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Genotoxic evaluation of sodium nitroprusside in Aspergillus nidulans Genet. Mol. Biol.
Chiuchetta,Simone Jurema Ruggeri; Castro-Prado,Marialba Avezum Alves de.
The exogenous nitric oxide donor, sodium nitroprusside, evaluated the recombinogenic potential of nitric oxide. Drug inhibited mycelial growth and conidiation in A757 Aspergillus nidulans master strain. Two heterozygous diploid strains, one wild (uvsH+//uvsH+) and the other defective to DNA repair (uvsH//uvsH) were used for recombinagenesis tests. Sodium nitroprusside recombinogenic effect was evaluated by the induction of homozygosis of recessive genes, originally present in heterozygous condition. Results show that sodium nitroprusside (40 muM, 80 muM and 160 muM) is effective in inducing mitotic crossing-over in diploid cells of A. nidulans.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Nitric oxide; Parasexual cycle; Homozygotization index; Conidiation; Mitotic recombination.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500023
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Parasexuality in asexual development mutants of Aspergillus nidulans Biol. Res.
ALEXANDRINO BECKER,TÂNIA CRISTINA; ALVES DE CASTRO-PRADO,MARIALBA A.
The parasexual cycle with parameiosis has been characterized previously by the occurrence of genetic recombination and haploidization inside heterokaryotic hyphae prior to conidial formation. The aim of current research was to characterize, through genetic and cytological analyses, an asexual development mutant strain of A. nidulans and to use it to obtain parameiotic segregants. Analyses showed the medusa phenotype of the B84 strain, whose mutant allele was mapped in the chromosome I. The heterokaryons B84(med)//G422(med+) and B84(med)//G839(brl) were formed in liquid MM+2% CM and inoculated in the appropriate selective media. Two mitotic segregant groups were obtained: aneuploids and haploid stable recombinants. Mitotic segregants, wild-types, and...
Tipo: Journal article Palavras-chave: Parasexual cycle; Conidiation mutants; Mitotic recombination; Haploid recombinants.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200012
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Pulsed field gel electrophoresis reveals chromosome length and number differences in Brazilian strains of Metarhizium Anisopliae BABT
Kava-Cordeiro,Vanessa; Queiroz,Marisa Vieira de; Pizzirani-Kleiner,Aline Aparecida; Azevedo,João Lúcio.
Electrophoretic karyotypes of eight wild-type strains of Metarhizium anisopliae var. anisopliae were obtained by pulsed-field gel electrophoresis. These strains were isolated from insects of six different Brazilian states. The chromosomal DNA molecules of three strains were separated into seven bands and of five strains into eight bands. Chromosome length polymorphisms were also observed. The size of the chromosomal DNA of all strains varied between 7.7 and 0.9 Mb using the Aspergillus nidulans chromosomes as size standards. The total genome size of these strains was estimated in at least 29.7 Mb. Some correlations between differences in karyotype and occurrence of parasexual cycle likewise the host specificity were discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metarhizium anisopliae; Pulsed-field gel electrophoresis; Chromosome polymorphisms; Parasexual cycle; Entomopathogenic fungus.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000100001
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