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| Registros recuperados: 34 | |
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| Barros,Aline F; Campos,Vicente P; Souza,Larissa N; Costa,Sarah S; Terra,Willian C; Lessa,Josimar HL. |
| ABSTRACT Species of the genus Meloidogyne are limiting factors in vegetable crop production. Studies in Brazil about the occurrence of root-knot nematodes in areas of vegetable crop growth have been conducted without using advanced techniques. Using modern techniques, such as biochemical and molecular methods, improves the accuracy of Meloidogyne species identification. The present study characterized species of Meloidogyne in 36 samples associated with vegetable crops using isoenzyme electrophoresis, SCAR markers, and morphological markers, in addition to validating SCAR markers for accurate species identification. The species M. incognita, M. javanica, M. hapla, M. morocciensis, and M. arenaria were identified, with the first two being the most frequent.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Meloidogyne; Diagnosis; Molecular biology; Vegetable crops. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362018000400473 |
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| Comabella Soto, Y.; Centro de Investigaciones Marinas Universidad de La Habana. |
| En el presente estudio se evalúa el efecto que la levadura Saccharomyces cerevisiae de forma desintegrada (LD) y tres fracciones obtenidas de ella (complejo pared-membrana (FP), núcleo- proteínas (FN) y citoplasma (FC)) produce en dos modelos biológicos experimentales: la línea celular MB16-F10 y la especie Artemia franciscana. Para los estudios celulares se empleó la parte soluble de cada una de estas fracciones probándose las concentraciones de 0.5, 1, 2, 5 y 10 µg/mL adicionándose el valor de 25 µg/mL para LD y FC. Se determinó el efecto en la proliferación, vitalidad y fases del ciclo celular a las 48 y 72 horas. Como resultado se obtuvo una acción estimulante de la proliferación celular con LD 5 µg/mL a las 48 horas y con FN 0.5 y 1 µg/mL a... |
| Tipo: Theses and Dissertations |
Palavras-chave: Molecular biology; Cell biology. |
| Ano: 2003 |
URL: http://hdl.handle.net/1834/5666 |
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| Calegario,Renata Faier; Ferreira,Sávio de Siqueira; Andrade,Eduardo Chumbinho de; Zerbini,Francisco Murilo. |
| The objective of this work was the biological and molecular characterization of a begomovirus detected in São Joaquim de Bicas, Minas Gerais, Brazil, named TGV-[Bi2], by determining its host range, complete nucleotide sequence and phylogenetic relationships with other begomoviruses. Biological characterization consisted of a host range study using either sap inoculation or particle bombardment as inoculation methods. The yellow spot virus can infect plants in Solanaceae and Amaranthaceae, including economically importat crops as sweet pepper, and weeds as Datura stramonium and Nicotiana silvestris. For the molecular characterization, the full-length genome (DNA-A and DNA-B) was amplified, cloned and completely sequenced. Sequence comparisons and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lycopersicon esculentum; Bemisia tabaci; ToYSV; Molecular biology; Geminivirus; Whitefly. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000900016 |
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| Coelho,LR; Melo,RT; Monteiro,GP; Reischak,D; Mendonça,AO; Tavares,AAS; Rossi,DA. |
| ABSTRACT The analysis of Salmonella in the feces and the birds’ environment is a way of monitoring the colonization in the flocks and verifying the need for the introduction of stricter controls, in such a way that the results of the tests should be known before being sent for slaughter. The polymerase chain reaction (PCR), as well as other rapid methods represent alternatives increasingly used to detect enteric pathogens, but they need proof of effectiveness for their wide use. The aim of this study was to evaluate the equivalence between the results obtained by the methods: real-time PCR (BAX® System), Modified Rappaport-Vassiliadis Semi-solid Medium (MSRV) (ISO 6579) and the traditional method of official reference in Brazil for research of S.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BAX® System; MSRV; Salmonella Enteritidis; S. Typhimurium; Molecular biology. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000200303 |
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| CORDOVA,CAIO M.M.; HOELTGEBAUM,DANIELA L.; MACHADO,LAÍS D.P.N.; SANTOS,LARISSA DOS. |
| ABSTRACT Mycoplasmas are a large group of bacteria, sorted into different genera in the Mollicutes class, whose main characteristic in common, besides the small genome, is the absence of cell wall. They are considered cellular and molecular biology study models. We present an updated review of the molecular biology of these model microorganisms and the development of replicative vectors for the transformation of mycoplasmas. Synthetic biology studies inspired by these pioneering works became possible and won the attention of the mainstream media. For the first time, an artificial genome was synthesized (a minimal genome produced from consensus sequences obtained from mycoplasmas). For the first time, a functional artificial cell has been constructed by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycoplasma; Molecular biology; Microbial genetics; Synthetic biology; Gene transfer techniques. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200599 |
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| Meneghini,Rogério; Gamba,Estêvão C.. |
| Several genome sequencing programs were launched in Brazil by the end of the nineties and the early 2000s.The most important initiatives were supported by the ONSA program (http://watson.fapesp.br/onsa/Genoma3.htm) and aimed at gaining domain in genomic technology and bringing molecular biology to the state of art. Two mainsets of data were collected in the 1996-2007 period to evaluate the results of these genome programs: the scientific production (Scopus and Web of Science databases) and the register of patents (US Patent and Trademark Office), both related to the progress of molecular biology along this period. In regard to the former, Brazil took a great leap in comparison to 17 other developed and developing countries, being only surpassed by China.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Science assessment; Genomics; Scientometrics; Molecular biology. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000200031 |
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| Souza,D.M.B.; Barros,M.G.O.; Silva,J.S.C.; Silva,M.B.; Coleto,Z.F.; Jimenez,G.C.; Adrião,M.; Wischral,A.. |
| Fifteen female canines with mammary tumors and 6 normal females were used to study mutations in exons 4 to 8 of the p53 gene. DNA samples from the tumors, respective adjacent normal mammary tissue and mammary glands from healthy animals were sequenced and analyzed for the presence of mutations. Mutations were found in 71.8% of the samples and the most frequent were missense mutations. The most attacked exons in the mammary tumor were 5, 7 and 8, with 23.4, 31.6 and 23.4% mutations, respectively. Canine mammary tumors are related to mutations in gene p53 and mutations mostly occur in the region of the protein that is linked to the DNA in the cell nucleus, which can change the functionality of the cell and propitiate tumor growth. Despite being... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bitch; DNA; Mamma; Molecular biology; Neoplasm; PCR. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352012000200013 |
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| Registros recuperados: 34 | |
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