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| Wickert,Ester; Marcondes,Jackson; Lemos,Manoel Victor; Lemos,Eliana G.M.. |
| Assimilation of nitrate and ammonium are vital procedures for plant development and growth. From these primary paths of inorganic nitrogen assimilation, this metabolism integrates diverse paths for biosynthesis of macromolecules, such as amino acids and nucleotides, and the central intermediate metabolism, like carbon metabolism and photorespiration. This paper reports research performed in the CitEST (Citrus Expressed Sequence Tag) database for the main genes involved in nitrogen metabolism and those previously described in other organisms. The results show that a complete cluster of genes involved in the assimilation of nitrogen and the metabolisms of glutamine, glutamate, aspartate and asparagine can be found in the CitEST data. The main enzymes found... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nitrogen assimilation; Citrus; CitEST datamining. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500009 |
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| ANDRADE, S. R. M.; SANT'ANNA, J.; MOSQUIM, P. R.. |
| O objetivo deste trabalho foi estudar os efeitos da nutricao nitrica e amoniacal na distribuicao de nitrato e na atividade in vitro da redutase do nitrato em plantas de capim coloniao ( Panicum maximum Jacq.). as plantas receberam 7 mM de nitrogenio nas forma N- NH4 +, N-NO3- ou combinadas nas seguintes proporcoes 0/100, 25/75, 50/50, 75/25 e 100/0. A extracao enzigmatica foi feita em Tris-HCI 50mM pH 7,5, EDTA 0,5 mM e b-mercaptoetanol 10mM, e para o ensaio utilizou-se 200 mmoles de Tris-HCI pH 7,5, 20 mmoles KNO3, 0,1 mmol NADH e 0,1 mL de Triton X-100 1% no volume final de 2,0 mL. A concentracao de nitrato foi levemente superior na parte aerea quando as plantas receberam ambas as formas de nitrogenio, e na raiz seta decresceu com o aumento da proporcao... |
| Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E) |
Palavras-chave: Assimilacao de nitrogenio; Amonio; Nitrogen assimilation; Nitrate; Ammonium; Fisiologia vegetal: Nutricao mineral.; Capim Colonião; Cerrado; Fisiologia Vegetal; Nitrogênio; Nitrato; Panicum Maximum.; Plant physiology.. |
| Ano: 2001 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/567071 |
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| Hernández-Cruz,AE; Sánchez,E; Preciado-Rangel,P; García-Bañuelos,M L; Palomo-Gil,A; Espinoza-Banda,A. |
| In the production of cotton (Gossypium hirsutum L.), nitrogen fertilization is one of the most costly crop practices, but important to reach high yields. However, high nitrogen (N) content in plants does not always translate into a high fibre production. One way of assessing the efciency of the N fertilizer is through the enzymatic activity of the nitrate reductase (NR). This is a key enzyme in N assimilation, whose activity is regulated by a number of endogenous and exogenous factors that determine yield. The aim of this study was to assess the efect of N fertilization on yield, fibre quality, biomass, and NR enzymatic activity in vivo in the cotton variety Fiber Max 989. The evaluated application rates were 0, 50, 100, and 150 kg/ha of N, using urea as a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gossypium hirsutum L; Nitrogen assimilation; Yield. |
| Ano: 2015 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572015000200026 |
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| MAJEROWICZ,NIDIA; PEREIRA,JOSÉ M.S.; MEDICI,LEONARDO O.; BISON,ODAIR; PEREIRA,MAURÍCIO B.; SANTOS JÚNIOR,ULYSSES M.. |
| O uso de fertilizantes, além dos riscos de contaminação ambiental, onera o agricultor, chegando a representar 40% dos custos de produção na cultura do milho. O presente estudo visa identificar características fisiológicas relacionadas com o aumento da eficiência do uso do nitrogênio e assim subsidiar programas de melhoramento genético direcionados para obtenção de genótipos de milho produtivos em solos com baixa disponibilidade de nitrogênio. Foram estudadas as variedades de milho Pedra Dourada, Catetão, Carioca (variedades locais, não melhoradas), BR 106, BR 105 (variedades melhoradas em solos férteis), Nitroflint e Nitrodente (variedades melhoradas em solos pobres em N). Plântulas de milho receberam solução nutritiva de Hoagland modificada quanto às... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nitrogen assimilation; Glutamine synthetase; Nitrate reductase; Photosynthetic pigments; Zea mays L.. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042002000200002 |
