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Benabdelmouna, Abdellah; Ledu, Christophe. |
We describe two methods of producing viable and fertile autotetraploid Pacific oyster (Crassostrea gigas Thunberg) based on the use of normal-sized oocytes produced by normal diploid females. Our methods showed that the oocyte size is not a limiting factor for the success of the induction to autotetraploidy. These methods offer means of direct introgression of genetic progress from elite diploid lines to tetraploids used as broodstock, avoiding a triploid step with the risk of transferring undesirable traits from highly fecund triploids. High variability in the level of cytogenetic stability was found among the different tetraploid oysters tested showing that induction method has an important impact on the long-term cytogenetic stability of the... |
Tipo: Text |
Palavras-chave: Oyster; Tetraploid induction; Mosaicism; Aneuploidy; Cytogenetic stability. |
Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00278/38924/37464.pdf |
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Carvalho, Thaís Lima; UNESP - IBB; Curi, Rogério Abdallah; UNESP - FMVZ; Santiloni, Valquíria; UNESP - IBB; Chieregatto, Cleyde Angélica Ferreira da Silva; Sociedade dos Zoológicos do Brasil; Rocha, Guaracy Tadeu; UNESP - IBB; Mota, Lígia Souza Lima Silveira da; UNESP - IBB. |
The bush dog (Speothos venaticus) is a South American canid, included in the IBAMA (Brazilian Institute of Environment and Renewable Natural Resources) official list of animals threatened with extinction, in the vulnerable category. As a preservation and conservation strategy, specimens kept in captivity by Brazilian Institutions are monitored by a management plan. In order to characterize and analyze the genetic variability of bush dog specimens, a cytogenetic analysis was carried out, and microsatellite data were also obtained through the use of 15 primers, originally developed for the domestic dog (Canis familiaris). All tested primers showed transferability and amplified fragment sizes similar to those described for the canine genome. From the total... |
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Palavras-chave: 2.02.04.00-0 bush dog; Canids; Chromosome; Microsatellite; Mosaicism Genética Animal bush dog; Canids; Chromosome; Microsatellite; Mosaicism. |
Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6775 |
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