacterial species,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agar diffusion; Antimicrobial activity; Bioautographic methods; Minimum inhibitory concentration; Minimum bactericidal concentration; Natural products.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200034
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Antimicrobial activity assessment of time-dependent release bilayer tablets of amoxicillin trihydrate BJPS
Beg,Sarwar; Nayak,Amit Kumar; Kohli,Kanchan; Swain,Suryakanta; Hasnain,MS.
The aim of present study was the assessment of antimicrobial activity of prepared time-dependent release bilayer tablets of amoxicillin trihydrate and in vitro evaluation of drug release by antimicrobial assay using agar plate diffusion method. The bilayer tablets comprised of a delayed and sustained release layer. Direct compression method was used for the preparation of bilayer tablets containing Eudragit-L100 D55 as delayed release polymer, and HPMCK4M and HPMCK15 as sustained release polymers. The prepared bilayer tablets containing amoxicillin trihydrate were evaluated for hardness, thickness, friability, weight variation and drug content. Further, in vitro drug release was assessed by antimicrobial assay using S. aureus and E. coli as test...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chronotherapeutics; Time-dependent release; In vitro dissolution; Minimum inhibitory concentration; Zone of inhibition.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502012000200010
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Susceptibility profile of Brazilian Rhodococcus equi isolates against azithromycin, clarithromycin and erythromycin Ciência Rural
Gressler,Letícia Trevisan; Silveira,Bibiana Petri da; Schwab,Marcelo Luís; Vargas,Agueda Castagna de; Pötter,Luciana; Botton,Sônia de Avila.
Rhodococcus equi infection treatment is usually a macrolide (azithromycin - AZM, clarithromycin - CLR and erythromycin - ERY) and rifampicin combination. However, resistance cases have been reported, especially for ERY. In view of the need of a study about Brazilian isolates susceptibility profile, this study aimed to characterize the minimum inhibitory concentration (MIC) of the macrolides - AZM, CLR and ERY - against 44 R. equi isolates. It was found all isolates CLR and AZM sensitive; however, for ERY, 27% (12/44) were classified as intermediate sensitivity. R. equi Brazilian isolates used here showed a large susceptibility profile, except against ERY, for which it was observed some resistance evidence. In order to avoid failures in the equine...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Macrolide; Minimum inhibitory concentration; Antimicrobial susceptibility; Rhodococcosis.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000400680
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In vitroantibacterial effect of wasp (Vespa orientalis) venom J. Venom. Anim. Toxins incl. Trop. Dis.
Jalaei,Jafar; Fazeli,Mehdi; Rajaian,Hamid; Shekarforoush,Seyed Shahram.
Background The emergence of antibacterial resistance against several classes of antibiotics is an inevitable consequence of drug overuse. As antimicrobial resistance spreads throughout the globe, new substances will always be necessary to fight against multidrug-resistant microorganisms. Venoms of many animals have recently gained attention in the search for new antimicrobials to treat infectious diseases. Thefore, the present study aimed to study the antibacterial effects of wasp (Vespa orientalis) crude venom. Two gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis) and two gram-negative ones (Escherichia coli and Klesiella pneumonia) were compared for their sensitivity to the venom by determining the inhibition zone (Kirby-Bauer method)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wasp venom; Vespa orientalis; Antimicrobial activity; Minimum inhibitory concentration.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200329
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Antibacterial effect of crude methanol Carica papaya L. (papaya) extract and amoxicillin combination Plantas Medicinales
Bridge,Mwesigwa; Dominguez Montero,Genny; Betancourt Valladares,Miriela; Katawera,Victoria; Nkwangu,David; Oweta Noah,Joseph Openy.
Tipo: Journal article Palavras-chave: Additive interaction; Carica papaya; Amoxicillin; Minimum inhibitory concentration; Fractional inhibitory concentration index.
Ano: 2015 URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1028-47962015000400009
Registros recuperados: 11
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