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| Carvalho,Isabela Pena Carvalho de; Detmann,Edenio; Paulino,Mário Fonseca; Mantovani,Hilário Cuquetto; Valadares Filho,Sebastião de Campos; Gomes,Daiany Iris; Costa,Viviane Aparecida Carli. |
| Two experiments were carried out to evaluate the effect of supplementation with different nitrogenous compounds on the activities of carboxymethil cellulase (CMCase) and glutamate dehydrogenase (GDH). In the first experiment, four treatments were evaluated in vitro: cellulose, cellulose with casein, cellulose with urea, and cellulose with casamino acids. After 6, 12 and 24 hours of incubation, CMCase and GDH activity, pH, and concentrations of ammonia nitrogen (AN) and microbial protein were measured. In the three incubation periods, the concentration of AN was higher when urea was used as a supplemental source of nitrogen. The activity of CMCase was higher with the addition of urea and casamino acids when compared with the control and the casein... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fibrolytic enzymes; Nitrogen assimilation; Protein effect; Rumen microbiology. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000300031 |
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| Lima, Milene Conceição; da-Silva, Cristiane Jovelina; Mariot, Marcio Paim; Freitag, Rogério Antônio; Serpa, Rosana; Ribeiro, Gladis Aver; Amarante, Luciano do. |
| The Achillea millefolium L. is a perennial herb with important antibacterial, antifungal, anti-inflammatory, antitumoral, and antioxidant properties. This research aimed to investigate the effect of shading (75%; black net) and nitrogen fertilization (0, 75 and 150 kg urea ha-1) on the nitrogen metabolism, essential oil yield and antimicrobial activity of A. millefolium at vegetative- and reproductive-stage. The evaluated parameters varied depending on the organ and the phenological stage of the plant considered. Overall, our findings indicated that shading decreased nitrogen assimilation. Decreased activities of nitrate reductase and glutamine synthetase were observed on shaded plants during reproductive and vegetative stages, respectively. Nitrate and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Light reduction; Medicinal plants; Minimum inhibitory concentration; Nitrogen assimilation; Yarrow. |
| Ano: 2020 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/46412 |
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| Oliveira,I.C.; Brenner,E.; Chiu,J.; Hsieh,M.-H.; Kouranov,A.; Lam,H.-M.; Shin,M.J.; Coruzzi,G.. |
| We are using molecular, biochemical, and genetic approaches to study the structural and regulatory genes controlling the assimilation of inorganic nitrogen into the amino acids glutamine, glutamate, aspartate and asparagine. These amino acids serve as the principal nitrogen-transport amino acids in most crop and higher plants including Arabidopsis thaliana. We have begun to investigate the regulatory mechanisms controlling nitrogen assimilation into these amino acids in plants using molecular and genetic approaches in Arabidopsis. The synthesis of the amide amino acids glutamine and asparagine is subject to tight regulation in response to environmental factors such as light and to metabolic factors such as sucrose and amino acids. For instance, light... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amino acids; Carbon; Ammonium; Nitrogen assimilation; Gene expression; Arabidopsis thaliana. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500003 |
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| Cui,Cui; Xie,Xurong; Wang,Liu-Yan; Wang,Rui-Li; Lei,Wei; Lv,Jun; Chen,Liuyi; Gao,Huan-Huan; Ye,Sang; Huang,Linya; Zhou,Qing-Yuan. |
| ABSTRACT: Herbicide application is an effective weed control method for mitigating crop yield loss; however, herbicide overuse can cause toxicity in non-target plants. The present study evaluated the effects of glufosinate at recommended dose for agricultural application (0.45 kg ha-1) and at overuse dose (0.90 kg ha-1) glufosinate application on photosynthetic performance and nitrogen assimilation of the rapeseed varieties D148 and Zhongshuang 11 (ZS11). Both glufosinate concentrations significantly decreased the content of chlorophyll and nitrogenous compounds, except free proline, and the activity of glutamine synthetase and glutamate synthase, and increased the activity of glutamic acid dehydrogenase in both varieties. When the concentration of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Residual glufosinate; Rapeseed; Seedling transplanted; Photosynthesis; Nitrogen assimilation. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000400251 |
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